Inclusion Of Fat Research Articles

Suckling and Excess Fat in the Postpartum Reproductive Activity of Hair Sheep

Objective: To evaluate the effect of excess fat and suckling control on postpartum reproductive activity of hair ewes. Design/Methodology/Approach: Eighty-one (81) hair ewes with average weight of 58±8 kg were used, which on postpartum day 7 were assigned to one of four treatments under a completely randomized design with 2×2 factorial arrangement. Factor A is the type of suckling [continuous suckling (CS) and suckling control (SC)] and factor B, excess fat (with or without). In CS, ewes remained with their offspring 24 hours a day (n=16). In continuous suckling plus fat (CSF), ewes remained with the offspring all day plus the addition of 8% in dietary fat (n=24). In SC, it was 30 min of suckling only twice per day (n=14). In suckling control plus fat (SCF), controlled suckling lasted 30 min, twice a day plus 8% fat (n=25). Results: The response to the treatments was similar (p > 0.05) in onset of estrus, return rate, gestation, prolificacy and fertilization; for the lambing rate variable, there were differences (p ≤ 0.05), the SCF treatment was higher (68%) compared to CS (50%) and CSF (50%), but equal (p < 0.05) to SC (57.1%). Implications: In this experiment, it was observed that the SCF treatment presented a better response, implying that controlling suckling and providing excess fat helps to restore ovarian activity sooner after lambing. Conclusions: Suckling control plus inclusion of dietary excess fat in ovulation induction protocols during early postpartum (around day 25 postpartum) can be included in routine reproductive management of flocks, improving the reproductive efficiency of hair sheep.

Nutritional management of dairy animals for sustained production under heat stress scenario

Dairy industry plays a significant role in the agriculture sector for sustainable growth. Heat stress, on the other hand, has been proven to have a detrimental impact on dairy output in terms of growth, reproductive performance and milk production in dairy animals, especially in tropical areas. Heat stress is one of the most significant issues facing the dairy industry, as rising temperatures and humidity limit animal productivity throughout the summer, resulting in devastating economic repercussions. The purpose of this review is to gather knowledge on the effects of heat stress on dairy output and how to ameliorate them. The diversion of energy resources from the production to the adaptation pathway may be responsible for the loss in productive capacity of dairy animals, when they are exposed to heat stress. There are different approaches pertaining to relieving the adverse effects of heat stress on dairy production system. These approaches may be classified into three major categories viz. genetic, management and nutritional interventions. These approaches might help dairy animals to perform better by reducing the harmful impacts of heat stress. Appropriate shelter design, giving shade, employing sprinklers, installing cooling devices, and using fans and ventilation systems are among the management strategies. The nutritional interventions comprise ration balancing and providing essential micronutrients to improve the productive and reproductive performance. Some of the most widely used dietary measures to ensure optimal production are inclusion of protein or fat (prill fat), micronutrients antioxidants (vitamins and minerals) and some feed additives (Astaxanthin, betaine, melatonin, Chlorophytum borivilianum) in the diet. These antioxidants and feed additives can be used to attenuate the negative effects of environmental stress. Furthermore, providing adequate energy and antioxidants help to ensure optimum growth, milk production and reproduction efficiency during heat stress. This review provides an overview of the consequences of heat stress on dairy animals, emphasizes essential nutritional strategies for heat stress reduction in dairy animals, and evaluates the influence of various feed supplements on growth, productivity and physiology.

When Two plus Two Is More than Four: Evidence for a Synergistic Effect of Fatty Acids on Peroxisome Proliferator-Activated Receptor Activity in a Bovine Hepatic Model.

The inclusion of fat in livestock diets represents a valuable and cost-effective way to increase the animal’s caloric intake. Beyond their caloric value, fatty acids can be understood in terms of their bioactivity, via the modulation of the ligand-dependent nuclear peroxisome proliferator-activated receptors (PPAR). Isotypes of PPAR regulate important metabolic processes in both monogastric and ruminant animals, including the metabolism of fatty acids (FA), the production of milk fat, and the immune response; however, information on the modulation of bovine PPAR by fatty acids is limited. The objective of this study was to expand our understanding on modulation of bovine PPAR by FA, both when used individually and in combination, in an immortalized cell culture model of bovine liver. Of the 10 FA included in the study, the greatest activation of the PPAR reporter was detected with saturated FA C12:0, C16:0, and C18:0, as well as phytanic acid, and the unsaturated FA C16:1 and C18:1. When supplemented in mixtures of 2 FA, the most effective combination was C12:0 + C16:0, while in mixtures of 3 FA, the greatest activation was caused by combinations of C12:0 with C16:0 and either C18:0, C16:1, or C18:1. Some mixtures display a synergistic effect that leads to PPAR activation greater than the sum of their parts, which may be explained by structural dynamics within the PPAR ligand-binding pocket. Our results provide fundamental information for the development of tailored dietary plans that focus on the use of FA mixtures for nutrigenomic purposes.

