Higher-order Multiples Research Articles

Incidence of Monozygotic Multiple Pregnancies After Single Embryo Transfer (SET): Review of SART Reported Outcomes [37K

INTRODUCTION: To calculate the incidence and risk factors of monozygotic multiple pregnancies after SET. METHODS: A review of all SET cycles from 2005-2015 that resulted in more than one fetal heartbeat on ultrasound was conducted. Descriptive statistics were used to determine factors associated with an increased risk of multiple pregnancies after SET. RESULTS: 67,884 SET cycles met inclusion criteria, resulting in 1,307 (1.9%) twin gestations and 36 (0.06%) triplet or higher-order multiples. Mean age and BMI of subjects was 35.7±5.4 years and 24.6±14.9 kg/m2, respectively. Majority of subjects were from the Northeast or from the southern U.S. Autologous oocytes accounted for 84.2% of the twin cases and 91.6% of the triplets or more. 63% of the twins and 72% of the triplets or more underwent a fresh cycle. Sperm source was from the partner in 96% of both twin and triplet cohorts. CONCLUSION: Risk of monozygotic twinning (MZT) in IVF is 1.9% which is 5 times the rate of MZT in natural conception. While the risk of identical triplets is low (0.06%), it is not negligible. The mechanism underlying this increased risk in IVF embryos is unclear and probably includes culture conditions as well as micromanipulation. It is unclear how a single embryo splits into three: Is it the result of two sequential monozygotic splitting? Prospective IVF patients should be informed of the risk of identical multiples even with SET. Patients who desire the transfer of two blastocyst should be warned of at least one embryo splitting, ending up with a triplet pregnancy.

Choosing Wisely: Bedrest-A Panacea for All That Ails the Gravida?

Bedrest has been frequently prescribed for various high-risk conditions during pregnancy. A common indication cited is concern regarding increased risk of spontaneous preterm birth, known to be associated with gestations with twins or higher-order multiples, polyhydramnios, presence of preterm contractions with or without evidence of cervical change, sonographic cervical shortening, presence of a dilated cervix with bulging membranes, or preterm premature rupture of membranes. In some cases, there is concern that excessive movement may increase the risk of antepartum hemorrhage, as may be with placenta previa, with or without a higher risk of a morbidly adherent placenta or a vasa previa. It is also thought that improved splanchnic perfusion with bedrest may enhance blood supply to the uterus with possible benefit in conditions such as preeclampsia (or gestational hypertension) or fetal growth restriction. However, there is no evidence of any benefit of bedrest or reduced activity during pregnancy for either of these conditions. On the contrary, there is evidence of harm, in addition to the fact that exercise is known to be beneficial in pregnancy.

Assisted Reproductive Technology Surveillance - United States, 2016.

