Haze Development Research Articles

Thermodynamic Evolution of Plumes

Plumes are considered to play an important role in the origin of the solar wind. However, an understanding of their thermodynamic evolution is not complete. Here, we perform a detailed study of a plume inside a coronal hole throughout its lifetime, using the observations from the Atmospheric Imaging Assembly and the Helioseismic and Magnetic Imager. We find that the plume’s formation is preceded by frequent occurrences of small-scale jets and jetlets at its base, leading to the gradual development of plume haze. The plume rapidly developed within the first 6 hr into its well-known morphology. Light curves from all extreme ultraviolet channels exhibit a similar profile, suggesting its multithermal nature and intensity modulation over its lifespan. Moreover, the photospheric magnetic field dynamics at the plume’s base are highly correlated with its light curve in 171 Å. We calculate outflow velocities, observed prominently in the 171 Å passband and mildly in the 193 and 211 Å passbands, with median speeds lower in higher temperature bands but occasionally comparable to the respective sound speeds. When data are averaged over larger spatial scales, the plume appears isothermal along its length, with constant temperature throughout its lifetime. However, an analysis of the differential emission measure at full resolution reveals the presence of higher-temperature plasma, indicating internal temperature structures within the plume. These results provide new insights into the formation, dynamics, and thermal properties of coronal plumes, placing tighter constraints on models to understand their thermodynamic evolution and potential role in the solar wind.

Late Onset Corneal Haze Post Photorefractive Keratectomy

Introduction: Photorefractive keratectomy (PRK) is a surface ablation procedure to correct refractive errors. Regardless of the safety and efficacy, corneal haze may occur after PRK, and it remains one of the most feared complications because it can impair good visual outcomes. Case Presentation: A 20-year-old woman complained of blurred vision in her right eye (RE) after undergoing PRK elsewhere six months before admission with a history of refraction of S-12.75 C-1.75 x 10° preoperatively and S-0.75 postoperatively. Six-month postoperative uncorrected visual acuity (UCVA) was 0.01, best corrected visual acuity (BCVA) was 0.5 with S-7.00 C-0.75 x 50°. A slit lamp examination revealed grade three corneal haze. Anterior optical coherence tomography (OCT) showed the hyperreflective area with 132 μm deep into the stroma. The patient underwent phototherapeutic keratectomy (PTK) and mitomycin-C (MMC) treatment to a depth of 50 μm Ø6.5mm transition zone 0.5 mm. Two months later, UCVA was 0.2, BCVA was 0.63 with S-2.50 C-0.50 x 90°, and slit lamp examination revealed no haze remaining. Conclusions: This case illustrates the potential risk for corneal haze development, mainly when PRK is performed at greater treatment depths. However, with phototherapeutic keratectomy and mitomycin-C treatment, an excellent visual outcome and vision restoration can be obtained.

Stromal lenticule addition keratoplasty with corneal crosslinking for corneal ectasia secondary to FS-LASIK: a case series.

To explore the clinical efficacy and safety of stromal lenticule addition keratoplasty (SLAK) with corneal crosslinking (CXL) on patients with corneal ectasia secondary to femtosecond laser-assisted in situ keratomileusis (FS-LASIK). A series of 5 patients undertaking SLAK with CXL for the treatment of corneal ectasia secondary to FS-LASIK were followed for 4-9mo. The lenticules were collected from patients undertaking small incision lenticule extraction (SMILE) for the correction of myopia. Adding a stromal lenticule was aimed at improving the corneal thickness for the safe application of crosslinking and compensating for the thin cornea to improve its mechanical strength. All surgeries were conducted successfully with no significant complications. Their best corrected visual acuity (BCVA) ranged from 0.05 to 0.8-2 before surgery. The pre-operational total corneal thickness ranged from 345-404 µm and maximum keratometry (Kmax) ranged from 50.8 to 86.3. After the combination surgery, both the corneal keratometry (range 55.9 to 92.8) and total corneal thickness (range 413-482 µm) significantly increased. Four out of 5 patients had improvement of corneal biomechanical parameters (reflected by stiffness parameter A1 in Corvis ST). However, 3 patients showed decreased BCVA after surgery due to the development of irregular astigmatism and transient haze. Despite the onset of corneal edema right after SLAK, the corneal topography and thickness generally stabilized after 3mo. SLAK with CXL is a potentially beneficial and safe therapy for advanced corneal ectasia. Future work needs to address the poor predictability of corneal refractometry and compare the outcomes of different surgical modes.