Mitigation of Enteric Methane Emission through Feed Modification and Rumen Manipulation

<p class="awabstrak2">The major of gas emission in the livestock sector are in the form of methane produced by microbial activity in the rumen. The emission of methane cause global warming and is predicted to keep increasing. Feed modification and rumen manipulation are important ways that can be used to mitigate methane emission. Based on this condition, this paper aims to describe several ways to mitigate methane emission using feed and rumen modification for smallholder farmers. Feed modification can be done using high Non-Fiber Carbohydrate (NFC) content in feed and also using balance nutrient feed. Meanwhile, rumen modification can be done through inlcusion of feed additive, microbial products, and oils. Providing feed contains high NFC as much as 21.8-53%DM would decrease methane emission by 3.03-28.33%. While providing feed contains balance nutrients would potentially decrease 21.87% of methane emission. Feed additive addition as much as 0.0011-12%DM decreased 0.59-78% of methane emission. Bacterial inclusion as much as 0.7x10<sup>8</sup> – 3,6x10<sup>11</sup>CFU decreased 0- 18.57% of methane emission. Oil or fat inclusion as much as 6%DM decreased 6.02-24.53% of methane emission. A combination of methods can be used to optimize methane mitigation and it can be applicable for farmers to raise their livestock in friendly environment.</p>

Protected or Unprotected Fat Addition for Feedlot Lambs: Feeding Behavior, Carcass Traits, and Meat Quality

Simple SummaryThe use of lipids in ruminant diets aims to increase energy density without affecting the animal’s performance; however, its use can be toxic to the ruminal microbiota, which can be avoided with the use of protected fats. Diets with the inclusion of different fat sources (whole soybean grain, corn germ, calcium salt of fatty acids, and soybean oil) were tested to evaluate the effects of unprotected or protected fats on feeding behavior, carcass characteristics, and quality of the meat of feedlot lambs. The use of calcium salts from fatty acids in feedlot lambs’ diets improves the quantitative and qualitative characteristics of the carcass and meat.This study aimed to evaluate the effect of the inclusion of protected or unprotected fats in the diet of feedlot lambs on feeding behavior, productive characteristics, carcass traits, and meat quality. Forty male Dorper × Santa Inês lambs (22.27 ± 2.79 kg) were randomly assigned to treatments in a completely randomized design. The experimental treatments consisted of five diets: no added fat (NAF), whole soybeans (WSB), calcium salts of fatty acids (CSFA), soybean oil (SO), and corn germ (CG). The total intake of dry matter (DMI) (p < 0.001) and neutral detergent fiber (NDFI) (p = 0.010) were higher in the CSFA and NAF diets. Feeding behavior, morphometric measurements, physicochemical characteristics, and centesimal composition of the Longissimus lumborum muscle were similar between treatments (p > 0.05). The CSFA diet provided higher production (p < 0.05) and better-quality carcasses. The inclusion of fat sources increased the concentration of polyunsaturated fatty acids (p < 0.05). The use of calcium salts of fatty acids in feedlot lambs’ diets provides better quantitative and qualitative characteristics of the meat and carcass.

