Bacterium Helicobacter Pylori Research Articles

Rise and fall of surgery for peptic ulcer disease

Earlier the treatment for peptic ulcer was surgery such as truncal vagotomy and drainage procedure. Recurrence after surgery and complications were common. It was because of our lack of knowledge about helicobacter pylori bacteria we used to blame factors such as lifestyle, other than infections causing peptic ulcer. It was due to two genius Australian doctors who identified the bacteria and the scenario was changed. Surgery for peptic ulcer gradually diminished dramatically after the development of H2 receptor antagonists and proton pump inhibitors. The surgery for complications of peptic ulcer such as perforations remain same but elective surgery for peptic ulcer drastically reduced. There was a time when after failed medical treatment for peptic ulcer patients were advised surgery or remain on cold milk and bread. These researches in medicine have alleviated human suffering from peptic ulcer disease. Patients of deep penetrating peptic ulcer used to weep with pain and treating clinicians also used to get frustrated for not getting ways to make patients painfree.

EXPERT SYSTEM FOR INITIAL IDENTIFICATION OF DISEASES CAUSED BY HELICOBACTER PYLORI BACTERIA USING CASE BASED REASONING APPROACH

Helicobacter pylori, which is a bacterium that can live in the stomach. Infection can occur when bacteria invade and damage the stomach wall. Lack of information and ignorance of the public about the seriousness of these bacteria causes various very serious diseases such as inflammation of the digestive tract (gastritis), gastric bleeding, gastric perforation (leak stomach), infection of the peritoneal wall (peritonitis) and gastric cancer. This expert system aims to provide information and also early identification of diseases caused by the bacterium Helicobacter pylori. After the expert system has identified the type of disease, it will then suggest the actions that need to be taken. The method used is CBR, this method works with the stages of Retrieve, Reuse, Revise, and Retain. The data that will be processed in this study are 30 data, with the results of 29 data in accordance with the doctor's diagnosis. From these results, it can be said that the accuracy of this expert system is 97% so that it can be used as an alternative in identifying diseases caused by the bacterium Helicobacter pylori.

Molecular and Genetic Study for Detection of Helicobacter pylori Virulence Genes among Patients with Diffuse Large B-Cell Lymphoma

Background: The bacteria Helicobacter pylori (Hp) has supposed to confirm an oncogenic consequence with the evolution of malignancy events in stomach, effectiveness of Hp consider a top risk factor of gastric-diffuse large B-cell lymphoma (DLBCL). Aims: the aim of this study was to observe the correlation between virulence factors, especially cytotoxin association gene A (CagA) and the progress of gastric DLBCL. Materials and Methods: Biopsy samples (50) involved in this study (28 were men, and 22 women, with a mean age up to 57.64 years), among gastric-DLBCL patients, with (25) control (were 14 men and 11 women) without DLBCL, tested at Medical City Teaching Hospital and Teaching Laboratories, study conducted from January 1st to September 30, 2022. Diagnosis was by molecular methods included DNA extraction for Hp by STR-Format kit (BIORON Diagnostics GmbHm In den Rauhweiden 20,67354 Germany), and “real-time polymerase chain reaction” methods using the (ABI PRISM 7700) Sequence Detection System, also applied the primers and probes, for Hp-CagA. Results: The mean of age was 55.7 ± 2 in DLBCL, while in control were 61.82 + 6, CagA +ve 66.66 + 2. In gastric DLBCL, the red blood cell count, white blood cell count, platelets count, and hemoglobin were all decline in DLBCL, not in the control group, lactate dehydrogenase levels in gastric DLBCL patients were rise (322 U/L), while less in control, while albumin (322g/dl) was less in gastric DLBCL patients. Hp 16S rRNA was detected relating to CagA variety, 66% (33/50) were CagA positive with highly significant in 23 patients with DLBCL (P = 0.004), and 10 in control patients. CagA gene expression was highly expression in gastric DLBCL patients with range up to (+), 8 (++), and 12 (+++), while in the control group was 5 (+), 2 (++), and 3 (+++), respectively. Conclusions: This present study fixed high frequency of CagA gene in gastric DLBCL patients. Moreover, confirmed this gene is significantly associated with gastric DLBCL in patients with Hp. Moreover, genotype EPIYA-ABC motif is related with the high risk of gastric DLBCL.