Problem/condition Since the first U.S. infant conceived with assisted reproductive technology (ART) was born in 1981, both the use of ART and the number of fertility clinics providing ART services have increased steadily in the United States. ART includes fertility treatments in which eggs or embryos are handled in the laboratory (i.e., in vitro fertilization [IVF] and related procedures). Although the majority of infants conceived through ART are singletons, women who undergo ART procedures are more likely than women who conceive naturally to deliver multiple-birth infants. Multiple births pose substantial risks for both mothers and infants, including obstetric complications, preterm delivery ( Period covered 2016. Description of system In 1995, CDC began collecting data on ART procedures performed in fertility clinics in the United States as mandated by the Fertility Clinic Success Rate and Certification Act of 1992 (FCSRCA) (Public Law 102-493 [October 24, 1992]). Data are collected through the National ART Surveillance System (NASS), a web-based data collection system developed by CDC. This report includes data from 52 reporting areas (the 50 states, the District of Columbia, and Puerto Rico). Results In 2016, a total of 197,706 ART procedures (range: 162 in Wyoming to 24,030 in California) with the intent to transfer at least one embryo were performed in 463 U.S. fertility clinics and reported to CDC. These procedures resulted in 65,964 live-birth deliveries (range: 57 in Puerto Rico to 8,638 in California) and 76,892 infants born (range: 74 in Alaska to 9,885 in California). Nationally, the number of ART procedures performed per 1 million women of reproductive age (15-44 years), a proxy measure of the ART use rate, was 3,075. ART use rates exceeded the national rate in 14 reporting areas (Connecticut, Delaware, the District of Columbia, Hawaii, Illinois, Maryland, Massachusetts, New Hampshire, New Jersey, New York, Pennsylvania, Rhode Island, Utah, and Virginia). ART use exceeded 1.5 times the national rate in nine states, including three (Illinois, Massachusetts, and New Jersey) that also had comprehensive mandated health insurance coverage for ART procedures (i.e., coverage for at least four oocyte retrievals). Nationally, among ART transfer procedures for patients using fresh embryos from their own eggs, the average number of embryos transferred increased with increasing age (1.5 among women aged 37 years). Among women aged Interpretation Multiple births from ART contributed to a substantial proportion of all twins, triplets, and higher-order infants born in the United States. For women aged 1.5 times the national average. Although other factors might influence ART use, insurance coverage for infertility treatments accounts for some of the difference in per capita ART use observed among states because most states do not mandate any coverage for ART treatment. Public health action Twins account for almost all of ART-conceived multiple births born in multiple deliveries. Reducing the number of embryos transferred and increasing use of eSET, when clinically appropriate, could help reduce multiple births and related adverse health consequences for both mothers and infants. Because multiple-birth infants are at increased risk for numerous adverse sequelae that cannot be ascertained from the data collected through NASS alone, long-term follow-up of ART infants through integration of existing maternal and infant health surveillance systems and registries with data available from NASS might be useful for monitoring adverse outcomes.

The Support for New Mums Project: A protocol for a pilot randomized controlled trial designed to test a postnatal psychoeducation smartphone application.

To report a pilot trial protocol for testing the effectiveness of the Support for New Mums smartphone application in a cohort of first-time mothers. A pilot/feasibility randomized controlled trial using a two-group pre-test and repeated post-test design. This protocol follows the Standard Protocol Items: Recommendations for Intervention Trials (SPIRIT) guidelines. The Intervention group will receive access to the smartphone application for 6weeks post birth. Both Intervention and control groups will receive standardized institutional postnatal care services. Trial funding was gained from respective grant sponsors in May and November 2016. The Support for New Mums smartphone application could be a novel method for addressing the gap in provision of postpartum care services providing psychoeducation and improving maternal parental self-efficacy for Australian childbearing women. Australian New Zealand Clinical Trials Registry ACTRN12618001580268.

Dynamics of a massive binary at birth

Almost all massive stars have bound stellar companions, existing in binaries or higher-order multiples. While binarity is theorized to be an essential feature of how massive stars form, essentially all information about such properties is derived from observations of already formed stars, whose orbital properties may have evolved since birth. Little is known about binarity during formation stages. Here we report high angular resolution observations of 1.3 mm continuum and H30alpha recombination line emission, which reveal a massive protobinary with apparent separation of 180 au at the center of the massive star-forming region IRAS07299-1651. From the line-of-sight velocity difference of 9.5 km/s of the two protostars, the binary is estimated to have a minimum total mass of 18 solar masses, consistent with several other metrics, and maximum period of 570 years, assuming a circular orbit. The H30alpha line from the primary protostar shows kinematics consistent with rotation along a ring of radius of 12 au. The observations indicate that disk fragmentation at several hundred au may have formed the binary, and much smaller disks are feeding the individual protostars.