Centrifugation does not remove bacteria from the fat fraction of human milk

Analysis of the human milk microbiome is complicated by the presence of a variable quantity of fat. The fat fraction of human milk is typically discarded prior to analysis. It is assumed that all cells are pelleted out of human milk by high speed centrifugation; however, studies of bovine milk have reported that bacteria may remain trapped within the fat fraction. Here, the bacterial DNA profiles of the fat fraction and cell pellet of human milk (n = 10) were analysed. Human and bacterial DNA was consistently recovered from the fat fraction of human milk (average of 12.4% and 32.7%, respectively). Two low-abundance Staphylococcus species (< 0.5% relative abundance) was significantly more abundant in the cell pellet compared to the fat fraction (P < 0.04), and three low-abundance species (< 5% relative abundance) were recovered from one fraction only. However, inclusion of fat reduced the efficiency of DNA extraction by 39%. Culture-based methods were used to quantify the distribution of an exogenously added strain of Staphylococcus aureus in human milk fractions. S. aureus was consistently recovered from the fat fraction (average 28.9%). Bacterial DNA profiles generated from skim milk or cell pellets are not representative of the entire human milk microbiome. These data have critical implications for the design of future work in this field.

CHEMICAL CHARASTERISTIC OF RABBIT HIBRIDS

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The aim and purpose of the analysis was the influence of the gender m/f of the chemical, microbiological and sensory composition. Flemish giant rabbit and New Zealand white rabbits (group ON), were used as a material for the study. Rabbits were fed ad libitum with commercial pelleted feed, with the addition of small amounts of green feed and hay. The experiment included 6 rabbits, 3 males and 3 females. Previously defined live weight of 1800 to 2500 g was achieved within 77 days and after that they were slaughtered. This paper provides the conducted results of the research in regard of the chemical, microbiological and sensory composition of the rabbit meat. &nbsp;&nbsp;&nbsp;&nbsp; The average composition (n-6) of the inclusion of the water in the thigh, shoulder blade and the back is 71.89% / 74.51% / 72.61%, the inclusion of proteins is 19.46% / 18.62% / 21.28%, the inclusion of fats is 3.34% / 4.36% / 2.46%, the inclusion of minerals is 1.16% / 1.10% / 1.19%. In meat parts, are not found bacteria of the following types: Clostridium, Staphylococus, Proteus, Escherichia. The average microbiological content (24 hours post mortem) of Total bacteria (baccilus) number varies from 2.44 to 2. 58 (log CFU/g). Male rabbits are tougher, with more intensive color, taste and smell.

Assessment of the quality and safety of the use of vegetable fats in meat production

The high demand for quality and nutritious foods is constantly growing. To provide the population with high-quality meat products, it is necessary to determine the impact of the components introduced into the feed ration, which have a negative and positive impact on the production of these products, and then develop and apply tools to improve their quality. The oil and fat industry is a potential source of production of high-quality feed products for various types of animal husbandry, since the composition of oil-containing raw materials contains all the necessary components for animal nutrition. The main objective of our study was to assess and predict the quality and safety of the use of vegetable fats (palm, sunflower, soybean, rapeseed and flaxseed oils) in the production of beef meat. It has been found that the additional inclusion of vegetable fats enhances the intensity of metabolic processes in the body and thereby contributes to an increase in meat productivity.

Effects of dietary fat source and green tea (Camellia sinensis) extract on genes associated with lipid metabolism and inflammatory responses in female broiler chickens

This study was aimed to investigate the effects of dietary fat sources and green tea extract (GTE) on gene expression associated with lipid metabolism and inflammation in broiler chicken. A total of 300 female Ross 308 broiler chicks were allocated to six dietary treatments in a completely randomised design with a factorial arrangement of two levels of GTE (0 and 500 mg/kg diet) × three levels of fat inclusion [without fat (control group), soybean oil (SO) and tallow (Ta)]. Liver fat (LF), liver methallothionin (LMT) content, and lipoprotein lipase (LPL), adipose triglyceride lipase (ATGL), tumour necrosis factor α (TNF-α), and interleukin (IL-6)] genes expression were investigated. Relative expression and statistical analyses of genes expression were assessed using REST software. The effect of added dietary fats was significant and improved performance parameters compared to the control group (p < .05). The highest abdominal fat and blood triglyceride, and the lowest carcase yield, were achieved in chicks fed SO-supplemented diet (p < .05). Chicks fed a diet supplemented with SO showed an overexpression and a decrease of LPL and ATGL compared to the Ta-supplemented and control groups, respectively (p < .05). GTE supplementation was effective on LPL and ATGL expression and ameliorated the effect of SO on abdominal fat percentage. TNF-α expression, LMT content, and relative weight of lymphoid organs were affected neither by GTE nor by the fat sources. There was no detection of IL-6 gene expression in adipose tissue. The results of this study demonstrated that diets rich in SO were capable increase fat deposition by an increase and decrease in LPL and ATGL gene expression, respectively. However inclusion of GTE in broiler diets alleviated the LPL-increasing and ATGL-reducing effects of SO and ameliorated the effect of SO on abdominal fat mass. Highlights Diets supplemented with SO increased abdominal fat mass by changes in LPL and ATGL gene expression. Obesity was not effective in the expression of inflammatory genes (TNF-α and IL-6), indicating a lack of connection between inflammation and obesity in broiler chicken. GTE (500 mg/kg diet) addition to the diets supplemented with SO ameliorated the effect of SO on LPL and ATGL gene expression and abdominal fat mass.

