Recent availability of and interest in distillers dried grains with solubles (DDGS) has resulted from the increased production of ethanol. A study was designed to evaluate second-cycle Bovans White laying hens that were fed varying levels of DDGS. Layer performance, egg characteristics, and consumer acceptability served as evaluation criteria. Hens were fed a commercial diet formulated to contain 0, 8, 16, 24, or 32% DDGS for a period of 15 wk. The varying levels of DDGS did not have adverse effects on any of the parameters measured. The 16% DDGS treatment resulted in significantly higher egg production than the 0, 8, and 24% treatments, whereas the 32% treatment was intermediate and was not significantly different from any other treatment. Inclusion of DDGS in the diet led to a significantly darker (L*) and redder (a*) yolk, with a tendency to increase Haugh unit values (P = 0.03), indicating potentially increased interior egg quality. During taste panels, consumers slightly preferred the eggs derived from DDGS-fed hens over eggs that were obtained from hens fed no DDGS in both the flavor acceptability (P = 0.04) and overall acceptability (P = 0.02) categories, but they liked eggs from all treatments. Based on the results from the present study, DDGS could be included at a rate of up to one-third of a commercial layer diet without any significant detrimental effects on the production or egg characteristics of second-cycle hens.