Hunan Research Articles

Mediating role of inner strength in the relationship between medication literacy and medication adherence among kidney transplant patients.

Compared with long-term renal replacement therapy, kidney transplantation is the ideal treatment for end-stage renal disease (ESRD), significantly extending patient life and improving quality of life. Kidney transplant patients need to adhere to lifelong immunosuppressive medication regimens, but their medication adherence is generally poor compared with other organ transplant recipients. Medication adherence is closely related to medication literacy and psychological status, yet related studies are limited. This study aims to investigate the current status of medication adherence, inner strength, and medication literacy in kidney transplant patients, analyze the relationships among these 3 factors, and explore the mediating role of inner strength in the relationship between medication literacy and medication adherence. A cross-sectional survey was conducted from March to October 2023 involving 421 patients aged≥18 years who visited kidney transplantation outpatient clinics at 4 tertiary hospitals in Hunan Province. The inner strength, medication literacy, and medication adherence of kidney transplant patients were investigated using the Inner Strength Scale (ISS), the Chinese version of the Medication Literacy Assessment in Spanish and English (MedLitRxSE), and the Chinese version of the Morisky Medication Adherence Scale-8 (C-MMAS-8), respectively. Univariate analysis was performed to examine the effects of demographic and clinical data on medication adherence. Correlation analysis was conducted to explore the relationships among medication literacy, medication adherence, and inner strength. Significant variables from univariate and correlation analyses were further analyzed using multiple linear regression, and the mediating effect of inner strength was explored. Among the 421 questionnaires collected, 408 were valid, with an effective rate of 96.91%. The scores of C-MMAS-8, MedLitRxSE, and ISS were 6.64±1.16, 100.63±14.67, and 8.47±4.03, respectively. Among the 408 patients, only 86 (21.08%) patients had a high level of medication adherence, whereas 230 (56.37%) patients had a medium level of medication adherence, and 92 (22.55%) patients had poor medication adherence. Univariate analysis indicated that the kidney transplant patients' age, marital status, education levels, years since their kidney transplant operation, number of hospitalizations after the kidney transplant, and adverse drug reactions showed significant differences in medication adherence (all P<0.05). Correlation analysis showed that inner strength positively correlated with both medication literacy (r=0.183, P<0.001) and medication adherence (r=0.201, P<0.001). Additionally, there was a positive correlation between medication adherence and medication literacy (r=0.236, P<0.001). Inner strength accounted for 13.22% of the total effect in the mediating role between medication literacy and medication adherence. The level of medication adherence among kidney transplant patients needs improvement, and targeted intervention measures are essential. Inner strength mediates the relationship between medication literacy and medication adherence in these patients. Healthcare professionals should focus on enhancing medication literacy and supporting patients' inner strength to improve medication adherence.

Influence of soil properties on selenium concentration in paddy soil and rice grains in the hilly regions of southern China

BackgroundSelenium (Se) is essential for human health and is predominantly obtained from dietary sources, particularly rice in Hunan Province, a significant rice-producing region in southern China. Investigating the relationship between Se levels in paddy soil and rice grains, along with the associated influencing factors, is critical for enhancing Se-enriched food security.ResultsAnalysis of 128,992 samples collected between 2019 and 2022 revealed that the soil Se concentration in Hunan exceeded the global average, with rice grains showing promising potential for Se enrichment. Various analytical methods, including statistical analyses, co-occurrence networks, and correlation heatmaps, were utilized to scrutinize the extensive dataset. Additionally, partial least squares path analysis elucidated the interactive effects of influencing factors on soil Se concentration, rice grain Se concentration, and Se bioconcentration factor (BCF). Soil parent materials significantly affected soil Se concentration, rice grain Se concentration, and Se BCF (p < 0.01). Factors such as soil cation exchange capacity, soil organic matter, slope, and soil concentrations of Cu, Mn, and Zn demonstrated positive correlations with soil Se concentration. Similarly, these factors exhibited positive associations with rice grain Se concentration. Conversely, negative correlations were observed between certain factors and Se BCF. As a result, predictive models were developed for soil Se, rice grain Se concentration, and Se BCF.ConclusionsThis study contributes valuable insights to inform policy-making for Se-enriched food production and to ensure regional nutritional equilibrium. Caution is recommended in areas with excessive Se levels to prevent potential poisoning risks.

