Illumination Protocol Research Articles

In vitro photoinactivation of Pseudomonas aeruginosa and Staphylococcus aureus biofilm by a novel multi-dose LED-based illumination method

Significancerecently, an aerosolized light source has been proposed to photo-inactivate pathogens responsible for multidrug-resistant chronic lung infections. To maximize the light source in vivo photokilling efficacy, its emission spectrum was predicted by a semi-theoretical model. To confirm and upgrade the model with experiments, biofilm photoinactivation studies were performed. Approachin vitro biofilms of P. aeruginosa and S. aureus (reference and clinical strains) were photo-inactivated by LED sources at 415, 445, 525 and 623nm. Non-uniform illumination protocol was employed to deliver different doses (10 to 110 J/cm2) in a single experiment. Photokilling efficacy was quantified by CFU counting. Results415nm-peaked light was associated with the maximum photokilling efficacy for all the considered strains. Other wavelengths have a minor or scant effect. Conclusionsbiofilm photoinactivation was studied as a function of both dose and illumination wavelength. Results are compatible with expected presence of endogenous photosensitizers (porphyrins) in the bacteria.

Quantum illumination with noisy probes: Conditional advantages of non-Gaussianity

Entangled states, like the two-mode squeezed vacuum state, are known to give quantum advantage in the illumination protocol, a method to detect a weakly reflecting target submerged in a thermal background. We use non-Gaussian photon-added and -subtracted states, affected by local Gaussian noise on top of the omnipresent thermal noise, as probes in the illumination protocol. Based on the difference between the Chernoff bounds obtained with the coherent state and the non-Gaussian state having equal signal strengths, whose positive values denote quantum advantage in illumination, we highlight the hierarchy among non-Gaussian states, which is compatible with correlations per unit signal strength, although the Gaussian states offer the best performance. Interestingly, such hierarchy is different when comparisons are made using the Chernoff bounds. The entire analysis is performed in the presence of different imperfect apparatus like faulty twin-beam generator, imperfect photon addition (subtraction) as well as with noisy non-Gaussian probe states.

Detection scheme using a beam splitter and on-off detectors for non-Gaussian state-based quantum illumination with attenuation

Quantum illumination is an entanglement-based protocol for target detection. The use of a two-mode squeezed vacuum (TMSV) state as a type of Gaussian state has been widely discussed. In our previous study, we found that the quasi-Bell state, which is a non-Gaussian state, outperforms the TMSV state in the quantum illumination protocol with attenuation. In this paper, we construct a detection scheme using practical elements and demonstrate that it can outperform the TMSV state without using the photon-number resolution.

Time alignment quantum illumination based on single real-time coincidence counting

We propose and demonstrate an improved quantum illumination protocol based on the time correlation of twin photons, for the high signal-to-noise ratio (SNR) of target detection and signal reconstruction in the strong noise environment. The Hong-Ou-Mandel (HOM) interferometer is applied after the spontaneous parametric down-conversion (SPDC) process to construct a probing twin-beam in which the photon times are precisely aligned between the beams. At the radar receiver, we put forward a single real-time coincidence counting (SRCC) method on a series of time slices to reconstruct the probe signals of pulse radar and calculate the SNR advantages against the conventional pulse radar, as well as the quantum illumination (QI) protocol. Our main achievements in this research are the realization of real-time detection of quantum information while acquiring a higher SNR than QI and classical illumination (CI) protocols, as well as its demonstration of strong robustness to noise and losses, which also proposes what we believe to be a novel way for quantum target detection.

PSI Photoinhibition and Changing CO2 Levels Initiate Retrograde Signals to Modify Nuclear Gene Expression.

