Headspace Solid Phase Micro-extraction Combined With Gas Chromatography-mass Spectrometry Research Articles

Comparative Metabolomic Responses of Three Rhododendron Cultivars to the Azalea Lace Bug (Stephanitis pyrioides)

Rhododendron, with its high ornamental value and ecological benefits, is severely impacted by the azalea lace bug (Stephanitis pyrioides), one of its primary pests. This study utilized three Rhododendron cultivars, ‘Zihe’, ‘Yanzhimi’, and ‘Taile’, to conduct a non-targeted metabolomic analysis of leaf samples before and after azalea lace bug stress using headspace solid-phase microextraction combined with gas chromatography–mass spectrometry (HS-SPME/GCMS) and liquid chromatography–mass spectrometry (LCMS). A total of 81 volatile metabolites across 11 categories and 448 nonvolatile metabolites across 55 categories were detected. Significant differences in metabolic profiles were observed among the different cultivars after pest stress. A total of 47 volatile compounds and 49 nonvolatile metabolites were upregulated in the most susceptible cultivar ‘Zihe’, including terpenes, alcohols, nucleotides, amino acids, and carbohydrates, which are involved in energy production and secondary metabolism. Conversely, ‘Yanzhimi’ showed a downtrend in both the differential volatiles and metabolites related to purine metabolism and zeatin biosynthesis under pest stress. The resistant cultivar ‘Taile’ exhibited moderate changes, with 17 volatile compounds and 17 nonvolatile compounds being upregulated and enriched in the biosynthesis of amino acids, pentose, glucuronate interconversions, carbon metabolism, etc. The phenylalanine metabolic pathway played an important role in the pest resistance of different susceptible cultivars, and relevant metabolites such as phenylethyl alcohol, methyl salicylate, and apigenin may be involved in the plant’s resistance response. The results of this study provide a new perspective on the metabolomics of Rhododendron–insect interactions and offer references for the development of pest control strategies.

Microbial composition of spoiled irradiated ready-to-eat chicken feet and their spoilage characteristics

The spoilage of irradiated ready-to-eat chicken feet (RTECF) seriously affects the food's quality, resulting in package swelling and off-flavors, both of which are highly undesirable to stakeholders and consumers. To investigate the spoilage characteristics of irradiated RTECF and the microorganisms responsible for the spoilage and swelling, the changes in physicochemical properties, microbial community, and volatile organic compounds (VOCs) between normal and spoiled RTECF were evaluated. Compared with normal samples, the spoiled RTECF showed a higher pH value and total volatile basic nitrogen (TVB-N) value, lower color value, and texture features (P < 0.05). Acinetobacter, Pseudomonas, Lactobacillus, and Candida were the dominant genera responsible for RTECF spoilage as confirmed through both culture-dependent methods and high-throughput sequencing (HTS). The results of the verification for gas-producing strains showed that Lactobacillus brevis could cause RTECF packaging to swell. A total of 20 key VOCs were identified using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS). The results of Pearson correlation analysis (|r|>0.8, P < 0.05) showed that 12 dominant core microbial genera had a significant effect on the flavor of RTECF before and after spoilage. This study provides a theoretical reference for solving the problem of RTECF spoilage and improving the overall quality of RTECF products.

Effects of Wickerhamomyces anomalus Co-Fermented with Saccharomyces cerevisiae on Volatile Flavor Profiles during Steamed Bread Making Using Electronic Nose and HS-SPME-GC-MS.