Assisted Reproductive Techniques in Latin America: The Latin American Registry, 2015

Research question:What was the utilization, effectiveness and safety of assisted reproductive technologies (ART) performed in Latin American countries during 2015, and what were the regional trends?Design:Retrospective collection of multinational data on assisted reproduction techniques (IVF and intracytoplasmic sperm injection [ICSI], frozen embryo transfer, oocyte donation, preimplantation genetic testing and fertility preservation), from 175 institutions in 15 Latin American countries.Results:In total, 41.25% of IVF/ICSI cycles were performed in women aged 35-39 years, and 28.35% in women aged ≥40 years. After removing freeze-all cycles, delivery rate per oocyte retrieval was 21.39% for ICSI and 24.29% for IVF. Multiple births included 19.58% twins and 0.95% triplets and higher. In oocyte donation, delivery rate per transfer was 36.77%, with a twin and triplet rate of 27.65% and 1.06%, respectively. Overall, preterm deliveries reached 17.38% in singletons, 64.94% in twins and 98.41% in triplets. Perinatal mortality in 14,936 births and 18,391 babies born was 10.5 per 1000 in singletons, 17.9 per 1000 in twins, and 57.1 per 1000 in high-order multiples. Elective single embryo transfer represented 3.11% of fresh transfers, with a 31.78% delivery rate per transfer. Elective double embryo transfer represented 23.3% of transfers, with a 37.79% delivery rate per transfer. Out of 18,391 babies born, 63.22% were singletons, 34.4% twins, and 2.38% triplets and higher.Conclusions:Given the effect of multiple births on prematurity, morbidity and perinatal mortality, reinforcing the existing trend of reducing the number of embryos transferred remains mandatory.

Assisted reproductive techniques in Latin America: the Latin American Registry, 2015.

What was the utilization, effectiveness and safety of assisted reproductive technologies (ART) performed in Latin American countries during 2015, and what were the regional trends? Retrospective collection of multinational data on assisted reproduction techniques (IVF and intracytoplasmic sperm injection [ICSI], frozen embryo transfer, oocyte donation, preimplantation genetic testing and fertility preservation), from 175 institutions in 15 Latin American countries. In total, 41.25% of IVF/ICSI cycles were performed in women aged 35-39 years, and 28.35% in women aged ≥40 years. After removing freeze-all cycles, delivery rate per oocyte retrieval was 21.39% for ICSI and 24.29% for IVF. Multiple births included 19.58% twins and 0.95% triplets and higher. In oocyte donation, delivery rate per transfer was 36.77%, with a twin and triplet rate of 27.65% and 1.06%, respectively. Overall, preterm deliveries reached 17.38% in singletons, 64.94% in twins and 98.41% in triplets. Perinatal mortality in 14,936 births and 18,391 babies born was 10.5 per 1000 in singletons, 17.9 per 1000 in twins, and 57.1 per 1000 in high-order multiples. Elective single embryo transfer represented 3.11% of fresh transfers, with a 31.78% delivery rate per transfer. Elective double embryo transfer represented 23.3% of transfers, with a 37.79% delivery rate per transfer. Out of 18,391 babies born, 63.22% were singletons, 34.4% twins, and 2.38% triplets and higher. Given the effect of multiple births on prematurity, morbidity and perinatal mortality, reinforcing the existing trend of reducing the number of embryos transferred remains mandatory.

Assisted reproductive technology in Latin America: the Latin American Registry, 2017.

What was the utilization, effectiveness and safety of assisted reproductive technologies (ART) carried out in Latin America during 2017. Retrospective collection of multinational data on ART carried out in 188 institutions from 15 Latin American countries. In this study, 93,600 initiated cycles, 16,976 deliveries and 20,404 births reported. Utilization of ART was 221 cycles per million inhabitants (15 to 535). Women aged 40 years and above represented 30.5% of fresh IVF and intracytoplasmic sperm injection (ICSI); however, after removing freeze-all cycles, delivery rate per oocyte retrieval was 19.9% for ICSI and 20.2% for IVF. Overall, single embryo transfer (SET) represented 26.9% of fresh transfers, with 18.2% delivery rate per transfer, increasing to 32.3% in elective SET. Delivery rate in double embryo transfers (DET) was 28.3% increasing to 37.3% with elective DET. This 5% increment in births in elective DET over elective SET resulted in a tenfold increase in twin births, gestational periods almost 3 weeks' shorter and a threefold increase in perinatal mortality. Delivery rate in frozen-thawed SET reached 25.5% increasing to 30.8% with DET, most being blastocyst transfers. Of all births, 66.9% were singletons, 31.4% twins and 1.6% triplets and higher. Overall, preterm deliveries reached 9.5% in singletons, 64.3% in twins and 97.9% in triplets; perinatal mortality was 9.4‰ in singletons, 25.3‰ in twins and 63.3‰ in high-order multiples. The number of initiated cycles has slowly increased. Frozen embryo transfers, blastocyst transfers and SET are also increasing. Our data show that, especially in young women and oocyte recipients, when there is more than one blastocyst for transfer, elective SET should be the rule.