Photosystem I (PSI) is a critical component of the photosynthetic machinery in plants. Under conditions of environmental stress, PSI becomes photoinhibited, leading to a redox imbalance in the chloroplast. PSI photoinhibition is caused by an increase in electron pressure within PSI, which damages the iron-sulfur clusters. In this study, we investigated the susceptibility of PSI to photoinhibition in plants at different concentrations of CO2, followed by global gene expression analyses of the differentially treated plants. PSI photoinhibition was induced using a specific illumination protocol that inhibited PSI with minimal effects on PSII. Unexpectedly, the varying CO2 levels combined with the PSI-PI treatment neither increased nor decreased the likelihood of PSI photodamage. All PSI photoinhibition treatments, independent of CO2 levels, upregulated genes generally involved in plant responses to excess iron and downregulated genes involved in iron deficiency. PSI photoinhibition also induced genes encoding photosynthetic proteins that act as electron acceptors from PSI. We propose that PSI photoinhibition causes a release of iron from damaged iron-sulfur clusters, which initiates a retrograde signal from the chloroplast to the nucleus to modify gene expression. In addition, the deprivation of CO2 from the air initiated a signal that induced flavonoid biosynthesis genes, probably via jasmonate production.

Evaluation on quantum illumination radar with quantum limited amplification.

Based on Quantum illumination (QI) protocol, researcshers have developed prototypes of quantum radar and demonstrated its quantum enhancement. Nevertheless, there are still difficulties in the practical application for QI radar, especially the trade-off between the detection range and quantum enhancement, as well as the construction of the optimized receiver. Some studies have suggested that the potential solutions to these difficulties are to deploy the quantum limited amplifiers in QI radars, and have envisioned different amplification schemes. In this paper, we establish a universal and effective method to evaluate the signal-to-noise ratio of QI radar. It connects QI radar theory with classical radar signal processing theory, providing support for researchers to evaluate the performance of various QI radar schemes from a radar perspective. Based on this method, we prove that any quantum limited phase-insensitive amplification scheme will seriously weaken the quantum enhancement of QI radar. Furthermore, we also demonstrate that the QI radar with phase-sensitive amplified idler has no advantage over the optimal classical illumination. These results can help us avoid some unreasonable QI radar schemes. In addition, we believe that the proposed method can also be applied to explore other potential QI radar schemes and contribute to promoting the application development of QI radar.

Evaluating the detection range of microwave quantum illumination radar

AbstractBased on quantum illumination (QI) protocol, microwave quantum radar has been developed, which has attracted great interest from radar researchers. While, according to the QI protocol, the quantum advantage of QI radar is related to the power of generated signal, which may lead to a trade‐off between its quantum advantage and the maximum detection range. However, the maximum detection range is an important indicator to measure the performance of the radar. Therefore, it is necessary to quantitatively analyse the maximum detection range and quantum advantages of microwave QI radar, which will help us understand its performance from the perspective of radar applications and further evaluate the potential application scenarios and future research directions of it. The microwave quantum radar equation is developed, by the authors, based on the classical radar equation with the consideration of the quantum properties of extremely weak signals. Then, the maximum detection range of QI radar and classical two mode noise radar in different microwave bands is comprehensively compared and analysed. The possible applications of microwave QI radars in different bands are also discussed. In addition, the authors demonstrate that the symmetrical quantum limited amplification actually has no contribution to improving the detection range of a QI radar.

Ketamine affects homeostatic sleep regulation in the absence of the circadian sleep-regulating component in freely moving rats

Pharmacological effects of ketamine may affect homeostatic sleep regulation via slow wave related mechanisms.In the present study effects of ketamine applied at anesthetic dose (80 mg/kg) were tested on neocortical electric activity for 24 h in freely moving rats. Ketamine effects were compared to changes during control (saline) injections and after 6 h gentle handling sleep deprivation (SD). As circadian factors may mask drug effects, an illumination protocol consisting of short light-dark cycles was applied.Ketamine application induced a short hypnotic stage with characteristic slow cortical rhythm followed by a long-lasting hyperactive waking resulting pharmacological SD. Coherence analysis indicated an increased level of local synchronization in broad local field potential frequency ranges during hyperactive waking but not during natural- or SD-evoked waking. Both slow wave sleep and rapid eye movement sleep were replaced after the termination of the ketamine effect.Our results show that both ketamine-induced hypnotic state and hyperactive waking can induce homeostatic sleep pressure with comparable intensity as 6 h SD, but ketamine-induced waking was different compared to the SD-evoked one. Both types of waking stages were different compared to spontaneous waking but all three types of wakefulness can engage the homeostatic sleep regulating machinery to generate sleep pressure dissipated by subsequent sleep. Current-source density analysis of the slow waves showed that cortical transmembrane currents were stronger during ketamine-induced hypnotic stage compared to both sleep replacement after SD and ketamine application, but intracortical activation patterns showed only quantitative differences.These findings may hold some translational value for human medical ketamine applications aiming the treatment of depression-associated sleep problems, which can be alleviated by the homeostatic sleep effect of the drug without the need for an intact circadian regulation.