Steamed bread is a traditional staple food in China, and it has gradually become loved by people all over the world because of its healthy production methods. With the improvement in people's living standards, the light flavor of steamed bread fermented by single yeast cannot meet people's needs. Multi-strain co-fermentation is a feasible way to improve the flavor of steamed bread. Here, the dynamic change profiles of volatile substances in steamed bread co-fermented by Saccharomyces cerevisiae SQJ20 and Wickerhamomyces anomalus GZJ2 were analyzed using the electronic nose (E-nose) and headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS). The five detectors of the E-nose rapidly detected the changes in volatile substances in different dough or steamed bread with the highest response value in co-fermented dough. A total of 236 volatile substances were detected in all the samples using HS-SPME-GC-MS, and alcohols were the most variable component, especially Phenylethyl alcohol. Significantly, more alcohols and esters were upregulated in co-fermented dough, and the addition of W. anomalus GZJ2 improved the key volatile aroma compounds of steamed bread using the relative odor activity value method (ROAV), especially the aldehydes and alcohols. Moreover, these key volatile aroma compounds can be quickly distinguished using the W2S detector of the E-nose, which can be used for the rapid detection of aroma components in steamed bread.

Study on VOCs of Fishmeal during Storage Based on HS-SPME-GC-MS.

Fishmeal is widely used in the feed industry as the main protein material. The freshness grade directly affects the quality of the fishmeal. During the storage of fishmeal, the odor would change accordingly as the freshness grades decreased. To study the characteristic volatile organic compounds (VOCs) of fishmeal, stored at 25 °C and 80%RH with different freshness grades, headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to analyze. The single-factor test was chosen for 50/30 μm divinylbenzene/carboxe/polydimethylsiloxane (DVB/CAR/PDMS) fiber. The equilibration time of 24 min, the extraction time of 60 min, the extraction temperature of 87 °C, and the addition of a saturated saline volume of 4 mL were determined by Box-Behnken design. There were 15 common VOCs detected during storage, the relative contents of acids increased significantly, ketones, aldehydes, esters, and nitrogen-containing compounds increased, and aromatic compounds and alcohols decreased. Combined with freshness indexes, volatile base nitrogen (VBN) and acid value (AV), hexadecanoic acid, tetradecanoic acid, methyl (Z)-N-hydroxybenzenecarboximidate, (Z)-hexadec-9-enoic acid, 6-ethoxy-2,2,4-trimethyl-3,4-dihydro-1H-quinoline, octadecanal, and [(Z)-octadec-9-enyl] acetate were determined as the characteristic VOCs based on the PLS-DA model. This study may provide data support for the development of fishmeal freshness-detecting instruments.

Development and validation of a high-throughput headspace solid-phase microextraction gas chromatography-mass spectrometry methodology for target and suspect determination of honey volatiles

The determination of volatile compounds is essential for the chemical characterisation of honey's aroma and its correlation to its sensory profile and botanical origin. The present study describes the development, optimization and validation of a new, simple and reliable method for the determination of volatile compounds in honey using headspace solid-phase microextraction combined with gas chromatography/mass spectrometry (HS-SPME-GC-MS). The optimization of the SPME conditions showed that the ratio of honey: water (2:1) and the incubation temperature (60 °C) are the most critical parameters. Gas chromatography was performed with medium polar Varian CP-Select 624 column and the experimental Retention Index for a number of compounds was determined as an additional identification feature for suspect analysis. The simultaneous use of four internal standards chlorobenzene, benzophenone, 2-pentanol and 4-methyl-2-pentanone and matrix matched calibration enhanced method accuracy achieving recoveries 73–114 % and repeatability ranging between 3.9 and 19 % relative standard deviations. Furthermore, the superiority of the HS-SPME to static head space technique was verified exhibiting four-to nine-fold higher sensitivity. Target and suspect screening were applied to 30 Greek honey samples and 53 volatile compounds belonging to different chemical classes, such as alkanes, aldehydes, ketones, alcohols, and esters were identified with quantified concentrations ranging between 3.1 μg kg−1 (Limonene) up to 20 mg kg−1 (Benzeneacetaldehyde). Among the new findings is the detection of Myrtenol in Greek pine honey and 2,3-butanediol in Greek oak honey. The developed analytical protocol can be a valuable tool in order to chemically characterize honey based on the volatile content.

Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 Isolated from the Seeds of Capsicum pubescens R & P Possess Antifungal Activity.