Imprints of white dwarf recoil in the separation distribution of Gaia wide binaries

We construct from Gaia DR2 an extensive and very pure ($\lesssim 0.2\%$ contamination) catalog of wide binaries containing main-sequence (MS) and white dwarf (WD) components within 200 pc of the Sun. The public catalog contains, after removal of clusters and resolved higher-order multiples, $>$50,000 MS/MS, $>$3,000 WD/MS, and nearly 400 WD/WD binaries with projected separations of $50 \lesssim s/{\rm AU} < 50,000$. Accounting for incompleteness and selection effects, we model the separation distribution of each class of binaries as a broken power-law, revealing marked differences between the three populations. The separation distribution of MS/MS systems is nearly consistent with a single power-law of slope $-1.6$ over at least $500 < s/{\rm AU} < 50,000$, with marginal steepening at $s > 10,000$ AU. In contrast, the separation distributions of WD/MS and WD/WD binaries show distinct breaks at $\sim$ 3,000 AU and $\sim$1,500 AU, respectively: they are flatter than the MS/MS distribution at small separations and steeper at large separations. Using binary population synthesis models, we show that these breaks are unlikely to be caused by external factors but can be explained if the WDs incur a kick of $\sim$ 0.75 km s$^{-1}$ during their formation, presumably due to asymmetric mass loss. The data rule out typical kick velocities above 2km s$^{-1}$. Our results imply that most wide binaries with separations exceeding a few thousand AU become unbound during post-MS evolution.

Separation of different order multiples based on focal transform and SRME

Multiples that are reflected more than once beneath the surface are also real reflections from subsurface interfaces. They are typically characterised by smaller reflection angles, longer travel paths and wider illumination ranges than primaries. With the increase in reflected times at the surface, the orders of multiples included in the seismic data will increase. Multiples with higher orders will cover a larger area and contain more structural information. Therefore, if these high order multiples are utilised properly, subsurface structures will be better described. Since the wavefields of different order multiples are extraordinarily complex and research regarding the separation of different order multiples is sparse, conventional techniques for the processing of multiples cannot effectively deal with different order multiples. Considering that the focal transform can flexibly perform order reduction or elevation of multiples and the surface-related multiple elimination (SRME) can effectively separate primaries and multiples, a practical and novel method based on both the focal transform and SRME is proposed to separate different order multiples in this paper. Based on the theoretical derivation, a detailed workflow of the proposed method is provided. Taking full advantages of the focal transform (weighted cross-correlation) and SRME (weighted convolution), the proposed method can effectively separate different order multiples. Numerical examples further illustrate the feasibility and validity of the promising method.