Morphological Changes of Corneal Keratocytes following Surface Ablation Laser Surgery: An Observational Study from the United Kingdom

Introduction: Laser Subepithelial Keratomileusis (LASEK) is a type of surface ablation laser surgery that offers a solution to patients for correcting their ametropia. In rare instances, LASEK has been associated with complications such as the development of postoperative haze, infection, and poor visual outcomes. Imaging features detected within the patient’s cornea using In-Vivo Confocal Microscopy (IVCM) have aided in identifying the cellular basis of complications like these. Aim: To observe and describe the morphological changes seen on IVCM in patients following LASEK surgery. Materials and Methods: This observational study aimed to assess the quantity and morphology of anterior and posterior keratocytes in adults aged 18 years and older. Five participants (9 eyes) who presented for follow-up appointments following LASEK surgery at Manchester Royal Eye Hospital (MREH), United Kingdom, from July to December 2018 were recruited for the study. The cell counts of the anterior and posterior keratocytes were calculated using the Heidelberg Eye Explorer software. The images were analysed with the Image J program (National Institutes of Health, Bethesda, USA). Nerve fibers were assessed using the ACCMetrics program (University of Manchester, UK). The t-test was used to establish the statistical association between variables. Results: Morphological changes, such as zones of hyperreflectivity beneath the epithelium, were observed in all the recruited participants. Immune cells and sub-basal nerve abnormalities were detected in several participants. The number of keratocytes in the anterior stroma of all the participants was found to be much lower compared to that of the control. Only participant number 3 had a greater number of keratocytes in the anterior stromal layer (454 and 514 cells/mm2 ) compared to the other participants, who had a cell count ranging between 156 to 262 cells/mm2. Conclusion: It is evident that visible changes are noted both quantitatively and morphologically in both the anterior and posterior keratocytes postoperatively. More research is required with larger controlled studies to investigate the IVCM imaging biomarkers and morphological features that represent the wound healing process and the factors influencing visual outcomes, ensuring that postoperative complications can be minimised.

Characterization of fungal species involved in white haze disorder on apples in Northern Italy and description of Golubevia mali sp. nov. and Entyloma mali sp. nov.

White haze, caused by an extensive fungal colonization of the apple surface, is an emerging postharvest issue in several European apple production areas. It results in compromised quality and decreased marketability of the fruits, leading to economic losses. In this study, the occurrence and the diversity of white haze-related fungi associated with apples was investigated in Northern Italy. Fungal strains were isolated from apple tissues and the species diversity was assessed using molecular and phylogenetic tools. Moreover, the ability of the isolated species to reproduce symptoms on healthy apples artificially inoculated was tested. Seventy-two fungal isolates were collected. Six species belonging to different basidiomycetous genera (Entyloma, Golubevia, Tilletiopsis) were identified, showing high diversity of fungi involved in white haze development in Northern Italy. The strains were identified as E. belangeri, E. randwijkense, G. pallescens, T. washingtonensis. Moreover, two new species, E. mali sp. nov. and G. mali sp. nov. were described. The most frequently isolated strains were inoculated on healthy apple fruit, showing to be able to reproduce symptoms on red-skin apples, fulfilling Koch’s postulates. This work provides new insights to increase knowledge about the causal agents of white haze on apple. In addition, the names Golubevia, Golubeviaceae and Golubeviales have been nomenclaturally validated.

Management of Corneal Haze After Photorefractive Keratectomy.

Photorefractive keratectomy (PRK) is a safe and popular corneal surgery performed worldwide. Nevertheless, there is potential risk of corneal haze development after surgery. Proper management of post PRK haze is important for good visual outcome. We performed a comprehensive review of the literature on the various risk factors and treatments for PRK haze, searching the PubMed, Google Scholar, SCOPUS, ScienceDirect, and Embase databases using relevant search terms. All articles in English from August 1989 through April 2023 were reviewed for this study, among which 102 articles were chosen to be included in the study. Depending on the characteristics of and examination findings on post PRK haze, different management options may be preferred. In the proposed framework, management of PRK haze should include a full workup that includes patient's subjective complaints and loss of vision as well as visual acuity, biomicroscopy, anterior segment optical coherence tomography, epithelial mapping, and Scheimpflug densitometry. Topical steroid treatment for haze should be stratified based on early- or late-onset haze. Mechanical debridement or superficial phototherapeutic keratectomy (PTK) may be used to treat superficial corneal haze. Deep PTK and/or PRK can be used to treat deep corneal haze. Mitomycin-C and topical steroids are prophylactic post-surgery agents to prevent recurrence of haze.