Single crystal structure feature and quantum mechanical studies of a new binuclear Bi (III) complex and its activity against Helicobacter pylori

• ● A new compound of Bi (III) has been synthesized. • ● The title complex crystallizes in a triclinic system with space group P-1. • ● Quantum mechanical studies were performed on the complex 1. • ● Complex 1 was assessed as a drug against three standard laboratory strains of H. pylori. • ● The complex showed excellent activity against H. pylori with MIC = 8.84 μM. A new Bi (III) coordination compound formulated as [Bi 2 (µ-ox)(dipic) 2 (taa) 4 ].2H 2 O ( 1 ) where ox 2- = oxalato, dipic 2- = pyridine 2,6- dicarboxylato and taa= thiourea has been synthesized. Single-crystal X-ray diffraction (SC-XRD) analysis has been carried out to determine the structure of the complex. From SC-XRD data, it was found that complex 1 is a di-nuclear compound and the title complex crystallizes in a triclinic system with space group P-1. The coordination around of Bi (III) center is distorted pentagonal-bipyramidal. Hirshfeld surface (HS) and two-dimensional fingerprint analysis were performed to investigate the interactions between the complex and the crystallized water molecules. The results showed that the O…H bonds are decisive with a contribution portion of 35.7%. Indeed, quantum mechanical studies were performed to understand effective factors on the stability of complex 1 . Complex 1 was assessed as a drug against three standard laboratory strains (26695, B128, and 251) of Helicobacter pylori ( H. pylori ). The results showed that the complex had excellent activity against the bacterium Helicobacter pylori with MIC = 8.84 μM.

Trace element levels in serum and gastric mucosa in patients with Helicobacter pylori positive and negative gastritis

BackgroundMost trace elements are inhibited by Helicobacter pylori-infection, and variations in specific element levels are linked to the development of stomach cancer. This is the first study to show the relationship between serum and tissue concentrations of twenty-five trace elements and H. pylori infection status. This study purposed to define serum and tissue trace element levels of 25 healthy individuals with Helicobacter pylori-positive gastritis and Helicobacter pylori-negative gastritis and to reveal their relationship with the disease. MethodsStudy groups consisted of sixty-two patients with Helicobacter pylori-positive, thirty-seven patients with Helicobacter pylori-negative, and thirty healthy individuals. Serum and tissue concentrations of twenty-five elements (aluminum, boron, arsenic, barium, calcium, beryllium, copper, cadmium, iron, chromium, mercury, lithium, potassium, magnesium, sodium, manganese, nickel, phosphorus, lead, scandium, strontium, selenium, tellurium, titanium, zinc) were defined by inductively coupled plasma optical emission spectrometry. ResultsExcept for copper, lithium, and strontium elements in serum samples, other trace elements differed significantly between the groups (p < 0.05). The serum chromium (p = 0.002), mercury (p = 0.001), boron (p < 0.001), and cadmium (p < 0.001) levels of H. pylori-negative gastritis and H. pylori-positive gastritis participants were significantly different, and their serum concentrations were less than 0.5 µ/l. Boron, barium, beryllium, chromium, lithium, phosphorus and strontium elements in tissue samples did not differ significantly between the groups (p > 0.05). Manganese, nickel, tellurium and titanium elements were not detected in tissue and serum samples. The mean concentrations of calcium, beryllium, chromium, iron, potassium, lithium, magnesium, scandium, and selenium were higher in the tissues of patients with H. pylori gastritis compared to healthy control tissues. Also, cadmium could not be detected in tissue samples. There was a significant difference between H. pylori-infected tissue and serum chromium levels (p = 0.001), with lower levels detected in tissue samples. ConclusionThis is the first study that we are knowledgeable of that reports the concentrations of twenty five elements in both serum and tissue samples, as well as the relationship between trace elements and Helicobacter pylori-infection status. Dietary adjustment is indicated as an adjunct to medical therapy to stabilize trace elements because Helicobacter pylori bacteria cause inflammation and impair element absorption in gastritis patients. We also think that this study will shed light on studies on the relationship between Helicobacter pylori-trace elements and serum-tissue/healthy serum-tissue trace element levels of patients with Helicobacter pylori gastritis.

