High Salt Levels Research Articles

Role of Rhizosphere Bacteria Isolated from Wild-type Varieties of Sundarbans to Endure Salt Tolerance

Bangladesh is a low-lying riverine country where a large number of populations live in the saline zone. Salinization is a burning issue around the world that affect the plant growth as well as crop production. Due to soil salinization, a fifth of the world's agricultural land has been spoiled. From the geographical setting, Bangladesh is one of the most vulnerable countries to the impact of various natural hazards and disasters. Sundarbans also experienced such natural hazards and disasters, e.g., tsunamis, floods, and cyclones every year for being its location. Moreover, the study area's salinization level is increasing daily. As a result, the plants in the study area are facing a critical situation due to the rapidly changing saline levels. The study attempted to analyze rhizosphere bacteria isolated from wild-type varieties of Sundarbans to endure salt tolerance. This study was conducted using primary and secondary data. Wild-type variety and soil samples were collected from the Sundarbans. To isolate salt-tolerant bacteria, different concentrations of salt were used. The salt tolerance isolates were used to observe the role of seed germination and plant growth. The isolated salt-tolerant bacteria showed their effects on plant growth and seed germination in saline soil. Bacillus and Acinetobacter bacterial species were identified using 16S rRNA gene sequencing, which promoted plant growth and seed germination at a high salt level. Since rhizosphere bacteria play an essential role in enduring salt tolerance, it can be a straightforward and eco-friendly strategy to reduce plant salt stress. J. of Sci. and Tech. Res. 4(1): 71-80, 2022

PRODUCTIVITY ASSESSMENT OF VARIOUS PLANT COMMUNITIES AT URANIUM MINE SITES IN CENTRAL KAZAKHSTAN

Examining the overgrowth of dumps, techno-soils, and areas containing waste products of uranium mines is vital for understanding the dynamic features of vegetation cover in technogenic landscapes. The main aim of this study was to investigate variations in the plant species composition and their productivity in dumps and technological areas and the intensity of soil ionizing radiations under varying environmental conditions based on the Shantobe Uranium Deposit, Kazakhstan. The vegetation at the waste dumps and technogenic sites is in the early stages of syngenesis and is representative of pioneer and group-thicket communities. Adverse ecological conditions associated with intense sulfate salinization formation thrive at the technological sites. However, the floristic composition is illustrative of highly resistant species (Calamagrostis epigejos and Phragmites australis) and secondary species. Typically the formation of steppe zone plant communities of Kazakhstan does not occur in these sites. The productivity of the recultivated dump and banks of the former uranium mine is quite high at 120–150 g/m2, which matches the meadow-ruderal communities of Northern Kazakhstan. However, the lowest productivity of 30–37 g/m2 emerged in the non-recultivated and partially processed sulfuric acid heap leaching stacks containing uranium ore, which create exceedingly unfavorable conditions for the establishment of crop plants. Several plant species identified as self-seeding live in partially processed piles of sulfuric acid heap-leaching uranium ores with a sufficient level of resistance to survive in soil with high levels of sulfate-containing salts and ionizing radiation of 1200–1400 μR/hr. These facts can authenticate to consider the possibility of growing these plant species in the artificial grassing of uranium-containing dumps to create herbage.

Ultraprocessed plant-based foods: Designing the next generation of healthy and sustainable alternatives to animal-based foods.

Numerous examples of next-generation plant-based foods, such as meat, seafood, egg, and dairy analogs, are commercially available. These products are usually designed to have physicochemical properties, sensory attributes, and functional behaviors that match those of the animal-sourced products they are designed to replace. However, there has been concern about the potential negative impacts of these foods on human nutrition and health. In particular, many of these products have been criticized for being ultraprocessed foods that contain numerous ingredients and are manufactured using harsh processing operations. In this article, the concept of ultraprocessed foods is introduced and its relevance to describe the properties of next-generation plant-based foods is discussed. Most commercial plant-based meat, seafood, egg, and dairy analogs currently available do fall into this category, and so can be classified as ultraprocessed plant-based (UPB) foods. The nutrient content, digestibility, bioavailability, and gut microbiome effects of UPB foods are compared to those of animal-based foods, and the potential consequences of any differences on human health are discussed. Some commercial UPB foods would not be considered healthy based on their nutrient profiles, especially those plant-based cheeses that contain low levels of protein and high levels of fat, starch, and salt. However, it is argued that UPB foods can be designed to have good nutritional profiles and beneficial health effects. Finally, areas where further research are still needed to create a more healthy and sustainable food supply are discussed.