Knowledge mapping of job burnout and satisfaction of medical staff and a cross-sectional investigation of county-level hospitals in Southern China

BackgroundJob burnout is a worldwide public health problem that has rarely been addressed among rural medical staff, particularly in county-level hospitals. Hence, we conducted a bibliometric study to gain global insights and research trends and a cross-sectional study to assess the current situation among medical staff of county-level hospitals in Southern China. By conducting these studies, we aim to identify factors associated with burnout among medical staff of county-level hospitals in China and provide recommendations for improvement. MethodsRelevant literature on job burnout among medical personnel was searched using the Web of Science Core Collection (WoSCC). CiteSpace was employed for an in-depth cluster analysis to determine research trends and identify the study population. Subsequently, a cross-sectional survey was randomly conducted in three county-level hospitals in Hunan Province of Southern China. Job burnout and satisfaction were assessed using the Maslach Burnout Inventory MBI-Human Services Survey (MBI-HSS) and Job Satisfaction Questionnaire in a total of 362 valid questionnaires collected. The influence factors of the prevalence of job burnout were investigated using logistic regression. ResultsIn this bibliometric study, 1626 articles were retrieved from 1999 to 2022. China lags behind the United States (US) in both the number and quality of publications in the field of medical staff burnout compared with the US. However, there is a lack of comparative research on job burnout across different job types. County-level medical staff articles are more in line with research hotspots in the field. In total, 362 valid questionnaires were obtained. The total incidence of job burnout among rural medical staff was 27.3 %. Nurses (p < 0.01, OR = 5.95), doctors (p < 0.01, OR = 6.43), and those with administrative jobs (p < 0.01, OR = 7.79) were more likely to experience burnout than those with technical jobs. Medical staff aged 40–49 years (p < 0.01, OR = 0.22) and 50–59 years (p < 0.05, OR = 0.14) were less likely to experience burnout than those aged 20–29 years. Job rewards satisfaction showed a positive correlation with job burnout (p < 0.01, OR = 1.32), but negative correlations with personal development satisfaction (p < 0.05, OR = 0.81) and work internal environment satisfaction (p < 0.05, OR = 0.81). ConclusionBetter working environments, more accessible resources, and higher job rewards contribute to job satisfaction and reduce job burnout among the medical staff of county-level hospitals in China.

Ecological Adaptation and Sustainable Cultivation of Citrus reticulata by Applying Mixed Design Principles under Changing Climate in China

Climate change is heavily altering plant distributions, posing significant challenges to conventional agricultural practices and ecological balance. Meanwhile, mixed species planting emerges as a potent strategy to enhance agricultural resilience, counteract climate change, preserve ecological balance, and provide a solution to economic instability. The MaxEnt model was used to predict the suitable area of Citrus reticulata under five climate scenarios and to explore affecting environmental factors. Litchi chinensis, Punica granatum, and Lycium chinense were selected as mixed species to analyze the spatial distribution and centroid migration trend of potentially suitable areas. The research results show the following: (1) The primary environmental factors impacting C. reticulata distribution are annual precipitation (1000–4000 mm), precipitation of driest quarter over 100 mm, and mean temperature of coldest quarter (12–28 °C). Crucially, the mixed species exhibited similar environmental sensitivities, indicating mutual mixing suitability. (2) Currently, the C. reticulata suitable area is of 240.21 × 104 km2, primarily in South, East, Central, and Southwest China, with potential for expansion to 265.41 × 104 km2 under the 2090s SSP1-2.6 scenario. (3) The geometric center of the moderately-to-highly suitable areas for C. reticulata is located in Hunan Province. Future scenarios show the C. reticulata’s centroid migrating northwest, with distances of less than 110 km. Mixed planting trends toward higher latitudes, fluctuating from 6 km to 210 km. (4) Mixed planting area planning: C. reticulata and L. chinensis are suitable for mixed planting in South China. C. reticulata and P. granatum, C. reticulata and L. chinense are suitable for mixed planting in most areas of Central, East, Southwest, and South China. This research presents a new perspective on using mixed design principles for ecological adaptation and the sustainable mixed planting of C. reticulata, in response to China’s changing climate. This approach is expected to help the economic fruit tree industry enhance ecological resilience and economic stability in the face of future climate change challenges.

Description of a new species of Andricus Hartig, 1840 (Hymenoptera: Cynipidae: Cynipini) from China

A new species of gall wasp, Andricus wugangensis Zeng, Liu, &amp; Zhu sp. nov. is described and illustrated herein from Hunan Province, China. The new species is most similar to A. wuhanensis Ide, Abe, Su &amp; Zhu and A. xishuangbanaensis Melika &amp; Tang in morphology but can be easily distinguished by having 1) a large V-shaped carina on the lower face and 2) a broad transverse depression spanning the region between the inner margin of the eyes on the upper face. The results of a phylogenetic analysis and pairwise genetic distance comparison, based on COI sequences, were consistent with the conclusion of the comparative morphological assessment of the similar species: A. wuganensis, A. wuhanensis and A. xishuangbanaensis, although the morphological differences are more obvious than the small genetic distance of the COI sequences, which is 4.3% and 3% between the new species and A. wuhanensis and A. xishuangbanaensis, respectively. Additionally, a taxonomic key to the known species of Andricus from China is provided.

