Herbicide-resistant Oilseed Rape Research Articles

Molecular characterization of imidazolinone-resistant Brassica rapa × B. napus hybrids.

Herbicide-resistant oilseed rape (Brassica napus) cultivation in our country entails the risk of gene transfer to related wild species. One of these species is the wild turnip (B. rapa), an important weed of winter crops widely distributed in the Pampas region. Despite hybridization risks, Clearfield ® oilseed rape is available in Argentina. In 2008, a B. rapa population, which was sympatric to an imidazolinone-resistant and a conventional oilseed rape cultivar, was located on a farm in the main cropping area of the country. Herbicide-resistant individuals were found in the progeny of this population in a herbicide screening test. Therefore, a molecular characterization using cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR) markers was conducted on these plants to determine their hybrid nature and to establish the origin of the imidazolinone resistance trait. The results of this study, along with information of field records, confirmed that the resistant plants were first generation interspecific hybrids. Imidazolinone resistance had been effectively transferred from the herbicide-resistant oilseed rape, even in the particular situation of pollen competition. Oilseed rape resistant cultivars are becoming more common in the country. So, considering that seed loss and crop volunteers are common in these species, it is crucial to avoid the dispersion of new resistant weed biotypes as they reduce the effectiveness of chemical control technologies.

Assessment of new scientific elements supporting the prolongation of prohibition of the placing on the market of genetically modified oilseed rape GT73 for food and feed purposes in Austria

Assessment of new scientific elements supporting the prolongation of prohibition of the placing on the market of genetically modified oilseed rape GT73 for food and feed purposes in Austria

Assessment of new scientific elements supporting the prolongation of prohibition of the placing on the market of genetically modified oilseed rape Ms8, Rf3 and Ms8×Rf3 for food and feed purposes in Austria

Assessment of new scientific elements supporting the prolongation of prohibition of the placing on the market of genetically modified oilseed rape Ms8, Rf3 and Ms8×Rf3 for food and feed purposes in Austria

Long-Term Monitoring of Field Trial Sites with Genetically Modified Oilseed Rape (Brassica napus L.) in Saxony-Anhalt, Germany. Fifteen Years Persistence to Date but No Spatial Dispersion.

Oilseed rape is known to persist in arable fields because of its ability to develop secondary seed dormancy in certain agronomic and environmental conditions. If conditions change, rapeseeds are able to germinate up to 10 years later to build volunteers in ensuing crops. Extrapolations of experimental data acted on the assumption of persistence periods for more than 20 years after last harvest of rapeseed. Genetically-modified oilseed rape—cultivated widely in Northern America since 1996—is assumed not to differ from its conventional form in this property. Here, experimental data are reported from official monitoring activities that verify these assumptions. At two former field trial sites in Saxony-Anhalt genetically-modified herbicide-resistant oilseed rape volunteers are found up to fifteen years after harvest. Nevertheless, spatial dispersion or establishment of GM plants outside of the field sites was not observed within this period.

Fixed-route monitoring and a comparative study of the occurrence of herbicide-resistant oilseed rape (Brassica napus L.) along a Japanese roadside

ABSTRACTPreviously, we conducted a roadside survey to reveal the occurrence of genetically modified (GM) oilseed rape along a Japanese roadside (Route 51). In this study, we performed successive and thorough fixed-route monitoring in 5 sections along another road (Route 23). Oilseed rape plants were detected on both sides of the road in each section between autumn 2009 and winter 2013, which included 3 flowering seasons. In four sections, more plants were found on the side of the road leading from the Yokkaichi port than on the opposite side. In the fifth section, the presence of clogged drains on the roadside, where juvenile plants concentrated, caused the opposite distribution: oilseed rape predominantly occurred along the inbound lanes (leading to the Yokkaichi port) in 2010 and 2012. Unlike in our previous survey, glyphosate- or glufosinate-resistant oilseed rape plants were abundant (>75% of analyzed plants over 3 years). Moreover, a few individuals bearing both herbicide resistance traits were also detected in some sections. The spillage of imported seeds may explain the occurrence of oilseed rape on the roadside. The abundance of herbicide-resistant oilseed rape plants may reflect the extent of contamination with GM oilseed rape seed within imports.

Backcrosses to Brassica napus of hybrids between B. juncea and B. napus as a source of herbicide-resistant volunteer-like feral populations

Introgression between genetically modified (GM) crops and wild relatives is considered to potentially modify the genetic background of the wild species. The emergence of volunteer-like feral populations through backcross of hybrids to the crop is also a concern. The progeny of spontaneous hybrids between mutant herbicide-resistant oilseed rape ( Brassica napus) and wild B. juncea was obtained. Parents, F 2 and BC 1 to B. napus were planted together in the field so as to study their performance. The chromosome number of BC 1 followed a Normal distribution. Mendelian ratio of the herbicide-resistance gene was found. The F 2 produced less seeds than B. napus, and BC 1 had intermediate production. Herbicide-resistant BC 1 were not different of their susceptible counterparts for plant weight, seed weight and seed number, but most of them exhibited B. napus morphology and larger flowers than the susceptible BC 1. They displayed an important genetic variability allowing further adaptation and propagation of the herbicide-resistance gene. Pollen flow to susceptible plants within the mixed stand was observed. As a consequence, the resistant BC 1 produced with B. napus pollen could frequently occur and easily establish as a false feral crop population within fields and along roadsides.