Strong Haze‐Black Carbon‐Climate Connections Observed Across Northern and Eastern China

AbstractRadiative absorption of black carbon (BC) is of great importance in air quality and climate change. Simultaneous mitigation of haze pollution and climate warming requires an in‐depth understanding of haze‐BC‐climate connections based on direct observations. We simultaneously performed measurements in the North China Plain (NCP) and the Yangtze River Delta (YRD) during the winter of 2018–2019, when cross‐regional haze transport frequently occurred. We found that BC particles exhibited a thick coating thickness (100–120 nm) under haze pollution conditions in the NCP and were then transported to the YRD with regional haze development. BC particles in the downwind region showed a bimodal distribution of core‐size‐resolved aging degree, which can be used to separate and quantify local and regional BC. The results showed that the regional haze transport from the NCP brought 1–3 μg m−3 BC into the YRD. These regional BC particles exhibited ∼1.6 times stronger absorption efficiency than locally emitted BC. The direct radiative effects of BC in the downwind region were estimated to be enhanced by ∼2 times when regional haze pollution was transported from the NCP to the YRD. The amplification of BC's climate effect during regional haze pollution was attributed to the increases in both the mass concentrations and light absorption efficiency of BC, which were responsible for 40%–50% and 50%–60%, respectively. Our work identified strong haze‐BC‐climate connections across the NCP and YRD and highlighted the importance of BC in the effects of haze pollution on regional climate.

Development of interface haze after femtosecond laser-assisted in situ keratomileusis with accelerated corneal crosslinking: a case series.

Femtosecond laser-assisted in situ keratomileusis (FS-LASIK) with accelerated corneal crosslinking (FS-LASIK Xtra) is a recent procedure to achieve safer corneal ablation in myopic patients with borderline corneal thickness. Despite its well-accepted effectiveness, the development of remarkable interface haze is a potential concern but has rarely been reported and discussed. We report for the first time a case series of 11 eyes of 7 patients who developed typical interface haze 1-3months after FS-LASIK Xtra for the correction of myopia with astigmatism, with intensity grades ranging from 0.5 + to 3 + at the time of onset. The preclinical spherical diopters of the 7 patients ranged from - 2.25 D to - 9.25 D and cylindrical diopters ranged from - 0.25 D to - 2.50 D. The haze tended to be self-limiting, and topical anti-inflammatory therapy was given to moderate and severe cases, who responded well to treatment. The development of clinically significant interface haze is a relatively rare complication after FS-LASIK Xtra but tends to have a higher incidence and intensity compared to conventional stromal surgery such as FS-LASIK. Timely treatment and close follow-up are essential to patients undertaking FS-LASIK Xtra.

Changes in Secondary Inorganic Ions in PM2.5 at Different Pollution Stages Before and After COVID-19 Control

To investigate the change characteristics of secondary inorganic ions in PM2.5 at different pollution stages before and after COVID-19, the online monitoring of winter meteorological and atmospheric pollutant concentrations in Zhengzhou from December 15, 2019 to February 15, 2020 was conducted using a high-resolution (1 h) online instrument. This study analyzed the causes of the haze process of COVID-19, the diurnal variation characteristics of air pollutants, and the distribution characteristics of air pollutants at different stages of haze.The results showed that Zhengzhou was mainly controlled by the high-pressure ridge during the haze process, and the weather situation was stable, which was conducive to the accumulation of air pollutants. SNA was the main component of water-soluble ions, accounting for more than 90%. Home isolation measures during COVID-19 had different impacts on the distribution characteristics of air pollutants in different haze stages. After COVID-19, the concentration of PM2.5 in the clean, occurrence, and dissipation stages increased compared with that before COVID-19 but significantly decreased in the development stage. The home isolation policy significantly reduced the high value of PM2.5. The concentrations of NO2, SO2, NH3, and CO were the highest in the haze development stage, showing a trend of first increasing and then decreasing. The concentration of O3 was lowest in the pre-COVID-19 development stage but highest in the post-COVID-19 development stage. The linear correlation between[NH4+]/[SO42-] and[NO3-]/[SO42-] at different time periods before and after COVID-19 was strong, indicating that the home isolation policy of COVID-19 did not change the generation mode of NO3-, and the corresponding reaction was always the main generation mode of NO3-. The correlation between[excess-NH4+] and[NO3-] was high in different periods before COVID-19, and NO3- generation was related to the increase in NH3 or NH4+ in the process of PM2.5 pollution in Zhengzhou.