Prevalence and death rate of birth defects from population-based surveillance in Hunan Province, China, 2010–2020

To describe the prevalence and death rate of birth defects from population-based surveillance in Hunan Province, China. Data were obtained from the population-based Birth Defects Surveillance System in Hunan Province, China (2010–2020). The surveillance population included all live births, stillbirths, infant deaths, and legal terminations of pregnancy from 28 weeks of gestation to 42 days after birth between 2010 and 2020 when the mother resided in the surveillance area (Liuyang County and Shifeng District, Hunan Province). The prevalence of birth defects is the number of birth defects per 1000 infants (‰). The death rate of birth defects is the number of deaths attributable to birth defects per 100 birth defects (%). The prevalence and death rate with 95% confidence intervals (CI) were calculated by the log-binomial method. Crude odds ratios (ORs) were calculated to examine the association of each demographic characteristic with birth defects. Our study included 228,444 infants, and 4453 birth defects were identified, with a prevalence of 19.49‰ (95%CI 18.92–20.07). Congenital heart defects were the most common specific defects (5.29‰), followed by limb defects (4.01‰). Birth defects were more common in males than females (22.34‰ vs. 16.26‰, OR = 1.38, 95%CI 1.30–1.47), in premature birth than not (91.82‰ vs. 16.14‰, OR = 6.16, 95%CI 5.72–6.65), in birth weight < 2500 g (98.26‰ vs. 16.22‰, OR = 6.61, 95%CI 6.11–7.15) or > 4000 g (19.48‰ vs. 16.22‰, OR = 1.21, 95%CI 1.03–1.42) than birth weight 2500–4000 g, in hospitalized deliveries than other institutions (22.16‰ vs. 11.74‰, OR = 1.91, 95%CI 1.76–2.07), in multiple births than singletons (28.50‰ vs. 19.28‰, OR = 1.49, 95%CI 1.27–1.76), in maternal age < 20 years (26.33‰ vs. 18.69‰, OR = 1.42, 95%CI 1.15–1.76) or > = 35 years (24.31‰ vs. 18.69‰, OR = 1.31, 95%CI 1.18–1.45) than maternal age 25–29 years, and in number of pregnancies > = 4 (22.91‰ vs. 18.92‰, OR = 1.22, 95%CI 1.10–1.35) than the first pregnancy. A total of 747 deaths attributable to birth defects were identified, including 603 (80.72%) stillbirths, 75 (10.04%) deaths within 7 days after birth, 46 (6.16%) deaths in 7–27 days after birth, 23 (3.08%) deaths in 28–42 days after birth. The death rate of birth defects was 16.78% (95%CI 15.57–17.98). Deaths attributable to birth defects accounted for 51.09% (747/1462) of all deaths. Central nervous system defects had the highest death rate (90.27%), and neonatal genetic metabolic defects had the lowest death rate (0.39%). In summary, we have described the prevalence and epidemiology of birth defects from population-based surveillance in Hunan Province, China, 2010–2020. There were differences in the prevalence and death rate of birth defects between population-based surveillance and hospital-based surveillance.

Enhanced immobilization of cadmium and lead in contaminated soil using calcium alginate-modified HAP biochar: Improvements in soil health and microbial diversity

A novel adsorbent, calcium alginate-modified HAP (Hydroxyapatite)-wood ear mushroom sticks biochar (CA-HAPMB), was synthesized to enhance the immobilization of Cd and Pb in soil. Over 150 days, applying CA-HAPMB at concentrations of 0%–3% in contaminated soils from Chenzhou City in Hunan Province (CZ) and Shenyang City in Liaoning Province (SY) resulted in decreased effective concentrations of Cd and Pb. Specifically, in CZ soil, Cd and Pb decreased by 30.9%–69.3% and 31.9%–78.6%, respectively, while in SY soil, they decreased by 27.5%–53.7% and 26.4%–62.3%, respectively. Characterization results, obtained after separating CA-HAPMB from the soil, indicate that complexation, co-precipitation, and ion exchange play crucial roles in the efficient immobilization of Cd and Pb by CA-HAPMB. Additionally, adjusting the amount of CA-HAPMB added allows modulation of soil pH, leading to increased soil organic matter and nutrient content. Following treatment with CA-HAPMB for immobilizing Cd and Pb, soil bacteria abundance and diversity increased, further promoting heavy-metal immobilization.

