Consistent Expression Research Articles

Improving the Law on International Treaties 2016 from the Perspective of Legal Document Drafting Techniques

The quality of a legal document is affected by its drafting techniques. The Law on International Treaties 2016 was promulgated before the issuance of Resolution No. 351/2017/UBTVQH14 dated March 14, 2017, which stipulates the format and drafting techniques of legal documents. This article analyzes the technical limitations of the Law on International Treaties 2016, including issues related to its structure, the lack of text cohesion, and the inconsistencies in expression and word usage. In addition, the author makes several recommendations to improve drafting techniques of this Law.

Immune checkpoint inhibitors in metastatic NSCLC: challenges and future directions (CME article)

The treatment metastatic non-small cell lung cancer (NSCLC) is largely influenced by the incorporation of immune checkpoint inhibitors (ICI) in the frontline setting. There are several ICI approved for the management of NSCLC based on the PD-L1 expression of the tumors. PD-L1 is a controversial biomarker with various inconsistencies in expression owing to temporal and spatial heterogeneity. Tumor mutational burden is another much studied biomarker associated with its own challenges and questionable concordance with tumor PD-L1 expression. In this article, we aim to discuss the challenges associated with the existing biomarkers, highlighting the need for emerging biomarkers that can help with decision making in the management of this there where several therapeutic options exist. There are emerging “me too” PD-1/PD-L1 drugs which may serve its purpose in many counties where there is limited access to current approved ICIs. What is increasingly apparent is the need to move the needle forward in the treatment of NSCLC and we will discuss the challenges associated with the current therapeutic landscape and the emerging checkpoints and the future directions that are being explored in the management of metastatic NSCLC.

Single cell transcriptome analysis of MCF-7 reveals consistently and inconsistently expressed gene groups each associated with distinct cellular localization and functions.

Single cell transcriptome (SCT) analysis provides superior resolution to illustrate tumor cell heterogeneity for clinical implications. We characterized four SCTs of MCF-7 using 143 housekeeping genes (HKGs) as control, of which lactate dehydrogenase B (LDHB) expression is silenced. These SCT libraries mapped to 11,423, 11,486, 10,380, and 11,306 RefSeq genes (UCSC), respectively. High consistency in HKG expression levels across all four SCTs, along with transcriptional silencing of LDHB, was observed, suggesting a high sensitivity and reproducibility of the SCT analysis. Cross-library comparison on expression levels by scatter plotting revealed a linear correlation and an 83–94% overlap in transcript isoforms and expressed genes were also observed. To gain insight of transcriptional diversity among the SCTs, expressed genes were split into consistently expressed (CE) (expressed in all SCTs) and inconsistently expressed (IE) (expressed in some but not all SCTs) genes for further characterization, along with the 142 expressed HKGs as a reference. Distinct transcriptional strengths were found among these groups, with averages of 1,612.0, 88.0 and 1.2 FPKM for HKGs, CE and IE, respectively. Comparison between CE and IE groups further indicated that expressions of CE genes vary more significantly than that of IE genes. Gene Ontology analysis indicated that proteins encoded by CE genes are mainly involved in fundamental intracellular activities, while proteins encoded by IE genes are mainly for extracellular activities, especially acting as receptors or ion channels. The diversified gene expressions, especially for those encoded by IE genes, may contribute to cancer drug resistance.

Spectra-first feature analysis in clinical proteomics - A case study in renal cancer.

In proteomics, useful signal may be unobserved or lost due to the lack of confident peptide-spectral matches. Selection of differential spectra, followed by associative peptide/protein mapping may be a complementary strategy for improving sensitivity and comprehensiveness of analysis (spectra-first paradigm). This approach is complementary to the standard approach where functional analysis is performed only on the finalized protein list assembled from identified peptides from the spectra (protein-first paradigm). Based on a case study of renal cancer, we introduce a simple spectra-binning approach, MZ-bin. We demonstrate that differential spectra feature selection using MZ-bin is class-discriminative and can trace relevant proteins via spectra associative mapping. Moreover, proteins identified in this manner are more biologically coherent than those selected directly from the finalized protein list. Analysis of constituent peptides per protein reveals high expression inconsistency, suggesting that the measured protein expressions are in fact, poor approximations of true protein levels. Moreover, analysis at the level of constituent peptides may provide higher resolution insight into the underlying biology: Via MZ-bin, we identified for the first time differential splice forms for the known renal cancer marker MAPT. We conclude that the spectra-first analysis paradigm is a complementary strategy to the traditional protein-first paradigm and can provide deeper level insight.