The role of highly toxigenic CagA and VacA strains of Helicobacter pylori in gastric carcinogenesis. Review

Gastric cancer takes the 4th place as the main cause of death among oncological diseases in the world. The risk factors of gastric cancer include genetic predisposition, alcohol abuse, smoking, low socio‑economic level, nutritional characteristics, Epstein–Barr virus and Helicobacter pylori infection. Helicobacter pylori bacterium is the only statistically proven risk factor for stomach cancer. Gastric cancer develops in less than 3 % of ­H. pylori ‑infected patients, suggesting a unique interaction between the host immune response and H. pylori virulence factors. Expression of oncogenic cytotoxin‑associated protein gene A (CagA), vacuolating cytotoxin A (VacA), and outer membrane proteins is important for H. pylori in host pathogenesis. The paper gives consideration for the relationship between the virulence factors of H. pylori and the host, which is necessary for a better understanding of the pathogenesis and prevention of carcinogenesis. The strategy for the prevention of gastric cancer includes eradication therapy, but it is complicated by the growth of resistance to antimicrobial drugs. Only insignificant number of papers investigate effects of pathogenicity factors of H. pylori (CagA+ and VacA+) on the development of atrophy, intestinal metaplasia and dysplasia. In the literature, there is little data on the difference in antibiotic resistance between H. pylori highly toxigenic and non‑toxigenic strains. The aim of the article is to present current data on the impact of highly toxigenic CagA+VacA+ strains of H. pylori on the processes of gastric carcinogenesis and to determine their role in resistance to clarithromycin. Investigation of the relationship between highly toxigenic CagA and VacA strains with clinical and morphological manifestations of gastritis and antibiotic resistance will enable preventing the progression of precancerous changes and using effective treatment tactics.

Some Azo Compounds Containing Black Tea Processing Waste Catechins As Antioxidant and Urease Enzyme Inhibitory

Although various azo compounds containing some natural origin catechins had been synthesized and determined their dyeing properties for various textile products, azo compounds containing black tea waste catechins and their antioxidant capacity and urease enzyme inhibition were not investigated until this study. The urease enzyme is the most important enzyme that allows the bacteria Helicobacter pylori, which is considered the main factor of stomach cancer, to live in the stomach. Inhibition of this enzyme is very important for the treatment of Helicobacter pylori infection. It has been known that catechin extracts of natural origin inhibit the urease enzyme of Helicobacter pylori from literature. Black tea processing waste is a residue that is separated from the sieves during tea processing and has no economic value. The transformation of this residue into products that produce added value is very important because it contains many chemicals contained in the tea plant. &#x0D; In this study, some azo compounds containing black tea processing waste catechins were synthesized and investigated their antioxidant capacity, urease enzyme inhibition properties.

Antimicrobial evaluation of the Cynara cardunculus extract in Helicobacter pylori cells using mid-infrared spectroscopy and chemometric methods

A preliminary assay, using both therapeutic-based antibiotics (clarithromycin and metronidazole), was conducted to optimize experimental conditions in terms of the sensibility of the Fourier-transform mid-infrared (MIR-FTIR) spectroscopy associated with chemometric methods. Principal component analysis was applied to understand how the Cynara extract concentration acts differentially against H. pylori bacteria. The partial least squares model, characterized by R2 =0.98, and root mean square error cross-validation, 0.011, was developed for the spectral regions (3600-2500 cm-1 and 2000-698 cm-1 ). MIR-FTIR spectroscopy associated with chemometric methods can be considered a suitable approach to discover and analyse the promissory antimicrobial agents based on the biomolecular changes observed according to the Cynara extract. MIR-FTIR spectroscopy and chemometric methods allowed to register the biomolecular changes due to the potential antimicrobial drugs at reduced concentrations comparatively to the conventional assay based on an agar-dilution method, being considered a useful approach to develop a platform to discover new bioactive molecules, allowing to reduce time and costs related to the exploratory step.

The Prevalence of Helicobacter pylori Infections among Blood Donors in Blood Bank of Zawia Teaching Hospital and the Assessment of Some Related Factors

Infection by the bacterium Helicobacter pylori is transmissible and is considered a public health issue which affects people of all ages. This study aims to investigate the prevalence of H. pylori infections among blood donors and to assess the correlation of different lifestyles with H. pylori infections in addition to considering the prevalence of infections among blood Donor in regards to age. This study was conducted at the blood bank at Zawia Hospital, Libya, from the first of March until the end of May 2021. One hundred male donors aged between 22-61years were included in the study. The results showed that 37 persons (37%) were positive for H. pylori, and the remaining 63 (63%) were negative. The highest level of infections was among cases aged (32– 41) with a total of 15 infections (40.54%). It can be concluded that infection with Helicobacter pylori is prevalent in asymptomatic people of Zawia city.

