This study examined the effect of prior biofeedback-assisted training of hand warming or hand cooling on the subsequent ability to train the opposite response. A two-group crossover design employed 43 subjects with one group receiving three days of hand warming followed by three days of hand cooling, while the second group received the opposite sequence. Analysis indicated subjects were able to produce the appropriate changes in hand temperature and were more successful at hand cooling than hand warming. During the transfer of training phase, subjects with prior training in hand cooling were able to produce warming above baseline levels compared with subjects initially trained to hand warm. In contrast, prior training of hand warming had no effect on the subsequent ability to cool hands.