The Kosakonia cowanii Cp1 strain was isolated from seeds of Capsicum pubescens R. & P. cultivated in Michoacan, Mexico. Genetic and ecological role analyses were conducted for better characterization. The results show that genome has a length of 4.7 Mbp with 56.22% G + C and an IncF plasmid of 128 Kbp with 52.51% G + C. Furthermore, pathogenicity test revealed nonpathogenic traits confirmed by the absence of specific virulence-related genes. Interestingly, when fungal inhibitory essays were carried out, the bacterial synthesis of volatile organic compounds (VOCs) with antifungal activity showed that Sclerotinia sp. and Rhizoctonia solani were inhibited by 87.45% and 77.24%, respectively. Meanwhile, Sclerotium rolfsii, Alternaria alternata, and Colletotrichum gloeosporioides demonstrated a mean radial growth inhibition of 52.79%, 40.82%, and 55.40%, respectively. The lowest inhibition was by Fusarium oxysporum, with 10.64%. The VOCs' characterization by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) revealed 65 potential compounds. Some of the compounds identified with high relative abundance were ketones (22.47%), represented by 2-butanone, 3-hydroxy (13.52%), and alcohols (23.5%), represented by ethanol (5.56%) and 1-butanol-3-methyl (4.83%). Our findings revealed, for the first time, that K. cowanii Cp1 associated with C. pubescens seeds possesses potential traits indicating that it could serve as an effective biocontrol.

Tracing the Volatilomic Fingerprint of the Most Popular Italian Fortified Wines.

The aim of the current study was to provide a useful platform to identify characteristic molecular markers related to the authenticity of Italian fortified wines. For this purpose, the volatilomic fingerprint of the most popular Italian fortified wines was established using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS). Several volatile organic compounds (VOCs), belonging with distinct chemical groups, were identified, ten of which are common to all the analyzed fortified Italian wines. Terpenoids were the most abundant chemical group in Campari bitter wines due to limonene's high contribution to the total volatilomic fingerprint, whereas for Marsala wines, alcohols and esters were the most predominant chemical groups. The fortified Italian wines VOCs network demonstrated that the furanic compounds 2-furfural, ethyl furoate, and 5-methyl-2-furfural, constitute potential molecular markers of Marsala wines, while the terpenoids nerol, α-terpeniol, limonene, and menthone isomers, are characteristic of Vermouth wines. In addition, butanediol was detected only in Barolo wines, and β-phellandrene and β-myrcene only in Campari wines. The obtained data reveal an adequate tool to establish the authenticity and genuineness of Italian fortified wines, and at the same time constitute a valuable contribution to identify potential cases of fraud or adulteration to which they are subject, due to the high commercial value associated with these wines. In addition, they contribute to the deepening of scientific knowledge that supports its valorization and guarantee of quality and safety for consumers.

Differences in the Volatilomic Urinary Biosignature of Prostate Cancer Patients as a Feasibility Study for the Detection of Potential Biomarkers.

Prostate cancer (PCa) continues to be the second most common malignant tumour and the main cause of oncological death in men. Investigating endogenous volatile organic metabolites (VOMs) produced by various metabolic pathways is emerging as a novel, effective, and non-invasive source of information to establish the volatilomic biosignature of PCa. In this study, headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS) was used to establish the urine volatilomic profile of PCa and identify VOMs that can discriminate between the two investigated groups. This non-invasive approach was applied to oncological patients (PCa group, n = 26) and cancer-free individuals (control group, n = 30), retrieving a total of 147 VOMs from various chemical families. This included terpenes, norisoprenoid, sesquiterpenes, phenolic, sulphur and furanic compounds, ketones, alcohols, esters, aldehydes, carboxylic acid, benzene and naphthalene derivatives, hydrocarbons, and heterocyclic hydrocarbons. The data matrix was subjected to multivariate analysis, namely partial least-squares discriminant analysis (PLS-DA). Accordingly, this analysis showed that the group under study presented different volatomic profiles and suggested potential PCa biomarkers. Nevertheless, a larger cohort of samples is required to boost the predictability and accuracy of the statistical models developed.