Abstract 2199: Molecular dissection of a novel, highly proliferative lineage in a model of Helicobacter pylori-driven gastric metaplasia and dysplasia

Abstract At least 80% of gastric cancer cases are attributed to stomach infection with the bacterium Helicobacter pylori (Hp), which causes lifelong chronic inflammation. In some individuals, this inflammation can cause gastric atrophy, metaplasia (conversion of one normal cell type to another), dysplasia (presence of abnormal cells) and finally cancer, but the specific mechanism(s) through which Hp triggers this cascade are not well understood. Similar preneoplastic changes are recapitulated in a mouse model through tamoxifen-induced expression of active KRAS in the chief cells of the stomach (KRAS+ mice). We found that Hp infection of KRAS+ mice exacerbated disease: compared to Hp-KRAS+ mice, Hp+KRAS+ mice had an altered trajectory of metaplasia and accelerated dysplasia. In accordance with the hypothesis that Hp causes cancer through eliciting chronic inflammation, Hp+KRAS+ mice had severe inflammation marked by a ten-fold increase in T cells compared to Hp-KRAS+ mice. To understand gene expression changes in gastric cells in the context of metaplasia with and without active Hp infection, we performed single cell RNA-sequencing (scRNA-seq) on eight mouse stomachs (+/- Hp, +/- KRAS) using the 10x Chromium platform. Cluster analyses followed by UMAP (Uniform Manifold Approximation and Projection) visualization allowed us to identify 25 clusters, including epithelial and immune cell types. Hp+KRAS+ mice had a striking expansion of a population of metaplastic cells (most similar to surface mucus-producing “pit” cells), which comprised ~40% of epithelial cells in these mice. Metaplastic pit cells expressed markers of intestinal metaplasia and gastric cancer, and their abundance in the stomach was correlated with helper T cell abundance. Analysis of RNA velocity suggested that metaplastic pit cells arose from a progenitor cell type, rather than arising from pit cells directly. As well, a 10x Visium spatial gene expression experiment revealed the spatial organization of metaplastic pit cells near the lumen of the gastric epithelium. Finally, antibiotic eradication of Hp prevented metaplastic pit cell development and other disease phenotypes. Taken together, these studies provide new understanding of how Hp infection and inflammation cause gastric preneoplastic progression. Citation Format: Valerie Phoebe O'Brien, William C. Young, Greg Finak, Raphael Gottardo, Nina R. Salama. Molecular dissection of a novel, highly proliferative lineage in a model of Helicobacter pylori-driven gastric metaplasia and dysplasia [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 2199.

Abstract 226: Recurrent trisomies, variable selection and precancerous evolution in the normal gastric epithelium

Abstract Gastric cancer is the third leading cause of cancer-related mortality globally and is often associated with infectious agents such as the bacterium Helicobacter pylori. However, the background mutational landscape in normal gastric epithelium and the first genomic steps towards the formation of gastric cancer remain poorly understood. Here, we use whole-genome sequencing of microdissected gastric glands (n=271) from 30 patients, 18 of whom had gastric cancer. We show that gastric glands are clonal structures and accrue approximately 27 single base substitutions per year. While the mutational signatures in most normal glands reflect age-related mutagenesis, gastric glands sampled close to a tumor showed exposure to a mutagenic process enriched in tumors (COSMIC reference mutational signature SBS17). Phylogenetic analysis shows that acquisition of SBS17 substitutions is closely linked to overt malignant transformation. We also observe widespread trisomies of specific chromosomes, which are recurrently and independently acquired in many gastric glands of the same patient. Mutations in genes encoding epigenetic modifiers and chromatin remodelers showed evidence of positive selection and were highly enriched in some patients. This was confirmed by targeted sequencing of cancer genes in a further 1008 gastric glands. Strikingly, glands that exhibit driver mutations, a recurrent trisomy or elevated mutation loads only overlap minimally, suggesting a highly variable and patient-specific mutation and selection landscape in the normal gastric epithelium. Taken together, these results give novel insights into the preclinical evolution of gastric malignancies. Citation Format: Tim H. Coorens, Grace Collord, Hyungchul Jung, Yichen Wang, Sonia Zumalave, Daniel Leongamornlert, Luiza Moore, Krishnaa Mahbubani, Kourosh Saeb-Parsy, Suet Yi Leung, Michael R. Stratton. Recurrent trisomies, variable selection and precancerous evolution in the normal gastric epithelium [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 226.