Are herbicide‐resistant crops the answer to controlling Cuscuta?

Herbicide-resistant crop technology could provide new management strategies for the control of parasitic plants. Three herbicide-resistant oilseed rape (Brassica napus L.) genotypes were used to examine the response of attached Cuscuta campestris Yuncker to glyphosate, imazamox and glufosinate. Cuscata campestris was allowed to establish on all oilseed rape genotypes before herbicides were applied. Unattached seedlings of C. campestris, C. subinclusa Durand & Hilg. and C. gronovii Willd. were resistant to imazamox and glyphosate and sensitive to glufosinate, indicating that resistance initially discovered in C. campestris is universal to all Cuscuta species. Glufosinate applied to C. campestris attached to glufosinate-resistant oilseed rape had little impact on the parasite, while imazamox completely inhibited C. campestris growth on the imidazolinone-resistant host. The growth of C. campestris on glyphosate-resistant host was initially inhibited by glyphosate, but the parasite recovered and resumed growth within 3-4 weeks. The ability of C. campestris to recover was related to the quality of interaction between the host and parasite and to the resistance mechanism of the host. The parasite was less likely to recover when it had low compatibility with the host, indicating that parasite-resistant crops coupled with herbicide resistance could be highly effective in controlling Cuscuta.

In-field frequencies and characteristics of oilseed rape with double herbicide resistance

When growing different transgenic herbicide-resistant oilseed rape cultivars side by side, seeds with multiple herbicide resistance can arise, possibly causing problems for the management of volunteer plants. Large-scale field experiments were performed in the years 1999/2000 and 2000/2001 in order to investigate the frequencies and the consequences of the transfer of herbicide resistance genes from transgenic oilseed rape to cultivars grown on neighboring agricultural fields. Transgenic oilseed rape with resistance to glufosinate-ammonium (LibertyLink, LL) and with glyphosate resistance (RoundupReady, RR), respectively, was sown in adjacent 0.5 ha plots, surrounded by about 8 ha non-transgenic oilseed rape. The plots and the field were either in direct contact (0.5 m gap width) or they were separated by 10 m of fallow land. Seed samples taken during harvest in the transgenic plots at different distances were investigated for progeny with resistance to the respective other herbicide. It was found that outcrossing frequencies were reduced to different extents by a 10 m isolation distance. In addition to pollen-mediated transgene flow as a result of outcrossing, we found considerable seed-mediated gene flow by adventitious dispersal of transgenic seeds through the harvesting machine. Volunteer plants with double herbicide resistance emerging in the transgenic plots after harvest were selected by suitable applications of the complementary herbicides Basta and Roundup Ultra. In both years, double-resistant volunteers were largely restricted to the inner edges of the plots. Expression analysis under controlled laboratory conditions of double-resistant plants generated by manual crosses revealed stability of transgene expression even at elevated temperatures. Greenhouse tests with double-resistant oilseed rape plants gave no indication that the sensitivity to a range of different herbicides is changed as compared to non-transgenic oilseed rape.

Monitoring the occurrence of genetically modified oilseed rape growing along a Japanese roadside: 3-year observations

Monitoring for escape of genetically modified (GM) oilseed rape (Brassica napus) during transport can be performed by means of roadside evaluations in areas where cultivation of this GM crop is not conducted, such as in Japan. We performed a survey of oilseed rape plants growing along a 20-km section of Japan's Route 51, one of the main land transportation routes in central Japan for imports of GM oilseed rape from the Port of Kashima into Keiyo District. Oilseed rape plants were found each year, but the number of plants varied substantially during the three years of our study: 2162 plants in 2005, 4066 in 2006, and only 278 in 2007. The low number in 2007 was probably caused by roadwork. Herbicide-resistant individuals were detected in the three consecutive years (26, 8, and 5 individuals with glyphosate resistance), but glufosinate-resistant plants (9 individuals) were detected only in 2005. The roadside plants occurred mainly along the inbound lane from Kashima to Narita. These plants are likely to have their origin in seeds spilled during transportation of cargo from the port, since there are no potential natural seed source plants for B. napus near Route 51. This is the first detailed report on the transition and distribution of herbicide-resistant oilseed rape plants following loss and spillage along Japanese roads.