Tunning pectinase activity under the effects of electric fields in the enhanced clarification of wine must

IntroductionPectinolytic enzymes are of great importance for the clarification process of “Vinho Verde” wine must, contributing to the reduction of haze development. During the last decade, a growing body of knowledge has been established about the effects of electric fields on the activation of important food enzymes. However, the influence of electrical parameters on catalytic activity is enzyme-dependent and should be evaluated on a case-by-case basis. This work describes, for the first time, the effects of electric fields and electrical frequency on the activity of pectinase (PEC) in the accelerated clarification of “Vinho Verde” must.MethodModerate electric fields (MEF) with intensities below 20 V/cm and at electrical frequencies ranging from 50 to 20 kHz were applied at temperatures between 15 and 35°C. Enzymatic activity was measured for 25 min, and the initial rate of reaction was determined by the coefficient of the linear plot of galacturonic acid (GAL) production as a function of time.ResultsThe results show that electrical frequency can increase enzymatic activity depending on temperature conditions; at 20°C and with electrical frequencies of 2 and 20 kHz, enzymatic activity increased by up to 40 and 20%, respectively, when compared with the control sample (without the application of MEF). Temperature dependence was evaluated through the Arrhenius equation, showing that energy of activation (Ea) can be reduced from 9.2 to 6.6 kJ/mol at sub-optimal temperatures for PEC activity when MEF is applied.DiscussionElectrical parameters, when combined with temperatures below 20°C, reduced pectin concentration in “Vinho Verde” wine must by up to 42% of its initial content. This emergent treatment can be integrated in relevant environmental conditions, presenting an opportunity to increase enzyme efficiency even in low-temperature conditions, which favors the winemaking process.

Salivary flow and turbidity development inconsistently associated with lower taste intensity of vegetables and juices

The same phytochemicals that stimulate aversive sensations are often also responsible for purported health benefits in fruits and vegetables. Prior work indicates that some salivary proteins may reduce aversiveness of phytochemicals. In rodents, these salivary binding proteins have been shown to reduce bitter taste of polyphenols and alkaloids, but work in humans has focused primarily on polyphenol astringency (dry, rough, or puckery sensations). In this study, we tested if tastes of vegetable products might correlate to either salivary flow rate or the polyphenol binding capability of saliva, as measured by turbidity development when saliva is mixed with tannic acid. Participants (N = 26) provided chewing-stimulated saliva samples and rated five juices and two chopped vegetables for bitterness, sourness, and sweetness intensity. Saliva was mixed with tannic acid and light absorbance was measured for quantification of haze development. Greater absorbance corresponded to less bitterness for one green vegetable juice blend, less sweetness for two green vegetable juice blends and chopped kale, and less sourness from cranberry juice. Greater salivary flow corresponded to less bitterness from chopped brussels sprouts, and less sweetness from one green vegetable juice blend and chopped kale. These findings indicate that greater salivary flow rate and presence of certain salivary binding proteins is not universally associated with lower aversive tastes from phytochemical-containing foods. Whether associations between these salivary properties are ingredient specific or simply not robustly related to taste in commercial products should be further investigated.