Increased oxidative stress responses in murine macrophages exposed at the air-liquid interface to third- and fourth-generation electronic nicotine delivery system (ENDS) aerosols

BackgroundNew fourth generation electronic nicotine delivery system (ENDS) devices contain high levels of nicotine salt (up to 60 mg/mL), whose cellular and molecular effects on immune cells are currently unknown. Here, we used a physiologically-relevant in vitro air-liquid interface (ALI) exposure model to assess the toxicity of distinct ENDS, a 3rd-generation electronic-cigarette (e-cig) and two 4th-generation ENDS devices (JUUL and Posh Plus). MethodsMurine macrophages (RAW 264.7) were exposed at the ALI to either air, Menthol or Crème Brûlée-flavored ENDS aerosols generated from those devices for 1-hour per day for 1 or 3 consecutive days. Cellular and molecular toxicity was evaluated 24 h post-exposure. Results1-day of Menthol-flavored JUUL aerosol exposure significantly decreased cell viability and significantly increased lactate dehydrogenase (LDH) levels compared to air controls. Further, JUUL Menthol elicited significantly increased reactive oxygen species (ROS) and nitric oxide (NO) production compared to air controls. Posh Crème Brûlée-flavored aerosols displayed significant cytotoxicity – decreased cell viability and increased LDH levels –after 1- and 3-day exposures, while the Crème Brûlée-flavored aerosol produced by the 3rd-generation e-cig device only displayed significant cytotoxicity after 3 days compared to air controls. Further, both Posh and third-generation e-cig Crème Brûlée flavored-aerosols elicited significantly increased ROS plus high levels of 8-isoprostane after 1 and 3 days compared to air controls, indicating increased oxidative stress. Posh and third-generation e-cig Crème Brûlée flavored-aerosols elicited reduction in NO levels after one day, but elicited increase in NO after 3 days. Genes in common dysregulated by both devices after 1 day included α7nAChR, Cyp1a1, Ahr, Mmp12, and iNos. ConclusionOur results suggest that ENDS Menthol and Crème Brûlée-flavored aerosol exposures from both 3rd- and 4th-generation ENDS devices are cytotoxic to macrophages and cause oxidative stress. This can translate into macrophage dysfunction. Although 4th-generation disposable ENDS devices have no adjustable operational settings and are considered low-powered ENDS devices, their aerosols can induce cellular toxicity compared to air-exposed control cells. This study provides scientific evidence for regulation of nicotine salt-based disposable ENDS products.

Ecophysiology of Mentha piperita under saline stress and biostimulant in the Brazilian semiarid region

Brazilian semiarid region has water with high levels of salts that limit its use in agriculture, necessitating the adoption of strategies to reduce the impact of salinity. The objective of this study was to assess the effect of a biostimulant as a attenuating of mitigate saline stress on the growth, gas exchange, chlorophyll indices, and chlorophyll a fluorescence of Mentha piperita. The experiment was performed using a randomized block design, employing a 5 × 5 incomplete factorial schemes. This involved five different electrical conductivities of the irrigation water (ECw = 0.5, 1.01, 2.25, 3.49, and 4.00 dS m-1) and five doses of the biostimulant (Stimulate® = 0.0, 1.45, 5.00, 8.55, and 10.0 mL L-1). In total, nine combinations were generated using the Central Composite Design. Growth, gas exchange, chlorophyll indices, and chlorophyll a fluorescence were evaluated 45 days after irrigation with saline water. The results indicated that saline stress hindered the growth, gas exchange, chlorophyll indices, and photochemical efficiency of M. piperita. However, the biostimulant mitigated the adverse effects of salinity on the growth, gas exchange, and photochemical efficiency of M. piperita.

