Horse Serum Cholinesterase Research Articles

Anticholinesterase properties of certain o-ethyl-s-alkylmethylthiophosphinates Communication 2. Kinetics of the inhibition of cholinesterase and acetylcholinesterase by O-ethyl-S-n-alkylmethylthiophosphinates

1. The kinetics of the interaction of O-ethyl-S-n-alkylmethylthiophosphinates with horse serum cholinesterase and ox erythrocyte acetylcholinesterase were investigated. The constants of the aqueous alkaline hydrolysis of the compounds studied were determined. 2. The anticholinesterase activity of O-ethyl-S-n-alkylmethylthiophosphinates is greatly dependent upon the length of the alkyl radical.

ELECTROPHORETIC MOBILITY OF NATICE AND NEURAMINIDASE-TREATED HORSE-SERUM CHOLINESTERASE.

ELECTROPHORETIC MOBILITY OF NATICE AND NEURAMINIDASE-TREATED HORSE-SERUM CHOLINESTERASE.

Treatment of Horse Serum Cholinesterase with Sialidase.

Treatment of Horse Serum Cholinesterase with Sialidase.

A new type Warburg vessel and its application to the study of some kinetics of cholinesterase inhibition

A reaction vessel of a new type has been designed for use with the Warburg apparatus. The vessel is particularly suitable for determining rates of cholinesterase inhibition in vitro at short time intervals after mixing. The method has been used to obtain the rate constants for the in vitro inhibition of horse serum cholinesterase and bovine erythrocyte cholinesterase by isopropyl methylphosphonofluoridate, ethyl N,N-dimethylphosphoramidocyanidate and O, O-diethyl-S-ethyl-2-mercaptoethyl phosphorothiolate. The activation energy for the inhibition reaction with O,O-diethyl-S-ethyl-2-mercaptoethyl phosphorothiolate has been determined.

Simultaneous verification by paper chromatography and Warburg's method of the hydrolysis of succinylcholine by cholinesterase

Succinyl monocholine ester has been identified by paper chromatography as an intermediate in the breakdown of succinylcholine in the presence of horse serum cholinesterase.