Inclusión de grasa en dieta de cerdas primíparas: efecto sobre la reproducción y performance de la camada

&lt;p&gt;La incorporación de grasa en la dieta podría mejorar la performance reproductiva&lt;br /&gt;de cerdos en producción, principalmente durante las épocas más calurosas. Para comprobar&lt;br /&gt;esta hipótesis se evaluó el efecto de la inclusión de grasa a diferentes niveles sobre&lt;br /&gt;variables reproductivas de cerdas primíparas en condiciones de subtrópico. Se utilizaron 3&lt;br /&gt;grupos de 6 animales (n=18) a los cuales se les suministraron 3 dietas con diferentes niveles&lt;br /&gt;de inclusión de grasa (0%, 3,5% y 7%) desde el día 90 de gestación y durante la lactación&lt;br /&gt;(21 días). Las variables medidas fueron el número de lechones nacidos totales, nacidos vivos,&lt;br /&gt;muertos y peso de la camada al nacimiento y al destete, mientras que sobre las hembras se&lt;br /&gt;registró el intervalo de retorno al celo. Se empleó un diseño completamente aleatorizado&lt;br /&gt;y los datos fueron analizados mediante ANOVA con el software estadístico Infostat®. No&lt;br /&gt;se encontraron diferencias al incorporar grasa, en relación al número de lechones nacidos&lt;br /&gt;vivos, muertos, totales, peso al nacimiento e intervalo-destete celo (p&amp;gt;0,05) mientras que el&lt;br /&gt;número y peso de lechones al destete se vio influenciado de manera significativa (p&amp;lt;0,05).&lt;br /&gt;En conclusión, la adición de grasa en dietas de cerdas primíparas no afecta variables al nacimiento ni el intervalo detete-celo, pero mejora el número y peso de los lechones al destete.&lt;/p&gt;

Metabolic and performance responses to the replacement of lactose by fat in milk replacer formulations for dairy calves

The recent trend in the dairy industry towards elevated planes of milk feeding of young calves requires reconsideration of calf milk replacer (CMR) formulations. The fat:lactose ratio in CMR is typically lower than that of whole milk and effects of increasing fat inclusion at the expense of lactose in CMR on nutrient metabolism and gut function of rearing calves are not sufficiently understood. Therefore, the current study aimed to determine the effect of increasing replacement of lactose by fat on growth performance, nutrient digestibility and metabolism, and glucose/insulin sensitivity. A total of 40 male calves (1.7 ± 0.10 days of age, 46.7 ± 0.76 kg BW) were blocked based on arrival date and randomly assigned to one of four treatments containing differing levels of fat and lactose (18F: 18.8% and 47.6%; 22F: 22.3% and 42.8%; 26F: 26.0% and 38.6%; 30F: 30.1% and 33.8%, fat and lactose DM, respectively). Calves were individually housed for the duration of the 11 week study and received their CMR (150 g/L) twice a day (0700 and 1600 h) from a teat bucket. The CMR feeding schedule consisted of 6 L/d from d 2 to 14, 7 L/d from d 15 to 56, and then 4 L/d during gradual weaning from 56 to 63 days. Pelleted starter, chopped straw, and water were available ad libitum throughout the study. Measurements included feed intake, growth, nutrient digestibility, fecal composition, and blood parameters. A glucose tolerance test (GTT) was performed between 28 and 32 days of age. By design, metabolizable energy intake from CMR increased linearly with fat level, but this did not result in a difference in BW, ADG, or concentrate intake. Fecal composition remained unaffected by treatment except for higher fat content in 22F compared to 26F. Also, plasma non-esterified fatty acids and total bilirubin differences were limited to 22F having the highest values whereas 26F had the lowest values. Regarding the GTT, total area under the curve (AUC) for glucose was highest in 22F and 26F and lowest in 18F whereas the AUC between 30 and 60 min for glucose was highest in 26F and lowest in 18F and 22F. Overall, altering the lactose:fat ratio in CMR did not affect growth performance while having minor effects on nutrient metabolism, but future investigation should focus on physiological consequences and effects in adult life to understand biological impact of fat and lactose levels in CMR for rearing calves.

