The aim of this paper is to perform experimental modal analysis of two-storey reinforced concrete frame which designed using BS 8110 without seismic load and validate the experimental result with model using finite element analysis software. Experimental Modal Analysis (EMA) is the process of determining the modal parameters such as natural frequencies, damping factors, modal vectors and mode shape of a structure from a set of frequency response functions. The experimental work was conducted on half-scale of two-storey reinforced concrete frame at Heavy Structural Laboratory. Moreover, the Impact Modal Testing and response accelerometer are employed to determine the modes of vibration of a structure during experiments without imposed any in-plane lateral force. The experimental natural frequencies, which are extracted from measurements, exhibit good agreement with analytical model predictions. It shows that experimental modal analysis method can be usefully employed to investigate the dynamic behaviour of reinforced concrete frame due to the minimum percentage error between experimental modal analysis and computer software analysis.