Iridoids and Triterpenoid Constituents of Hedyotis assimilis

Value of FINS Depends on Choice of DNA Locus as Shown in Baihuasheshecao

(2727) Proposal to conserve the name <i>Hedyotis caudatifolia</i> against <i>H. hui</i> (<i>Rubiaceae</i>)

Objective To establish a method for quality control of oleanolic acid and ursolic acid in Qingdu tablet. Methods The content of oleanolie acid and ursolic acid in Qingdu tablet were determined by high performance liquid chroma-tography(HPLC). The analytical column was kromasil- C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-0.5% phosphoric acid solution (88:12). The UV detection wavelenghth was 210 nm. The flow rate was 1.0 ml/min. Results Oleanolic acid and ursolic acid showed a good linearity in range of 0.06 - 0.54 μg (R=0.999 3) and 0.30 - 2.70 μg (R=0.999 7). The average recovery rates of Oleanolic acid and ursolic acid were 100.48% and 102.21%, respectively. Conclusions This method is simple, rapid and accurate. It could be used for the quality control and quality study of the Qingdu tablet. Key words: Qingdu tablet; Hedyotis diffusa; Oleanolic acid; Ursolic acid; High performance liquid chromatography; Quality control

Introduction: Hedyotis diffusa, known as Snake Needle Grass, is a herb used in traditional Chinese medicine for the treatment of multiple ailments, especially cancer. It is found to possess anti-proliferative, anti-inflammatory and anti-ageing properties.Aim: To study the free radical scavenging property of the ethanolic extract of Hedyotis diffusa using Nitric oxide assay.Materials and Methods: A dried sample of Hedyotis diffusa was extracted using 85% ethanol. Various concentrations of the extract were mixed with Sodium nitroprusside(SNP) in Phosphate Buffer Saline(PBS). Then Griess reagent was added and the absorbance studied using a spectrophotometer at 546nm. Quercetin solution was used as the standard.Result: The herb exhibited maximal activity of 72.28% at the concentration of 1000 ?g/ml. The IC50 value of Quercetin & Herb was found to be 10.24 g/ml & 104.18 g/ml respectively.Conclusion: The ethanolic extract of Hedyotis diffusa demonstrated a concentration-dependent free radical scavenging property which can be postulated as the mechanism of action in its anti-cancer, anti-inflammatory and anti-ageing properties.

ABSTRACT Hedyotis shiuyingiae is published as a new species from Hong Kong. The new species replaces what has been hitherto known as Hedyotis yangchunensis, a misapplied name.

Bouvardia rzedowskii, a new species from Oaxaca and Guerrero, Mexico, is described. We also note the rediscovery of the rare Oaxacan endemic Hedyotis xestosperma and transfer it to Bouvardia.

Triterpenoids and sterols from the stems of Hedyotis acutangula