High Levels Of Iron Research Articles

Mechanisms and shapes of causal exposure-response functions for asbestos in mesotheliomas and lung cancers

This paper summarizes recent insights into causal biological mechanisms underlying the carcinogenicity of asbestos. It addresses their implications for the shapes of exposure-response curves and considers recent epidemiologic trends in malignant mesotheliomas (MMs) and lung fiber burden studies. Since the commercial amphiboles crocidolite and amosite pose the highest risk of MMs and contain high levels of iron, endogenous and exogenous pathways of iron injury and repair are discussed. Some practical implications of recent developments are that: (1) Asbestos-cancer exposure-response relationships should be expected to have non-zero background rates; (2) Evidence from inflammation biology and other sources suggests that there are exposure concentration thresholds below which exposures do not increase inflammasome-mediated inflammation or resulting inflammation-mediated cancer risks above background risk rates; and (3) The size of the suggested exposure concentration threshold depends on both the detailed time patterns of exposure on a time scale of hours to days and also on the composition of asbestos fibers in terms of their physiochemical properties. These conclusions are supported by complementary strands of evidence including biomathematical modeling, cell biology and biochemistry of asbestos-cell interactions in vitro and in vivo, lung fiber burden analyses and epidemiology showing trends in human exposures and MM rates.

Phytoaccumulation of trace elements (As, Cd, Co, Cu, Pb, Zn) by Nicotiana glauca and Euphorbia segetalis growing in a Technosol developed on legacy mine wastes (Domingo Rubio wetland, SW Spain)

Sulfidic mine wastes have the potential to generate acid mine drainage (AMD) and release acid leachates containing high levels of iron, sulfate and potentially toxic elements (PTEs). Soils receiving AMD discharges are generally devoid of vegetation. Only a few metal-tolerant plant species can survive under such adverse soil conditions. This work investigates two plant species, Nicotiana glauca and Euphorbia segetalis, that have successfully colonized an AMD-impacted wetland area in south-western Spain. The uptake of PTEs from the soil by roots and their transfer and accumulation in the above-ground biomass were quantified. Results showed that these pioneer plants grew in patches of neutral soil within the wasteland despite the high concentrations of PTEs in the rhizosphere soil (up to: 613 mg kg−1 As, 18.7 mg kg−1 Cd, 6370 mg kg−1 Cu, 2210 mg kg−1 Pb and 5250 mg kg−1 Zn). The target organs of As, Cu and Pb accumulation were: root > leaf > stem in N. glauca, and root > stem > leaf in E. segetalis. Zinc and Cd showed a significant decrease in roots relative to aerial parts of N. glauca, and Co was preferentially partitioned in stems of N. glauca and leaves of E. segetalis. The soil–plant transfer coefficient values of PTEs in all parts of both plants were well below unity with the only exception of Cd in leaves of N. glauca (1.254), suggesting that roots acted as a barrier limiting the uptake of PTEs by plants. Interestingly, under the same soil conditions, N. glauca absorbed Cd in considerable proportions from soil and accumulated it in its leaves, while E. segetalis was not effective in transferring PTEs from roots shoots except for Co. In conclusion, soil pH and plant-related factors greatly influence the stabilization of PTE in the rhizospheric soil and produce inconsistencies in PTE phytoavailability. The findings of this study provide criteria to assist in natural remediation in other legacy contaminated sites worldwide.

Effect of coffee husk and cocoa pods biochar on phosphorus fixation and release processes in acid soils from West Cameroon

