This study analyzes multi-depth groundwater level data and integrated observation data to validate the previously proposed mechanisms of hydrological anomalies triggered by the 2016 M6.4 Meinong earthquake in Taiwan. The main influence area was northwest of the epicenter, which may be due to the blind fault rupture, intensity distribution, and hydrogeological properties. The step changes in groundwater level do not fit the concept of epicentral distance, static stress–strain theory, or the focal mechanism. The distribution of step changes in groundwater level have a pattern similar to that of horizontal peak ground velocity. The results imply that these changes may be driven by dynamic stress–strain, instead of static stress–strain. The minimum horizontal peak ground velocity and acceleration of the Meinong earthquake, which induced obvious step changes in groundwater level and soil liquefaction, are provided. The pressure dissipation ability of an aquifer (e.g., transmissivity) may affect the persistence of groundwater responses. Four wells located near the surface rupture area, which has cemented or partial cemented geological material, showed an obvious step decrease in the groundwater level at a deep depth and all wells showed an increase in the groundwater level at a shallow depth. These decreases and increases of the groundwater level at different depths have different mechanisms, which are discussed in this study. The integrated observations made during the Meinong earthquake show that ground motion and the hydraulic properties might be important factors in hydrological anomalies. The results of this study are an important reference for further studies on earthquake hydrology.
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