High Loss Research Articles

Stem Cells Therapy in Sensorineural Hearing Loss (Systematic Review)

Abstract Background Damage to the inner ear or cochlear nerve results in sensorineural hearing loss (SNHL), which is typically a persistent deafness. SNHL can range in severity from mild to profound. The shape of the audiogram is used to categorise it as high frequency hearing loss, low frequency, flat, peaked, or notched. Pure tone audiometry can be used to diagnose SNHL. Objective To summarization the recent updates in usage of stem cells in sensory neural hearing loss (SNHL). Methods Published studies about using stem cell therapy in ENT practice through comprehensive Pubmed, EKG and Google Scholar search (from 2010-2022). Results Progenitor stem cells may be employed to repair damaged cells and restore sensorineural hearing function, according to 37 of the publications. The majority of these articles—about 90%—discussed animal model-based experimental investigations; the remaining 10% were reviews. Conclusion The application of stem cells in the treatment of SNHL will be a significant step in the future since it will change the way that patients are now treated in the hopes of regaining their hearing. The application to the clinical setting is still in its early stage. Although, a number of encouraging researches illustrate how progenitor stem cells differentiate into sensorineural cells.

Enhanced biomass densification pretreatment using binary chemicals for efficient lignocellulosic valorization

Many effective pretreatment methods (such as dilute acid, dilute alkali, ionic liquids, etc.) have been developed for lignocellulose upgrading, but several defaults of low working mass, high sugar loss and extra cost of solid-liquid separation and water washing hinder their large-scale application in industry. Besides, the valorization of lignin-rich residue from pretreated biomass after hydrolysis or fermentation greatly contributes to the economy and sustainability of lignocellulosic biorefinery, which is usually underestimated. This study developed a densification pretreatment with binary chemicals (densifying lignocellulosic biomass with sulfuric acid (SA) and metal salt (MS) followed by autoclave treatment ((DLCA(SA-MS)), which was conducted under mild condition (121 °C) with a biomass working mass as high as 400 kg/m3. The DLCA(SA-MS) biomass achieved over 95% sugar retention, 90% enzymatic sugar conversion and a high concentration of fermentable sugar (212.3 g/L) with superior fermentability. Furthermore, bio-adsorbent derived from DLCA(SA-MS) biomass residue was highly adsorptive and suitable for dyeing wastewater treatment, providing a feasible and eco-friendly method for lignin-rich residue valorization. These findings indicated that DLCA(SA-MS) pretreatment enables the full-component utilization of biomass and boosts the economic viability of lignocellulosic biorefinery.

Molecular detection and phylogenetic analysis of six bee viruses from Varroa destructor in Korea

In the beekeeping industry, the control of Varroa destructor and viruses is a crucial factor in maintaining the health of the western honey bee Apis mellifera L. Recently, in Korea, beekeepers suffered for high colony losses during the overwintering, and the presence of V. destructor and viruses has been considered an important factor for honey bee health. In this study, we investigated the presence of six honey bee viruses [deformed wing virus (DWV), sacbrood virus (SBV), Kashmir bee virus (KBV), Israeli acute paralysis virus (IAPV), chronic bee paralysis virus (CBPV), and black queen cell virus (BQCV)] in V. destructor in Korea. V. destructor was collected from 46 apiaries in nine provinces in Korea, and viruses were detected using reverse transcription–polymerase chain reaction. DWV was most predominantly detected at 87% of apiaries, followed by IAPV, SBV, KBV, BQCV, and CBPV that were detected in 65.2%, 17.4%, 13%, 8.7%, and 6.5% of apiaries in Korea. Phylogenetic analysis revealed that five viruses (IAPV, SBV, BQCV, and CBPV) were grouped into clades of viruses originating from China, Russia, and Australia, but KBV showed no distinct clades of countries. This study confirmed the presence of various types of bee viruses in V. destructor in Korea, indicating that domestic honey bees are exposed to multiple viruses via V. destructor.

Call to quit: Results of a remote, longitudinal tobacco cessation program.