The nature of Earth's correlation wavefield: late coda of large earthquakes

The seismic correlation wavefield constructed from the stacked cross-correlograms of the late coda of earthquake signals at stations across the globe provides a wealth of observed pulses as a function of inter-station distance. The interval from 3 to 10 h after the onset of major earthquakes is employed for the period range from 15 to 50 s. The observations can be well matched by synthetic seismograms for a radially stratified Earth. Many of the correlation phases have similar time behaviour to those in the regular wavefield, but others have no correspondence. All such correlation phases can be explained by the interaction of arrivals with a common slowness at the each of the stations being correlated. Using a generalized ray description of the seismic wavefield, the time-distance behaviour of these correlation phases arises from differences in accumulated phase on different propagation paths through the Earth. Distinct arrivals emerge from the correlation field when there are many ways in which combinations of seismic phases can arise with the same difference in propagation legs. The constituents of the late coda are dominated by steeply travelling waves, and in consequence features associated with multiple passages through the whole Earth emerge distinctly, such as high-order multiples of PKIKP .

A Mixed Lipid Emulsion for Prevention of Parenteral Nutrition Associated Cholestasis in Extremely Low Birth Weight Infants: A Randomized Clinical Trial

ObjectivesTo examine whether a mixed lipid emulsion reduces the incidence of parenteral nutrition associated cholestasis (PNAC) in extremely low birth weight (ELBW, <1000 g) infants.Study designThis double-blind randomized trial of 230 ELBW infants (June 2012-October 2015) was performed at a single level IV neonatal intensive care unit. Patients received either a mixed lipid emulsion composed of soybean oil, medium chain triglycerides, olive oil, and fish oil-(intervention) or a soybean oil-based lipid emulsion (control) for parenteral nutrition. The primary outcome measure was PNAC (conjugated bilirubin >1.5 mg/dL [25 μmol/L] at 2 consecutive measurements). The study was powered to detect a reduction of PNAC from 25% to 10%.ResultsReasons for noneligibility of 274 infants screened were refusal to participate (n = 16), death (n = 10), withdrawal of treatment (n = 5), higher order multiples (n = 9), and parents not available for consent (n = 4). Intention to treat analysis was carried out in 223 infants (7 infants excluded after randomization). Parenteral nutrition associated cholestasis was 11 of 110 (10.1%) in the intervention and 18 of 113 (15.9%) in the control group (P = .20). Multivariable analyses showed no statistically significant difference in the intention to treat (aOR 0.428, 95% CI 0.155-1.187; P = .10) or per protocol population (aOR 0.457, 95% CI 0.155-1.347; P = .16). There was no statistically significant effect on any other neonatal morbidity.ConclusionsThe incidence of parenteral nutrition associated cholestasis was not significantly reduced using a mixed lipid emulsion in ELBW infants.Trial RegistrationClinicalTrials.gov NCT01585935.

Gestational age-specific perinatal mortality rates for assisted reproductive technology (ART) and other births.

Is perinatal mortality rate higher among births born following assisted reproductive technology (ART) compared to non-ART births? Overall perinatal mortality rates in ART births was higher compared to non-ART births, but gestational age-specific perinatal mortality rate of ART births was lower for very preterm and moderate to late preterm births. Births born following ART are reported to have higher risk of adverse perinatal outcomes compared to non-ART births. This population-based retrospective cohort study included 407 368 babies (391 952 non-ART and 15 416 ART)-393 491 singletons and 10 877 twins or high order multiples. All births (≥20 weeks of gestation and/or ≥400 g of birthweight) in five states and territories in Australia during the period 2007-2009 were included in the study, using National Perinatal Data Collection (NPDC). Primary outcome measures were rates of stillbirth, neonatal and perinatal deaths. Adjusted odds ratio (AOR) and 95% confidence interval (CI) were used to estimate the likelihood of perinatal death. Rates of multiple birth and low birthweight were significantly higher in ART group compared to the non-ART group (P < 0.01). Overall perinatal mortality rate was significantly higher for ART births (16.5 per 1000 births, 95% CI 14.5-18.6), compared to non-ART births (11.3 per 1000 births, 95% CI 11.0-11.6) (AOR 1.45, 95% CI 1.26-1.68). However, gestational age-specific perinatal mortality rate of ART births (including both singletons and multiples) was lower for very preterm (<32 weeks' gestation) and moderate to late preterm births (32-36 weeks' gestation) (AOR 0.61, 95% CI 0.53-0.70 and AOR 0.61, 95% CI 0.53-0.70, respectively) compared to non-ART births. Congenital abnormality and spontaneous preterm were the most common causes of neonatal deaths in both ART and non-ART group. Due to different cut-off limit for perinatal period in Australia, the results of this study should be interpreted with cautions for other countries. Australian definition of perinatal period commences at 20 completed weeks (140 days) of gestation and ends 27 completed days after birth which is different from the definition by World Health Organisation (commences at 22 completed weeks (154 days) of gestation and ends seven completed days after birth) and by Centers for Disease Control and Prevention (includes infant deaths under age 7 days and fetal deaths at 28 weeks of gestation or more). Preterm birth is the single most important contributing factor to increased risk of perinatal mortality among ART singletons compared to non-ART singletons. Further research on reducing early preterm delivery, with the aim of reducing the perinatal mortality among ART births is needed. Couples who access ART treatment should be fully informed regarding the risk of preterm birth and subsequent risk of perinatal death. There was no funding associated with this study. No conflict of interest was declared.