Physicochemical and Sensorial Evaluation of Meat Analogues Produced from Dry-Fractionated Pea and Oat Proteins.

Pea protein dry-fractionated (PDF), pea protein isolated (PIs), soy protein isolated (SIs) and oat protein (OP) were combined in four mixes (PDF_OP, PIs_OP, PDF_PIs_OP, SIs_OP) and extruded to produce meat analogues. The ingredients strongly influenced the process conditions and the use of PDF required higher specific mechanical energy and screw speed to create fibrous texture compared to PIs and SIs. PDF can be conveniently used to produce meat analogues with a protein content of 55 g 100 g−1, which is exploitable in meat-alternatives formulation. PDF-based meat analogues showed lower hardness (13.55–18.33 N) than those produced from PIs and SIs (nearly 27 N), probably due to a more porous structure given by the natural presence of carbohydrates in the dry-fractionated ingredient. PDF_OP and PIs_PDF_OP showed a significantly lower water absorption capacity than PIs OP and SIs_OP, whereas pea-based extrudates showed high oil absorption capacity, which could be convenient to facilitate the inclusion of oil and fat in the final formulation. The sensory evaluation highlighted an intense odor and taste profile of PDF_OP, whereas the extrudates produced by protein isolates had more neutral sensory characteristics. Overall, the use of dry-fractionated protein supports the strategies to efficiently produce clean-labeled and sustainable plant-based meat analogues.

Animal fat and vitamin E in rabbit diets: Total tract apparent digestibility, growth performance, carcass and meat quality traits

The present study tested the effect of a dietary inclusion with vitamin E and pork lard on the apparent digestibility of the nutrients, the growth performance, the carcass traits, the physical meat quality, and the α-tocopherol content. A total of 60 hybrid rabbits were reared in individual cages from weaning (35 days of age) until slaughter (78 days of age). A control diet with no supplements, one diet supplemented with 2% pork lard, and two diets that used the aforementioned diets supplemented with an additional 200 mg/kg α-tocopheryl acetate were designed. The diets were isoprotein and isoenergy. The fat inclusion increased the crude protein (P &amp;lt; 0.05) and ether extract (P &amp;lt; 0.001) total tract apparent digestibility, and the same was observed for the vitamin E inclusion (P &amp;lt; 0.001 for both variables). This improved the dietary digestible protein content (P &amp;lt; 0.05), which increased the digestible protein to digestible energy ratio (P &amp;lt; 0.001). The fat × vitamin E interaction was observed for the total tract apparent digestibility of the ether extract (P &amp;lt; 0.001), the neutral detergent fibre (P &amp;lt; 0.05) and the acid detergent fibre (P &amp;lt; 0.01). The growth traits were unaffected, with the exception of the feed conversion ratio that improved with the vitamin E addition (P &amp;lt; 0.05). Similarly, the carcass traits remained unaffected, with the exception of the perirenal and total fat incidence that increased with the fat supplement (P &amp;lt; 0.05), and the scapular fat that was reduced with the vitamin E inclusion (P &amp;lt; 0.05). The meat L* (lightness), a* (redness), b* (yellowness) colour values and ultimate pH were unaffected by the experimental treatments, even though a fat × vitamin E interaction was observed for the a* and chroma values of the Longissimus thoracis et lumborum muscle (P &amp;lt; 0.05). Both the fat (P &amp;lt; 0.05) and vitamin E (P &amp;lt; 0.001) dietary inclusion increased the meat α-tocopherol content. Based on the results, it was concluded that the 2% dietary inclusion of animal fat did not provide more benefits for the considered parameters than the sole α-tocopheryl acetate incorporation, but contributed to the increase in the vitamin E content in the meats.