356 Background: Tobacco cessation counseling remains suboptimal for patients with cancer. The current iteration of the Dana-Farber Cancer Institute Tobacco Cessation Program was established in 2021 to provide virtual, longitudinal tobacco session counseling to patients with cancer. Here, we report the Program’s experience. Methods: The DFCI Tobacco Cessation Program data from 2021 to 2023 was analyzed. Descriptive statistics summarized demographics and tobacco cessation experiences from baseline and follow-up visits. Results: Of 227 patients referred to the Program and included in the analysis (Table 1), 161 declined participation. 63 participants attended a baseline visit, of whom the median age for first cigarette was 15 years (range: 10-32) and the median age for regular tobacco use was 17 years (range: 10-61). Prior to baseline, most participants (n=43) indicated using tobacco daily; the median number of daily cigarettes was 11-15. Many (n=41) reported smoking within 30 minutes of waking up, and most considered tobacco use to be an addiction (n=50). Though a considerable number of participants (n=27) reported quitting smoking to be extremely important to them, confidence in quitting varied; the most frequently selected confidence level was 5/10 (n=14). Past use of tobacco cessation drugs was reported by one-third of participants (n=22); past use of nicotine replacement therapy was reported by nearly half (n=30). The most frequently reported perceived benefit of quitting smoking was better health (n=48). 51 participants had at least one follow-up visit (median: 4, range: 1-23). At the time of first follow-up, 10 reported decreasing the number of cigarettes smoked per day, 20 reported no change, and 3 reported an increase. However, among 24 participants who attended a fifth visit, 8 reported a decrease and 6 reported no change. Of 11 patients who had attended at least 10 visits, 5 reported decreasing their number of daily cigarettes. 13/63 (20.6%) of all participants reported quitting smoking after enrolling, with a median time from baseline assessment to smoke-free status of 34 days. Conclusions: Remote, longitudinal tobacco cessation programs provide an effective way to increase access to tobacco cessation counseling for patients with cancer. However, limitations include high decline rates and loss to follow up. Future analysis of our program will help better understand tobacco cessation efforts. Demographics Approached (n=227) Attended Baseline (n=63) Median Age 60 62 Sex Female 130 (57.3%) | Male 94 (41.4%) |Prefer not to say/Unknown 3 (1.3%) Female 36 (57.1%) |Male 26 (41.3%) |Prefer not to say 1 (1.6%) Cancer Lung 80 (35.2%) |Breast 46 (20.3%) |Head & Neck 27 (11.9%) |GI 24 (10.6%) Lung 22 (34.9%) |Breast 9 (14.3%) |Head & Neck 11 (17.5%) |Lymphoma 6 (9.5%) Race: White 191 (84.1%) 52 (82.5%) Black 17 (7.5%) 4 (6.3%) Asian 4 (1.8%) 1 (1.6%) Other/Prefer not to say 15 (6.6%) 6 (9.5%) Hispanic 8 (3.6%) 3 (5.1%)

Evaluation of Physical and Sensory Properties of Biscuits Made from Blended Wheat Flour and Pumpkin Powder

Biscuit is a type of confectionery popular among all age groups and able to change to become a complete food with necessary nutrients. Pumpkin is a nutritious vegetable that has high post-harvest losses in Sri Lanka. The aim of this research was to analyze the composition of pumpkin powder and evaluate the physical and sensory properties of biscuits made from blended wheat flour and pumpkin powder. Analyses revealed that moisture, protein, fat, ash, crude fiber, and carbohydrate content of pumpkin powder were 8.5%, 5.2%, 1.2%, 3.4%, 4% and 77.7% respectively. Biscuits were made by replacing wheat flour with pumpkin powder at different levels as 0, 5, 10, 20, 30, and 40% (w/w) in the standard formulation. When the percentage of pumpkin powder increased from 10 to 40%, the thickness of biscuits decreased significantly compared to control (0%) There was a significant increment in the spread ratio of biscuits between 10 and 40% levels of pumpkin powder when compared with the control. While 10 to 40% replacement levels of pumpkin powder resulted in significant increase in biscuit hardness, it decreased moisture content significantly compared to control. The color of the biscuits was assessed in terms of lightness, redness, and yellowness. When increasing the pumpkin powder percentage in biscuit, lightness reduced, while redness and yellowness increased gradually. Consumer acceptability test on sensory attributes indicated 5% (w/w) level of pumpkin powder was the most preferred replacement level. The study concludes that pumpkin powder has the potential to be used in biscuit applications.

Turbulent flow field in maglev centrifugal blood pumps of CH-VAD and HeartMate III: secondary flow and its effects on pump performance.