Regional Landslide Susceptibility Assessment and Model Adaptability Research

Landslide susceptibility denotes the likelihood of a disaster event under specific conditions. The assessment of landslide susceptibility has transitioned from qualitative to quantitative methods. With the integration of information technology in geological hazard analysis, a range of quantitative models for assessing landslide susceptibility has emerged and is now widely used. To compare and evaluate the accuracy of these models, this study focuses on Xupu County in Hunan Province, applying several models, including the CF model, FR model, CF-LR coupled model, FR-LR coupled model, SVM model, and RF model, to assess regional landslide susceptibility. ROC curves are used to evaluate the reliability of the model’s predictions. The evaluation results reveal that the CF model (AUC = 0.756), FR model (AUC = 0.764), CF-LR model (AUC = 0.776), FR-LR model (AUC = 0.781), SVM model (AUC = 0.814), and RF model (AUC = 0.912) all have AUC values within the range of 0.7–0.9, indicating that the overall accuracy of the models is good and can provide a reference for landslide susceptibility zoning in the study area. Among these, the Random Forest model demonstrates the best accuracy for landslide susceptibility zoning in the study area. By extracting the extremely high susceptibility zones from the landslide susceptibility zonings obtained by six models, a comparative analysis of model adaptability was conducted. The results indicate that the Random Forest model has the best adaptability under specific conditions in Xupu County.

The Influence of Climate Change on the Distribution of Hibiscus mutabilis in China: MaxEnt Model-Based Prediction.

Our study utilized 374 geographical distribution records of H. mutabilis and 19 bioclimatic factors, employing the MaxEnt model and the Geographic Information System (ArcGIS). The key environmental variables influencing the suitable distribution areas of H. mutabilis were analyzed through the comprehensive contribution rate, permutation importance, and Pearson correlation coefficient. Based on this analysis, the contemporary and future suitable distribution areas and their extents were predicted. The results indicate that the key limiting factor affecting the suitable distribution areas of H. mutabilis is the precipitation of the driest month (bio14), with secondary factors being annual precipitation (bio12), annual mean temperature (bio1), and annual temperature range (bio7). Under contemporary climate conditions, the total suitable area for H. mutabilis is approximately 2,076,600 km2, primarily concentrated in the tropical and subtropical regions of southeastern China. Under low-to-medium-emission scenarios (SSP1-2.6, SSP2-4.5), the total suitable area of H. mutabilis shows a trend of first decreasing and then increasing compared to the current scenario. In contrast, under high-emission scenarios (SSP5-8.5), it exhibits a trend of first increasing and then decreasing. The spatial pattern changes indicate that the retention rate of suitable areas for H. mutabilis ranges from 95.28% to 99.28%, with the distribution centers primarily located in Hunan and Guizhou provinces, showing an overall migration trend towards the west and north. These findings suggest that H. mutabilis possesses a certain level of adaptability to climate change. However, it is crucial to consider regional drought and sudden drought events in practical cultivation and introduction processes. The results of our study provide a scientific basis for the rational cultivation management, conservation, and utilization of germplasm resources of H. mutabilis.

The mediating effect of job embeddedness on the nursing work environment and transition shock among new nurses: A cross-sectional study

AimsThis study aims to investigate the status of transition shock and test the mediating effect of job embeddedness on the nursing work environment and transition shock among new nurses. BackgroundTransition shock, primarily experienced during the first two years of employment, manifests itself as an unsettling or discordant experience among new nurses, which is caused by the gap between theoretical learning and clinical practice. DesignThis is a cross-sectional survey study, following the STROBE guidelines. MethodsA cross-sectional study was conducted among 435 new nurses from November to December 2022 in Hunan Province, China. The participants were recruited to complete a sociodemographic information questionnaire and rank 3 standardized scales of transition shock (Cronbach's alpha = 0.918), job embeddedness (Cronbach's alpha = 0.890) and nursing work environment (Cronbach's alpha = 0.946). Then, the data were analysed by SPSS 26.0 and mediation testing was performed using Hayes’ PROCESS macro (Model 4). A p value of < 0.05 was deemed statistically significant. ResultsThe mean score with standard deviation of the transition shock scale in new nurses was 88.86 (22.50). The place of origin, working months and weekly working hours were the significant sociodemographic factors that affected the transition shock score in new nurses. Transition shock was found to be negatively related to both job embeddedness (r = −0.468, p < 0.001) and nursing work environment (r = −0.416, p < 0.001), while job embeddedness mediated the relationship between transition shock and nursing work environment (R2 = 0.262, F = 30.455, p < 0.001). ConclusionsNew nurses from rural areas and with longer weekly working hours are more prone to transition shock. Those with 6–12 months of employment experience more pronounced transition shock than those with less than 6 months. In addition, the nursing work environment can not only directly influence transition shock for new nurses but also indirectly influence it through job embeddedness. These findings provide a basis for hospitals to develop strategies to effectively address transition shock in new nurses and ultimately solve the nursing shortage issue.