AbstractAcid soil in West Cameroon has limited phosphorus (P) availability which limits plant growth. This is mainly because of low pH, high levels of exchangeable aluminium (Al) and iron (Fe) and fixation of P. In this study, acid soils, sampled in Bafang, were amended with biochar produced from coffee husks (CH) and cocoa pod husks (CP) at two different temperatures (350 and 550 °C) in other to evaluate the effect on the physicochemical properties of the acid soil and the effect on P sorption and desorption. The soil was amended with biochar at a rate of 0, 20, 40 and 80 g/kg and incubated for 7 and 60 days. Physicochemical properties of all soil–biochar samples were determined followed by sorption experiments and data fitted in the Langmuir and Freundlich isotherm models in other to evaluate soil P sorption capacity and its affinity to soil amended with biochar. Moreover, desorption studies were done to evaluate the availability of P in soil amended with biochar after sorption. The outcomes of this study reveal an increase in soil pH, electrical conductivity (EC), available P, soil organic carbon and a drastic decrease in exchangeable Al and Fe. The point of zero charge of biochar‐amended soil was higher than the control and increased with amendment rate. The experimental data of the sorption of P on soils and soil–biochar samples fits into Langmuir and Freundlich models (R2 > 0.9) suggesting that the P adsorption is controlled by both model mechanisms. Soil–biochar mixture results in a decrease in the sorption capacity as compared with the control and the decrease was predominant with increasing amendment rate. At amendment rates of 20, 40 and 80 g/kg after 7 days of incubation, for SCH350 were 2267, 2048 and 1823 mg/kg which increased to 2407, 2112 and 1990 mg/kg after 60 days of incubation. This tendency was observed for all biochar inputs with respect to the increase in incubation days. Furthermore, desorption of P from soil–biochar mixtures was enhanced with biochar added at greater rate and produced at higher temperature. The desorption percentage was increased by more than around 10% for all biochar types from 20 mg/kg to 80 mg/kg amendment. Thus, biochar addition to acid soils reduces P fixation to acid soil and improves P desorption to soil solution, thereby providing more available P in the soil solution and better conditions for plant growth.

A multi-millennial record of rock glacier ice chemistry (Lazaun, Italy)

Active rock glaciers—known as mixtures of unconsolidated debris with interstitial ice, ice lenses or a core of massive ice—are widespread indicators of mountain permafrost. The age of a frozen rock glacier core in the Central European Alps (Lazaun, Italy) was dated to about 10,000 years. Here we report on the chemical composition of the frozen Lazaun core. The ice containing part of the core extended from about 2.8 m down to 24 m depth and consisted of two lobes—both a mix of ice and debris, separated by more than 3 m thick almost ice-free layer. The two lobes of the core showed layers of high solute content and peak values of electrical conductivity exceeding 1,000 μS/cm, but they differed in acidity and metal concentration. High acidity (minimum pH of 4.15) and high levels of elements like nickel, cobalt, zinc, manganese, iron and aluminum characterized the upper lobe, while neutral to alkaline pH and low metal values prevailed in the bottom lobe. We attributed solutes accumulated in the ice matrix to the weathering of bedrock minerals, with peak values favored by the oxidation of pyrite, or by an enhanced reactive surface area in fine-grained sediment layers. The chemical composition of the ice core also revealed signals of prehistoric atmospheric deposition from different sources including wood combustion, metal ore mining, and large volcanic eruptions (Thera, Aniakchak II). To our knowledge, this is the first study that presents the chemical stratigraphy of an entire rock glacier ice core.

Impact of inorganic iron and haem on the human gut microbiota; An in vitro batch-culture approach.

Although iron is an essential nutrient for humans, as well as for almost all other organisms, it is poorly absorbed (~15%) from the diet such that most passes through the upper gut into the large intestine. The colonic microbiota is thus exposed to, and potentially influenced by, such residual iron which could have an impact on human health. The aim of the research described here is to determine how the major forms of dietary iron (inorganic iron and haem) influence metabolic activity and composition of the human gut microbiota by utilizing an in vitro parallel, pH-controlled anaerobic batch culture approach. Controlled iron provision was enabled by the design of a 'modified' low-iron gut-model medium whereby background iron content was reduced from 28 to 5 μM. Thus, the impact of both low and high levels of inorganic and haem iron (18-180 μM and 7.7-77 μM, respectively) could be explored. Gut-microbiota composition was determined using next generation sequencing (NGS) based community profiling (16S rRNA gene sequencing) and flow-fluorescent in situ hybridization (FISH). Metabolic-end products (organic acids) were quantified using gas chromatography (GC) and iron incorporation was estimated by inductively coupled plasma optical emission spectroscopy (ICP-OES). Results showed that differences in iron regime induced significant changes in microbiota composition when low (0.1% w/v) fecal inoculation levels were employed. An increase in haem levels from 7.7 to 77 μM (standard levels employed in gut culture studies) resulted in reduced microbial diversity, a significant increase in Enterobacteriaceae and lower short chain fatty acid (SCFA) production. These effects were countered when 18 μM inorganic iron was also included into the growth medium. The results therefore suggest that high-dietary haem may have a detrimental effect on health since the resulting changes in microbiota composition and SCFA production are indicators of an unhealthy gut. The results also demonstrate that employing a low inoculum together with a low-iron gut-model medium facilitated in vitro investigation of the relationship between iron and the gut microbiota.