Feedback-enabled microwave quantum illumination

A simple feedback scheme can be used to operate efficiently a microwave-quantum-illumination device based on electro-optomechanical systems also in regimes in which excess dissipation would, otherwise, prevent to outperform the optimal classical illumination protocol with the same transmitted energy.

Noise rejection through an improved quantum illumination protocol

Quantum illumination protocols can be implemented to improve imaging performance in the low photon flux regime even in the presence of both background light and sensor noise. However, the extent to which this noise can be rejected is limited by the rate of accidental correlations resulting from the detection of photon or noise events that are not quantum-correlated. Here we present an improved protocol that rejects up to gtrsim 99.9% of background light and sensor noise in the low photon flux regime, improving upon our previous results by an order of magnitude. This improvement, which requires no information regarding the scene or noise statistics, will enable extremely low light quantum imaging techniques to be applied in environments previously thought difficult and be an important addition to the development of covert imaging, quantum microscopes, and quantum LIDAR.

Dynamic Target Detection and Tracking Based on Quantum Illumination LIDAR

In the detection process of classic radars such as radar/lidar, the detection performance will be weakened due to the presence of background noise and loss. The quantum illumination protocol can use the spatial correlation between photon pairs to improve image quality and enhance radar detection performance, even in the presence of loss and noise. Based on this quantum illumination LIDAR, a theoretic scheme is developed for the detection and tracking of moving targets, and the trajectory of the object is analyzed. Illuminated by the quantum light source as Spontaneous Parametric Down-Conversion (SPDC), an opaque target can be identified from the background in the presence of strong noise. The static objects obtained by classical and quantum illumination are compared, respectively, and the advantages of quantum illumination are verified. The moving objects are taken at appropriate intervals to obtain the images of the moving objects, then the images are visualized as dynamic images, and the three-frame difference method is used to obtain the target contour. Finally, the image is performed by a series of processing on to obtain the trajectory of the target object. Several different motion situations are analyzed separately, and compared with the set object motion trajectory, which proves the effectiveness of the scheme. This scheme has potential practical application value.

Photoautotrophic hydrogen production by nitrogen-deprived Chlamydomonas reinhardtii cultures

In this study we have demonstrated the possibility of phototrophic hydrogen production in C. reinhardtii under N-deprived conditions. When tested under air + CO2, and Ar + CO2 N-deprived C. reinhardtii demonstrated decrease in PSII activity mainly due to over reduction of PQ, in addition no ascorbate accumulation was observed in cells. Under air + CO2 atmosphere cells accumulated excessive amounts of starch. When incubated under Ar + CO2 atmosphere cells accumulated starch as nitrogen replete cultures and no hydrogen production was observed. Hydrogen production (86 ml H2 per one l of culture) occurred under Ar + CO2 atmosphere when particular two-step illumination protocol was implicated. In oxygen producing and early oxygen consuming stage cells were illuminated under light intensity 169 μE m−2 s−1. When light was switched to 30 μE m−2 s−1, cultures quickly respired all oxygen and transient to anaerobic conditions with subsequent hydrogen production 2 h later. Actual quantum yield of C. reinhardtii cultures was measured in photobioreactor and maximal quantum efficiency of PSII of dark adapted cells together with JIP test were studied.