Multi-objective optimization method for medium and long-term power supply and demand balance considering the spatiotemporal correlation of source and load

The medium and long-term supply-demand imbalance of the power system in the context of the new power system is becoming more and more prominent due to the fluctuation and intermittency brought about by the high proportion of new energy sources connected to the grid. In this regard, a multi-objective power supply-demand balance optimization method considering the spatiotemporal correlation of source and load is proposed in this work. First, the autocorrelation and inter-correlation characteristics of source and load are analyzed. On this basis, a multi-dimensional scenario set construction method considering the spatiotemporal correlation of source and load is proposed. Then, the planning capacity of each regional power source and the system operation under each scenario are taken as the optimization variables. Renewable energy electricity curtailment, equivalent annual total cost, and inter-region transmission electricity are taken as the optimization objectives. Various constraints such as power source planning and operation, power balance, inter-region power transmission, and renewable energy power curtailment rate are considered comprehensively. The optimization method for the medium and long-term power supply and demand balance is proposed. Finally, the method is applied to Hunan Province, China to guide power planning. The results show that compared with traditional multi-dimensional correlation scene construction methods, the average probability density functions error of wind turbine output, photovoltaic output, and load constructed in this work decrease by 44.08 %, 73.64 %, and 57.54 %, respectively. It takes into account the regional, temporal, temporal autocorrelation, and inter-correlation of the source and load, and has similar characteristics to historical data. Compared with traditional planning that only considers economy, the optimization plan for power supply and demand balance in this work reduces electricity curtailment and inter-region transmission by 97.04 % and 72.71 %, respectively, balancing renewable energy consumption, economy, and regional independent balancing indicators.

Increasing Hybrid Rice Yield, Water Productivity, and Nitrogen Use Efficiency: Optimization Strategies for Irrigation and Fertilizer Management.

Water and fertilizer are crucial in rice growth, with irrigation and fertilizer management exhibiting synergies. In a two-year field study conducted in Yiyang City, Hunan Province, we examined the impact of three irrigation strategies-wet-shallow irrigation (W1), flooding irrigation (W2), and the "thin, shallow, wet, dry irrigation" method (W3)-in combination with distinct fertilizer treatments (labeled F1, F2, F3, and F4, with nitrogen application rates of 0, 180, 225, and 270 kg ha-1, respectively) on rice yield generation and water-fertilizer utilization patterns. The study employed Hybrid Rice Xin Xiang Liang you 1751 (XXLY1751) and Yue Liang you Mei Xiang Xin Zhan (YLYMXXZ) as representative rice cultivars. Key findings from the research include water, fertilizer, variety, and year treatments, which all significantly influenced the yield components of rice. Compared to W2, W1 in 2022 reduced the amount of irrigation water by 35.2%, resulting in a 42.0~42.8% increase in irrigation water productivity and a 25.7~25.9% increase in total water productivity. In 2023, similar improvements were seen. Specifically, compared with other treatments, the W1F3 treatment increased nitrogen uptake and harvest index by 1.4-7.7% and 5.9-7.7%, respectively. Phosphorus and potassium uptake also improved. The W1 treatment enhanced the uptake, accumulation, and translocation of nitrogen, phosphorus, and potassium nutrients throughout the rice growth cycle, increasing nutrient levels in the grains. When paired with the F3 fertilization approach, W1 treatment boosted yields and improved nutrient use efficiency. Consequently, combining W1 and F3 treatment emerged as this study's optimal water-fertilizer management approach. By harnessing the combined effects of water and fertilizer management, we can ensure efficient resource utilization and maximize the productive potential of rice.