A pilot study to evaluate clinical factors associated with iron and ferritin elevations during pediatric extracorporeal membrane oxygenation.

Introduction: Elevations in serum ferritin and serum iron occur during pediatric extracorporeal membrane oxygenation (ECMO). Previous reports attribute the elevation to frequent red blood cell transfusions and/or hemolysis. Chronic transfusion can cause iron deposition in tissues leading to multisystem organ dysfunction. This study aims identify clinical factors associated with elevated ferritin and iron in pediatric ECMO patients, along with post-decannulation magnetic resonance imaging (MRI) assessment of iron deposition in liver and brain.Methods: Prospective, pilot study, using descriptive statistics to investigate potential associations between patient characteristics, serum ferritin and iron levels, and post-decannulation hepatic and basal ganglia iron deposition.Results: In this study, nine patients (100%) had elevated serum ferritin levels during ECMO. High ferritin levels were more common with veno-arterial than with veno-venous cannulation (p = 0.026) and were also associated with high plasma free hemoglobin levels (p < 0.001). Five patients presented with elevated serum iron levels. High serum iron levels were associated with higher daily (p = 0.016) and cumulative transfusion volumes (p = 0.013) as well ECMO duration beyond 7days. MRI scans were performed on three patients with no evidence of abnormal iron deposition detected in the liver or brain.Conclusions: This pilot study shows that during pediatric ECMO, elevations in serum ferritin and serum iron occur and those elevations may be related to the cannulation modality, ECMO duration, amount of hemolysis, and volume of red blood cell transfusions. Further investigation is warranted to fully understand the implications of elevated serum iron and ferritin in pediatric ECMO.

Predictive value of ferroptosis-related biomarkers for diabetic kidney disease: a prospective observational study

AimsTo explore the predictive value of ferroptosis-related (FR) biomarkers for diabetic kidney disease (DKD) in patients with type 2 diabetes mellitus (T2DM).MethodsThis prospective observational study enrolled patients with T2DM at the Second Hospital of Jilin University between December 2021 and March 2022. DKD was measured by the urinary albumin-to-creatinine ratio. Receiver operating characteristic curve (ROC) analysis was performed to assess the predictive value of ferroptosis-related biomarkers for DKD.The risk factors for massive proteinuria were performed by multivariable logistic regression analysis.ResultsFinally, 118 patients (53.0 ± 12.2 years, 76 males) were enrolled, 52 of them without DKD (had normal proteinuria), while 66 with DKD. (Forty-one had microproteinuria, and 25 had massive proteinuria.) FR biomarkers, including acyl-CoA synthase long chain family member 4 (ACSL4), malondialdehyde (MDA), and reactive oxygen species (ROS), were significantly higher in the massive proteinuria group than in the other groups, while glutathione peroxidase 4 (GPX4) was significantly lower (all P < 0.05). The area under the ROC of the combination of GPX4, ACSL4, MDA, and ROS for predicting DKD was 0.804 (P < 0.001). Additionally, multivariate logistic regression analysis showed that the course of disease and ferritin levels were independent risk factors for massive proteinuria, while high serum iron, transferrin, and GPX4 levels were independent protective factors for massive proteinuria in patients with T2DM (all P < 0.05).ConclusionsThe GPX4, ACSL4, MDA, and ROS combination might have a good predictive value for DKD. Additionally, the course of disease, ferritin levels, serum iron, transferrin, and GPX4 were independently associated with massive proteinuria.