Secondary flow path is one of the crucial aspects during the design of centrifugal blood pumps. Small clearance size increases stress level and blood damage, while large clearance size can improve blood washout and reduce stress level. Nonetheless, large clearance also leads to strong secondary flows, causing further blood damage. Maglev blood pumps rely on magnetic force to achieve rotor suspension and allow more design freedom of clearance size. This study aims to characterize turbulent flow field and secondary flow as well as its effects on the primary flow and pump performance, in two representative commercial maglev blood pumps of CH-VAD and HeartMate III, which feature distinct designs of secondary flow path. The narrow and long secondary flow path of CH-VAD resulted in low secondary flow rates and low disturbance to the primary flow. The flow loss and blood damage potential of the CH-VAD mainly occurred at the secondary flow path, as well as the blade clearances. By contrast, the wide clearances in HeartMate III induced significant disturbance to the primary flow, resulting in large incidence angle, strong secondary flows and high flow loss. At higher flow rates, the incidence angle was even larger, causing larger separation, leading to a significant decrease of efficiency and steeper performance curve compared with CH-VAD. This study shows that maglev bearings do not guarantee good blood compatibility, and more attention should be paid to the influence of secondary flows on pump performance when designing centrifugal blood pumps.

Modeling High Pan Evaporation Losses Using Support Vector Machine, Gaussian Processes, and Regression Tree Models

Modeling High Pan Evaporation Losses Using Support Vector Machine, Gaussian Processes, and Regression Tree Models

The acoustic properties of FDM printed wood/PLA-based composites

The acoustic properties of the Fused Deposition Modelling (FDM) printed PLA wood composite was investigated. Initially tensile and flexural of wood PLA composite was studied with respect to varying layer thickness (0.15 mm, 0.20 mm, and 0.30 mm), infill density (30 %, 60 %, and 90 %), and pattern (Layer, Triangle, and Hexagon). The outcomes demonstrated that the specimen produced with a hexagonal pattern, 90% infill density, and 0.2 mm layer thickness had the highest tensile (16 MPa) and flexural strength (16 MPa). Utilizing this optimized parameter, micro-perforated panels were printed and acoustic properties were studied. Five specimens with a 3 mm thickness, various perforation diameters (5 mm, 4 mm, and 3 mm), and architecturally tapered perforations were fabricated. Using the impedance tube approach, the sound transmission loss and sound absorption coefficients were measured. The findings indicate that, in comparison to all the printed specimens, tapered type perforation with an exterior diameter of 5 mm and an internal diameter of 4.7 mm showed highest sound absorption coefficient of 0.60 Hz. A viscous loss is obtained by its convergent hole diameter reduction, which results in sound attenuations and is easily absorbed in the micro-perforated panel. Similar to this, the specimen printed with smaller perforation diameters (3 mm) had a high sound transmission loss of 79 dB. The small diameter of the perforations prevented the passage of sound waves. The current study is anticipated to lay the groundwork for extensive future research on these classes of materials, potentially serving as a catalyst for advancements in FDM based polymeric materials research and development.

A Novel DC Differential Method for High Frequency Core Loss Measurement

A Novel DC Differential Method for High Frequency Core Loss Measurement

Durum wheat oil oleogels: A study on rheological, thermal, and microstructural properties

Durum wheat oil (WO) is a by-product obtained during wheat milling process characterized by higher amount of bioactive compounds. In recent years, oleogelification, a novel technique to structure oils was developed to replace saturated and trans fats. The aim of this study was to evaluate the performances of WO to obtain stable oleogels using two natural waxes (beeswax BW and carnauba wax CW) at different ratio (4, 7 and 8 %, w/w) respect to sunflower oil (SO) used as control. Oleogels were analyzed for fundamental rheological properties, microstructure and oil loss. Results showed as a concentration below 7 %, weak networks were observed for all samples because the predominance of viscous moduli (G’’) over elastic ones (G’) and high oil loss. Nevertheless at 8 % of waxes, stability in terms of all analyzed parameters was highlighted for all samples (WO_BW showed the lowest). Moreover, WO samples with 7 % and 8 % of CW displayed very high stability in terms of all considered parameters, also at high temperatures, showing strong networks and reaching the optimum solid-like gel without significant differences respect to SO one. Obtained results highlighted the WO ability to be employed, as alternative to SO to develop oleogels with optimal performances and stability.