Effects of Trellis Systems on the Vegetative Growth and Fruit Quality of Muscat-Flavored Table Grapes

The selection of appropriate grapevine trellis systems is of great importance for regulating vine vigor, forming grape yield, improving fruit quality, and labor-saving field management in the North China Plain. The effects of two trellis systems on the viticultural characteristics and fruit quality of three table grape cultivars: RuiduHongyu (RDHY), RuiduXiangyu (RDXY), and Red Globe (RG) were investigated. The two trellis systems were: (i) T trellis, with shoots positioned horizontally and downwards; and (ii) V trellis, with shoots positioned upright with an inclined angle. Headspace-solid-phase micro-extraction combined with gas chromatography mass spectrometry (HS-SPME-GC-MS) was used to determine the compositions and contents of the monoterpenes in the fruit. The results showed that for RDHY and RG, the T trellis showed better shoot growth consistency. The sugar–acid ratios of RDHY in 2019 and RDXY in 2021 under the T trellis were significantly higher than those under the V trellis. In 2020 and 2021, RDHY showed significantly higher total anthocyanin, flavonoid, and proanthocyanidin concentrations under the T trellis. The total monoterpene content in RDHY berries was significantly higher under the T trellis. The aromatic profiles of RDHY and RDXY grapes were similar and were mainly composed of citrus, other floral, other fruit, and rose aromatic characteristics. Among them, the main aromatic characteristics varied greatly among the different treatments. In conclusion, the Eurasian table grape cultivars with muscat flavor showed a more moderate and controllable vine vigor, consistent shoot growth, better fruit quality and taste, and greater accumulation of polyphenolic compounds and monoterpenes under the T trellis system.

Influence of Cold Pre-Fermentation Maceration on the Volatilomic Pattern and Aroma of White Wines.

Aroma compounds play a key role in wine quality due to their importance in wine aroma. The aim of the present study is to investigate the influence of cold pre-fermentative maceration (CPM) treatment on aromatic and sensory properties of white wines from four grape varieties (Tempranillo Blanco, Maturana Blanca, Viura and Garnacha Blanca) during two consecutive years (2019 and 2020). A total of 62 aroma compounds belonging to different chemical families were identified using headspace solid-phase microextraction combined with gas chromatography mass spectrometry (HS-SPME/GC-MS). CPM treatment enhanced the total relative concentration of alcohols, esters and acids compared to control wines. Regarding sensorial properties, esters made the greatest contribution to the studied white wines, mainly through the development of floral and fruity notes. On the other hand, CPM treatment did not significantly influence the total relative concentration of terpenoids, and different trends were observed according to grape variety and vintage. The obtained results showed differences in the wine's aromatic complexity according to the grape variety, the vintage and the treatment applied and suggested that CPM treatment could represent a suitable approach to manipulate the aromatic profile and enhance the aromatic quality and complexity of wine.

Key Aromatic Volatile Compounds from Roasted Cocoa Beans, Cocoa Liquor, and Chocolate

The characteristic aromas at each stage of chocolate processing change in quantity and quality depending on the cocoa variety, the chemical composition of the beans, the specific protein storage content, and the polysaccharides and polyphenols determining the type and quantity of the precursors formed during the fermentation and drying process, leading to the formation of specific chocolate aromas in the subsequent roasting and conching processes. Bean aroma is frequently profiled, identified, and semiquantified by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPMEGC-MS) and by gas chromatography olfactometry (GC-O). In general, the flavors generated in chocolate processing include fruity, floral, chocolate, woody, caramel, earthy, and undesirable notes. Each processing stage contributes to or depletes the aroma compounds that may be desirable or undesirable, as discussed in this report.

Analyzing Volatile Compounds of Young and Mature Docynia delavayi Fruit by HS-SPME-GC-MS and rOAV.

