The distribution of the broad-snouted caiman is concentrated in the Brazilian coastal area, the area with the highest urbanization rate in the country. The anthropic pressure makes constant monitoring a necessary effort. Aiming to conserve the species and expand genetic information of Caiman latirostris, we analyzed the genetic diversity and structure of a population in Serra – ES. We compared it with analyzed data of populations distributed in Brazil. We used ten microsatellite markers from 39 samples collected from five lakes in the industrial complex of Serra – ES and data obtained from the literature with information from the same genetic markers for comparative analysis. We observed that the population of ES is genetically isolated from the other nine Brazilian populations. There are high genetic similarities among the lakes, probably due to the low geographic distance (between 100 m and 3 km) among them. The mean heterozygosity observed for the ES population was 0.434, indicating that this population has lower genetic diversity than the others already studied around Brazil (average of 0.54) and a high inbreeding index (0.271) probably related to the isolation of the population.
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