BackgroundInsertion/deletion polymorphism (InDel) markers, mainly characterized by diallelic length polymorphisms, have been extensively investigated in forensic applications. The Salar ethnic group is one of the ten officially recognized Muslim minorities in China, with a large population mainly residing in Qinghai Province, China, yet there have been few population genetics studies on the Salar ethnic group, let alone those using InDel markers. AimThe present study aims to explore the genetic traits and enrich the genetic resources of the Qinghai Salar ethnic group through a validated InDel panel developed in-house. Subjects and methodsForensic performance of the novel self-constructed 43-InDel panel, designed for individual identification, was evaluated in the Qinghai Salar ethnic group from China. In addition, based on these 43 InDels, the genetic characteristics of the Qinghai Salar ethnic group were systematically explored by incorporating 27 reference populations from five continents. ResultsThe results indicated that most loci in the 43-InDel panel were genetically polymorphic in the Qinghai Salar ethnic group, and the novel panel could serve as an efficient tool for individual identification of the studied group, with a combined discrimination power of more than 0.9999. Population genetics analyses demonstrated that the Qinghai Salar ethnic group was genetically closer to East Asian populations than to other continental populations, especially the Hui ethnic group in Northwest China and Han Chinese in Beijing. ConclusionIn summary, the in-house developed 43-InDel panel could be an effective tool for individual identification and provide additional genetic information for the Qinghai Salar ethnic group.
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