Hubungan Antara Stress Dengan Kejadian Kekambuhan Gastritis Pada Remaja Di Pondok Pesantren X Kudus

AbstrakBackground: Gastritis is inflammation caused by infection or irritation of the gastric mucosa due to irregular eating patterns, consumption of coffee, alcohol, consumption of long-term pain relievers, psychological stress, physical stress, and infection by the Helicobacter pylori bacteria. Gastritis which causes stomach pain, such as bloating, nausea, and having a headache that causes uninterrupted daily activities, stomach cancer, and even death. Stress has a negative impact on the neuroendocrine digestive system, so you can be at risk of developing gastritis due to stressful conditions, and stomach acid will increase.Methods: the study was a cross sectional quantitative study. Data collection using a questionnaire with 120 respondents consisting of 60 cases 60 controls with purposive sampling technique. Data processing used the chi-square test.Results: The study was conducted on respondents who were homogeneous who were female and had the characteristics of the case group aged 16 years as much as 35 (30%), high school education as much as 32 (53.3%), and stress as much as 30 (50%). The results of the study in the case group showed that stress was 30 (90.9%) and no stress was 30 (9.1%).Conclusion: There is a relationship between stress and the incidence of gastritis recurrence in adolescents at Pondok Pesantren X Kudus, with the chi-square test the result is p value = 0,000. Key words: Adolescence, Stress and Gastritis Recurrence Incidence

Long-Term Impact of Helicobacter pylori Eradication Therapy on Gastric Cancer Incidence and Mortality in Healthy Infected Individuals: A Meta-Analysis Beyond 10 Years of Follow-Up

Gastric cancer remains the third commonest cause of cancer death worldwide,1 and the bacterium Helicobacter pylori is strongly implicated in its pathogenesis.2 In previous meta-analyses of randomized controlled trials (RCTs), we found that eradication of H pylori in healthy asymptomatic individuals reduces future incidence of, as well as mortality from, gastric cancer.3,4 In the most recent meta-analysis, including 7 RCTs that recruited 8323 participants, the number needed to treat (NNT) was 72 (95% CI, 56–199) to prevent 1 future gastric cancer and 135 (95% CI, 88–658) to prevent 1 death from gastric cancer.

Rational Drug Therapy of Peptic Ulcer Disease In Patients Admitted To Gastroenterology Ward of LRH Peshawar, Khyber Pakhtunkhwa, Pakistan

The present study was conducted from 12 Sep.2019 to 10 Nov. 2019 at the Gastroenterology ward, Lady Reading Hospital Peshawar Pakistan, aiming to accurately investigate the Peptic Ulcer Disease state, signs and symptoms, to ensure complete information about drug therapy and patients response. Peptic ulcer disease is defined as an upper gastric mucosal rupture due to acid hat results in the formation of ulcers that extend beyond the muscular mucosa to the submucosa. Peptic ulcer is characterized by abdominal pain, change in appetite, unexplained weight loss, nausea, vomiting, heart burn and dark, tarry stools. Spicy and acidic foods, Stress and restless lifestyle were considered to be the leading causes of peptic ulcer. However the Helicobacter pyloribacterium is responsible for up to 60% stomach ulcers. Frequent use of painkillers, old age, obesity Smoking, excessive alcohol use, too little sleep, type O blood and chronic stress, are the risk factors of peptic ulcers. Acute peptic ulcers results from taking aspirin or anti-inflammatory drugs. Chronic peptic ulcer represents a profound loss of substance. Data from a total of10 patients (6 female and 4 male)of all age, having various peptic ulcer diseases were involved in this study. It was concluded that most of the female patients (60%) were having peptic ulcers as compared to male patient (40%). It was found that 30-40 years old peptic ulcer patient percentage was 20%.Moreover it was observed that frequent use of Nonsteroidal Anti-inflammatory Drugs has been found to be the main causes of peptic ulcers

Cranberry: Chemical Composition, Antioxidant Activity and Impact on Human Health: Overview.