Progress Toward an All-Microwave Quantum Illumination Radar

Quantum illumination has been suggested as a way to improve the sensitivity of remote target detection by taking advantage of quantum entanglement between a traveling electromagnetic beam of light interrogating the target, and a second beam kept in the lab. In the microwave domain, superconducting quantum circuit utilizing superconducting Josephson tunnel junctions can be used as quantum-limited amplifiers of faint microwave signals, as well as bright sources of quantum entangled signals. These Josephson parametric amplifiers (JPAs) are poised to become fundamental building block in a future quantum illumination radar operating in the microwave domain. Demonstrating a practical advantage of quantum illumination with ambient temperature signals is, however, challenging. Indeed, current quantum protocols are not practical as they require a complex receiver with stringent phase-matching conditions, which cannot be met in the field. Notably, it is also not currently known how one can transfer faint microwave signals out of cryogenic systems without destroying their fragile entanglement. In this article, we discuss the recent progress and challenges regarding the development of an all-microwave quantum illumination radar using the recently developed and implemented quantum-enhanced noise radar protocol. This quantum illumination protocol is simpler to implement and procures a quantum enhancement over its classical analog. Finally, we discuss different strategies that may resolve the issue of transmitting microwave entanglement under ambient conditions. We, thus, conclude that successful transmission of entangled microwaves can be done in the near future, with possible demonstrations of practical quantum advantage over short distances.

Joint measurement of time\u2013frequency entanglement via sum frequency generation

We propose, analyze, and evaluate a technique for the joint measurement of time–frequency entanglement between two photons. In particular, we show that the frequency sum and time difference of two photons could be simultaneously measured through the sum-frequency generation process, without measuring the time or frequency of each individual photon. We demonstrate the usefulness of this technique by using it to design a time–frequency entanglement based continuous variable superdense coding and a quantum illumination protocol. Performance analysis of these two protocols suggests that the joint measurement of strong time–frequency entanglement of non-classical photon pairs can significantly enhance the performance of joint-measurement based quantum communication and metrology protocols.

A prospective, randomized, within-subject study of ALA-PDT for actinic keratoses using different irradiation regimes.

Photodynamic therapy (PDT) can be used to treat large fields of actinic keratoses (AKs) with high clearance rates. A notable downside is the amount of pain that accompany the treatment. This study aimed to optimize the illumination protocol during conventional PDT in order to reduce pain without compromising treatment effectiveness. In this prospective, randomized study with a split-face design, patients with, symmetrically distributed AKs were included. All patients were treated using a ALA 78mg/g gel. One side was illuminated with the Aktilite® CL-128 lamp and the other side with the RhodoLED® lamp in which the light intensity gradually increased to a maximum of 60%. Both sides received a total light dose of 37J/cm2 . Pain during the treatment was measured using a visual analogue scale. The clinical effectiveness of the 2 treated sides was assessed after 12weeks. Twenty-nine patients with 399 AKs were included. Illumination with the gradually increasing light intensity resulted in a decrease in the median visual analogue scale score by 1.1 points. Clearance rates were similar between the 2 lamps. Minimizing the light intensity during the illumination phase of PDT reduces pain, while still preserving a high clearance rate of AKs.

All-Optical Electrophysiology for High-Throughput Functional Characterization of a Human iPSC-Derived Motor Neuron Model of ALS.

SummaryHuman induced pluripotent stem cell (iPSC)-derived neurons are an attractive substrate for modeling disease, yet the heterogeneity of these cultures presents a challenge for functional characterization by manual patch-clamp electrophysiology. Here, we describe an optimized all-optical electrophysiology, “Optopatch,” pipeline for high-throughput functional characterization of human iPSC-derived neuronal cultures. We demonstrate the method in a human iPSC-derived motor neuron (iPSC-MN) model of amyotrophic lateral sclerosis (ALS). In a comparison of iPSC-MNs with an ALS-causing mutation (SOD1 A4V) with their genome-corrected controls, the mutants showed elevated spike rates under weak or no stimulus and greater likelihood of entering depolarization block under strong optogenetic stimulus. We compared these results with numerical simulations of simple conductance-based neuronal models and with literature results in this and other iPSC-based models of ALS. Our data and simulations suggest that deficits in slowly activating potassium channels may underlie the changes in electrophysiology in the SOD1 A4V mutation.

