High Ratio Research Articles

On the Use of Strong Proton Donors as a Tool for Overcoming Line Broadening in NMR: A Comment.

Overcoming line broadening of labile protons and achieving high-resolution NMR spectra is crucial for the structural and conformational analysis of organic molecules. Recently, Ma etal. (Magn. Reson. Chem. 2024, 62, 198-207) demonstrated the effectiveness of 2,2,2-trifluoroacetic acid (TFA) in sharpening NMR signals for nitrogen-containing compounds which exhibit prototropic tautomerization or conformational isomerism using high molar ratio of [acids]/[solute] ~ 5 to 200. In this commentary, we provide an overview of earlier publications and highlight the extensive applications of TFA in enhancing NMR resolution across a variety of organic functional groups, with the use of very small ratios of [acids]/[solute] ~ 10-3 to 10-2. The prospects for the unequivocal structure analysis using labile protons as the starting point will be analyzed.

New particle formation from isoprene under upper-tropospheric conditions

Aircraft observations have revealed ubiquitous new particle formation in the tropical upper troposphere over the Amazon1,2 and the Atlantic and Pacific oceans3,4. Although the vapours involved remain unknown, recent satellite observations have revealed surprisingly high night-time isoprene mixing ratios of up to 1 part per billion by volume (ppbv) in the tropical upper troposphere5. Here, in experiments performed with the CERN CLOUD (Cosmics Leaving Outdoor Droplets) chamber, we report new particle formation initiated by the reaction of hydroxyl radicals with isoprene at upper-tropospheric temperatures of −30 °C and −50 °C. We find that isoprene-oxygenated organic molecules (IP-OOM) nucleate at concentrations found in the upper troposphere, without requiring any more vapours. Moreover, the nucleation rates are enhanced 100-fold by extremely low concentrations of sulfuric acid or iodine oxoacids above 105 cm−3, reaching rates around 30 cm−3 s−1 at acid concentrations of 106 cm−3. Our measurements show that nucleation involves sequential addition of IP-OOM, together with zero or one acid molecule in the embryonic molecular clusters. IP-OOM also drive rapid particle growth at 3–60 nm h−1. We find that rapid nucleation and growth rates persist in the presence of NOx at upper-tropospheric concentrations from lightning. Our laboratory measurements show that isoprene emitted by rainforests may drive rapid new particle formation in extensive regions of the tropical upper troposphere1,2, resulting in tens of thousands of particles per cubic centimetre.

Magmatic evolution and magma chamber conditions of the Alpehué tephra from Sollipulli Volcano, Andean Southern Volcanic Zone, Chile/Argentina

The trachydacitic Alpehué tephra from Sollipulli volcano (Andean Southern Volcanic Zone), consists of ignimbrite and fallout from a Plinian eruption about 3000 years ago. It is mainly composed of (1) crystal-rich pumice and ash but also contains (2) chilled knobbly basaltic lava clasts and (3) mostly highly inflated glomerocrystic fragments with high crystal-glass ratios interpreted to represent a crystal mush zoned from basaltic to dacitic bulk compositions. Knobbly lava clasts are of three types: (a) a very phenocryst-poor basalt, (b) a basalt with large, unzoned olivine and plagioclase phenocrysts and glomerocrysts, and (c) mixtures of microcrystalline basalt with various fragments, glomerocrysts and crystals derived from a crystal mush. Clast type (4) in the tephra is banded pumices in which the three magmatic components occur variably mingled. Thermobarometry and petrographic observations, particularly presence or absence of amphibole, constrain an upper-crustal succession of a lower basaltic reservoir, a zoned basaltic to dacitic crystal mush reservoir, and a separate trachydacite magma chamber on top. All Alpehué magmatic components form a coherent liquid line of descent which supports the interpretation that the crystal mush reservoir is a gradually solidifying magma chamber, not the result of large-scale crystal-liquid segregation. The trachydacite magma may originally have formed as melt escaping from the crystal-mush reservoir but subsequently underwent a long and complex evolution recorded in large strongly zoned plagioclase phenocrysts including resorption horizons. The ascending mafic magmas collected samples from the crystal mush body and intruded the trachydacite reservoir. The phenocryst-poor basalt (a) arrived first and entrained and partially resorbed plagioclase from the host magma. The phyric basalt (b) arrived later and did not resorb entrained plagioclase before eruption. Estimated cooling times, plagioclase resorption times and ascent rates avoiding amphibole breakdown limit the duration of these pre-eruptive processes to not more than a few days.