The Impact of High-Speed Rail Opening on Urban Economy Empirical Evidence from China

The business scale of China's high-speed railroad is increasing, which has brought important impact on the economy of the cities passing along the high-speed railroad. High-speed railroad has faster speed, safer, and stronger ability to transport goods compared with train, which provides a better choice for people's transportation, tourism, etc., and also has a great impact on the economic development of the cities along the route. This paper is based on the Wuhan-Guangzhou high-speed railway along Hunan Province.To ensure accurate empirical results, we specifically choose Hunan Province as the basis for data analysis, as it is the only province in the area and eliminates potential interference from other municipalities. We collect data from 2005 to 2019, specifically from prefectural-level cities and above. The empirical analysis is conducted using the double difference model (DID). An analysis is conducted on the effects of high-speed railroads on the growth rate of GDP, per capita GDP, the total employment at the end of the year, and the urban development along the lines. The findings indicate that high-speed rail has a discernible bias in its economic impact on cities along its route. Specifically, it has a substantial influence on the employment and urbanisation development of these cities. However, it does not have a significant effect on the short-term per capita GDP and its growth rate.

Nitrogen scavenging by winter fallow weeds and its availability to rice crops

Weedy fallow presents a promising strategy for nitrogen (N) scavenging and reducing N losses from croplands. The study aimed to (1) reassess the N scavenging potential of winter fallow weeds, specifically foxtail grasses, within rice cropping systems and (2) determine the availability of N scavenged by winter fallow weeds in rice paddies to rice crops. A two-year on-farm investigation (2022 and 2023), a one-year 15N-tracing microplot experiment (2021), and a two-year field experiment (2022 and 2023) were conducted in Hunan Province, China. The on-farm investigation revealed that winter fallow weeds scavenged on average 10.6 g N m–2 in 2022 and 10.9 g N m–2 in 2023. In the 15N-tracing microplot experiment, a 24 % average recovery efficiency of N from winter fallow weeds, applied in conjunction with two rice varieties and two urea-N application rates, was observed. The field experiment showed that total N uptake by rice plants and grain yield of rice crops were linearly and quadratically related to the total N application rate (the sum of N in applied winter fallow weeds and urea), respectively, in both 2022 and 2023. These findings highlight the potential of winter fallow weeds in rice paddies to scavenge N effectively and reveal this scavenged N to be highly available to rice crops. This study offers insights into the dual advantages of winter fallow weeds within rice cropping systems, shedding light on their environmental benefits as well as their positive agronomic outcomes.

First report of Fusarium commune Causing Bulb Rot on Lily (Lilium brownii var. Viridulum Baker) in Hunan Province of China.

Lily (Lilium brownii var. Viridulum Baker) is a well-known edible plant with large, white and sweet bulb scales that has important medicinal value (Zhou et al. 2021) and is grown mainly in the Hebei, Shanxi and Henan provinces of China. In May 2021, a case of bulb rot was discovered in a 3.33 hm2 plantation in Huaihua, Hunan Province, affecting 20% of the area (27°59'30″N, 110°32'20″E). The disease is most severe during the rainy season in May and June. In the early stage, irregular brown spots appeared on the lily scales, the necrosis was depressed and gradually enlarges, and in the later stage, the scales were scattered from the base of the disc and slough off. Ten samples were taken randomly from different plants in the plantation area to isolate the pathogens. After washing with sterile water, they were cut into small pieces and sterilised with 3% hydrogen peroxide for 30 s, 75% ethanol for 90 s, rinsed three times with sterile water and dried on sterile filter paper, then placed on a water agar plate and incubated in the dark in a constant temperature incubator at 28℃ for 3 to 5 days. After 2 days, the mycelium at the edge of the colony was transferred to a PDA plate and incubated for 3-5 days at 28°C in the dark to obtain pure fungal isolates. Eighteen purified fungal isolates were obtained, of which sixteen looked like Fusarium (88.9% isolation rate) and three representative isolates (BHBR2, BHBR3 and BHBR5) were selected for further study. The surface of this fungus was white with dense aerial mycelium. Some had an orange centre in the medium. Microconidia were oval in shape and appeared either straight or slightly curved. These microconidia were colourless, had 0-1 septa and measured 3.334 to 14.724 × 2.216 to 5.385 μm (n=100). Macroconidia were predominantly three-septate, crescent-shaped structures that were thin-walled and slightly curved. Cells at the apex and base were similarly curved. Macroconidia measured 17.956 to 32.150 × 2.788 to 4.492 μm (n=100). The mitochondrial small subunit (mtSSU) and translation elongation factor 1-α (TEF1) genes were amplified and sequenced using the NMS1/NMS2 and TEF-R/TEF-F primers to verify the identity of the pathogens (Stewart et al. 2006). The sequences were submitted to GenBank (BHBR2: mtSSU, PP273435; TEF, OR900976; BHBR3: mtSSU, PP277729; TEF, OR900977; BHBR5: mtSSU, PP277728; TEF, OR900978). A concatenated phylogenetic tree of the two genes was constructed and analysis showed that BHBR2, BHBR3 and BHBR5 were significantly clustered with Fusarium commune. Based on the results of morphological identification and phylogenetic tree analysis, the three isolates were identified as Fusarium commune. We carried out pathogenicity tests using two methods, one in which 6 × 6 mm fungal blocks were inoculated on lily (L. brownie var. viridulum Baker) scales and controls inoculated with sterile blocks, and the other in which strain BHBR2 was selected to carry out pathogenicity tests on bulbs of live plants soaked with 50 ml of a 1 × 106 conidial suspension and bulbs of control plants soaked with sterile water, all in three replicates. They were placed in a growth chamber at 28°C and 80% relative humidity, and the scales were moistened with moistened sterile filter paper. After 3 days of rearing treated scales, lesions appeared on lily scales inoculated with mycelial blocks and expanded with time, whereas no lesions appeared on lily scales inoculated with sterile blocks. One month later, whole plants soaked in the spore suspension wilted, while the control plants grew well. The pathogens re-isolated from the diseased tissues had the same morphological characteristics as representative isolates. This confirms Koch's hypothesis. Fusarium commune has been shown to be the most important pathogenic fungus causing root rot in Alfalfa (Medicago sativa) (Yang et al. 2022) and blueberry (Vaccinium uliginosum L.) (Li et al. 2023) in China. To our knowledge, this is the first report of Fusarium commune causing lily bulb rot in the world, which will lay the foundation for future control of lily bulb rot.