Operation of a high-flow UV-LED water treatment reactor with secondary effluent for stress testing

For various applications, including water disinfection, the ultraviolet light emitting diode (UV-LED) has recently emerged as a sustainable alternative to the conventional mercury lamp. We performed an accelerated life and stress testing field study of a custom-designed high-flow UV-LED drinking water disinfection reactor operating 24/7 with 20 L per minute (LPM) of extreme quality secondary effluent. The low UV transmittance (55–64 %) and high turbidity (4–10 NTU) considerably affected the performance of the system. Organic and inorganic fouling were observed on the reactor walls and protective quartz windows. However, the surface areas that were exposed to higher irradiance were found to be less prone to an organic fouling formation. Despite the low operating temperature of the UV-LEDs, the chemical deposition, the extent of which correlated with the UV irradiance profile, was observed over the quartz windows. This indicates the effect of UVC in promoting quartz scaling formation when the water contains high levels of calcium and iron. Unexpected levels of zinc in the fouling, despite its low concentration in the water, showed the importance of this element in the formation of fouling in the UV-LED water disinfection systems. After 22 days of field testing, the UV transmittance of the protective windows dropped by 50–60 %, which, along with the radiant power reduction of the UV-LEDs, resulted in a 67 % overall reduction in an equivalent dose of the reactor. The study showed that optimally designed drinking water UV-LED reactors can maintain notable performance, even after exposure to accelerated life testing in extreme water conditions. Furthermore, the fouling was reversible, and the reactor was easily cleaned using household cleaning solutions in a two-step process with bleach and CLR®, where the cleaned quartz had a UV transmittance equal to an unused quartz window.

The content of heavy metals in the habitat of birds, Zeya-Bureya plain

From 2019 to 2021, the content of heavy metals in the components of the aquatic ecosystem, which is the habitat of birds on the territory of the Zeya-Bureya plain, was studied (water, sediments, macrophytes, bird feathers). High levels of iron and manganese were found in the water of small rivers. Due to heavy rains and floods, heavy metals were washed out of the surface layers of soil in agricultural fields into rivers. High concentrations of copper and lead were found in the bottom sediments of small rivers. Agricultural pollution of aquatic ecosystems affected the accumulation of lead and manganese in macrophytes. Bird feathers contained the highest amounts of iron, zinc, copper, and manganese, and the lowest amounts of cobalt and cadmium. The feathers of protected bird species such as Swan Goose, Red-crowned Crane, Hooded Crane and Daurian white-naped Crane contained lead 13.5 -38.2 mg/kg, cadmium 0.17 - 0.51 mg/kg. The elements in the feathers of birds were of exogenous origin. Birds are bioindicators of anthropogenic pollution. Researches in this direction are necessary for an estimation of ecological risk of environment and acceptance of measures on its protection.

Seed bio-priming with phosphate-solubilizing bacteria strains to improve rice (Oryza sativa L. var. FARO 44) growth under ferruginous ultisol conditions.

This study investigated the possibility of using phosphate-solubilizing bacteria (PSB) with plant-growth-promoting (PGP) capabilities to improve the growth properties of rice plants under ferruginous ultisol (FU) conditions through bio-priming. The following PSB with PGP properties were used in this study: Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1, and Klebsiella variicola strain AUH-KAM-9, which were previously isolated and characterized based on 16S rRNA gene sequencing. Biosafety analysis of the PSB isolates was conducted using blood agar. The rice seeds were then bio primed with the PSB for 3, 12, and 24 h and then sown in a composite FU soil sample. Differences in germinat ion bioassay were investigated 15 weeks after bio-priming using scanning electron microscopy (SEM), morphology, physiology, and biomass parameters. The composite FU soil used in this study had high pH, low bioavailable phosphorus, low water-holding capacity, and high iron levels, which resulted in low growth properties of rice seeds without bio-priming in the FU soil. Germination parameters were improved in seeds bio primed with the PSB, especially after 12 h of priming, compared with seeds without priming. SEM showed higher bacterial colonization in bio primed seeds. Bio-priming of rice seeds with the studied PSB under FU soil conditions significantly improved seed microbiome, rhizocolonization, and soil nutrient properties, thereby enhancing the growth properties of rice. This indicated the ability of PSB to solubilize and mineralize soil phosphate and improve phosphorus availability and soil properties for optimum plant usage in phosphate-stressed and iron toxic soils.