A new look at disk winds and external photoevaporation in the σ-Orionis cluster

Disk winds play a crucial role in the evolution of protoplanetary disks. Typical conditions for star and planet formation are in regions with intermediate or strong UV radiation fields produced by massive stars. In these environments, internally or externally driven winds can occur. The is the ideal site to study disk winds under these conditions; its outer parts, exposed only to mild UV fields, can be used to study disk evolution, while its innermost regions can be used to study the effect of external irradiation. Our goal is to study disk winds in the by looking at the properties of optical forbidden lines, and comparing them with other star-forming regions at different ages, to search for potential signatures of disk evolution and external photoevaporation. We analyzed the and 6716 lines using high-resolution MIKE spectra for a sample of 27 classical T Tauri stars and complemented by intermediate-resolution X-shooter data. We decomposed the line profiles into multiple Gaussian components. We calculated luminosities, line ratios, and kinematic properties of these components. We find that the luminosity and kinematic properties for our are similar to those found in low-mass star-forming regions. The frequency of single-component profiles reflects the expected evolutionary stage given the intermediate age of 3-5 Myrs). This points to internal processes contributing to the line emission. However, the highly irradiated disks in the cluster do not follow the accretion luminosity- line luminosity relation found in low-mass star-forming regions, and all exhibit single-component line profiles. Line ratios of highly ionized species of NII and SII show higher ratios than typical values found in sources in low-mass star-forming regions. These are interpreted as signatures of external photoevaporation. We show the potential of using multiple forbidden emission lines to study both internally and externally driven disk winds. In the case of the innermost regions are clearly affected by external irradiation, as evidenced by the lack of correlation in the OI luminosity relation. The broad line widths of close-in sources, however, indicate a possible contribution from internal processes, such as magnetohydrodynamical winds and/or internal photoevaporation. This suggests a coevolution of internal and external winds in the while pointing toward a new way to disentangle these processes.

Surface-Grinding-Induced Recrystallization and Metal Flow Causes Corrosion-Assisted Penetrating Attack of High-Mn–Low-CR Casting Steel in Humid Environments

This study examined the surface-grinding-induced microstructural modifications and corrosion attacks in a penetrating form of a high-Mn–low-Cr casting steel slab under humid environments. Various experimental and analytical findings from field-emission scanning electron microscopy, electron backscatter diffraction, transmission electron microscopy, and electrochemical analyses revealed that the abrasive grinding process led to the formation of a surface deformed region, comprising a recrystallized fine grain layer and multiple streamlines. Corrosion initially occurs preferentially along the boundary areas where Cr(Mn)23C6 particles are precipitated. Moreover, the corrosion products (Fe-based oxy/hydroxides) with a high volumetric expansion ratio detach readily from the surface deformed regions, facilitating the easy penetration of corrosive media. In contrast to conventional low-alloyed steels, which exhibit uniform corrosion behavior, corrosion-assisted penetrating attacks on ground high-Mn–low-Cr casting steel slabs occur more severely and frequently during the summer/dry season (i.e., relative humidity levels around 60% to 80%, rather than 100%) when a thin water film can form on the steel surface. Based on the result, effective technical strategies in terms of metallurgical and environmental aspects to mitigate the risk of corrosion-assisted penetrating attack of high-Mn–low-Cr casting steel were discussed.

Aerodynamic performance enhancement of a vertical-axis wind turbine by a biomimetic flap.

We improve the aerodynamic performance of a simplified vertical-axis wind turbine (VAWT) using a biomimetic flap, inspired by the movement of secondary feathers of a bird's wing at landing (Liebe 1979). The VAWT considered has three NACA0018 straight blades at the Reynolds number of 80000 based on the turbine diameter and free-stream velocity. The biomimetic flap is made of a rigid rectangular curved plate, and its streamwise length is 0.2cand axial (spanwise) length is the same as that of blade, wherecis the blade chord length. This device is installed on the inner surface of each blade. Its one side is attached near the blade leading edge (pivot point), and the other side automatically rotates around the pivot point (without external power input) in response to the surrounding flow field during blade rotation. The flap increases the time-averaged power coefficient by 88% at the tip-speed ratio of 0.8, when its pivot point is at 0.1cdownstream from the blade leading edge. While the torque on the blade itself does not change even in the presence of the flap, the flap itself generates additional torque, thus increasing the overall power coefficient. The phase analysis indicates that the power coefficient of VAWT significantly increases during flap opening to full deployment through the interaction with vortices separated from the blade leading edge. When the pivot point of flap is farther downstream from the leading edge or the flap operates at a high tip-speed ratio, the performance of the flap diminishes due to its weaker interaction with the separating vortices.