Adherence to Safe Sleep Recommendations by Families With Higher-order Multiples.

More than 95% of higher-order multiples are born preterm and more than 90% are low birth weight, making this group of infants especially vulnerable to sudden infant death syndrome (SIDS). Emerging evidence suggests that families with twins face challenges adhering to the American Academy of Pediatrics (AAP) recommendations to reduce SIDS risks. Adherence to the AAP recommendations in families with higher-order multiples has not been described. This study describes SIDS risk reduction infant care practices for higher-order multiples during the first year of life. Mothers caring for higher-order multiple-birth infants were recruited from an online support group. An online survey was used to assess infant care practices when the infants were first brought home from the hospital as well as at the time of the survey. Ten mothers of triplets and 4 mothers of quadruplets responded. Less than 80% of the mothers practiced "back to sleep" immediately postdischarge. Supine sleep positioning decreased over time, particularly during daytime naps. Only 50% of the infants shared the parents' bedroom and approximately 30% bed-shared with their siblings. Sleep-time pacifier use was low. Safe sleep education must include specific questions regarding home sleeping arrangements, encouragement of breast milk feedings, supine positioning, and pacifier use at every sleep for higher-order multiple infants well before discharge in order for parents to plan a safe sleep environment at home. Prospective studies to identify barriers and facilitators can inform future strategies supporting adherence to safe sleep practices for higher-order multiple infants.

P29.05: Placental cord insertion site and adverse pregnancy outcome in twin pregnancy

Abnormal umbilical cord insertion has been associated with unequal sharing of the placental vasculature between twins. A higher prevalence has been reported in pregnancies complicated by selective growth restriction (sFGR), birth weight discordance (BWD). We aimed to investigate the association between cord insertion site and adverse pregnancy outcomes in twin pregnancies. A single-centre cohort study of twin pregnancies that had placental histology. Higher-order multiples, major fetal anomaly, aneuploidy and MCMA pregnancies were excluded. Cord insertion site was categorised into 3 groups: marginal (attachment <2cm to the placental margin), velamentous (attached to the membrane before reaching the placenta with vessels traversing the membranes), normal. Chorionicity, placental weight, number of cord vessels, examination of the membranes, ultrasound findings, maternal characteristics and pregnancy outcome were collected. 546 pregnancies were eligible; 497 (146 monochorionic; 351 dichorionic) were included in the analysis. Significant differences were found between the normal and abnormal cord insertion groups with regards to BWD (P=0.001), BWD>25% (P=0.001), and absolute Z-score differences (P=0.020). Velamentous, but not marginal, cord insertion was significantly associated with sFGR (OR 8.51,95%CI 2.09-34.58;P=0.03) and BWD>25% (OR 11.88,95%CI 3.54-39.79;P=0.04). In MCDA, but not DCDA pregnancies, the rate of composite adverse outcome was higher in those with abnormal cord insertion (70.0% vs 53.0%, p=0.04). Selective fetal growth restriction and birthweight discordance are more common in twin pregnancies with abnormal cord insertion. MCDA twins with velamentous cord insertion are at increased risk of sFGR, and composite adverse pregnancy outcome.

