I HAVE found from a good many years' experience that it is frequently difficult to assign any definite cause of death to the lower Vertebrata which die in the Zoological Society's Gardens from time to time. The examination of a large example of the Japanese salamander (Megalobatrachus japonicus), which lived for a good many (nineteen) years here, and measured some three feet in length, has suggested to me a rather curious and truly “natural” cause of death—if my inferences be correct. The animal showed no obvious signs of disease in any organ. Judging from its length it must have been old, for a specimen three feet, long is asserted to have been at least fifty-two years old (vide Gadow, Cambridge Natural History, “Amphibia and Reptiles,” p. 99), Comparing, this specimen with one some twenty inches in length I found that the size of the heart, as of the other organs, was, as might be expected, actually larger, but that all the subdivisions of the heart were of the same proportions in the two animals. But in the course of a dissection of the heart it was plain that the two series of valves, which lie respectively at the anterior and at the posterior end of the pylangium, were so small, relatively speaking, that, when forced backwards by the pressure of blood in the entire conus arteriosus, they would not meet in the middle line. On the other hand, in the smaller salamander the three Valves in question were in the first place situated closer together than in the large animal, being nearly in actual contact, and in the second place their size was so great in relation to the diameter of the pylangium that they would—or, I should father say, could—meet after the systole of the ventricle. The fact is that these valves do not appear to grow pari passu with the general increase in size of the heart and the conus arteriosus. My own observations as to the small size of the valves in the large example are quite in accord with those of Hyrtl (Cryptobranchus japonicus, Vindobonae, 1865), who dissected an animal two arid a half feet in length, and figures the valves, incorrectly as I believe in some particulars, but correctly in representing them to be of small relative size. It might be suggested, therefore, that the imperfection of the circulatory mechanism necessarily caused by the condition of the valves would lead to serious disturbances, and perhaps to death. If so the animal has a term put to its life by the mere fact that, while the heart grows with the increase in bodily size, the semilunar valves of the conus arteriosus do not.
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