Breaking the angular dispersion limit in thin film optics by ultra-strong light-matter coupling

Thin film interference is integral to modern photonics, e.g., allowing for precise design of high performance optical filters, photovoltaics and light-emitting devices. However, interference inevitably leads to a generally undesired change of spectral characteristics with angle. Here, we introduce a strategy to overcome this fundamental limit in optics by utilizing and tuning the exciton-polariton modes arising in ultra-strongly coupled microcavities. We demonstrate optical filters with narrow pass bands that shift by less than their half width (< 15 nm) even at extreme angles. By expanding this strategy to strong coupling with the photonic sidebands of dielectric multilayer stacks, we also obtain filters with high extinction ratios and up to 98% peak transmission. Finally, we apply this approach in flexible filters, organic photodiodes, and polarization-sensitive filtering. These results illustrate how strong coupling provides additional degrees of freedom in thin film optics that will enable exciting new applications in micro-optics, sensing, and biophotonics.

Tuning the interaction between Cu and surface -OH in Cu/Ti3C2 MXene derived catalyst for enhanced semi-hydrogenation of dimethyl oxalate

Methyl glycolate (MG), a crucial chemical raw material and fine chemical intermediate, holds immense potential in the production of the next-generation biodegradable material, polyglycolic acid, via polycondensation reactions. In this study, layered Ti3C2 MXene was used as support precursor to construct Cu based catalysts for the semi-hydrogenation of dimethyl oxalate to MG. By fine-tuning the loading method of Cu species, the interaction between Cu species and surface hydroxyl groups (-OH) was modulated, and the effect on the semi-hydrogenation performance of Cu-based catalysts were thereby explored. These catalysts underwent rigorous characterization using BET, SEM-EDS, XRD, FT-IR, H2-TPR and XPS techniques. The results revealed that varying loading methods significantly influenced the interaction between Cu species and surface -OH on Ti3C2 MXene during preparation process, altering the content of Ti-O-Cu as well as the Cu0 ratio of Cu/Ti3C2-derived catalysts. The Cu/Ti3C2 MXene-I catalyst displayed remarkable performance, achieving a MG selectivity of 88.85 % and stability of up to 120 h in the DMO hydrogenation reaction due to its high Cu0 ratio and large Cu particles.

Geometric Optimization of the Five-Point Double-Toggle System for the Clamping Unit of an Injection Molding Machine with the Response Surface Method

Injection molding is one of the most used processes for the manufacture of plastic products with high-volume capacity. The five-point double toggle mechanism is frequently employed in the clamping unit to augment the force generated by a linear actuator, thereby achieving the requisite clamping force. This is a crucial aspect that determines the productivity and quality of the finished product in the injection molding process. Nevertheless, the geometrical synthesis of this type of mechanism has not been sufficiently addressed with regard to its integration into the mold design and the opening/closing stroke. This paper presents a novel approach for analyzing and evaluating the impact of parameters pertaining to mechanism posture on its force amplification, employing the Taguchi method. The relationship between force amplification ratio and the most influential parameters is simplified with the Face-Centered Central Composite Design (FCCCD) method, thereby allowing the optimal posture of the mechanism at the mold-closing stage to be determined. With these optimal parameters, once the mold height and desired opening/closing stroke have been selected, the dimensions of the links in the mechanism can be calculated. The results demonstrate that there are numerous combinations of these parameters that can yield a high force amplification ratio, thus providing the designer with a range of options for the design of a clamping unit. The proposed method can be employed at the initial stage of the design process to obtain a preliminary design, thus preparing for the dynamic analysis or further optimization problems of the mechanism.