Biodegradation of glucose and paracetamol and process optimisation with open mixed microbial cultures in lab-scale sequencing batch reactors

In the context of biological wastewater treatment, this study investigated xenobiotics (man-made chemicals) biodegradation and process optimisation to maximise sustainability. Lab-scale sequencing batch reactors (SBRs) fed with paracetamol (model xenobiotic) and glucose (model biogenic substrate) each as only carbon source (feed concentration 1 g L−1) were run in a range of HRTs (hydraulic retention time, range 0.5–2 d) and SRTs (solids retention time, range 1–28 d). For both substrates, the removal of COD (chemical oxygen demand) increased as the SRT increased, reaching 97 % for glucose and 86 % for paracetamol. At the lowest HRT, the high solid losses with the effluent caused a reduction in the SRT with worse substrate removal. The fraction of the removed COD which was converted into biomass increased for lower SRT and overall the best performance for sustainability (compromise between substrate removal, energy consumption and plant footprint) was observed with HRT 1 d and SRT 11–12 d. The experimental data were modelled using two different approaches: linearisation of a continuous-flow model and non-linear parameter fitting with a periodic steady-state SBR model. The results indicated faster growth rate and slightly higher growth yield for glucose than for paracetamol.

Modelling of Control Strategy for Sun Tracking of Concentrated Photovoltaic (CPV) Systems and Performance Analysis of Optical Losses Due to Tracking Errors.

Abstract Among the wide range of solar energy technologies, CPV remains a promising technology worldwide. The photovoltaic concentrator maximizes its efficiency through the use of optical concentration systems. But since optical concentration depends on direct solar radiation, maximizing the quantity of useful solar radiation received requires using a solar tracker that tracks the direction of the sun. To maximize collector efficiency, it’s crucial to choose tracking systems that align with the optical concentration factor. Inadequate focusing of direct solar radiation on the concentrator’s receiver can result in high optical losses, regardless of quality. This paper focuses on improving the accuracy and lowering the cost of the tracker trajectory generation strategy. A sun tracker simulator is first developed, allowing for the investigation of the impact of sun tracking performance a traditional control strategy is initially implemented that uses an astronomical calculation to follow the path of the sun. This strategy uses a sun position algorithm from the National Renewable Energy Laboratory to generate the tracker trajectory and track the sun’s path. When tested on the simulator, as a result, this strategy demonstrated a level of accuracy that ensured a good balance between intricacy and accuracy. Then, An investigation study was conducted to determine how this solar tracker’s precision might affect its optical efficiency. the optical losses resulting from the tracking were calculated using the provided location, The optical loss calculated for this collector’s maximum tracking error is approximately 9%. The research methodology showed that the tracker precision satisfied the requirements of the optical system under study.

Field trial of stable radio frequency transfer system in 200 km metropolitan optical fiber link.

In this paper, we demonstrate stable radio frequency (RF) transfer via metropolitan optical fiber link in the Beijing area. The phase variation of the RF signal is compensated by a phase conjugation method incorporating two high-performance phase-locked loops. The wavelength conversion module extends the transmission length to 200 km with only two parallel 50 km dark optical fibers available. We optimize the configuration of dispersion compensation and optical amplification due to the high loss (0.31 dB/km) of the optical fiber link. At the same time, comparative experiments verify the short-term instability limitation that arises from the group velocity dispersion of the optical fiber link. The measured standard Allan deviation of the 2.4GHz RF transmission system with dispersion compensation is 4.5 × 10-14/1 and 2.6 × 10-17/20 000s, which is superior to that of the reference rubidium clock. The short-term instability of the system is deteriorated to 2.5 × 10-13/1s without dispersion compensation.