Changes of pigments and lipids composition in Haematococcus pluvialis vegetative cell as affected by monochromatic red light compared with white light

Much attention has been paid on studies of astaxanthin accumulation process in Haematococcus pluvialis industry. However, growth of H. pluvialis in motile vegetative stage is still the most important and problematic part in the whole cultivation process, such as low growth rate and cell yields. Motile vegetative cells are extremely sensitive to various stresses which make it difficult to maintain the cells of this state to grow. Previous reports showed that motile vegetative cells may have higher biomass yields if applied monochromatic red light. However, metabolic responses of these cells are not completely understood, which constraints application of this illumination protocol in industry. The aim of this study was to examine how critical biochemical changes of H. pluvialis motile vegetative cells were affected by red light when compared with white light. Variation of photosynthetic pigments composition and lipids were mainly studied. By comparing growth process of cultures in red light and white light, prominent variation of pigments composition and lipids changes were observed. The results showed that, even though cell proliferation was the same during exponential growth phase, variation of photosynthetic pigment composition and lipids occurred. The final biomass of cell number was higher in red light group than in white light group. The variations were significant different. Increase or decrease of major photosynthetic membrane lipids to some extent did not influence photosynthesis of the vegetative cells during this phase. However, vegetative cells under polychromatic white light other than monochromatic red light need further metabolic process to adjust its pigment composition and lipids, possibly this is energetically and biochemically unfavorable for motile vegetative cells to growth under white light, a light condition normally not considered as a stress.

Quantum illumination reveals phase-shift inducing cloaking

In quantum illumination entangled light is employed to enhance the detection accuracy of an object when compared with the best classical protocol. On the other hand, cloaking is a stealth technology based on covering a target with a material deflecting the light around the object to avoid its detection. Here, we propose a quantum illumination protocol especially adapted to quantum microwave technology. This protocol seizes the phase-shift induced by some cloaking techniques, such as scattering reduction, allowing for a 3 dB improvement in the detection of a cloaked target. The method can also be employed for the detection of a phase-shift in bright environments in different frequency regimes. Finally, we study the minimal efficiency required by the photocounter for which the quantum illumination protocol still shows a gain with respect to the classical protocol.

Quantum Estimation Methods for Quantum Illumination.

Quantum illumination consists in shining quantum light on a target region immersed in a bright thermal bath with the aim of detecting the presence of a possible low-reflective object. If the signal is entangled with the receiver, then a suitable choice of the measurement offers a gain with respect to the optimal classical protocol employing coherent states. Here, we tackle this detection problem by using quantum estimation techniques to measure the reflectivity parameter of the object, showing an enhancement in the signal-to-noise ratio up to 3dB with respect to the classical case when implementing only local measurements. Our approach employs the quantum Fisher information to provide an upper bound for the error probability, supplies the concrete estimator saturating the bound, and extends the quantum illumination protocol to non-Gaussian states. As an example, we show how Schrödinger's cat states may be used for quantum illumination.

Quantifying the source of enhancement in experimental continuous variable quantum illumination

A quantum illumination protocol exploits correlated light beams to enhance the probability of detection of a partially reflecting object lying in a very noisy background. Recently a simple photon-number-detection based implementation of a quantum illumination-like scheme has been provided in [Lopaeva {\it et al,}, Phys. Rev. Lett. {\bf 101}, 153603 (2013)] where the enhancement is preserved despite the loss of non-classicality. In the present paper we investigate the source for quantum advantage in that realization. We introduce an effective two-mode description of the light sources and analyze the mutual information as quantifier of total correlations in the effective two-mode picture. In the relevant regime of a highly thermalized background, we find that the improvement in the signal-to-noise ratio achieved by the entangled sources over the unentangled thermal ones amounts exactly to the ratio of the effective mutual informations of the corresponding sources. More precisely, both quantities tend to a common limit specified by the squared ratio of the respective cross-correlations. A thorough analysis of the experimental data confirms this theoretical result.