Trial Design of a Truss Bridge Prefabricated Using a Rectangular Steel Tube—Ultra-High-Performance Concrete Composite

In order to promote the development of bridge assembly technology and accelerate the application of rectangular steel-tube–concrete composite truss bridges, this study focuses on the Yellow River Diversion Jiqing Main Canal Bridge as the engineering example and conducts a numerical analysis of a rectangular steel-tube–concrete composite truss bridge. Based on the results of the analysis, structural optimization is achieved in three dimensions—structural design, construction methods, and force analysis—leading to the establishment of key design parameters for through-type ultra-high-performance rectangular steel-tube–concrete composite truss bridges. The results show that filling the hollow sections with ultra-high-strength concrete can significantly enhance the load-bearing capacity. Additionally, employing prestressed concrete components addresses the bending and tensile load capacity challenges of composite structures, thus maximizing the material strength advantages. The proposed preliminary design scheme incorporates prestressed PBL-reinforced tie rods filled with ultra-high-performance concrete with optimal design parameters, such as high span ratios, wide span ratios, and ideal segment lengths, are suggested to ensure that the strength, stiffness, and stability comply with relevant standards. While ensuring that the structure meets safety, applicability, and durability criteria, the preliminary design scheme reduces steel usage by 23.5%, concrete usage by 11.6%, and overall costs by 17.29% compared to the original design. The proposed design demonstrates distinct advantages over the original in terms of mechanical performance, construction efficiency, economic viability, and durability, highlighting its promising application potential.

Buprenorphine Salivary Gland Accumulation Sustaining High Oral Fluid Exposure and Increasing the Risk of Streptococcus mutans Biofilm Formation.

The US Food and Drug Administration (FDA) issued a warning about buprenorphine-induced dental caries of unknown mechanism in 2022. To investigate the potential mechanism, the association between local buprenorphine exposure and dental biofilm formation will be explored in this study. Female F344 rats were dosed with sublingual buprenorphine film or intravenous injection to explore the oral cavity exposure of the buprenorphine. The buprenorphine distribution in salivary glands after the sublingual and intravenous administration was also evaluated. To investigate the effects of buprenorphine exposure on dental caries formation, buprenorphine's impact on the biofilm formation of S. mutans in vitro was measured. The absolute sublingual bioavailability of buprenorphine in rats was 17.8% with a high ratio of oral fluid exposure to blood concentration in the pharmacokinetic study. Salivary gland concentrations of buprenorphine and its active metabolite norbuprenorphine were significantly higher than their blood concentrations after both sublingual (s.l.) and intravenous (i.v.) administration. Correlation analysis showed that the oral fluid concentration of buprenorphine and norbuprenorphine was highly correlated to salivary gland concentration rather than blood concentration. These data indicate that the salivary gland serves as an accumulation organ for buprenorphine, allowing prolonged oral fluid exposure to buprenorphine. Lastly, buprenorphine and its metabolites contributed to the biofilm formation of S. mutans in high concentration. Sublingual administration substantially increased the salivary gland distribution of buprenorphine and norbuprenorphine. Depot effects following sublingual dosing and salivary gland accumulation likely sustained high oral fluid exposure to buprenorphine and stimulated the biofilm formation of S. mutans.

All-solid-waste cementitious materials for grouting: Effects of alkali content and elemental ratios on performance and sustainability

The use of all-solid waste cementitious materials (ACM) in coal mine grouting backfill offers substantial green, low-carbon, and energy-saving benefits. This study systematically examines the effects of combining carbide slag (CS), fly ash (FA), and ground granulated blast furnace slag (GGBS) on the strength, workability, hydration characteristics, and microstructure of ACM. The utilization of sulfur-containing CS was achieved. The detrimental effects of delayed FA reaction and prolonged setting time can be mitigated by the introduction of high alkalinity or an increase in the Ca/Si ratio. The compressive strength may also be enhanced. The addition of excessive alkalinity (10 %) will result in a prolongation of the setting time. The primary hydration products include calcium aluminum silicate hydrate and magnesium-aluminum layered double hydroxide. Low Ca/Si ratios favor alkali metal ion charge balance, facilitating the transformation of silicate gels from single to double chains, while excessively high ratios reduce polymerization. Gmelinite forms when the (Ca+Na)/(Al+Si) ratio exceeds 1.6, and high NaOH concentrations inhibit ettringite formation. Validation shows that a GGBS:FA:CS= 3:1:3 mix with 4 % alkali binder (Group A2) meets mine grouting backfill criteria, with 80 % lower carbon emissions, 68 % lower energy intensity, and 50 % lower cost than cement. This research offers a viable pathway for the comprehensive utilization of multi-solid waste in mining applications.