Establishment of the Avera Twin Register in the Midwest USA.

The Avera Twin Register (ATR) aims to study environmental and genetic influences on health and disease using a longitudinal repository of biological specimens, survey data, and health information provided by multiples and their family members. The ATR is located in Sioux Falls, South Dakota, which is a rural and frontier area in the Midwestern United States with a density of four people per square kilometer. The target area of the ATR is South Dakota and the four surrounding states: Minnesota, Iowa, North Dakota, and Nebraska. Enrollment of twins and higher-order multiples of all ages and their family members started on May 18, 2016. A description of the first 13 months of enrollment in this longitudinal register will be provided. The ATR will collect longitudinal data on lifestyle, including diet and activity levels, aging, complex traits, and diseases. Upon registration, all participants are genotyped on the Illumina Global Screening Array (GSA) and twins and higher order multiples receive information on their zygosity. The ATR aims to contribute to large international GWAS consortia and collaborates closely with the Netherlands Twin Register, allowing for the comparison of collected data and analyses of results. In addition, the ATR will address twin-specific questions.

Plurality of Birth and Infant Mortality Due to External Causes in the United States, 2000–2010

(Abstracted from Am J Epidemiol 2017;185(5):335–344) In the United States, external factors, such as accidental injury or assault, account for approximately 5% of infant mortality. While it is known that twins and higher-order multiples are 5 times more likely to die before 1 year of age than singletons, it is not clear how the risk of death due to external causes for multiples compares with that of singletons.

Assisted reproduction techniques in Latin America: the Latin American Registry, 2014

Multinational data on assisted reproduction techniques (IVF and intractytoplasmic sperm injection [ICSI], frozen embryo transfer, oocyte donation, preimplantation genetic diagnosis and fertility preservation) were collected from 159 institutions in 15 Latin American countries. A total of 41.34% of IVF–ICSI cycles were conducted in women aged 35–39 years and 23.35% in women aged 40 years and older. After removing freeze-all cases, delivery rate per oocyte retrieval was 25.05% for ICSI and 27.41% for IVF. Multiple births included 20.78% twins and 0.92% triplets and over. In oocyte donation, twins reached 28.93% and triplets 1.07%. Preterm deliveries reached 16.4% in singletons, 55.02% in twins and 76% in triplets. Perinatal mortality in 18,162 births was 23 per 1000 in singletons, 35 per 1000 in twins, and 36 per 1000 in high-order multiples. Elective single embryo transfer represented 2.63% of fresh transfers, with a 32.15% delivery rate per transfer. Elective double embryo transfer represented 23.74% of transfers, with a 41.03% delivery rate per transfer; 11,373 babies (62.6%) were singletons; 6398 (35.2%) twins, and 391 (2.2%), triplets and more. Given the effect of multiple births on prematurity, morbidity and perinatal mortality, reinforcing the existing trend of reducing the number of embryos transferred is mandatory.

Outcomes of Multifetal Reduction: A Hospital-Based Study.

Higher-order multiple (HOM) pregnancies are associated with increased incidences of pregnancy complications mainly abortions, pre-eclampsia, preterm delivery and fetal death. Multifetal reduction (MFR) during first trimester and subsequent delivery of twins can reduce pregnancy associated morbidities. This study was conducted to evaluate the maternal and fetal outcomes of MFR procedure in patients with HOMs those managed in a tertiary care hospital. It was a prospective observational study carried out in a tertiary care military hospital, India, and all women with higher-order multiples (triplets or more) conceived spontaneously or after infertility treatment (ovulation induction, intra-uterine insemination, or in vitro fertilization) during the 3-year period from Jan 2014 to Dec 2016 were included for MFR. Demographic and clinical data, and obstetric and neonatal outcomes were tabulated. The study included 32 HOM pregnancies which underwent MFR. 16% patients had pre-eclampsia and 12% patients had gestational diabetes. The study had 2 pregnancy losses before 24weeks period of gestation (POG). 70% patients underwent cesarean delivery with mean gestational age of 35.5weeks. Average birth weight of newborn was 1820gm and 80% of them required NICU admission. Favorable pregnancy outcomes can be achieved after multifetal reductions during first trimester in higher-order multiples, but the procedure is not totally safe.