Cranberries are a rich source of bioactive compounds that comprise a healthy diet. Cranberry is abundant in nutritional components and many bioactive compounds that have antioxidant properties. Both American (Vaccinium macrocarpon) and European (Vaccinium oxycoccus) cranberry species are rich in polyphenols such as phenolic acids, anthocyanins and flavonoids, and is one of the few fruits that is high in proanthocyanidins, which is linked to many health benefits. The review systematizes information on the chemical composition of cranberry, its antioxidant effect, and the beneficial impact on human health and disease prevention after cranberry consumption, and in particular, its effect against urinary tract inflammation with both adults and children, cardiovascular, oncology diseases, type 2 diabetes, metabolic syndrome, obesity, tooth decay and periodontitis, Helicobacter pylori bacteria in the stomach and other diseases. Additional research needs to study cranberry proteomics profiling, polyphenols interaction and synergism with other biologically active compounds from natural ingredients and what is important in formulation of new functional foods and supplements.

RATIONAL DRUG THERAPY OF PEPTIC ULCER DISEASE IN PATIENTS ADMITTED TO GASTROENTEROLOGY WARD OF LRH PESHAWAR KHYBER PAKHTUNKHWA, PAKISTAN

The present study was conducted from 12 Sep.2019 to 10 Nov. 2019 at the Gastroenterology ward, Lady Reading Hospital Peshawar Pakistan, aiming to accurately investigate the Peptic Ulcer Disease state, signs and symptoms, to ensure complete information about drug therapy and patients response. Peptic ulcer disease is defined as an upper gastric mucosal rupture due to acid hat results in the formation of ulcers that extend beyond the muscular mucosa to the submucosa. Peptic ulcer is characterized by abdominal pain, change in appetite, unexplained weight loss, nausea, vomiting, heart burn and dark, tarry stools. Spicy and acidic foods, Stress and restless lifestyle were considered to be the leading causes of peptic ulcer. However the Helicobacter pyloribacterium is responsible for up to 60% stomach ulcers. Frequent use of painkillers, old age, obesity Smoking, excessive alcohol use, too little sleep, type O blood and chronic stress, are the risk factors of peptic ulcers. Acute peptic ulcers results from taking aspirin or anti-inflammatory drugs. Chronic peptic ulcer represents a profound loss of substance. Data from a total of10 patients (6 female and 4 male)of all age, having various peptic ulcer diseases were involved in this study. It was concluded that most of the female patients (60%) were having peptic ulcers as compared to male patient (40%). It was found that 30-40 years old peptic ulcer patient percentage was 20%.Moreover it was observed that frequent use of Nonsteroidal Anti-inflammatory Drugs has been found to be the main causes of peptic ulcers

Risk assessment of gastric cancer in the presence of Helicobacter pylori cagA and hopQII genes.

Helicobacter pylori bacterium is one of the most common bacterial infections globally and is the leading cause of indigestion, gastric and duodenal ulcers, and gastric cancer. This bacterium can escape the antibacterial effects of stomach acid by adapting to the inner layers of the stomach. It combines with the natural sugars in the gastric mucosa. The compound is so effective that it makes bacterium resistant. For genes related to the pathogenesis of H. pylori, using the existence of genes such as cagA, hopQI, and hopQII, PCR is performed on some of these genes to amplify fragments of different lengths. One of the less-studied cases is that two or more pathogenic genes are simultaneously associated with H. pylori. This study examined the frequency of diseases and healthy individuals infected with H. pylori and cagA and hopQII genotypes. To diagnose H. pylori infection in healthy and stomach cancer patients, the PCR products are electrophoresed on the agarose gel after glmM gene amplification by PCR. To this aim, stomach tissue biopsies were used for patients, and saliva was used for healthy individuals. For this purpose, 150 gastric biopsy samples from stomach cancer patients and 150 saliva samples from healthy people were collected. Data showed a significant relationship between the coexistence of two genes, cagA and hopQII, and stomach cancer. 34.2% of patients and 10.1% of healthy individuals showed two genotypes, while other healthy people (89.9%) infected with H. pylori did not have this genotype. Therefore, the simultaneous presence of these two bacterial genes in human societies can be an essential biomarker for the diagnosis and prognosis of gastric cancer.