Risk Management Analysis Based on ISO 31000:2018 at Andi Djemma Masamba Airport, Luwu Regency

The density of activities that take place around the operating facilities at Andi Djemma Masamba Airport so that the Management of the Andi Jemma Airport Operator Unit (UPBU) Office has launched the development of Andi Djemma Masamba Airport as an aviation company so that it continues to carry out various facility improvements, especially in risk prevention with potential activities with high losses, therefore it is necessary to conduct research to find out how to map, risk assessment and design a risk control and mitigation system at the Operations facility at Andi Djemma Masamba Airport caused by activities that have the potential to cause risk. The stages of the risk management process refer to ISO 31000: 2018 including risk identification, risk analysis and risk evaluation. Expected impact and likelihood of occurrence are mapped through the risk matrix from the Federation Aviation Administration (FAA). The results showed that at the andi djemma masamba airport there were 6 events that fell into the red category, namely Aircraft accidents during take off/landing, Aircraft accidents in the apron area, Escape of dangerous goods into the aircraft, Fuel Spills on the Apron/Taxiway, Lack of building maintenance, The security inspection process is less than optimal.

Quantum enhancement detection techniques for FMCW LiDAR.

Interferometric LiDAR is a device that is used to achieve distance, velocity and phase estimation with high precision and resolution through the use of frequency-modulated continuous wave (FMCW). In this instance, we study quantum enhancement detection techniques for a Mach-Zender interferometer with a FMCW coherent state input. Various quantum detection methods-including NOON state detection, coincidence detection, and sum of parity detection-are applied to the FMCW coherent state and compared against the classical heterodyne detection technique. The findings reveal the potential to trade maximum detectable distance for resolution enhancement. Furthermore, classical Fisher information is utilized to validate and quantify the precision limits of each detection technique. In scenarios characterized by high losses, it is observed that the precision limits of coincidence detection, sum of parity detection, and classical detection techniques are comparable. Therefore, this study offers practical guidance for designing quantum-enhanced receivers for FMCW LiDAR systems.

Systemic Metabolic and Volumetric Assessment via Whole-Body [18F]FDG-PET/CT: Pancreas Size Predicts Cachexia in Head and Neck Squamous Cell Carcinoma.

Background/Objectives: Cancer-associated cachexia in head and neck squamous cell carcinoma (HNSCC) is challenging to diagnose due to its complex pathophysiology. This study aimed to identify metabolic biomarkers linked to cachexia and survival in HNSCC patients using [18F]FDG-PET/CT imaging and machine learning (ML) techniques. Methods: We retrospectively analyzed 253 HNSCC patients from Vienna General Hospital and the MD Anderson Cancer Center. Automated organ segmentation was employed to quantify metabolic and volumetric data from [18F]FDG-PET/CT scans across 29 tissues and organs. Patients were categorized into low weight loss (LoWL; grades 0-2) and high weight loss (HiWL; grades 3-4) groups, according to the weight loss grading system (WLGS). Machine learning models, combined with Cox regression, were used to identify survival predictors. Shapley additive explanation (SHAP) analysis was conducted to determine the significance of individual features. Results: The HiWL group exhibited increased glucose metabolism in skeletal muscle and adipose tissue (p = 0.01), while the LoWL group showed higher lung metabolism. The one-year survival rate was 84.1% in the LoWL group compared to 69.2% in the HiWL group (p < 0.01). Pancreatic volume emerged as a key biomarker associated with cachexia, with the ML model achieving an AUC of 0.79 (95% CI: 0.77-0.80) and an accuracy of 0.82 (95% CI: 0.81-0.83). Multivariate Cox regression confirmed pancreatic volume as an independent prognostic factor (HR: 0.66, 95% CI: 0.46-0.95; p < 0.05). Conclusions: The integration of metabolic and volumetric data provided a strong predictive model, highlighting pancreatic volume as a key imaging biomarker in the metabolic assessment of cachexia in HNSCC. This finding enhances our understanding and may improve prognostic evaluations and therapeutic strategies.

Enhancing the Efficiency of Flexible, Large-Area, ITO-Free Organic Photovoltaic Devices.

The development of flexible ITO-free devices is crucial for the industrial advancement of organic photovoltaic (OPV) technology. Here, a novel ITO-free device architecture is proposed, and ITO-free OPV devices are realized on glass substrates with performance comparable to that of ITO-based devices. It is also demonstrated that the performance of ITO-free devices on polyethylene terephthalate (PET) substrates is limited due to the higher surface roughness of PET, leading to high voltage losses, low device quantum efficiency, and high device leakage current. To address the issue of high roughness on the PET surface, a polyimide (PI) modification strategy is developed and the PI-modified PET is employed as the substrate to construct flexible ITO-free OPV devices and large-area modules with an active area of up to 16.5cm2. This approach leads to decreased trap-assisted recombination losses, enhanced exciton dissociation efficiency, and a reduced density of pinholes in flexible OPV devices, resulting in improved photovoltaic performance under both strong and weak illumination conditions. The outcomes of this work are expected to advance the industrial development of flexible organic photovoltaic technology.