This study focused on the examination of the volatile compounds and fragrance properties of the young and mature fruit of Docynia delavayi. Headspace solid-phase microextraction combined with gas chromatography−mass spectrometry (HS-SPME-GC-MS) was applied for identifying 42 volatile compounds, with young and mature fruit containing 36 and 42 compounds, respectively. Heat map cluster analysis, principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), and independent sample t-testing were used to analyze sample differences. Based on a variable importance in projection (VIP) > 1 and p < 0.05, 23 key volatile compounds such as octanal, geranylacetone, butyl acetate, and dihydro-β-ionone were screened. β-Ionone and phenethyl acetate made the largest contribution to the aroma of D. delavayi after analyzing the relative odor activity value (rOAV) of the key volatile compounds and their aroma descriptors. Young D. delavayi fruit exhibited a prominent woody scent, while mature D. delavayi fruit had more intense floral and rosy aromas. The findings may lay a foundation for comprehensively developing and utilizing D. delavayi fruit.

Flavor and Functional Analysis of Lactobacillus plantarum Fermented Apricot Juice

The small white apricot is a juicy, delicious fruit with a short shelf life. Slight fermentation can significantly promote the flavors and nutrient value of apricot juice. This study used high-performance liquid chromatography (HPLC) and headspace solid-phase microextraction combined with gas chromatography–mass spectrometry (HS-SPME-GC-MS) to examine the physicochemical properties, nutritive value and flavor substances of apricot juice fermented by Lactobacillus plantarum LP56. Fermentation significantly increased lactic acid bacteria (LAB) and their product lactic acid, adding probiotic benefits to fermented apricot juice. In addition, the total phenolic compounds and antioxidant capacity increased, while the levels of soluble solids and organic acids decreased. Gallic acid, 3-caffeoylquinic acid and rutin mainly contributed to the antioxidant activity of fermented apricot juice. Alcohols, aldehyde, acid, ester, etc., were the main volatile compounds. Among the flavors, 12 substances with high odor activity values (OAV &gt; 1) were the key aroma-producing compounds with fruit, pine and citrus flavors. In conclusion, this study shows that L. plantarum LP56 fermentation can improve the nutritional value and aroma characteristics of apricot juice.

Removal of VOCs from wood by introducing activators to form hydroxyl radicals in-situ generation platform

Wood contains abundant extractives and volatile oils, resulting in the emission of hazardous volatile organic compounds (VOCs) and unpleasant odors. The homogeneous Fe2+ catalyzed Fenton reaction remains an attractive advanced oxidation process (AOPs) for the degradation of hazardous organic contaminants, but limited by due to the poor permeability of wood and wide distribution of extractives, the effective removal of wood extractives remained a challenge. Herein, a facile approach toward forming an in-situ ·OH radical generation platform was proposed by introducing EDTA-Fe2+ chelating agent into parenchyma cells and resin canals of wood by vacuum impregnation. Oxidation process was carried out using a “respiratory impregnation” method that imitated human respiration. The Electron paramagnetic resonance (EPR) analysis reveals that·OH was generated in-situ as the primary reactive species. Various controlling factors, including Fe2+ concentration, pH value, and H2O2 concentration were evaluated. The removal rate of VOCs was estimated by headspace solid-phase micro-extraction combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS). The best results (89.5% removal without cycling treatment) were observed at pH = 3, H2O2 = 20 mM and Fe2+ = 200 mM. This work improves the feasibility of using the Fenton system to remove VOCs from wood products.