Genomic Dynamics and Functional Insights under Salt Stress in Gossypium hirsutum L.

The changing climate is intensifying salt stress globally. Salt stress is a menace to cotton crop quality and yield. The seedling, germination, and emergence phases are more prone to the effects of salt stress than other stages. Higher levels of salt can lead to delayed flowering, a reduced number of fruiting positions, shedding of fruits, decreased boll weight, and yellowing of fiber, all of which have an adverse effect on the yield and quality of the seed cotton. However, sensitivity toward salt stress is dependent on the salt type, cotton growth phase, and genotype. As the threat of salt stress continues to grow, it is crucial to gain a comprehensive understanding of the mechanisms underlying salt tolerance in plants and to identify potential avenues for enhancing the salt tolerance of cotton. The emergence of marker-assisted selection, in conjunction with next-generation sequencing technologies, has streamlined cotton breeding efforts. This review begins by providing an overview of the causes of salt stress in cotton, as well as the underlying theory of salt tolerance. Subsequently, it summarizes the breeding methods that utilize marker-assisted selection, genomic selection, and techniques for identifying elite salt-tolerant markers in wild species or mutated materials. Finally, novel cotton breeding possibilities based on the approaches stated above are presented and debated.

The Disastrous Effects of Soil Salinity and pH on Environmental Systems

Soil salinity is a natural element of arid and semi-arid climates and it is becoming a growing concern in the soils across the world. When water-soluble salts build in a soil, it gets salinized. These salts contain chloride, sulphate, carbonate, bicarbonate and sodium in addition to potassium and magnesium. Due to shortage of oxygen, soil with a high salt level becomes incapable of supporting plant and animal life. This review discusses the causes of salinity, its impact on plant growth, their limits/standard in the environment systems and case studies of saline land. Besides this, salinity levels in streams and lands are generally rising as a result of rising groundwater levels. Most of the rural and urban communities have lost productive cropland and water supplies due to natural instability to these environments and human induced interferences. Crop productivity, seed germination soil and water quality are adversely affected by soil salinity. A coastal region is also particularly vulnerable to climate change. There is a need to study soil salinization and its measures in detail for sustainable environmental systems.

The Assessment of the Groundwater Quality in the Coastal Aquifers of the Essaouira Basin, Southwestern Morocco, Using Hydrogeochemistry and Isotopic Signatures

Because of anthropogenic activity and seawater intrusion, coastal aquifers worldwide frequently face a threat to their water supply due to salinization. This paper investigates the assessment of the groundwater quality in coastal aquifers of the Hauturivien aquifer in the Essaouira basin. In this study, 56 groundwater samples collected from the Hauturivian aquifer across four campaigns in 2017, 2018, 2019, and 2020 were subjected to multivariate analyses involving principal component analysis (PCA) and cluster analysis (CA) using SPSS software. Among the three main water types, the mixed Ca-Mg-Cl classification was predominant in the investigated aquifer. In addition to the natural processes (such as the water–rock interaction, ion exchange, dissolution/precipitation dynamics, and evaporation) that govern groundwater quality, current land use practices have increased salinization in this poorly drained semi-arid area. Based on assessments using Water Quality Index (WQI) and Irrigation Water Quality Index (IWQI), the water quality is suitable for human consumption, but its use for irrigation is limited to crops that can tolerate high salt levels. The stable isotopes (δ2H and δ18O) of groundwater demonstrated that local precipitation is the primary recharge source. Nonetheless, the evaporation process, influenced by various geological conditions, affects groundwater recharge, regardless of the topographical differences in the study area.

Designing healthier plant-based foods: Fortification, digestion, and bioavailability