Himawari-8 High Temporal Resolution AOD Products Recovery: Nested Bayesian Maximum Entropy Fusion Blending GEO With SSO Satellite Observations

High temporal resolution aerosol optical depth (AOD) observations derived from new-generation geostationary (GEO) satellite possess unique advantages in analyzing aerosol fast variation processes and thereby providing more accurate assessments on their climate effects and health risks. Unfortunately, the expected advantages and values are dramatically limited by relatively large proportion of data missing in the GEO AOD products due to cloud obscuration and intrinsic retrieval algorithm. Although several data recovery algorithms have been proposed in recent years to improve the spatial coverage for GEO AOD products, yet most of them aims at filling up the data blanks rather than reconstructing the temporally continuous variation of aerosol. Accordingly, in this study, a novel framework of nested spatiotemporal fusion blending GEO with sun-synchronous orbit (SSO) satellite observations based on Bayesian maximum entropy (BME) theorem is developed for GEO Advanced Himawari-8 Imager (AHI) AOD recovery with the sufficient excavation of complementary information from GEO and SSO satellite observations, where the minute-stage and hour-stage BME fusion are jointly employed to reconcile temporal inconsistency and data discrepancies between GEO and SSO observations. The results demonstrate that the AOD spatial coverage is dramatically increased by 240.9% (from 20.5% to 70%) with ensured accuracy after Nested-BME fusion. Additionally, two case analyses, during the development and dispersion processes of haze respectively, both demonstrate that the proposed Nested-BME fusion framework could reconstruct the reliable aerosol diurnal variation trends on the basis of recovering missing data for Himawari-8 AHI AOD datasets, while the AHI official level-2 and level-3 AOD products fail to capture these key trends. Furthermore, the developed Nested-BME AOD fusion framework is also applicable for other geostationary satellites over other regions, which could substantially enhance the availability and value of high temporal resolution AOD products for better scientific applications.

Characteristics of chemical composition and source apportionment of PM2.5 during a regional haze episode in the yangtze river delta, china

The source of PM2.5 varies at different stages of urban haze pollution. In addition, there is obvious regional transport of pollutants between urban agglomerations. PM2.5 and its major chemical compositions in a regional haze episode were measured continuously from 16 to 27 November 2018 in Nanjing, China. The types of primary sources resolved by principal component analysis (PCA) and positive matrix factorization (PMF) were similar, and the result of PMF was more refined. The average contribution of each source by PMF was: secondary nitrate (64.01%), secondary sulfate (11.62%), incomplete combustion (4.49%), sea salt (8.61%), biomass burning (6.90%), and crustal dust (4.37%). In different haze stages, the distribution characteristics of air pollutants differed. The concentrations of SO42-, NO3−, NH4+, and black carbon were the highest in the haze developing stage, which was 2.0, 3.1, 3.0, and 2.4 times, respectively, higher than that under clean conditions. The increment of NO3− dominated the development of haze, and the proportion of NO3− from haze generation to development increased by 4.05%. The concentration contributions of secondary nitrate, sea salt, and biomass burning were highest in haze development, secondary sulfate was highest in haze generation, and incomplete combustion was highest in haze dissipation, which was 3.5, 1.8, 3.3, 1.7, and 9.5 times higher than the clean stage, respectively. In the haze episode, the contribution of crustal dust was lower than in the clean stage. Potential source contribution function (PSCF) and concentration weighted trajectory (CWT) revealed that the major source area of air pollutants in Nanjing came from the southeast, and the northwest was the major impact area.

Evaluation of Coordinated Development of Logistics Development and Low-Carbon Economy in Wuhan Based on Big Data

Logistics industry in Wuhan city under the influence of economic development and related logistics policies, consumer demand is increasing, achieving a steady and rapid sustainable development. However, the ensuing high-energy consumption and high pollution have caused great pressure and negative impact on the environment of Wuhan city, and the pollution control problem has gradually aroused the common concern of the government, people, and relevant scholars, and the future development trend of low-carbon logistics has become a research hotspot of this new industry of logistics. In recent years, Wuhan city has been deeply affected by haze, and low-carbon and sustainable development is an important driving force for activities related to energy saving and emission reduction in Wuhan city at present. The logistics industry, as a high potential industry with high-speed development, is a key industry for environmental management and supervision, and the contradiction between the high-speed development of logistics industry and environmental pollution problem needs to be properly handled. This paper measures and analyzes the comprehensive efficiency of the logistics industry in Wuhan city from the perspective of low-carbon development, describes the current situation of the development of low-carbon logistics in Wuhan city and the problems that exist, analyzes them, and proposes feasible policies and establishments to make a contribution to the future development of low-carbon logistics in Wuhan city. This paper analyzes and organizes a large amount of literature, defines the definition of low-carbon logistics and performance evaluation, and selects the performance evaluation method that meets the research topic of this paper. On the basis of theoretical support, combined with the current situation of the development of low-carbon logistics industry in Wuhan city, reasonable indicators are selected for regression analysis of impact factors, and this paper’s low-carbon logistics efficiency evaluation index system is established to evaluate the performance of the low-carbon logistics industry in Wuhan city from 2010 to 2019, and finally put forward targeted policy recommendations.