Chemical constituents, antibacterial, acaricidal and anti-inflammatory activities of the essential oils from four Rhododendron species

As the ornamental plants and traditional medicines, Rhododendron przewalskii, R. anthopogonoides, R. thymifolium, and R. capitatum are widely distributed in western China. In this paper, the essential oils from these four species were extracted by supercritical extraction and the components were analyzed using headspace solid phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS), the antibacterial, acaricidal and anti-inflammatory activities were investigated. Results showed that R. thymifolium (RTEO) contained the highest yield of 0.99% with 246 compounds, followed by R. capitatum (RCEO, 0.81%) with 290 chemicals, R. anthopogonoides (RAEO, 0.57%) with 302 compounds and R. przewalskii (RPEO, 0.30%) with 294 components. They also exhibited the safety at given doses and have the anti-inflammatory in vitro and in vivo tests via inhibiting the cytokines productions, the acaricidal and antibacterial activities also were found. 4-Hydroxy-3-methylacetophenone from RPEO, α-pinene and β-pinene from RAEO, β-farnesene and germacrone from RTEO, and benzylacetone from RCEO, as main and active components, inhibited the NO content in RAW 264.7 cells induced by LPS. These results indicated that four essential oils have certain medicinal value and laid the foundation for the development of these species as raw materials for the pharmaceutical and perfume industries.

Effects of Fruit Bagging Treatment with Different Types of Bags on the Contents of Phenolics and Monoterpenes in Muscat-Flavored Table Grapes

The effects of fruit bagging treatments with seven different types of bags on the physicochemical characteristics of three table grape cultivars: RuiduZaohong (RDZH), RuiduHongyu (RDHY), and RuiduHongmei (RDHM) were investigated. Headspace-solid-phase micro-extraction combined with gas chromatography mass spectrometry (HS-SPME-GC-MS) was used to determine the compositions of monoterpenes in the fruit. The results showed that the total soluble solids in RDZH and RDHY fruits treated with the transparent, mesh, yellow, white, and blue bags were significantly higher than the control. The sugar–acid ratio of RDZH was optimized under the transparent bag and yellow bag treatments, and both significantly increased the sugar-acid ratio of RDHY and RDHM. Additionally, mesh bag, transparent bag, and white bag improved the contents of phenolics to a certain extent. The most abundant volatiles were linalool, geraniol, β-myrcene, β-cis-ocimene, and β-trans-ocimene, of which linalool was the main aroma component. The least squares discriminant analysis results showed that linalool, 4-terpineol, and terpinolen could be used to distinguish the main contribution of different bagging treatments for RDZH. Trans-isogeraniol, α-terpineol, and terpinolen could be used for RDHY. Trans-isogeraniol, β-myrcene, and terpinolen could be used for RDHM. In conclusion, transparent and white bags promoted the accumulation of phenolics and monoterpenes while pink and blue bags showed inhibitory effects.

Analysis of flavor-related compounds in fermented persimmon beverages stored at different temperatures

Storage temperature plays a very important role in the flavor development of fermented beverages. In this study, fermented persimmon beverage was stored for one year to study the changes in flavor-related compounds during storage at different temperatures. Fresh persimmon beverage and fermented beverages stored at 10, 20, and 30 °C had different tastes, distinguished by electronic tongue (ET). The fermented beverage was sweet when stored at 10 °C and salty when stored at 20 °C or above. A total of 658 nonvolatile metabolites were identified, among which quercetin and epicatechin were detected by a liquid chromatography-mass spectrometry (LC-MS) based metabolomics approach from four fermented beverage samples. The volatile compounds in the beverages were determined by headspace solid phase microextraction, combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and gas phase-ion migration spectroscopy (GC-IMS), respectively showing totals of 47 and 69 compounds in the four beverage samples. As detected by both methods, ethyl acetate, ethyl hexanoate, ethyl octanoate, ethanol, and acetic acid were all found in beverages stored at different temperatures and the results showed that a higher storage temperature resulted an increase in the number of volatile compounds in the beverage. However, fermented persimmon beverage stored at low temperatures had a better taste and flavor characteristics.