Dietary plant-to-animal protein ratio and risk of cardiovascular disease in 3 prospective cohorts

BackgroundDietary guidelines recommend substituting animal protein with plant protein, however, the ideal ratio of plant-to-animal protein (P:A) remains unknown. ObjectivesWe aimed to evaluate associations between the P:A ratio and incident cardiovascular disease (CVD), coronary artery disease (CAD), and stroke in 3 cohorts. MethodsMultivariable-adjusted Cox proportional hazard models were used to estimate hazard ratios (HRs) for CVD outcomes among 70,918 females in the Nurses’ Health Study (NHS) (1984–2016), 89,205 females in the NHSII (1991–2017) and 42,740 males from the Health Professionals Follow-up Study (1986–2016). The P:A ratio was based on percent energy from plant and animal protein and assessed using food frequency questionnaires every 4 y. ResultsDuring 30 y of follow-up, 16,118 incident CVD cases occurred. In the pooled multivariable-adjusted models, participants had a lower risk of total CVD [HR: 0.81; 95% confidence interval (CI): 0.76, 0.87; P trend < 0.001], CAD (HR: 0.73; 95% CI: 0.67, 0.79; P trend < 0.001), but not stroke (HR: 0.98; 95% CI: 0.88, 1.09; P trend = 0.71), when comparing highest to lowest deciles of the P:A ratio (ratio: ∼0.76 compared with ∼0.24). Dose–response analyses showed evidence of linear and nonlinear relationships for CVD and CAD, with more marked risk reductions early in the dose-response curve. Lower risk of CVD (HR: 0.72; 95% CI: 0.64, 0.82) and CAD (HR: 0.64; 95% CI: 0.55, 0.75) were also observed with higher ratios and protein density (20.8% energy) combined. Substitution analyses indicated that replacing red and processed meat with several plant protein sources showed the greatest cardiovascular benefit. ConclusionsIn cohort studies of United States adults, a higher plant-to-animal protein ratio is associated with lower risks of CVD and CAD, but not stroke. Furthermore, a higher ratio combined with higher protein density showed the most cardiovascular benefit.

A Design Strategy for Low-Cost Single/Dual-Band Photodetector: Bulk Heterojunction and Interface Engineering.

Photodetectors (PDs) with band selection functions are highly desirable for the future complex environment. Currently, band-selective PDs are typically realized by integrating various optical filters with broadband PDs or constructing heterojunction with multi-photo-absorbing layers. However, these methods cause increased manufacturing costs and device integration complexity. Here, a novel solution-processed perovskite bulk heterojunction (BHJ) layer (MAPbI3:MA3Bi2I9) is developed as photo-absorbing layer, and an effective interface engineering is proposed to selectively shield photo-generated carriers in the BHJ. By regulating the bias voltage, the tunneling probability of photo-generated carrier of MAPbI3 in the BHJ layer can be easily controlled, enabling band-selective capability. The PD exhibits a superior photo-response in the visible and near-infrared region at the -0.3V, with responsivity of 26.5 and 70.2mAW-1 for 500 and 740nm, respectively. But, at the 0.1V, the PD responds only to the visible region with a photo-response of 3.7mAW-1 (500nm) and maintains a high rejection ratio (R500 nm/R740 nm) of 28.5. This PD also exhibits a fast response speed (rise time: 220µs, fall time: 240µs). This work provides a novel strategy to fabricate a cost-effective multifunctional photodetector for future advanced optoelectronic system.

Nanochannel geometry-depended behaviors on ion selectivity for salinity gradient energy harvesting

Nanofluidic osmotic energy, which can be directly converted into electricity, is considered a clean and sustainable energy that effectively utilizes salinity gradients. The rational construction of nanochannel is of great significance to ion transport and osmotic energy conversion, but there is currently little attention paid to naturally formed rough and irregular channels. In this study, a model that considers the effects of nanochannel cone angle and waveform surface on interface reaction coupling was established for osmotic energy conversion. The results indicate that cone angle and waveform have a significant effect on osmotic energy conversion. It is found that the reduction of cone angle and the addition of waveform will improve ion selectivity and increase energy conversion efficiency, and ion rectification effect of corrugated cylindrical channel is the most obvious. Meanwhile, enlarging waveform dimensions leads to a significant overlap of electric double layer, resulting in a growth in cation transference number and selectivity, thereby enhancing the system's energy conversion efficiency, which can reach 49.62%. At low concentration ratios, the waveform dimensions are inversely proportional to the maximum output power, whereas at high concentration ratios, increasing the waveform dimensions and applying the waveform at channel entrance can efficiently improve the maximum output power.