Effective intervention of brucellosis prevention in developing countries: A dynamic modelling study

ObjectiveBrucellosis has a considerable impact on human health and the economy in developing countries. In China, the biggest developing country, brucellosis shifted spread of the epidemic from northern to southern regions. Understanding the transmission characteristic of brucellosis on Hunan province, located in central China, is of great significance for successful control. MethodsWe developed a multi-population and multi-route dynamic model (MPMRDM), which is an animal-human-environment coupled model. The model is an extension of the SEIR model, taking into account direct transmission and indirect transmission. We used the model to explore the spread of brucellosis and evaluate the effectiveness of various intervention strategies. ResultsThe animal-to-animal transmission rate was the highest at 5.14 × 10−8, while the environment-to-person transmission rate was the lowest at 9.49 × 10−12. The mean R0 was 1.51. The most effective intervention was taking personal protection, followed by shortening the infection period. Shortening the infection period combined with personal protection is the most effective two-combined intervention strategy. After any comprehensive intervention strategy was implemented, TAR dropped by 90% or more. ConclusionThe results demonstrate that animal transmission route is essential for controlling human brucellosis. Strengthening personal protection, early detection, and early treatment can effectively control the trend of brucellosis. These results can provide an important reference for optimizing brucellosis intervention plans.

Scientific analysis of iron objects excavated from the Guantian Iron Production Site at Sangzhi County, Hunan Province, China

The Guantian site located in Sangzhi County in northwest Hunan Province, China. It is a low-level settlement and possibly an iron casting and processing site that existed during the Han and Jin periods (202 BCE–AD 420) in the Wuling Mountains region. Analysis of the excavated iron objects from the site reveals that they were mainly made from pig iron, malleable cast iron, and low-carbon steel. The metallurgical ceramic fragments may reflect the existence of melting process. Both casting and forging, with evidence of quenching technique were used in the producing process. Decarburization of cast iron in solid state was the main method of obtaining steel. Since during the Eastern Han dynasty (AD 25–220), the aboriginal in the Wuling Mountains region gradually became stronger, the use of iron-casting and processing technology played an important role in promoting regional social development of the Wuling Man (minorities in the Wuling Mountains region).

2-week prevalence and associated factors of fever, diarrhea, and coexisting fever and diarrhea among children aged 6–23 months in rural Hunan Province