Circulating Iron in Patients with Sickle Cell Disease Mediates the Release of Neutrophil Extracellular Traps

Introduction: Neutrophils promote chronic inflammation and release neutrophil extracellular traps (NETs) that can drive inflammatory responses. Inflammation influences progression of sickle cell disease (SCD), and a role for NETs has been suggested in the onset of vaso-occlusive crisis (VOC). We aimed to identify factors in the circulation of these patients that provoke NET release, with a focus on triggers associated with hemolysis. Methods: Paired serum and plasma samples during VOC and steady state of 18 SCD patients (HbSS/HbSβ⁰-thal and HbSC/HbSβ⁺-thal) were collected. Cell-free heme, hemopexin, and labile plasma iron have been measured in the plasma samples of the SCD patients. NETs formation by human neutrophils from healthy donors induced by serum of SCD patients was studied using confocal microscopy and staining for extracellular DNA using Sytox, followed by quantification of surface coverage using ImageJ. Results: Eighteen patients paired samples obtained during VOC and steady state were available (11 HbSS/HbSβ⁰-thal and 7 HbSC/HbSβ⁺-thal). We observed high levels of systemic heme and iron, concomitant with low levels of the heme-scavenger hemopexin in sera of patients with SCD, both during VOC and in steady state. In our in vitro experiments, neutrophils released NETs when exposed to sera from SCD patients. The release of NETs was associated with high levels of circulating iron in these sera. Although hemin triggered NET formation in vitro, addition of hemopexin to scavenge heme did not suppress NET release in SCD sera. By contrast, the iron scavengers deferoxamine and apotransferrin attenuated NET formation in a significant proportion of SCD sera. Discussion: Our results suggest that redox-active iron in the circulation of non-transfusion-dependent SCD patients activates neutrophils to release NETs, and hence, exerts a direct pro-inflammatory effect. Thus, we propose that chelation of iron requires further investigation as a therapeutic strategy in SCD.

Concentrações de microminerais (cobre, cobalto, ferro, molibdênio e zinco) no fígado de vacas leiteiras da Região de Campos Gerais, Paraná, Brasil

ABSTRACT: This study collected samples from 50 Holstein cows, most intensively bred, and from the Campos Gerais region, Paraná, with an average milk production of 30.21L/day. Samples of the liver, spleen and lymph nodes were collected to determine the levels of copper, cobalt, iron, molybdenum and zinc. Spleen and lymph nodes were subjected to histological analysis and evaluation of the degree of hemosiderosis. The average concentrations of copper (495.05ppm), molybdenum (4.19ppm), and zinc (274.49ppm) were higher than those established for the bovines. For cobalt 26% of the animals presented levels below the established level, which characterized cobalt deficiency. Only iron (299.12ppm) exhibited an adequate average level. Histopathologically hemosiderosis was observed mainly in the spleen (78%) and less frequently in the lymph nodes (20%). The observation of hemosiderin in the spleen and lymph nodes is not related to copper deficiency. Still it may be related to high levels of molybdenum, zinc, iron, or other undetermined causes.