The motivational self is more than the sum of its goals.

I present evidence in favor of an overarching motivational self: a mental function that regulates expression of multiple goals. Goals often conflict with each other, and the role of a motivational self is to consciously or unconsciously prioritize pursuit of these goals. When observing inconsistency in expression of goals, it is therefore useful to consider whether the motivational self is attempting to balance between conflicting goals or if such inconsistency results from temporary self-control weakness.

Intronic enhancement of angiotensin II type 2 receptor transgene expression in vitro and in vivo

The angiotensin II type 2 receptor (AT2R) can influence a variety of intracellular signaling molecules and cellular functions. However, its physiological functions and the roles of introns in the regulation of its expression have not been well determined. We first demonstrated that both intron 1 and intron 2 of AT2R could significantly enhance AT2R overexpression. Thus, we have provided some new prerequisites for further studies on the mechanisms that control AT2R gene expression. Next, we established a highly efficient method of delivering this receptor in vitro and in vivo using an AT2R recombinant adenoviral vector containing two introns of the AT2R. The vector may be useful in helping to uncover AT2R physiological functions and also for gene therapy related to AT2R. Moreover, we determined the important role of introns in gene expression cassettes and the inconsistency of expression between the targeted gene and the reporter gene.

Terminological investigations into specialized knowledge and texts

Research has traditionally portrayed language for specialized purposes (LSP) as devoid of idiosyncrasy, indeterminacy, context-specificity, inter-cultural variation, inconsistency of expression and acceptation, among other attributes associated with general or non-specialized discourse. This article suggests that attitudes and practices spawned by an uncritical reading of traditional LSP research could indeed constitute a hindrance to the adequate processing of specialized material by translators, terminologists and by ordinary readers or communication parties. The article shows that there is cultural-epistemic relativity in specialized discourses, and that indeterminacy/inconsistency of concepts and terms is present in specialized discourses just as in non-specialized material. These two theses are shown to have implications for the establishment of concept and term equivalence, such as is called for during translating, compilation of terminological resources, or even during reading/comprehension.

Inconsistency in the expression of locomotor and ERG circadian rhythms in the German cockroach, Blattella germanica (L.).

ERG recordings from German cockroaches showed that the amplitude of light-evoked responses have a circadian rhythmicity in adult males that coincided with the locomotor circadian rhythm. The peak of the response occurred during the subjective night, and the circadian period was less than 24 h under DD condition. In contrast, although the locomotor circadian rhythm was masked by the development of ovaries and pregnancy in females, their visual responses displayed circadian rhythmicity. This inconsistency in expression of locomotor and visual sensitivity circadian rhythms in females implied separate pacemakers for these two overt rhythms. After severing the optic nerves, changes in ERG amplitude of the operated cockroaches still displayed a circadian rhythm under DD condition, demonstrating that the visual sensitive pacemaker was located in the eye and independent from the locomotor pacemaker.

Consistency of textual expression in newspaper articles: an argument for semantically based query expansion

This article investigates how consistent different newspapers are in their choice of words when writing about the same news events. News articles on the same news events were taken from three Finnish newspapers and compared in regard to their central concepts and words representing the concepts in the news texts. Consistency figures were calculated for each set of three articles (the total number of sets was sixty). Inconsistency in words and concepts was found between news articles from different newspapers. The mean value of consistency calculated on the basis of words was 65 per cent; this however depended on the article length. For short news wires consistency was 83 per cent while for long articles it was only 47 per cent. At the concept level, consistency was considerably higher, ranging from 92 per cent to 97 per cent between short and long articles. The articles also represented three categories of topic (event, process and opinion). Statistically significant differences in consistency were found in regard to length but not in regard to the categories of topic. We argue that the expression inconsistency is a clear sign of a retrieval problem and that query expansion based on semantic relationships can significantly improve retrieval performance on free‐text sources.