Field study results on the probability and risk of a horizontal gene transfer from transgenic herbicide-resistant oilseed rape pollen to gut bacteria of bees

Bees are specifically subjected to intimate contacts with transgenic plants due to their feeding activities on pollen. In this study, the probability and ecological risk of a gene transfer from pollen to gut bacteria of bees was investigated with larvae of Apis mellifera (honeybee), Bombus terrestris (bumblebee), and Osmia bicornis (red mason bee), all collected at a flowering transgenic oilseed rape field. The plants were genetically engineered with the pat-gene, conferring resistance against glufosinate (syn. phosphinothricin), a glutamine-synthetase inhibitor in plants and microorganisms. Ninety-six bacterial strains were isolated and characterized by 16S rRNA gene sequencing, revealing that Firmicutes represented 58% of the isolates, Actinobacteria 31%, and Proteobacteria 11%, respectively. Of all isolates, 40% were resistant to 1 mM glufosinate, and 11% even to 10 mM. Resistant phenotypes were found in all phylogenetic groups. None of the resistant phenotypes carried the recombinant pat-gene in its genome. The threshold of detecting gene transfer in this field study was relatively insensitive due to the high background of natural glufosinate resistance. However, the broad occurrence of glufosinate-resistant bacteria from different phylogenetic groups suggests that rare events of horizontal gene transfer will not add significantly to natural bacterial glufosinate resistance.

GENE FLOW FROM TRANSGENIC GLUFOSINATE-OR GLYPHOSATE-TOLERANT OILSEED RAPE TO WILD RAPE

Aims Wild rape (Brassica juncea var. gracilis), a wild relative of oilseed rape (B. napus), was chosen for a case study because gene flow from herbicide-resistant oilseed rape to wild rape rarely has been explored. We asked a) are transgenic herbicide-resistant oilseed rape and wild rape sexually compatible, b) is the fitness of their F1 improved and c) is gene flow possible? Methods We used hand pollination to test sexual compatibility and examined resistance of the F1 by applying herbicide of 2 000 μg a.i.·kg-1 with 0.1 L·m-2 volume at 3-4 leaf stage twice and by PCR and examining vegetative growth, pollen viability and seed setting rate. Crossing from herbicide-resistant oilseed rape to wild rape also was tested under open pollination. Important findings Indexes of sexual compatibility of wild rape×glufosinate-tolerant oilseed and wild rape×glyphosate-tolerant oilseed were 13.87 and 13.83, respectively, under hand pollination, and these indexes were not significantly different from that of the female parent under self pollination and open pollination. All F1 were significantly resistant to the corresponding herbicide. Moreover, herbicide-resistant gene of F1 was confirmed by PCR and F1 of wild rape×glufosinate-resistant oilseed and wild rape×glyphosate-resistant oilseed produced 426 bp and 931 bp identical special band, respectively. The fitness of the F1 changed compared with that of the female parent. The germination rate of the F1 was similar with that of the female parent, 98%. Due to taller individuals and larger leaves, the vegetative growth of the F1 was much better than that of the female parent. However, pollen viabilities of both kinds of F1 were only half that of the female parent. Furthermore, the seed number per silique of the F1 was very low, only 0.59 and 0.58 seeds per silique, respectively. The cross rate of wild rape×glufosinate-resistant and wild rape×glyphosate-resistant was 0.02% and 0.01%, respectively, under open pollination. Our study suggests the possibility of gene flow from transgenic rape to wild rape. The possibilities of gene flow from the two herbicide-resistant transgenic oilseed rapes to wild rape were similar under hand pollination or open pollination. However, the effect of gene flow from transgenic herbicide-resistant oilseed to wild rape needs further research.

Transgenic oilseed rape along transportation routes and port of Vancouver in western Canada

The occurrence of transgenic herbicide-resistant oilseed rape (Brassica napus) in ruderal (non-crop disturbed) areas has not been investigated previously in Canada. The primary objective of this study was to document their occurrence in two main ruderal areas (along railways and roads) in the province of Saskatchewan, where half of all oilseed rape is grown, and at the port of Vancouver, British Columbia on the west coast of Canada, where most oilseed rape destined for export is transported by rail. During the 2005 growing season, leaf samples of oilseed rape plants were collected at randomly-selected sites along railways and roads across Saskatchewan ecoregions and at Vancouver; infestation area, density, and plant height of oilseed rape were measured at each site. The presence of the glyphosate and glufosinate resistance traits was determined using test strips. The infestation area of oilseed rape, averaged across 155 sampled sites in the Saskatchewan survey, was markedly smaller in populations along railways than roads; in contrast, infestation area averaged across 54 sites in the Vancouver survey was greater for populations along railways than roads. In both surveys, mean plant density was greater for populations found along railways than roads. Two-thirds of oilseed rape plants sampled across Saskatchewan ecoregions and at Vancouver were transgenic, although the relative proportion of plants with the glyphosate or glufosinate resistance trait varied between surveys. Frequency of occurrence of transgenic plants in ruderal areas was similar to the proportion of the oilseed rape area planted with transgenic cultivars in the recent preceding years. A single transgenic B. rapa x B. napus hybrid was found along a road in Vancouver, confirming the relatively high probability of hybridization between these two Brassica species. With current control measures, transgenic oilseed rape populations may persist and spread in these ruderal areas.