Ammonium Chloride Associated Aerosol Liquid Water Enhances Haze in Delhi, India.

The interaction between water vapor and atmospheric aerosol leads to enhancement in aerosol water content, which facilitates haze development, but its concentrations, sources, and impacts remain largely unknown in polluted urban environments. Here, we show that the Indian capital, Delhi, which tops the list of polluted capital cities, also experiences the highest aerosol water yet reported worldwide. This high aerosol water promotes secondary formation of aerosols and worsens air pollution. We report that severe pollution events are commonly associated with high aerosol water which enhances light scattering and reduces visibility by 70%. Strong light scattering also suppresses the boundary layer height on winter mornings in Delhi, inhibiting dispersal of pollutants and further exacerbating morning pollution peaks. We provide evidence that ammonium chloride is the largest contributor to aerosol water in Delhi, making up 40% on average, and we highlight that regulation of chlorine-containing precursors should be considered in mitigation strategies.

Oxidation pathways and emission sources of atmospheric particulate nitrate in Seoul: based on <i>δ</i><sup>15</sup>N and Δ<sup>17</sup>O measurements

Abstract. PM2.5 haze pollution driven by secondary inorganic NO3- has been a great concern in East Asia. It is, therefore, imperative to identify its sources and oxidation processes, for which nitrogen and oxygen stable isotopes are powerful tracers. Here, we determined the δ15N (NO3-) and Δ17O (NO3-) of PM2.5 in Seoul during the summer of 2018 and the winter of 2018–2019 and estimated quantitatively the relative contribution of oxidation pathways for particulate NO3- and investigated major NOx emission sources. In the range of PM2.5 mass concentration from 7.5 µg m−3 (summer) to 139.0 µg m−3 (winter), the mean δ15N was −0.7 ‰ ± 3.3 ‰ and 3.8 ‰ ± 3.7 ‰, and the mean Δ17O was 23.2 ‰ ± 2.2 ‰ and 27.7 ‰ ± 2.2 ‰ in the summer and winter, respectively. While OH oxidation was the dominant pathway for NO3- during the summer (87 %), nighttime formation via N2O5 and NO3 was relatively more important (38 %) during the winter, when aerosol liquid water content (ALWC) and nitrogen oxidation ratio (NOR) were higher. Interestingly, the highest Δ17O was coupled with the lowest δ15N and highest NOR during the record-breaking winter PM2.5 episodes, revealing the critical role of photochemical oxidation process in severe winter haze development. For NOx sources, atmospheric δ15N (NOx) estimated from measured δ15N (NO3-) considering isotope fractionation effects indicates vehicle emissions as the most important emission source of NOx in Seoul. The contribution from biogenic soil and coal combustion was slightly increased in summer and winter, respectively. Our results built on a multiple-isotope approach provide the first explicit evidence for NO3- formation processes and major NOx emission sources in the Seoul megacity and suggest an effective mitigation measure to improve PM2.5 pollution.

Effect of mitomycin-C on corneal endothelial cell parameters after refractive surface ablation procedures.

