Abstract ‘Chandler’ strawberry plants (Fragaria X ananassa Duch.) were greenhouse grown under natural lighting and then placed into growth chambers at two constant temperatures of 16 °C and 26 °C and two day lengths of 9 h (SD) and 9 h photoperiod (NI) which was night interrupted with 3 h of incandescent radiation at 30-45 μmol s ∼ 1m ∼ 2 PAR. Plants were given different numbers of inductive cycles in growth chambers and then moved to the greenhouse where flowering and growth were monitored. Flowering was completely inhibited at 26 °C, regardless of pretreatment growing conditions such as pot sizes and plant ages, photoperiod and inductive cycles. At 16 °C, SD promoted floral induction compared to NI under all inductive cycles except a 7-day induction. The minimum number of inductive cycles required at 16 °C for floral induction was dependent on photoperiod and prior greenhouse treatment. Flowering rate was also affected by greenhouse treatment, photoperiod and inductive cycles. Runner production was affe...