Low sintering temperature, interface characteristic and microwave/terahertz dielectric properties of ternary-phase Nd2O3-P2O5 based ceramics for patch antenna application

In this work, LiF was chosen as a sintering aid to lower the sintering temperature required for monoclinic NdPO4 (NPO) ceramic. A comprehensive investigation was conducted to analyze the impact of LiF on the sintering characteristics, microstructures, phase compositions, crystalline structures, interface characteristics, and microwave dielectric properties of NPO ceramic. Structure-property relationships were discussed based on the density, crystallite size, and lattice strain. The complex impedance spectroscopy revealed the mechanism underlying the interface characteristics of LiF on NPO ceramic. Additionally, far-infrared reflectance spectra and THz time-domain spectra revealed the intrinsic dielectric properties of NdPO4–1 wt.%LiF (NPO1). Notably, 1 wt.% LiF effectively reduced the sintering temperature from 1300 °C to 850 °C, significantly improving densification and dielectric properties. Typical properties such as Q × f = 55,840 GHz (at 9.58 GHz), εr = 10.4, and τf = −43.1 ppm °C−1 were obtained for NPO1 ceramic after sintering at 850 °C. Furthermore, its compatibility with Ag was explored as a prerequisite for LTCC applications. Finally, the NPO1 ceramic was designed as a microstrip antenna with high gain (6.83 dB) and low return loss (−18.85 dB) at a centre frequency of 2.56 GHz, demonstrating its potential for Wi-Fi and Bluetooth applications. This work provides a theoretical foundation and practical guidance for developing orthophosphate ceramics.

Optimizing Magnetic Separation and Cleaning Module in Fully Automated Chemiluminescence Immunoassay Analyzer Using a Special Arrangement of Spliced Magnets and a Three-Stage Magnetic Bead Collection Method

The magnetic separation and cleaning module, as a core component of the fully automated chemiluminescence immunoassay (CLIA) analyzer, encounters issues including high magnetic bead loss rate, long cleaning time, and poor cleaning effect. Based on a simulation analysis using COMSOL, we proposed a novel magnetic separation and cleaning module applied to a fully automated CLIA analyzer. The module adopted a method of arranging spliced rectangular magnets on opposite sides, where the same polarity faced each other, as well as a three-stage magnetic bead collection method. With the proposed method, the total cleaning process can be accomplished within 225 s; the total magnetic bead loss rate over three rounds of cleaning is 6.03%, whereas that of traditional instruments is 25.85%; the coefficient of variation (CV) of the magnetic bead loss rate is less than 0.5%; effective cleaning of free markers is achieved under various sample conditions. Compared with traditional CLIA instruments, this method comprehensively improves key performance indicators of the magnetic separation and cleaning module, providing a reference for similar modules in fully automated CLIA analyzers and positively impacting the accuracy of CLIA for the detection of disease biomarkers.

In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium Columnare

Flavobacterium columnare is a virulent intracellular bacterial pathogen that causes an infection known as columnaris in many species of fish. Some economically important fish species are strongly affected by columnaris, leading to a high mortality rate and significant economic losses. Previous in silico studies have provided various biological insights into F. columnare, including its interaction with MHC class I alleles and the epitopic region within outer membrane proteins. However, the protein-protein interaction networks underlying the growth, defense, and pathogenesis of F. columnare remain largely unknown. This study was conducted to identify the protein-protein interaction (PPI) networks and hub proteins of F. columnare that can be used as drug or vaccine targets. A total of 500 protein sequences were retrieved from UniprotKB in FASTA format and analyzed using VaxiJen, PSORTb, STRING, Cytoscape, and BLASTp programs. The results demonstrated that 60% of F. columnare proteins were predicted as antigenic proteins, most of which were associated with catalytic activity and metabolic processes, identified as cytoplasmic proteins. Ten hub proteins with the highest number of functional interactions were identified, which were also antigenic and non-host homologous. In conclusion, F. columnare hub proteins represent potential therapeutic targets in drug and vaccine development against columnaris infection.