The effect of mitomycin-C (MMC) on the reduction of endothelial cell count in the cornea remains controversial. We aimed to evaluate the effect of MMC on corneal endothelial cell parameters after refractive surface ablation procedures, including photorefractive keratectomy (PRK) and laser epithelial keratomileusis (LASEK). In this interventional, comparative, follow-up study, 342 eyes of 171 patients were followed up for 6 months. Patients undergoing PRK or LASEK were included and were divided into two groups: group one (188 eyes of 94 patients) with an ablation depth of ≥ 65 µm and who received intraoperative 0.02% MMC for 30 s, and group two (154 eyes of 77 patients) with an ablation depth of < 65 µm and who received balanced salt solution for 30 s. Changes in endothelial cell density (ECD), central corneal thickness (CCT), coefficient of variation (CV), and hexagonality values were compared between the groups at 3 and 6 months after surgery. The mean (standard deviaiton [SD]) age of the patients was 28.11 (6.56) years. The mean (SD) ECD did not change significantly in either group between the baseline and at 3 and 6 months postoperatively. The baseline mean ECD was significantly higher in group one than that in group two (P < 0.001) and remained so at 3 (P = 0.002) and 6 months (P = 0.022) postoperatively. The baseline hexagonality value was lower in group one (P = 0.173), with a gradual decrease during the postoperative follow-up as compared with that in group two (P = 0.016 and 0.001 at 3 and 6 months postoperatively, respectively). Group one had a significantly lower CCT at 3 and 6 months postoperatively (both P < 0.001) and a higher mean CV (3 months: P = 0.028; 6 months: P = 0.328). A single intraoperative application of MMC for 30 s as prophylaxis for corneal haze development during refractive surface ablation procedures had no significant effect on ECD up to 6 months postoperatively. Future studies with a contralateral-eye design (to neutralize factors specific to the individual patient), a larger sample size, and longer follow-up are necessary to confirm or disprove our observations.

Comparison of transepithelial and conventional photorefractive keratectomy in myopic and myopic astigmatism patients: a randomized contralateral trial

BackgroundTo assess transepithelial photorefractive keratectomy (tPRK) in terms of corneal epithelial healing rate, postoperative pain, postoperative discomfort, and visual and refraction outcomes compared to mechanical epithelial debridement PRK (mPRK) and alcohol-assisted PRK (aaPRK).MethodsIn this double-masked, randomized clinical trial, thirty-nine patients underwent tPRK in one eye and mPRK in the fellow eye (arm A), and 33 patients underwent tPRK in one eye and aaPRK in the contralateral eye (arm B). All surgical procedures were done using the Schwind Amaris excimer laser. The area of corneal epithelial defect in all eyes was captured and analyzed using ImageJ software.ResultsMean epithelial healing time was respectively 3.74 ± 0.82 and 3.59 ± 0.79 days in tPRK versus mPRK (P = 0.21) in arm A, and 3.67 ± 0.92 and 3.67 ± 0.74 days in tPRK versus aaPRK (P = 1.00) in arm B. Accounting for the initial corneal epithelial defect area, the epithelial healing rate was faster in conventional PRK groups compared to tPRK (both P<0.001) in both arms. However, there was no significant difference in safety, efficacy, spherical equivalent refractive accuracy, or corneal haze development between tPRK and conventional PRK groups (all P > 0.05).ConclusionsAll three methods are effective in terms of visual and refractive outcomes. However, although time to complete re-epithelialization was similar with the three methods, the epithelial healing rate was faster in conventional PRK considering the initial corneal epithelial defect area, and the patients experienced less pain and discomfort in the first postoperative day.Trial registrationIRCT, IRCT20200317046804N1. Retrospectively registered 5 May 2020.

Topical Pirfenidone-Loaded Liposomes Ophthalmic Formulation Reduces Haze Development after Corneal Alkali Burn in Mice.

Corneal chemical burns (CCBs) frequently result in corneal fibrosis or haze, an opacity of the cornea that obstructs vision and induces corneal blindness. Diverse strategies have been employed to prevent or reduce CCB-related corneal haze. In this study, we evaluated the physicochemical characteristics and biologic effects of a topical pirfenidone (PFD)-loaded liposomal formulation (PL) on a corneal alkali burn mice model. We found that PL was appropriate for ocular application due to its physiologic tear pH, osmolarity and viscosity suitable for topical ophthalmic use. Regarding its therapeutic activity, PL-treated mice had significantly reduced haze size and density, corneal edema, corneal thickness, and corneal inflammatory infiltration, in contrast to PFD in aqueous solution (p < 0.01). Importantly, the antifibrotic activity of PL (reduction of corneal haze) was associated with modulation of transforming growth factor (TGF)-β and Interleukin (IL)-1β genes. PL suppressed TGF-β expression and restored normal IL-1β expression in corneal tissue more efficiently in contrast to PFD in aqueous solution. In conclusion, PFD showed essential anti-inflammatory and anti-fibrotic effects in the treatment of alkali burns. Noteworthy, a new formulation of PFD-loaded liposomes remarkably improved these effects, standing out as a promising treatment for corneal haze.

Development of Haze Removing Hardware Using High-Level Synthesis

Development of Haze Removing Hardware Using High-Level Synthesis