Breastfeeding education and support for women with twins or higher order multiples.

There are rising rates of multiple births worldwide with associated higher rates of complications and more hospital care, often due to prematurity. While there is strong evidence about the risks of not breastfeeding, rates of breastfeeding in women who have given birth to more than one infant are lower than with singleton births. Breastfeeding more than one infant can be more challenging because of difficulties associated with the birth or prematurity. The extra demands on the mother of frequent suckling, coordinating the needs of more than one infant or admission to the neonatal intensive care unit can lead to delayed initiation or early cessation. Additional options such as breast milk expression, the use of donor milk or different methods of supplementary feeding may be considered. Support and education about breastfeeding has been found to improve the duration of any breastfeeding for healthy term infants and their mothers, however evidence is lacking about interventions that are effective to support women with twins or higher order multiples. To assess effectiveness of breastfeeding education and support for women with twins or higher order multiples. We searched the Cochrane Pregnancy and Childbirth Group's Trials Register (30 June 2016), ClinicalTrials.gov (30 June 2016), the WHO International Clinical Trials Registry Platform (ICTRP) (1 July 2016), the excluded studies list from the equivalent Cochrane review of singletons, and reference lists of retrieved studies. Randomised or quasi-randomised trials comparing extra education or support for women with twins or higher order multiples were included. Two review authors independently assessed trials for inclusion and risk of bias, extracted data and checked them for accuracy. We planned to assess the quality of evidence using the GRADE approach, but were unable to analyse any data. We found 10 trials (23 reports) of education and support for breastfeeding that included women with twins or higher order multiples. The quality of evidence was mixed, and the risk of bias was mostly high or unclear. It is difficult to blind women or staff to group allocation for this intervention, so in all studies there was high risk of performance and high or unclear risk of detection bias. Trials recruited 5787 women (this included 512 women interviewed as part of a cluster randomised trial); of these, data were available from two studies for 42 women with twins or higher order multiples. None of the interventions were specifically designed for women with more than one infant, and the outcomes for multiples were not reported separately for each infant. Due to the scarcity of evidence and the format in which data were reported, a narrative description of the data is presented, no analyses are presented in this review, and we were unable to GRADE the evidence.The two trials with data for women with multiple births compared home nurse visits versus usual care (15 women), and telephone peer counselling versus usual care (27 women). The number of women who initiated breastfeeding was reported (all 15 women in one study, 25 out of 27 women in one study). Stopping any breastfeeding before four to six weeks postpartum, stopping exclusive breastfeeding before four to six weeks postpartum, stopping any breastfeeding before six months postpartum andstopping exclusive breastfeeding before six months postpartum were not explicitly reported, and there were insufficient data to draw any meaningful conclusions from survival data. Stopping breast milk expression before four to six weeks postpartum, andstopping breast milk expression before six months postpartum were not reported. Measures ofmaternal satisfaction were reported in one study of 15 women, but there were insufficient data to draw any conclusions; no other secondary outcomes were reported for women with multiple births in either study. No adverse events were reported. We found no evidence from randomised controlled trials about the effectiveness of breastfeeding education and support for women with twins or higher order multiples, or the most effective way to provide education and support . There was no evidence about the best way to deliver the intervention, the timing of care, or the best person to deliver the care. There is a need for well-designed, adequately powered studies of interventions designed for women with twins or higher order multiples to find out what types of education and support are effective in helping these mothers to breastfeed their babies.