Homogeneous Crystallization of Nickel as Ni₃S₄ in a Fluidized Bed Reactor with Supersaturation Control

This study investigates the recovery of nickel sulfide (NiS) using fluidized bed homogeneous crystallization (FBHC), emphasizing the process from nucleation to crystallization. Sludge production in conventional chemical precipitation becomes a drawback in treating wastewater economically and efficiently. FBHC offers a promising alternative by minimizing sludge generation. The study examines various operational parameters, including hydraulic retention times (HRT), reflux ratio (R), initial nickel concentration, and static bed height (SBH), to optimize NiS recovery. Results show that FBHC achieves high total removal efficiency (TR) and crystallization ratio (CR), under the optimum condition of HRT = 15min, R = 8.3, initial nickel concentration = 147mg/L, and SBH = 35cm. The agglomeration of Ni(OH)2 was found to be crucial for the crystal growth during the surface oxidation of Ni3S4. This technological approach to wastewater treatment offers highly efficient heavy metal removal from wastewater.

An Impact Inertial Piezoelectric Actuator With High Thrust-Weight Ratio Driven by Coupling the Inertial Force and Friction Force

An Impact Inertial Piezoelectric Actuator With High Thrust-Weight Ratio Driven by Coupling the Inertial Force and Friction Force

Effect of pore plugging on transpiration cooling performance implemented with perforated flat plates

Transpiration cooling is expected to be one of the most effective cooling technologies for gas turbine blades. However, dust deposition can easily lead to pore plugging, which limits its practical application in engineering. The plugging of pores in conventional porous media models is difficult to predict, so it is essential to construct computational models with finely controllable porous structures. In this study, a transpiration cooling configuration with straight holes perforated with a diameter of 0.5 mm was adopted. The effects of plugging ratio and region on flow and heat transfer under different coolant injection ratios and pump power conditions were investigated by numerical simulation. The results showed that at constant mass flow rate, the overall cooling performance increased as the plugging ratio increased at low injection ratios. At high injection ratios, the opposite was true. With a certain pump power, as the plugging rate increased, the flow loss coefficient rose, leading to a decrease in cooling efficiency. Transpiration cooling efficiency was also highly sensitive to the distribution of plugged holes at the same plugging ratio. When the coolant flow was sufficient, the farther the plugged area was from the leading edge, the smaller the reduction in cooling efficiency.

Laser-engraved holograms as entropy source for random number generators

Our study introduces a novel approach to true random number generation (TRNG) using speckle patterns generated by laser-engraved holograms on carbon fiber-reinforced polymer (CFRP) composite substrates. Unlike previous methods, our approach simplifies the process by generating the necessary image dataset from a single microscope image of the engraved hologram. We achieve a high extraction ratio of 76 %, demonstrating the effectiveness of our TRNG. Moreover, our method successfully passes rigorous statistical tests proposed by the National Institute of Standards and Technology (NIST), indicating its suitability for cryptographic and secure system applications. This work offers promising implications for enhancing security in various domains, from secure communication networks to IoT devices.

Efficient Development of Nanobody-Based Affinity Chromatography for AAV8 Purification

Adeno-associated virus serotype 8 (AAV8) is a highly effective vector for gene therapy. However, its purification remains challenging due to its low natural abundance and stringent purity requirements. This study aimed to develop an affinity chromatography resin utilizing nanobodies (Nbs) to enhance AAV8 purification efficiency. An AAV8-specific Nb library was constructed, leading to the identification of Nb9 as the most promising candidate based on its high binding affinity, stability and yield. Nb9 was expressed in Pichia pastoris, resulting in high yield and exceptional purity. Two types of agarose resins, Epoxy activated Bestarose 6B and PabPur SulfoLink Beads 4FF, were employed for Nb9 conjugation. Epoxy activated Bestarose 6B resin exhibited a significantly higher ligand density (9.12 mg/mL). Binding capacity assessments of the LQ01 resin demonstrated optimal performance at pH 7.0, with diminishing efficacy at lower and higher pH levels. Different NaCl concentrations influenced the binding efficiency, providing critical insights for refining purification conditions. Purification trials exhibited high specificity, purity and consistent VP protein ratio, as evidenced by SDS-PAGE analysis, confirming effective AAV8 capture and elution. Furthermore, the resin demonstrated robust performance across repeated cycles, retaining 71.9% of its initial binding capacity after 20 uses and maintaining stability with only a 6% reduction after 7 days at 37°C. These findings highlight LQ01's potential for scalable and cost-effective AAV8 purification, while demonstrating the broader applicability of Nbs in affinity chromatography and biotechnological processes.