Sequential controlled H₂S/CO dual delivery via a self-reporting fluorogenic donor synergistically attenuates myocardial ischemia-reperfusion injury.

Novel flavone-based near-infrared fluorescent probe for the detection of H2S in vitro and in vivo.

Cystathionine γ-lyase (CTH) is markedly enriched in glioblastoma (GBM) and is associated with poor patient survival, enhanced temozolomide (TMZ) resistance, and aggressive phenotypes; however, effective CTH inhibitors for GBM therapy are currently lacking. Using click chemistry-based target identification, we identified cystathionine γ-lyase (CTH) as the direct molecular target of a novel 4-trifluoromethylquinoline derivative, TKL002. TKL002 exhibits strong antitumor activity both in vitro and in vivo, inducing late-stage apoptosis and G2/M cell cycle arrest. Mechanistically, TKL002 inhibits CTH activity, reduces hydrogen sulfide (H2S) production, suppresses NF-κB phosphorylation, and downregulates pro-inflammatory cytokine expression. In addition, TKL002 inhibits GBM cell migration and invasion by upregulating E-cadherin and downregulating N-cadherin and vimentin. Collectively, these findings demonstrate that TKL002 exerts potent antiglioblastoma activity via modulation of the CTH/H2S/NF-κB/EMT signaling axis, highlighting its potential as a quinoline-based therapeutic candidate to overcome intrinsic GBM resistance and invasiveness.

This study investigates the production of biogas through the co-anaerobic digestion of cow dung and sawdust, utilizing thermochemical pretreatment to enhance lignin breakdown. A 50:50 and a 75:25 mixture of the substrates (Cowdung:sawdust) were subjected to sodium hydroxide pretreatment and thermal conditioning at 80°C. Lignin content reduced from 31.94% to 22.73%. The results demonstrated approximately a 43% increase in biogas yield for both the 50:50 and 75:25 substrate ratios. A four-day earlier gas production onset was recorded for pretreated substrates compared to untreated samples. The methane content of the biogas reached 56% (50:50 ratio) and 60% (75:25 ratio), with hydrogen sulfide at about 1% in both ratios. Process parameters such as pH, and temperature were measured. This study provides a scalable approach for waste-to-energy applications and demonstrates the role of pretreatment in improving substrate digestibility and biogas yield.

Sulfur metabolism regulates endoplasmic reticulum stress survival through the interaction between cystathionine beta-synthase and Sec14 protein.

Hydrogen production from hydrogen sulfide via a uniquely designed electrolysis process: experimental investigation

Gas-based therapeutics and delivery platforms in cancer immunotherapy.

Exogenous hydrogen sulfide inhibits the progression of vascular dysfunction and hypertension mediated by the renin-angiotensin system in chronic kidney disease.

Triple-Channel fluorescent probe for simultaneous detection of Cys, SO₂, and H₂S to discriminate Cancer cells via Transsulfuration pathway metabolic profiling.

Multifunctional self-cascade nanoplatform for efficient immunotherapy through synergizing pyroptosis and self-augmented activation of cGAS-STING pathway.

A novel fluorescent probe for in vivo H₂S monitoring in inflammatory arthritis.

Hydrogen sulfide reduces omeprazole and is oxidized to polysulfides and elemental sulfur.

Hydrogen sulfide (H2S), classified as a Class III gaseous signaling molecule, plays a critical role in regulating a range of physiological and pathological processes, including vasodilation, oxidative stress balance, synaptic plasticity, and tumor metastasis. In recent years, the development of high-efficiency, high-sensitivity fluorescent probes has garnered significant attention due to their advantages in spatial and temporal resolution, minimal background interference, and capacity for deep tissue imaging. These characteristics have positioned fluorescent probes as essential tools for elucidating the dynamic distribution and underlying mechanisms of H2S activity. This review systematically examines recent progress in near-infrared fluorescent probes, particularly those based on nucleophilic substitution, reduction reactions, and copper sulfide precipitation. The discussion emphasizes innovations in detection mechanisms, performance enhancements, and applications in biological systems. These advancements provide a theoretical foundation for a deeper understanding of H2S biology and support the development of integrated diagnosis and treatment tools, thereby advancing the application of H2S probes in life sciences, environmental monitoring, and clinical translation.

A hemicyanine dye fluorescent probe for the detection of H2S in food spoilage and living cells.

Hydrogen sulfide revisited: a stealthy ally in the unseen war against cardiovascular disease.

A thiol-activated near-infrared fluorescent H2S donor for visualizing H2S release and its applications.

Hydrogen sulphide as a potential contributer to cardiovascular protection with sodium-glucose cotransporter 2 inhibition.

Unraveling the role of exogenous microbial inoculant in shifting microbial networks and facilitating sulfur transformation pathways to hydrogen sulfide during food waste biogas residue composting

Potential for microbial methanethiol-dependent dimethylsulfide production in different marine sediments.

Enhancement of postharvest quality and antioxidative ability in broccoli sprouts by preharvest application of hydrogen sulfide