Comparisons of microstructure and elemental composition of eggshells among wild plover populations

Reproduction investment is a prominent trade-off in life-history theory and is subject to strong selection pressure. The avian eggshell, as a crucial barrier between the bird embryo and the surrounding environment, undergoes optimization under different environmental selection regimes to ensure the successful development of embryos, which can be linked to local adaptation. Therefore, understanding the variation in eggshell microstructure and composition in wild bird populations living in contrasting ambient environments is of great significance. In this study, we utilized electron microscope ultrastructure measurement and elemental analyses to measure and compare the microstructure and element composition of eggshells from three wild plover populations (Charadrius alexandrinus and C. dealbatus) residing in heterogeneous habitats across varied climatic zones. These populations include the high-altitude Qinghai Lake population, the temperate coastal Tangshan population, and the tropical coastal Zhanjiang population. Our findings revealed that the palisade layer was thinner in the Qinghai Lake population compared to its lowland populations. This difference might be attributed to hypoxia which facilitates the hatching process by allowing chicks to easily break through their shells. Additionally, the variations in the elemental composition of the eggshells among populations well reflected the distribution of element content in different geographical regions. The Qinghai Lake population displayed low zinc and low manganese levels but high calcium levels, while the Zhanjiang population exhibited high zinc, high iron, high manganese, and high phosphorus levels. Furthermore, these variations in elemental composition could also account for the observed microstructural differences among populations. Collectively, we propose that the dissimilarities in eggshell microstructure and elemental composition among populations could be attributed to adaptations to different environmental conditions. Our findings lay the groundwork for future research to explore the mechanisms behind the variations in eggshell characteristics among wild bird populations, and contribute to a broader understanding of biodiversity mechanisms.

Case of Vibrio Vulnificus bacteremia in a patient heterozygous for HFE p.C282Y mutation and alcoholic liver cirrhosis

Vibrio vulnificus is a Gram-negative bacterium, a member of the Vibrionaceae family. V. vulnificus is the main cause of seafood-related deaths in the United States because it can cause severe wound infections or sepsis. This microorganism is highly dependent on iron availability. Therefore, patients with high body iron levels are more susceptible to the infection. Prompt treatment with cephalosporins as well as doxycycline is usually administered. We present a case of V. vulnificus bacteremia in a patient heterozygous for HFE p.C282Y mutation and underlying alcoholic liver cirrhosis.

Cyanogenic Glycoside Reduction of Bamboo Shoots Species and Their Prospective as Food Victuals

Bamboo encompasses a diverse array of bioactive compounds along with cyanogenic glycoside (taxiphyllin) that has deleterious impacts on human health. In the present study, an assessment of the nutritional and antioxidant profiles has been evaluated for two species of bamboo shoots and a diverse array of processing techniques were adapted to mitigate the presence of harmful taxiphyllin. The results for proximate parameters showed that Bambusa vulgaris had high levels of protein (4.41g/100g) and fiber (3.81g/100g) whereas,Dendrocalamushamiltonii exhibits high levels of iron (2.45mg/100g), calcium (35.12mg/100g), and phosphorus (38.14mg/100g). The phytochemical analysis of Bambusa vulgaris and Dendrocalamushamiltonii revealed the total phenolic content 38.04±0.7 mgGAE/g and the total flavonoid content 46.12±0.05 mgQE/g was found to be higher to that ofBambusavulgaris.Furthermore, processing methods aimed the degradation of cyanogenic glycoside content in both bamboo shoot species which were 512.43 mg/kg and 1180.15 mg/kg in Bambusa vulgaris andDendrocalamushamiltoniirespectively, involving a sequence of processing techniques such as boiling, soaking, steaming, and blanching at various durations to attain optimal cyanogenic glycoside elimination. The process of blanching bamboo shoots in a 1% acetic acid solution for a period of 2 minutes was determined to be the most efficient technique for both species and recorded the reduction up to 0.04 mg/kg. The present research aims to emphasize the potential of two species of young bamboo shoots, as food victuals, and its efficacy as a nutritious food source that is lacking awareness due to an insufficient knowledge among common people.