Correlation analysis of normal and moldy beef jerky microbiota with Volatile compounds

In this study, the microbial composition and volatile flavor components of normal beef jerky (NBJ) and moldy beef jerky (MBJ) from three different regions were examined using high-throughput sequencing (HTS) and headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC-MS) to explore the effects of microbial and environmental factors on MBJ, and the correlation between core microorganisms and volatile compounds were revealed using Spearman rank correlation analysis (|r | >1, P < 0.05). At the genera level, Aspergillus, Xeromyces and Wallemia were the dominant fungi. While Nicotiana_Otophora, Staphylococcus, Bacillus, Brochothrix and Acinetobacter were the dominant bacterial genera. 82 volatile compounds were identified in beef jerky from different regions. The correlation heat map between microorganisms and volatile compounds showed that eight core microbial genera had significant effects on the flavor quality of beef jerky after mold (VIP>1, P < 0.05), including five fungal and 3 bacteria, namely Arthrobacter, Xerochrysium, Dryomyces, Aspergillus, Colletotrichum, Bacillus, Staphylococcus and Salvia_pomifera. Bacillus, Staphylococcus, Salvia_pomifera and Colletotrichum had significant effects (P < 0.05) on 18 compounds, which may be classified as key microorganisms that affect beef jerky spoilage. Our study provides a theoretical reference for solving the problem of MBJ.

Effective degradation of VOCs from wood by Fe2+ chelate activated dual oxidant (H2O2-PS)

Wood is rich in extractives and volatile oils that emit unpleasant odors and some harmful volatile organic compounds (VOCs). Chemical oxidation technologies processes high efficiency on the destruction of aqueous organic components via oxidation by radicals, however, wood block treatment scenarios suffer from the low availability of radicals in aqueous conditions owing to the special structure of the wood blocks, limitations of mass transfer and the short life of free radicals. Herein, ethylenediaminetetraacetic acid (EDTA) is selected as a chelating agent to synthesize EDTA-Fe2+ chelate, thus introducing Fe2+ into the wood by vacuum impregnation. The Fe2+ is evenly distributed and immobilized in the wood to form a chemical oxidation system via in-situ activation of the dual oxidant (H2O2-PS), which truncates the contact distance between free radicals and extractives/volatile oils thus enhancing the removal efficiency. Various controlling factors, including EDTA/Fe2+ molar ratio, Fe2+dosage, PS/H2O2 molar ratio, and persulfate (PS) dosage are evaluated. The degradation products of VOCs by headspace solid-phase micro-extraction combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS) indicate that the wood VOC removal rate is ∼80%. The Electron paramagnetic resonance (EPR) analysis further reveals that SO4−· and ·OH are the primary reactive species. The characterization of wood properties illustrates that the process has no destructive effect. The results of this work may provide a theoretical basis for feasibility of the practical application of the EDTA-Fe2+/H2O2-PS system.

Characterization of the Volatile Profile of Cultivated and Wild-Type Italian Celery (Apium graveolens L.) Varieties by HS-SPME/GC-MS

Celery (Apium graveolens L.) is a vegetable belonging to the Apiaceae family that is widely used for its distinct flavor and contains a variety of bioactive metabolites with healthy properties. Some celery ecotypes cultivated in specific territories of Italy have recently attracted the attention of consumers and scientists because of their peculiar sensorial and nutritional properties. In this work, the volatile profiles of white celery “Sedano Bianco di Sperlonga” Protected Geographical Indication (PGI) ecotype, black celery “Sedano Nero di Torricella Peligna” and wild-type celery were investigated using head-space solid-phase microextraction combined with gas-chromatography/mass spectrometry (HS-SPME/GC-MS) and compared to that of the common ribbed celery. Exploratory multivariate statistical analyses were conducted using principal component analysis (PCA) on HS-SPME/GC-MS patterns, separately collected from celery leaves and petioles, to assess similarity/dissimilarity in the flavor composition of the investigated varieties. PCA revealed a clear differentiation of wild-type celery from the cultivated varieties. Among the cultivated varieties, black celery “Sedano Nero di Torricella Peligna” exhibited a significantly different composition in volatile profile in both leaves and petioles compared to the white celery and the prevalent commercial variety. The chemical components of aroma, potentially useful for the classification of celery according to the variety/origin, were identified.