AbstractPurpose To present results of COMET technique in corneal surface reconstruction in bilateral limbal stem cell deficiency.Methods Study group: 13 patients (17 eyes) suffering from limbal stem cell deficiency (LSCD) after chemical burns (7 eyes) or from aniridia related LSCD (10 eyes). Preoperative vision was ranged from hand movements to 0,05. Oral mucosa epithelium was collected and tripsinized. Single cells were seeded on denuded amniotic membrane. Cultures were carried in standard conditions in supplemented DMEM HAM/F12 mixture medium in presence of inactivated 3T3 fibroblasts for 8 days. Histologic examination and immunostaining for epithelial cytokeratins confirmed epithelial origin before transplantation. After superficial keratectomy carries with stratified epithelium were transplanted on the denuded corneas. Corneal surface stability was evaluated as well as visual outcomes.Results Postoperative follow‐up ranged from 6 to 12 months. 6 months after surgery 76,4 % of eyes showed stabile epithelium. In 23,5 % of eyes corneas reminded cloudy due to recurrent conjuctival neovascularization or stromal haze. All failed grafts were from ocular burns patients group. Visual acuity ranged from hand movement to 0.4.Conclusion COMET is a new method for bilateral corneal epithelial disorders offering the patient satisfactory stabilization of the corneal surface.