Many consumers are incorporating more plant-based foods into their diets as a result of concerns about the environmental, ethical, and health impacts of animal sourced foods like meat, seafood, egg, and dairy products. Foods derived from animals negatively impact the environment by increasing greenhouse gas emissions, land use, water use, pollution, deforestation, and biodiversity loss. The livestock industry confines and slaughters billions of livestock animals each year. There are concerns about the negative impacts of some animal sourced foods, such as red meat and processed meat, on human health. The livestock industry is a major user of antibiotics, which is leading to a rise in the resistance of several pathogenic microorganisms to antibiotics. It is often assumed that a plant-based diet is healthier than one containing more animal sourced foods, but this is not necessarily the case. Eating more fresh fruits, vegetables, nuts, and whole grain cereals has been linked to improved health outcomes but it is unclear whether next-generation plant-based foods, such as meat, seafood, egg, and dairy analogs are healthier than the products they are designed to replace. Many of these new products are highly processed foods that contain high levels of saturated fat, sugar, starch, and salt, and low levels of micronutrients, nutraceuticals, and dietary fibers. Moreover, they are often rapidly digested in the gastrointestinal tract because processing disrupts plant tissues and releases the macronutrients. Consequently, it is important to formulate plant-based foods to reduce the levels of nutrients linked to adverse health effects and increase the levels linked to beneficial health effects. Moreover, it is important to design the food matrix so that the macronutrients are not digested and absorbed too quickly, but the micronutrients are highly bioavailable. In this article, we discuss how next-generation plant-based foods can be made healthier by controlling their nutrient profile, digestibility, and bioavailability.

Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis.

Intrinsically disordered proteins play important roles in cell signaling, and dysregulation of these proteins is associated with several diseases. Prostate apoptosis response-4 (Par-4), an approximately 40 kilodalton proapoptotic tumor suppressor, is a predominantly intrinsically disordered protein whose downregulation has been observed in various cancers. The caspase-cleaved fragment of Par-4 (cl-Par-4) is active and plays a role in tumor suppression by inhibiting cell survival pathways. Here, we employed site-directed mutagenesis to create a cl-Par-4 point mutant (D313K). The expressed and purified D313K protein was characterized using biophysical techniques, and the results were compared to that of the wild-type (WT). We have previously demonstrated that WT cl-Par-4 attains a stable, compact, and helical conformation in the presence of a high level of salt at physiological pH. Here, we show that the D313K protein attains a similar conformation as the WT in the presence of salt, but at an approximately two times lower salt concentration. This establishes that the substitution of a basic residue for an acidic residue at position 313 alleviates inter-helical charge repulsion between dimer partners and helps to stabilize the structural conformation.

TOLERANCE OF ALFALFA VARIETIES SUBJECTED TO SALINITY

The aim of this study was to evaluate the salinity tolerance of alfalfa varieties, aiming at their inclusion in production systems that use water with high levels of salts for irrigation or saline soils. The experiment was conducted in a greenhouse, in a 3x5x3 factorial scheme, three alfalfa varieties (LE-N4, Crioula and DK-192), five salinity levels (0; 3.5; 5.0; 7.0; 10 .5 and 14.0 dS m-1) and three shoots of the aerial part, with four repetitions. For irrigation management, lysimeters were installed. Fresh and dry aboveground and root biomass, chlorophyll, water use efficiency and enzymatic activity were analyzed. The increase in salinity reduced fresh and dry aboveground and root biomass, chlorophyll and water use efficiency. Salinity altered the biochemical responses of the evaluated varieties. DK-192 showed higher production of fresh and dry aboveground biomass. LE-N4 was more sensitive to salt stress, significantly reducing production from a salinity level of 7.0 dS m-1. The increased catalase activity for Crioula and DK-192 under salt stress conditions contributed to the higher production of fresh and dry aboveground biomass.

Effects of salt stress on interspecific competition between an invasive alien plant Oenothera biennis and three native species.

Biological invasions and soil salinization have become increasingly severe environmental problems under global change due to sea-level rise and poor soil management. Invasive species can often outcompete native species, but few studies focus on whether invasive alien species are always superior competitors under increasing stressors. We grew an invasive grass species, Oenothera biennis L., and three native grass species (Artemisia argyi Lévl. et Vant., Chenopodium album L., and Inula japonica Thunb.) as a monoculture (two seedlings of each species) or mixture (one seedling of O. biennis and one native species seedling) under three levels of salt treatments (0, 1, and 2 g/kg NaCl) in a greenhouse. We found that invasive O. biennis exhibited greater performance over native C. album and I. japonica, but lower performance compared to A. argyi, regardless of the soil salinity. However, salinity did not significantly affect the relative dominance of O. biennis. Interspecific competition enhanced the growth of O. biennis and inhibited the growth of I. japonica. Although O. biennis seedlings always had growth dominance over C. album seedlings, C. album was not affected by O. biennis at any salt level. At high salt levels, O. biennis inhibited the growth of A. argyi, while A. argyi did not affect the growth of O. biennis. Salt alleviated the competitive effect of O. biennis on I. japonica but did not mitigate the competition between O. biennis and the other two native species. Therefore, our study provides evidence for a better understanding of the invasive mechanisms of alien species under various salinity conditions.