Sodium para-aminosalicylic acid ameliorates brain neuroinflammation and behavioral deficits in juvenile lead-exposed rats by modulating MAPK signaling pathway and alpha-synuclein

Lead (Pb) is a developmental neurotoxin that can disrupt brain development and damage the brain regions responsible for executive function, behavioral regulation and fine motor control. Sodium para-aminosalicylic acid (PAS-Na) is a non-steroidal anti-inflammatory drug that can cross the blood-brain barrier. The purpose of this study was to examine the effects of juvenile rat Pb exposure on behavioral changes and brain inflammation, and the efficacy of PAS-Na in ameliorating these effects. The results showed that Pb exposure during the juvenile period (from weaning to adult period) delayed rats’ growth development and impaired their motor learning. Pb exposure not only increased Pb concentrations in several brain regions (including hippocampus, striatum and substantia nigra), but also disrupted metal-homeostasis in the brain, as higher levels of iron (Fe) and calcium (Ca) were observed in the substantia nigra. Moreover, Pb activated the MAPK pathway and increased levels of inflammatory factors such as IL-1β, TNF-α and IL-6 in the hippocampus, striatum and substantia nigra. Furthermore, Pb increased the levels of alpha-synuclein (α-syn) in these brain sites. PAS-Na improved the motor deficits and brain inflammation in the Pb-exposed rats. Moreover, the elevated Pb, Fe and Ca concentrations in the brain were significantly reduced by PAS-Na, which contains amino, carboxyl and hydroxyl functional groups, suggesting that it may act as a chelator of brain metals. In addition, PAS-Na inhibited the Pb-induced MAPK pathway activation and α-syn accumulation in the same brain regions. Taken together, our novel study suggest that PAS-Na shows efficacy in improving the Pb-induced behavioral changes in rats by inhibiting MAPK-dependent inflammatory pathways and reducing α-syn accumulation.

Insights on Ferroptosis and Colorectal Cancer: Progress and Updates.

Patients with advanced-stage or treatment-resistant colorectal cancer (CRC) benefit less from traditional therapies; hence, new therapeutic strategies may help improve the treatment response and prognosis of these patients. Ferroptosis is an iron-dependent type of regulated cell death characterized by the accumulation of lipid reactive oxygen species (ROS), distinct from other types of regulated cell death. CRC cells, especially those with drug-resistant properties, are characterized by high iron levels and ROS. This indicates that the induction of ferroptosis in these cells may become a new therapeutic approach for CRC, particularly for eradicating CRC resistant to traditional therapies. Recent studies have demonstrated the mechanisms and pathways that trigger or inhibit ferroptosis in CRC, and many regulatory molecules and pathways have been identified. Here, we review the current research progress on the mechanism of ferroptosis, new molecules that mediate ferroptosis, including coding and non-coding RNA; novel inducers and inhibitors of ferroptosis, which are mainly small-molecule compounds; and newly designed nanoparticles that increase the sensitivity of cells to ferroptosis. Finally, the gene signatures and clusters that have predictive value on CRC are summarized.

Effects of Liming on Nodulation, Nitrogen Fixation and Seed Protein Content in Soybean

Soil acidity is influenced by high levels of aluminium, iron, manganese and low levels of Cation Exchange Capacity (CEC), plant nutrients availability such as phosphorus, affect the growth of symbiotic-fixing bacteria in soybean which is detrimental to nodule formation and functioning. In an attempt to evaluate liming effects on soil pH, nitrogen fixation and seed protein content, an experiment was set up using a randomized complete block design (RCBD) layout in a split-plot with four replications at the Crop Museum, the Sokoine University of Agriculture in Morogoro, Tanzania. Soybean genotypes (Bossier, Laela and Uyole soya-1) were used as the main plot, while lime levels (1560, 936 and 624 kg/ha) were used as the subplot. The analysis of variance (ANOVA) showed a significant influence of lime levels on soil pH, quantities of nitrogen fixed and seed protein content. There was a significant difference (P = 0.001) observed among soybean genotypes with the application of 1560 kg/ha of lime (pH 6.5), recording the highest (7.6) nodule counts and nodule dry weight (19.26). Among the different varieties used in the study, Bossier was observed to have a fixed 24.46 kgN/ha, while Laela produced the highest (10.60%) seed protein content. Application of 1560 kg/lime observed a significant increase in kgN/ha (35.71) as well as a minimal increase (15.66%) in seed protein content at pH 6.5. Interaction effects (P = 0.05), however, observed Laela has fixed the highest nodule (7.6) counts, nitrogen fixation (44.90 kgN/ha), with Bossier recording the highest (20.22%) seed protein. The study revealed that the physiochemical properties of acid soil, as well as soybean yield components such as nodules, N2 fixation and seed protein content, can be maximally improved when limed with CaCo3