Animal Fats in Rabbit Feeding – A Review

Abstract The purpose of this article is to overview the history of feeding rabbits with different types of animal fats, and to discuss their effects on rabbit performance and quality of their products. Other aspects of the inclusion of various animal fats in rabbit diets are also described. This article is based on the analysis of relevant scientific literature and presents animal fats fed to rabbits, such as beef tallow, butter, pork lard, poultry fat, fish oil, krill oil, oil extracted from insect larvae, mixtures of various animal fats, and mixtures of animal and vegetable fats. The reported papers describe the effect of fats on growth performance, lactation, rearing performance, meat quality, and health status of rabbits. It is notable that in many cases, various animal fats were often an integral part of numerous diets or were included in control diets. The presented information demonstrates that animal fat can be fed to rabbits at 2–4% of the diet without negative effects on reproductive performance, growth performance and quality of meat obtained. Rabbits were used as model animals in many studies in which fat was added to balance the diets and to increase their energy value, especially when investigating various cardiovascular and obesity-related diseases.

In vitro embryo production in Nelore cows supplemented with calcium salts of long-chain fatty acid

This study was performed to evaluate the effects of supplementation of lactating Nelore cows with calcium salts of long-chain fatty acids (Ca-LCFA) of soybean oil on in vitro oocytes competency, cleavage rate, embryo production rate, pregnancy rate, and sex ratio of transferred embryos. Fifteen multiparous Nelore cows grazed on a Tifton 85 pasture were allotted to two treatments: (1) supplement without fat inclusion (CONT, n = 6) and (2) supplement containing 47.2 g Ca-LCFA /kg (to provide an intake of 0.2 kg Ca-LCFA/day) based on dry matter (n = 9). Animals were subjected to 2.53 ± 1.35 rounds of consecutive ovum pick up at intervals of 27.65 ± 21.72 days. Oocytes were morphologically evaluated, selected, maturated, fertilized, and cultivated for embryo production in vitro and then transferred into recipients who had previously undergone an embryo transfer protocol at a fixed time. The number of aspirated oocytes, the proportion of viable oocytes, cleavage rate, embryo percentage, pregnancy rate, and sex ratio were not affected when Nelore cows were supplemented with Ca-LCFA of soybean oil compared with non-supplemented Nelore cows. In conclusion, our results suggest that feeding a supplement containing 47.2 g Ca-LCFA/kg (to provide an intake of 0.2 kg Ca-LCFA/day) to Nelore cows does not affect in vitro embryo production in lactating Nelore cows. However, further studies using higher dietary doses of fat could be useful to determine the potential of fat supplementation to improve in vitro embryo production.

Growth, carcass and adipose tissue characteristics of dairy origin bulls offered concentrate rations of increasing energy density

The hypothesis that inclusion of a rumen-inert fat in the diet of dairy-origin bulls would enhance EUROP carcass fat classification and tissue fatty acid composition was tested. Sixty bulls (age = 508 days, s.d. 29.1) were offered ad libitum, a barley-based concentrate ration that contained 0, (control) 26 (MEGA1) or 81 g (MEGA2) calcium salts of palm oil fatty acids (Megalac)/kg such that the rations contained 25, 49 and 116 g acid-hydrolysed ether extract /kg dry matter (DM), respectively. Intake (per pen of 5 bulls), live weight and ultrasonic backfat thickness were measured during a 130 day feeding period after which bulls were slaughtered, carcass characteristics recorded and a sample of longissimus muscle (LT) and subcutaneous adipose tissue (SAT) collected for fatty acid analysis. Subcutaneous fat depth was similar for control and MEGA1 bulls but lower (P < 0.05) for MEGA2 bulls after 60 days of the study. After 125 days, compared to control bulls subcutaneous fat depth was higher for MEGA1 bulls but lower for MEGA 2 bulls (P < 0.05). Carcass gain tended (P = 0.074) to be lower for MEGA2 bulls compared to control and MEGA1 bulls, which reflected their lower consumption of concentrate DM and metabolisable energy (ME) but the efficiency with which consumed DM or ME was converted to live or carcass weight did not differ significantly between rations. There was no difference between control and MEGA1 bulls for any measurement of carcass fatness. Compared to control and MEGA1 bulls, carcasses from MEGA2 bulls were lighter, had a lower depth of fat covering the LT muscle measured post-mortem (P < 0.05) and a numerically lower carcass fat classification. There were only minor effects of ration composition on the fatty acid profile of LT or SAT. With regard to nutritional indices in meat, compared to control bulls, inclusion of Megalac did not affect the polyunsaturated (P) to saturated fatty acid ratio or the n-6 to n-3 P ratio of tissue lipids. It is concluded that for the type of animal and feeding arrangement in this study, rumen inert fat inclusion was not an effective strategy to enhance carcass fat classification or the nutritional value of beef.