Revitalisasi Usaha Kesehatan Sekolah (UKS) Melalui Edukasi Sekolah Sehat di Sekolah Dasar Negeri 01 Kebalen Bekasi

The pandemic that has been going on for more than two years has changed many things, including the activity patterns of students. Not only that, this condition is exacerbated by the poor eating habits of students in various schools. problems related to the nutritional condition of students. The nutritional condition of SD Negeri Kebalen 01 Bekasi students is closely related to the consumption patterns adopted by students in their daily life in the school environment. Students seem to enjoy consuming snacks and drinks with high levels of sugar, flavourings, and salt. The next problem that is seen in students is the drastic reduction in physical activity carried out by students outside the school environment. This is due to the habits of students who spend more time playing smartphones and the various game applications contained in them. The purpose of carrying out this community service activity is to instill an understanding of the importance of healthy living behavior through strengthening the 'Healthy Schools' campaign. The method used in this activity is counseling. The results of the activity show that the activity has been running without significant obstacles where the participants' involvement in the whole extension process shows high enthusiasm and interest. The existence of interest, attention and high motivation makes the potential of participants in understanding the material presented wide open implementation.

What's in a commercial meal kit? Structured review of Australian meal kits.

The aim of this project is to review the products and recipes contained within popular subscription meal kits to determine if they are suitable for wider use among people who are food insecure. Across the 6-week period, weekly meal kits from both HelloFresh and Markey Spoon were purchased, resulting in thirty-six individual meals that were prepared and assessed. Meals were assessed based on the content included in the meal kit compared with the recipe card and the nutrition panel, the costs of the individual foods if purchased at one of two major supermarkets and the ease of preparation. Australia. Households were comprised of two, 2-person households who were provided with 2 meals each week, and two, single-person households who were provided with one meal each week. The findings of this research suggest that while the meal kits are convenient and, in general, the recipes are easy to follow, and the meals would be made again, the high levels of salt and fat may preclude these kits from regular inclusion in a healthy diet. The meal kits were also found to be more costly than the same ingredients if purchased from a major supermarket. However, the convenience of having most of the foods needed to prepare a full meal with little to no wastage may counterbalance this cost. Meal kits may be a useful component of a healthy diet, that can increase meals prepared and consumed in the home, and thanks to the clear instructions and pre-portioned ingredients, may reduce stress related to food preparation.

Salinity and high pH reduce denitrification rates by inhibiting denitrifying gene abundance in a saline-alkali soil

Denitrification, as the main nitrogen (N) removal process in farmland drainage ditches in coastal areas, is significantly affected by saline-alkali conditions. To elucidate the effects of saline-alkali conditions on denitrification, incubation experiments with five salt and salt-alkali gradients and three nitrogen addition levels were conducted in a saline-alkali soil followed by determination of denitrification rates and the associated functional genes (i.e., nirK/nirS and nosZ Clade I) via N2/Ar technique in combination with qPCR. The results showed that denitrification rates were significantly decreased by 23.83–50.08%, 20.64–57.31% and 6.12–54.61% with salt gradient increasing from 1 to 3‰, 8‰, and 15‰ under 0.05‰, 0.10‰ and 0.15‰ urea addition conditions, respectively. Similarly, denitrification rates were significantly decreased by 44.57–63.24% with an increase of the salt-alkali gradient from 0.5 to 8‰. The abundance of nosZ decreased sharply in the saline condition, while a high salt level significantly decreased the abundance of nirK and nirS. In addition, the increase of nitrogen concentration attenuated the reduction of nirK, nirS and nosZ gene abundance. Partial least squares regression (PLSR) models demonstrated that salinity, dissolved oxygen (DO) in the overlying water, N concentration, and denitrifying gene abundance were key determinants of the denitrification rate in the saline environment, while pH was an additional determinant in the saline-alkali environment. Taken together, our results suggest that salinity and high pH levels decreased the denitrification rates by significantly inhibiting the abundance of the denitrifying genes nirK, nirS, and nosZ, whereas increasing nitrogen concentration could alleviate this effect. Our study provides helpful information on better understanding of reactive N removal and fertilizer application in the coastal areas.