Autophagy mediates ER stress and inflammation in Helicobacter pylori-related gastric cancer

ABSTRACT Autophagy is a cellular degradation mechanism, which is triggered by the bacterium Helicobacter pylori. A single nucleotide polymorphism (SNP) in the autophagy gene ATG16L1 (rs2241880, G-allele) has been shown to dysregulate autophagy and increase intestinal endoplasmic reticulum (ER) stress. Here, we investigate the role of this SNP in H. pylori-mediated gastric carcinogenesis and its molecular pathways. ATG16L1 rs2241880 was genotyped in subjects from different ethnic cohorts (Dutch and Australian) presenting with gastric (pre)malignant lesions of various severity. Expression of GRP78 (a marker for ER stress) was assessed in gastric tissues. The effect of ATG16L1 rs2241880 on H. pylori-mediated ER stress and pro-inflammatory cytokine induction was investigated in organoids and CRISPR/Cas9 modified cell lines. Development of gastric cancer was associated with the ATG16L1 rs2241880 G-allele. Intestinal metaplastic cells in gastric tissue of patients showed increased levels of ER-stress. In vitro models showed that H. pylori increases autophagy while reducing ER stress, which appeared partly mediated by the ATG16L1 rs2241880 genotype. H. pylori-induced IL-8 production was increased while TNF-α production was decreased, in cells homozygous for the G-allele. The ATG16L1 rs2241880 G-allele is associated with progression of gastric premalignant lesions and cancer. Modulation of H. pylori-induced ER stress pathways and pro-inflammatory mediators by ATG16L1 rs2441880 may underlie this increased risk.

Human microbiota. Friend? Enemy? Neighbors?

The review is dedicated to the modern ideas about the composition of the human intestinal microbiome, factors that determine the bacterial «landscape» and affect its activity. The main functions of normal microflora have been described, including intestinal motility, protection of intestinal barrier against pathogenic bacteria, parasites, intestinal epithelial regeneration, metabolic and immunological functions, participation in digestive processes, synthesis of amino acids and proteins, antibiotics, vitamins, hormonally active substances, promoting the absorption of minerals and nutrients, preventing the development of pathological conditions. Determination of intestinal microbiota expression of the innate immune system explains the role of microflora in chronic inflammation and diseases such as liver fibrosis, metabolic syndrome, type 2 diabetes mellitus, atherosclerosis, cardiovascular, neurodegeneration and cancer. A promising way is the use of microorganisms with beneficial properties (probiotics), the necessary substrate for them (prebiotics), their metabolites (metabiotics) and complexes of pro‑ and prebiotics (synbiotics) not only to restore and regulate the intestinal microflora, but also as therapeutic agents in some diseases, in particular during eradication therapy of the bacterium Helicobacter pylori. The functions of some species and strains of beneficial bacteria have being discussed, in particular the strain Bacillus causii UBBC‑07, as well as the results of preclinical and clinical trials of its use at antibiotic‑associated diarrhea in the inhibition of Clostridium difficile. The issues of safety and facts of positive impact on human health of probiotic products of Probeez line (Organosyn Ltd.) are considered, which differ in the personification of appointments to certain segments of the population: Probeez®, Probeez® Femina, Probeez® Kids, Probeez® DUO and Probeez® Immuno contain successfully selected live probiotic bacteria. The available range of monocomponent (Probeez® with Kids, Probeez® DUO), multicomponent (Probeez®, Probeez® Femina) and synbiotic (Probeez® Immuno) products allows to choose the best option in specific conditions for their application not only in inflammatory diseases of the gastrointestinal tract, but also in chronic disorders of other systems and organs.&#x0D;

In Silico Protein-Protein Interaction of Pterois volitans Venom with Cancer Inducers of Helicobacter pylori.

Gastric cancer is a pathological condition induced by the bacteria Helicobacter pylori. Targeting the key virulence factors of H. pylori causing gastric cancer is a promising method for treating gastric cancer. Recently, research has been focused on analyzing the adrenergic, cholinergic, and anti-cancer properties of their venom proteins. Testing the anti-cancer activity of the lethal proteins in the venom of P. volitans provides a bioactive compound for cancer treatment. Still, it is also helpful to eliminate the ecological imbalance caused by these fish in the marine environment. This study focuses on an in silico approach using Z-dock to analyze the bioactive prospective of the venom proteins of P. volitans against the essential virulence proteins of H. pylori responsible for inducing cancer. Our in silico docking study using a computational model of the venom proteins and H. pylori proteins has displayed the possible interactions between these proteins. The results revealed that P. volitans hyaluronidase and PV toxin's venom proteins effectively interact with H. pylori proteins Cag A, Cag L, GGT, Cag D, and urease that may be promising proteins in cancer therapy.