Fat Inclusion Level, NaCl Content and LAB Starter Cultures in the Manufacturing of Italian-Type Ostrich Salami: Weight Loss and Nutritional Traits

The experiment studied the effect of two different fat inclusion levels (30% and 40%), NaCl contents (2.4 and 2.6%) and starter cultures (lactic acid bacteria (LAB) 6: L. curvatus/S. xylosus; LAB 8: L. sakei/S. xylosus) on the weight loss and nutritional composition of Italian-type ostrich salami. With this purpose, 8 batches of 9 salami each (n = 72) were prepared. Salami were ripened for 20 weeks: weight loss was monitored throughout the experiment, while salami nutritional composition was evaluated at 10 and 20 weeks of ripening. The lowest fat and highest salt inclusion levels provided the highest cumulative weight loss throughout the trial. At 10 weeks of ripening, salami with 40% fat were the richest in moisture and fat, whereas the leanest ones had the highest protein, ash and cholesterol contents. LAB 6 provided salami with the highest moisture and protein, while LAB 8 increased fat and cholesterol contents. At 20 weeks of ripening the proximate composition of ostrich salami was solely affected by fat inclusion level, with similar findings to those observed at 10 weeks. Overall, fat inclusion level had a great impact on the weight loss and nutritional composition of Italian-style ostrich salami. Reducing the NaCl inclusion from 2.6% to 2.4%, the weight loss of ostrich salami was retarded by approximately 1 week, without affecting the nutritional composition of the final product. Results of the study suggested that it is feasible to produce salami with lower fat and salt contents, while ensuring satisfactory product quality.

Effects of various fat delivery methods in starter diet on growth performance, nutrients digestibility and blood metabolites of Holstein dairy calves

The effects of different fat inclusion methods in starter feed on performance, growth, health indices, digestibility, and blood metabolites were evaluated in Holstein dairy calves. Forty-four 3-day-old Holstein dairy calves (averaging 39 kg BW) were allocated to treatments in a completely randomized block design (11 calves per treatment; 6 females and 5 males) as follows: (1) no fat or oil supplementation to starter feed (CTR); (2) dietary starter mixed with soy oil (DMSO); (3) fat included in the form of extruded soybean seed (ESBS); and (4) fat included in the form of roasted soybean seed (RSBS). The experimental starter diets were maintained with iso-energetic, iso-nitrogenous, and ruminal iso-degradable proteins. Dietary fat content was constant in fat supplemented treatments. All calves were weaned on d 59 of age and maintained in the study until d 73 for final data records. There was no difference in starter intake among experimental treatments until d 47; however, it was reduced in the transition period as well as in the post-weaning period in fat supplemented diets compared with the CTR treatment. No changes were found for BW among treatments in either the pre- or post-weaning period. Feed efficiency was relatively constant among treatments. Regardless of the dietary fat inclusion method, wither height was lower in all calves fed a diet supplemented with fat compared with the CTR treatment (p = 0.04). The fat-supplemented calves tended to have higher fecal score than the un-supplemented ones (p = 0.08) and showed lower fecal consistency in these treatments. Blood albumin was greatest (p = 0.04) and blood cholesterol was lowest (p = 0.05) in the CTR treatment compared to the supplemented treatments. No differences were observed for other blood metabolites, liver enzymes, or insulin concentrations among treatments. Under the conditions of this experiment, it could be concluded that fat supplementation in the form of processed oilseeds rather than direct mixing of oil with the starter diet resulted in better fecal scores and fiber digestibility in dairy calves; however, fat inclusion per se is not advisable in the starter diet of dairy calves.