Population density and distribution patterns of Austruca annulipes (Ring-legged fiddler crab) in the mangrove of Bogowonto Estuary, Kulon Progo, Yogyakarta, Indonesia

Abstract. Fadilah RN, Yulia IT, Alfitra ZS, Armadhan WS, Widyaningtyas R, Rahmayani D, Permatasari DP, Igustita, Kusuma D, Prambudi SA, Berlin GE, Triyanto A, Sutarno, Indrawan M, Dadiono MS, Rahim KABA, Setyawan AD. 2023. Population density and distribution patterns of Austruca annulipes (Ring-legged fiddler crab) in the mangrove of Bogowonto Estuary, Kulon Progo, Yogyakarta, Indonesia. Intl J Bonorowo Wetlands 13: 1-8. The mangrove forest ecosystem is a forest with coastal plant characteristics that are influenced by tides and tolerate high salt levels. Mangrove forests in Indonesia contain about 89 species of flora, and around 18% of their fauna are keystone species. Keystone species are species that play an important role in maintaining the balance of the ecosystem. Austruca annulipes (H. Milne-Edwards, 1837), or Ring-legged fiddler crab is one of the fauna species in the mangrove ecosystem, which has an important role in maintaining the balance of the food chain and nitrogen cycle, so the study of this species is very important for the sustainability of the ecosystem in mangrove area. This study aimed to determine the population density and distribution patterns of A. annulipes in the Mangrove Bogowonto Estuary Area, Kulon Progo, Yogyakarta, Indonesia. The research method was purposive random sampling using a 10m x 10m square plot. Furthermore, the identification of morphology and documentation of the activities of A. annulipes was carried out, and data were examined using calculations of crab density. Based on the research finding, the population density of A. annulipes is 0.9975 individuals/m2. Calculating the Morisita Index of A. annulipes obtained a value of 1.38 with the category of clumped distribution patterns. Various factors influence this distribution, including abiotic factors such as soil moisture, temperature, air, salinity, and pH. In addition, the presence of vegetation has also affected the density of A. annulipes because it is used as a shelter and foraging among its roots.

Plant Growth Promoting Rhizobacteria in Plant Health: A Perspective Study of the Underground Interaction.

Plants are affected by various environmental stresses such as high or low temperatures, drought, and high salt levels, which can disrupt their normal cellular functioning and impact their growth and productivity. These stressors offer a major constraint to the morphological, physiological, and biochemical parameters; thereby attributing serious complications in the growth of crops such as rice, wheat, and corn. Considering the strategic and intricate association of soil microbiota, known as plant growth-promoting rhizobacteria (PGPR), with the plant roots, PGPR helps plants to adapt and survive under changing environmental conditions and become more resilient to stress. They aid in nutrient acquisition and regulation of water content in the soil and also play a role in regulating osmotic balance and ion homeostasis. Boosting key physiological processes, they contribute significantly to the alleviation of stress and promoting the growth and development of plants. This review examines the use of PGPR in increasing plant tolerance to different stresses, focusing on their impact on water uptake, nutrient acquisition, ion homeostasis, and osmotic balance, as well as their effects on crop yield and food security.