Immunometabolic involvement of hepcidin genes in iron homeostasis, storage, and regulation in gilthead seabream (Sparus aurata)

Iron is essential for all living things, especially marine organisms, due to its low availability in the marine environment. Iron regulation is key in all vertebrates and is controlled by hepcidin–ferroportin. To improve the knowledge of iron homeostasis in fish, an iron overload was induced in gilthead seabream (Sparus aurata), which was chosen as a study species because of its high interest in Mediterranean aquaculture. The amount of iron in different tissues was measured to determine its biodistribution and/or bioaccumulation. Since the liver is directly involved in iron metabolism, the morphological changes induced in this organ as a consequence of the iron increase were studied. The bactericidal activity of fish skin mucus was also determined, observing that it decreased in fish with high iron levels compared to control fish. In addition, to better understand iron regulation, the gene expression of hepcidin, ferroportin, transferrin, and ferritin was evaluated in the head kidney (the main hematopoietic organ in this species) and in the liver. Special interest was taken in the study of the multiple copies of the hamp2 gene present in the gilthead seabream genome. Bioinformatic analysis of the protein sequences derived from these hepcidin genes allowed us to determine the presence of one type I hepcidin and 12 type II hepcidins, all of them with antimicrobial potential. This number of mature hepcidin sequences found in gilthead seabream is the highest within Eupercaria described to date. All the results obtained indicate that the modulation of iron in seabream seems to be much more complicated than in other vertebrates, probably due to the possible involvement of the different hepcidins as mediators between iron metabolism and host immune response.

Co-occurrence of antimicrobials and metals as potential drivers of antimicrobial resistance in swine farms

The application of animal manures to cropland is an important nutrient recycling strategy in many parts of the world. Commonly, aggregated manure wastes contain chemical stressors including veterinary antimicrobials, heavy metals, and antimicrobial resistance genes (ARGs) that can stimulate the development and proliferation of antimicrobial resistance (AMR). While the presence of antimicrobials in manure is well-documented, the co-occurrence of other potentially impactful chemical stressors in swine manure remains underreported. This study quantifies and analyzes correlations between antimicrobials, metals, and certain ARGs present in manure samples from swine farms in Iowa, United States. Relationships between chemical stressors and different stages of swine production or feed composition are also investigated. Results revealed substantial levels of tetracyclines [up to 1,260 µg g−1 dry weight (d.w.) of manure for oxytetracycline] detected in all samples. Tiamulin, two ionophores (monensin and lasalocid), and one macrolide (tilmicosin) were detected at maximum class concentrations of 9.4, 0.547, and 0.472 µg g−1 d.w., respectively. The median relative abundances of ermB and tetM were 0.13 and 0.17 copies g−1 wet weight (w.w.) manure (normalized to 16S gene), respectively. Additionally, high levels of copper (Cu), iron (Fe), and zinc (Zn) were detected in all samples, with maximum concentrations of 887, 1,900, and 2,100 µg g−1 d.w., respectively. Notably, uranium (U) was detected in 11 samples, at concentrations up to 0.77 µg g−1. A global analysis of AMR-stressor relationships using Spearman’s rank correlation indicates Cu, and Ba are the most positively and significantly correlated with cytotoxic anhydrotetracycline (ATC) and/or anhydrochlortetracycline (ACTC) concentrations in all tested facilities (Cu-ATC: ρ = 0.67, p = 0.0093; Cu-ACTC: ρ = 0.75, p = 0.0022; Ba-ATC: ρ = 0.84, p = 0.0002). Interestingly, ermB and tetM genes were strongly, positively correlated to each other (ρ = 0.92, p &amp;lt; 0.0001), suggesting possible co-selection, despite the absence of correlation between ARGs and tetracycline concentrations. This study demonstrates the complexity of interactions between antimicrobials, metals, and ARGs in multiple manure storage pits prior to cropland application.