Obesity, insulin resistance, adiponectin, and PPAR-γ gene expression in broiler chicks fed diets supplemented with fat and green tea (Camellia sinensis) extract

Adipose tissue is an active endocrine organ secreting several adipokines, especially adiponectin, that play an important role in regulating insulin function in the body of mammals. Therefore, this study was aimed to investigate the association between abdominal fat deposit, insulin resistance, peroxisome proliferator-activated receptor gamma (PPAR-γ), and adiponectin gene (AG) expression in broiler chicks fed diets high in unsaturated fat supplemented with green tea extract (GTE). A total of 300 one-day-old female Ross 308 broiler chicks were allocated to 6 dietary treatments in a completely randomized design with a factorial arrangement of two levels of GTE (0 and 500 mg/kg diet) × three levels of fat inclusion [without fat (control group), soybean oil (SO), and tallow (Ta)]. Each treatment was replicated five times. At the end of the experiment (day 49), two chicks from each replicate weighing an average of pen weight were bled and then slaughtered for further analysis. Abdominal fat percentage, fasting concentration of blood glucose, triglyceride and insulin, glycogen reserves of breast and liver tissues, and PPAR-γ and AG expression were determined. The insulin resistance index of the Quantitative Insulin Sensitivity Check Index (QUICKI) was calculated using the fasting plasma glucose and insulin concentrations. The highest abdominal fat percentage and the lowest carcass yield were obtained in chicks fed SO-supplemented diet (P < 0.05). Chicks fed diet supplemented with SO showed the highest PPAR-γ gene expression (P < 0.05). SO-rich diets suppressed AG expression in chickens' abdominal fat tissue, and the birds fed with SO-supplemented diet showed a significant decrease in AG expression compared with the control (P < 0.05). Chicks fed diet supplemented with SO showed lower QUICKI and breast glycogen reserve compared with the control group (P < 0.05). A significant increase in blood glucose and triglyceride concentrations was observed in birds fed SO-supplemented diets (P < 0.05). AG and PPAR-γ expression increased and decreased by GTE, respectively. QUICKI tended (P = 0.09) to be greater in GTE-supplemented chicks; however, the effect of GTE supplementation on carcass yield, abdominal fat percentage, and blood insulin and glucose concentration was not significant. The findings of this study showed that SO-rich diets via increased PPAR-γ gene expression and decreased AG expression in abdominal fat may lead to insulin resistance in female broiler chicks.

277 Effects of cow supplementation on beef quality and expression of lipogenic and fibrogenic genes in the muscle of progenies fed diets with or without high inclusion of rumen-protected fat

Abstract The objective was to evaluate the effects of Nellore cows supplementation, during mid to late gestation, on beef quality and expression of genes involved in the deposition of fat and collagen in the muscle of progenies fed diets with or without high inclusion of rumen-protected fat. Forty Nellore steers with an initial average body weight of 340 + 9.38 kg were distributed in a completely randomized design, and arranged in a 2 × 2 factorial design. Therefore, the following treatments were assessed: nutritional management of cows without supplementation (NSUPP); or with supplementation (SUPP: 330 g CP and 2.11 Mcal/cow/day); and progeny feedlot diets without rumen-protected fat (NRPFAT) or with rumen-protected fat (RPFAT: 6% calcium salts). Samples of longissimus thoracis muscle were used for meat quality analyses: pH, color, chemical composition and 14 days aging shear force; and for gene expression analysis using the RT-qPCR. Statistical analyses were done using the GLM procedure of SAS. The maternal supplementation and the use of RPFAT in feedlot diets did not affect muscle pH (5.7; SEM +0.029), L* (40.0; SEM +1.231), a* (11.9; SEM +0.876), fat content (2.3%; SEM +0.306), collagen (1.43 mg/g; SEM +0.08), and shear force (4.2 + 0.322 kgf). However, cows’ supplementation increased the expression of CPT2 and tended to up-regulate FASN and ACACA expression (Table 1). In addition, RPFAT increased expression of COL3A, FN1, IGFR1, and ACACA. There was no interaction effect of cow supplementation on gene expression. These results suggest that maternal supplementation may increase the expression of genes related to lipid synthesis and degradation (lipid turnover). On the other hand, the use of high amount of calcium salts increases the expression of genes involved in collagen synthesis but with no effect on collagen content and tenderness. Funded by Trow Nutrition, Fapemig, INCT-Ciência Animal and Capes.