Salt-induced phosphoproteomic changes in the subfornical organ in rats with chronic kidney disease

Objectives Subfornical organ (SFO) is vital in chronic kidney disease (CKD) progression caused by high salt levels. The current study investigated the effects of high salt on phosphoproteomic changes in SFO in CKD rats. Methods 5/6 nephrectomized rats were fed a normal-salt diet (0.4%) (NC group) or a high-salt diet (4%) (HC group) for three weeks, while sham-operated rats were fed a normal-salt diet (0.4%) (NS group). For phosphoproteomic analysis of SFO in different groups, TiO2 enrichment, isobaric tags for relative and absolute quantification (iTRAQ) labeling, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used. Results There were 6808 distinct phosphopeptides found, which corresponded to 2661 phosphoproteins. NC group had 168 upregulated and 250 downregulated phosphopeptides compared to NS group. Comparison to NC group, HC group had 154 upregulated and 124 downregulated phosphopeptides. Growth associated protein 43 (GAP43) and heat shock protein 27 (Hsp27) were significantly upregulated phosphoproteins and may protect against high-salt damage. Differential phosphoproteins with tight functional connection were synapse proteins and microtubule-associated proteins, implying that high-salt diet disrupted brain’s structure and function. Furthermore, differential phosphoproteins in HC/NC comparison group were annotated to participate in GABAergic synapse signaling pathway and aldosterone synthesis and secretion, which attenuated inhibitory neurotransmitter effects and increased sympathetic nerve activity (SNA). Discussion This large scale phosphoproteomic profiling of SFO sheds light on how salt aggravates CKD via the central nervous system.

Multi-elemental analysis of free water obtained from water in oil emulsions from Niger Delta Region using ICP-OES

Disposal of produced water obtained from crude oil could be challenging and tough because of the effect it has on the environment. Produced water contains high levels of salt and toxic substances which must be treated before re-use or disposal to reduce their impact on the environment, people and assets. Hence, the characterization of produced water for its chemical composition is essential. This study presents the determination of heavy metals (Co, Cu, Pb, Fe, Zn, Ni, and Cd) in produced water obtained from crude oil emulsion samples via inductively coupled plasma optical emission spectrometry (ICP-OES). The samples were subjected to treatment prior to analysis. The analytical curve obtained from the calibration gave excellent correlation coefficients ranging from 0.9995- 1.000 and the recovery values obtained were very good (95% - 105%). The result of the heavy metal analysis in μg/l (Co: <DL to 14.3, Cu: 114.1 to 1553.2, Fe: 492.1 to 1576.4, Ni: 52.3 to 174.5, Pb: <DL to 15.1, Zn: 92.4 to 432.9, Cd: 2.3 to 4.4) indicates that the concentrations of the metals evaluated in the produced water samples were above the recommended discharge limit ( Zn - 1.0 mg/L, Co - 0.02 mg/L, Fe - 1.0 mg/L, Cu - 0.02 mg/L, Cd - 0.01 mg/L, Ni - 0.03 mg/L and Pb - 0.05 mg/L).

Using QuEChERS sample preparation technique followed by ICP-Ms method to determination Cr(III) and Cr(VI) in seawater

Currently, Cr(III) and Cr(VI) are analyzed directly in trace level using anion-exchange liquid chromatography inductively coupled plasma mass spectrometry (LC – ICP – MS), which is well-known as an expensive and complicated method. Therefore, determination of Cr(VI) and Cr(III) by spectrometer or combine with ICP (or AAS) is seemly prefer to choose in almost laboratories although this method presents a higher limit of detection (LOD) and is influenced by salt ions in seawater. In this study, after forming reddish-violet complex by adding diphenylcarbazide (DPC) to seawater samples, a QuEChERS extraction technique was applied to separate Cr from high salt level matrix and transfer the Cr(VI)-DPC structure to the acetonitrile phase. In case of determination of total Cr, the Cr(III) in samples had to convert to Cr(VI) by oxidate with KMnO4 before adding DPC. Then, a digestion process with HNO3 – H2O2 mixture was implemented before analyzing Cr(VI) by ICP-MS. The Cr(III) is calculated indirectly based on total Cr and Cr(VI) results. This method had a linear concentration range of Cr(VI) from 0.02 to 50 µg/L with a detection limit of 0.015µg/L, the relative standard deviations under 3,03%, and the recovery was 92-108 % for seawater. According to the outcome of parallel experiment with common method, which used spectrometer and did not apply QuEChERS extraction step, the ICP-MS method presented a better result with smaller LOD, RSD, and recovery range.