Fever and diarrhea are key causes of malnutrition, growth and development disorders, and death among children. At present, most studies on the associated factors of fever and diarrhea in children are concentrated in African and South Asian countries, but relevant research in China is very limited. This study was aimed to analyze the two-week prevalence of fever, diarrhea, and coexisting fever and diarrhea among children aged 6–23 months in rural areas of Hunan Province and to explore the associated factors. The survey data of the Nutrition Improvement Program for Children in Poor Areas (NIPCPA) from 2016 to 2023 was used here. NIPCPA is a cross-sectional survey completed annually in Hunan to collect children’s nutrition and health indicators. The two-week prevalence rates of fever, diarrhea, and coexisting fever and diarrhea among children aged 6–23 months were 12.2% (2066/16,985), 9.6% (1634/16,985), and 3.2% (542/16,985), respectively. Multivariate logistic regression analysis showed the risks of fever, diarrhea, and coexisting fever and diarrhea were higher among younger children. The high educational level of caregivers, effective consumption of Yingyangbao (a complementary food supplement containing iron, zinc, calcium, vitamins A, D, B1, B2, B12, folic acid, and other micronutrients), and complementary feeding meeting minimum dietary diversity and meeting minimum acceptable diet were protective factors against fever in children, with adjusted odds ratios (aORs) of 0.87 (95%CI: 0.78–0.98), 0.78 (0.69–0.87), 0.73 (0.65–0.82), and 0.74 (0.66–0.84), respectively. Effective consumption of Yingyangbao, and complementary feeding meeting the minimum dietary diversity and meeting minimum acceptable diet were protective factors against diarrhea in children, with aORs of 0.72 (95%CI: 0.63–0.83), 0.79 (0.70–0.91), and 0.80 (0.70–0.92), respectively. Effective consumption of Yingyangbao, and complementary feeding meeting the minimum dietary diversity and meeting minimum acceptable diet were protective factors against coexisting fever and diarrhea among children, with aORs of 0.53 (95%CI: 0.43–0.66), 0.71 (0.58–0.89), and 0.70 (0.56–0.88), respectively. Fever, diarrhea, and the coexisting fever and diarrhea affect one in eight, one in ten, and one in thirty children respectively in rural areas of Hunan. Effective interventions should be actively taken, such as improving the education level of caregivers, enhancing their scientific feeding skills for children, and promoting children’s compliance with Yingyangbao consumption, to further reduce the prevalence of fever and diarrhea in children.

Spatial distribution patterns of human resources allocation in maternal and child healthcare institutions in China from 2016 to 2021

BackgroundIn China, economic, urbanization, and policy differences between the eastern and western regions lead to uneven healthcare resources. This disparity is more pronounced in the west due to fewer healthcare personnel per thousand individuals and imbalanced doctor-to-nurse ratios, which exacerbates healthcare challenges. This study examines the spatial distribution of human resources in maternal and child healthcare from 2016 to 2021, highlighting regional disparities and offering insights for future policy development.MethodsThe data were sourced from the “China Health and Family Planning Statistical Yearbook” (2017) and the “China Health and Health Statistics Yearbook” (2018–2022). This study utilized GeoDa 1.8.6 software to conduct both global and local spatial autocorrelation analyses, using China’s administrative map as the base dataset.ResultsFrom 2016 to 2021, there was an upward trend in the number of health personnel and various types of health technical personnel in Chinese maternal and child healthcare institutions. The spatial distribution of these personnel from 2016 to 2021 revealed clusters characterized as high-high, low-low, high-low and low-high. Specifically, high-high clusters were identified in Guangxi, Hunan, Jiangxi, and Guangdong provinces; low-low in Xinjiang Uygur Autonomous Region and Inner Mongolia Autonomous Region; high-low in Sichuan province; and low-high in Fujian and Anhui provinces.ConclusionsFrom 2016 to 2021, there was evident spatial clustering of health personnel and various health technical personnel in Chinese maternal and child healthcare institutions, indicating regional imbalances.

Childhood trauma associated with psychotic-like experiences among people living with HIV: The chain mediation effect of stigma and resilience

BackgroundPrevious research established the associations between childhood trauma and psychosis, but the effects of childhood trauma on psychotic-like experiences (PLEs) among people living with HIV (PLWH) and the potential mediation mechanisms in these associations remain unclear. ObjectiveThis study aimed to investigate the effects of childhood trauma on PLEs, as well as the chain mediation roles of stigma and resilience in this relationship. Furthermore, we explored whether the aforementioned associations differed when hallucinatory experiences (HEs) and delusional experiences (DEs) were separately modeled. Participants and settingThe sample included 333 outpatients participants (95.2 % males, Mage = 28.24 ± 7.12) living with HIV recruited from Hunan Province, China; and data were collected with a cross-sectional survey. MethodThe hypothesized chain mediation models were examined using SPSS PROCESS macro 3.3 software. ResultsVarious influencing mechanisms of childhood trauma on HEs and DEs were examined in this study. Our results showed that, (a) childhood trauma directly exerted negative effect on HEs, while the chain mediation effect of stigma and resilience were not statistically significant. Conversely, (b) childhood trauma exerted no direct influence on DEs but rather through the chain mediation effect of stigma and resilience. ConclusionsThe identification of two different routes between that childhood trauma can have on HEs and DEs highlighted the importance of tailored prevention and intervention among PLWH with a history of childhood trauma.