The successful delivery of foreign material into plant tissues using gun powder driven microprojectile-mediated gene transfer system has been reported. In the gun powder driven microprojectile-mediated gene transfer system, the tungsten microprojectiles coated with the foreign genetic material is accelerated by an explosion of gun powder (Fig.1). The main disadvantages of the gun powder driven microprojectile are: variability of the velocity of the spent gas which can cause cell damage and random frequency of transformation, and the build up of unburned gun powder in the apparatus which can causeor create safety problems. An improved microprojectile for use in the genetic engineering ofplants which involves the use of air or gas pressure from a conventional pressurized air or gas supply as the accelerator has been developed. (Fig. 2). A comparison of the air driven and gun powder driven microprojectile is illustrated in electon micrographs of plant tissues bombarded by both methods (Figs. 5-8).
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