An online rapid screening HPLC system establishing and applying discovered a new type of natural chemical ligand of the BRD4-BD1 from Hedyotis diffusa.

EuFe-MOF exhibiting excellent peroxidase-like activity and red fluorescence for colorimetric/fluorescence dual-modal sensor to detect xanthine in aquatic products.

A validated HPLC method for kallikrein activity assessment and its use in evaluating fruit extracts.

Determination of four nitrosamines in cosmetics using PDMS-coated rotating disk-assisted supercritical fluid extraction coupled with high performance liquid chromatography.

Thermal degradation kinetics of curcumin in dry-heated turmeric powder studied by front-face synchronous fluorescence spectroscopy.

Modified Shenlingbaizhu decoction suppresses colon cancer via enhancing memory-like Tfh differentiation and B cell responses.

Dendritic mixed-mode stationary phases prepared by thiol-epoxy click reaction for the determination of bisphenols in a variety of complex samples.

Encapsulation of hydroxyurea in exosomes derived from adipose mesenchymal stem cells enhances anticancer efficacy in breast cancer.

Peucedanum praeruptorum Dunn leaves extract and its active coumarin praeroside Ⅴ alleviate ethanol-induced acute duodenal damage by suppressing apoptosis and ferroptosis in mice.

Sulforaphane-rich aqueous broccoli seed extract suppresses diet-induced obesity via 5-HT2A/AMPK signaling in mice.

Development of a reference material for the accurate determination of multiple pesticide residues.

Research progress on enantiomers detection in fermented foods.

Tetrodotoxin in diet items of highly toxic ribbon worm Cephalothrix cf. simula (Palaeonemertea, Nemertea).

Phenanthrene sorption in expanded polystyrene microplastics and environmental aging effects: A Venezuelan caribbean beaches case study.

Effective myocardial protection during cardiac surgery is essential, yet the differential metabolic stress of the left (LV) and right ventricles (RV) to ischemia-reperfusion remains poorly characterized. This predefined substudy of a multicenter randomized trial conducted in the United Kingdom (ISRCTN33084113) evaluated remote ischemic preconditioning in patients undergoing coronary artery bypass grafting (CABG) or aortic valve replacement (AVR). Paired LV and RV biopsies were obtained before ischemia and after reperfusion. High-performance liquid chromatography was used to quantify metabolite concentrations and calculate markers of energetics. Cross-clamp time and postoperative troponin levels were recorded. Eighty-nine patients were included (CABG: n=49; AVR: n=40). Following ischemia-reperfusion, LV exhibited greater decline in metabolites than the RV. In CABG, this included larger sustained fall in ATP (-0.90 vs -0.13μmol/mg, p=0.0032) and glutamate (-4.22 vs -1.33μmol/mg, p=0.0009), alongside ADP (-0.53 vs -0.20μmol/mg, p=0.0196), GTP (-0.04 vs -0.01μmol/mg, p=0.0160), and B-NAD (-0.15 vs -0.04μmol/mg, p=0.0137). Computed energy charge decreased significantly in both ventricles (LV: p=0.0002; RV: p=0.010), but remained stable in AVR. ATP/AMP and ATP/ADP ratios declined more in the LV, suggesting impaired energy buffering. There was no significant correlation between cross-clamp time and changes in energy charge in either the LV or RV. Troponin release correlated with ischemic time in CABG, but EC decline did not. The LV shows greater depletion of myocardial metabolites than the RV following ischemia-reperfusion, particularly in CABG patients. These findings support ventricle-specific strategies to optimize myocardial protection.

Colorimetric aptasensor based on GO-SELEX screened aptamers for detecting norfloxacin antibiotic residues in fish and shrimp.

Eucommia ulmoides leaf as an alternative substrate for kombucha fermentation: the role of microbial communities in dynamics and metabolic profile.

Core-shell magnetic molecularly imprinted microspheres for selective extraction and determination of Schisandrol A with hepatoprotective evaluation in zebrafish larvae.

Zhi-Chuan-Ling alleviates OVA-induced allergic asthma by suppressing M2 macrophage polarization via the PI3K/AKT/mTOR/STAT6 pathway.

Gastrointestinal endoparasitic diseases, particularly those caused by some strongyles, represent one of the main health challenges in equine production. The prolonged use of synthetic anthelmintics has led to the development of resistance, which has prompted the search for more sustainable therapeutic alternatives. However, plant extracts rich in secondary metabolites are emerging as a promising option. The objective of this study was to evaluate the in vitro anthelmintic activity of aqueous extracts and bioactive compounds (total phenols and saponins) from Azadirachta indica and Cnidoscolus angustidens against L3 larvae of strongyles in horses. Three extracts were prepared: the first one from A. indica, the second one from C. angustidens, and the third one as a 1:1 (v/v) mixture thereof. These were characterized phytochemically by spectrophotometry and High-performance liquid chromatography (HPLC). Anthelmintic activity was evaluated by a larval inhibition assay in ELISA plates, using two doses (1200 and 1800 µL). A. indica had the highest concentration of total phenols and saponins, while C. angustidens stood out for its tannin content and total phenols. The highest larval inhibition was recorded with the total phenols of C. angustidens, the saponins of A. indica, and crude extracts of both species. In contrast, the mixture of extracts showed a significant reduction in anthelmintic activity, evidencing a possible antagonistic interaction between their metabolites. A potential anthelmintic activity of A. indica and C. angustidens aqueous extracts, as natural sources of anthelmintic compounds, was detected against L3 larvae of strongyles in equines.