Guanyuan Acupoints Research Articles

Analysis of the Effect of Shenghua Zhuyu Herbal Acupoint Plaster in the Treatment of Postpartum Malodor

Objective: To assess the clinical effect of Shenghua Zhuyu ointment, which was applied onto the Zhongji, Qihai, and Guanyuan acupoints, on postpartum lochia. Methods: We randomly divided 166 patients who were admitted in 2018–2021 into an observation group and a control group. The control group was treated with conventional clinical methods, whereas the observation group was treated by applying Shenghua Zhuyu ointment on the Zhongji, Qihai, and Guanyuan acupoints. The amount and duration of lochia, abdominal pain, hospitalization time were observed; B-scan ultrasonography was performed to check whether the placenta was discharged. Results: The treatment received by the observation group of patients was shown to be more effective than that of the control group. Besides, the experimental group had less lochia than that of the control group. The observation group also had shorter duration of lochia and hospital stay than those of the control group (P < 0.05). The placenta excretion was better, and the degree of abdominal pain was lower in the observation group than those of the control group (P < 0.05). Conclusion: The acupoint application of Shenghua Zhuyu herbal acupoint plaster has a significant impact on retained placenta, in which it can aid the discharge of residual placenta and improve the patients’ physical functions and symptoms.

Effects of electroacupuncture at different acupoints on the histomorphology of neurogenic bladder and the expression of hyperpolarization-activated cyclic nucleotide-gated channels in interstitial cells of Cajal in a rat model of suprasacral spinal cord injury.

To investigate the effects of electroacupuncture at different acupoints on the histomorphology of neurogenic bladder and the expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in interstitial cells of Cajal (ICC) in a rat model of suprasacral spinal cord injury (SCI). A incomplete suprasacral SCI rat model was induced using a MASCIS impactor. Rats were randomly divided into a sham operation group, SCI model group, Ciliao treatment group or Guanyuan treatment group. The histomorphology of bladder cells was observed after hematoxylin and eosin (H&E) staining of bladder tissue sections. The expression of HCN channel proteins in ICC cells was detected by western blot and immunofluorescence, and HCN channel mRNA expression was measured using real-time PCR. In terms of histomorphology, the level of bladder cells after SCI increased significantly, and marked inflammation and edema were observed. Electroacupuncture treatment at the Ciliao acupoint significantly reduced inflammation and edema, whilst electroacupuncture treatment at the Guanyuan point partially reduced inflammation and edema. In terms of HCN channel protein and mRNA expression, western blotting, immunofluorescence and real-time PCR all confirmed that HCN channel expression after SCI was significantly upregulated, while electroacupuncture treatment at the Ciliao and Guanyuan acupoints inhibited HCN channel expression. Electroacupuncture treatment at the Ciliao acupoint significantly reduced histomorphological abnormalities in ICCs, and inhibited the expression of HCN channel proteins after SCI.

Repeated Electroacupuncture: An Effective Treatment for Hyperglycemia in a Rat Model

The effect of electroacupuncture (EA) in lowering blood glucose (BG) in eight fasted male obese Zucker diabetic rats aged 21–24 weeks was investigated. Group 1 (n = 4) received no EA and Group 2 (n = 4) received EA at the Zhongwan and Guanyuan acupoints on Day 1, Day 3, Day 6, Day 8, and Day 10. The BG level of the rats was measured at 10 minutes and 20 minutes after their nose had been inserted into the nose cone of an anesthetic apparatus; then, EA was applied for 30 minutes, and BG was measured again. At the end of the study, the animals were injected with sodium pentobarbitone (60 mg/mL, intraperitoneally), and blood and white adipose tissue were collected. On statistical analysis, Group 2 showed a significant decrease in mean BG at 20 minutes (baseline) and 50 minutes on Day 8 and Day 10 compared with Day 1 and Day 3, but such a decrease was not observed in Group 1. For Group 2, the baseline BG and the change in BG over 30 minutes were significantly lower on Day 8 and Day 10 compared with Day 1, Day 3, and Day 6, but not for Group 1. No significant differences were noted between the groups in serum insulin, glucose, insulin-to-glucose ratio, adiponectin, leptin, adiponectin-to-leptin ratio, and white adipose tissue.

Repeated Electroacupuncture in Obese Zucker Diabetic Fatty Rats: Adiponectin and Leptin in Serum and Adipose Tissue

Fasted, male, obese, Zucker, diabetic fatty rats aged 10–16 weeks were anesthetized with 1% halothane in nitrous oxide–oxygen (3:1) on alternate weekdays over 2 weeks. Group 1 (n = 4) did not receive electroacupuncture (controls); Group 2 (n = 4) received electroacupuncture using the Zhongwan and the Guanyuan acupoints; Group 3 (n = 4) received electroacupuncture using the bilateral Zusanli acupoints; Group 4 (n = 6) received neither halothane in nitrous oxide:oxygen nor electroacupuncture. At the end of study, animals were injected with sodium pentobarbitone (60 mg/mL, i.p.), and blood and white adipose tissue were collected. Analysis of variance and Duncan's tests showed that the mean leptin in serum was significantly lower and the adiponectin:leptin ratio was significantly higher in Group 2 than in Group 1 (p < 0.05); for Group 4, the serum leptin was significantly higher than it was for Groups 1–3 (p < 0.05), and the adiponectin:leptin ratio was significantly lower than it was for Group 2 (p < 0.05). Similar changes occurred for the leptin levels in the pelvic adipose tissue. In addition, for Group 2, the mean serum insulin: glucose ratio was significantly higher than it was for Group 1 (p < 0.05); for Group 4 the mean serum insulin and insulin: glucose ratio were significantly higher than they were for Groups 1 and 3 (p < 0.05), but not Group 2 (p > 0.05). No significant differences in the serum or the adipose-tissue measurements between Groups 1 and 3 were observed (p > 0.05).

Repeated Application of Electroacupuncture Ameliorates Hyperglycemia in Obese Zucker Diabetic Fatty Rats

Electroacupuncture (EA) was investigated for lowering the blood glucose (BG) in fasting male obese Zucker fatty diabetic (ZDF) rats aged 10–17 weeks. Anesthesia provided satisfactory chemical restraint to enable repeated EA. Animals in Groups 1, 2 and 3 were anesthetized on Days 1, 3, 5, 8, 10 and 12. Group 1 (n = 4) received no EA (controls), Group 2 (n = 4) EA at Zhongwan and Guanyuan acupoints, and Group 3 (n = 4) EA at both Zusanli acupoints. BG was measured at 10 and 20 minutes, and EA was applied for 30 minutes, after which BG was measured again. Group 2 had a significantly lower baseline BG at 20 minutes on Days 5, 8 and 12 and significantly less change in BG over 30 minutes on Days 3 and 5 than Group 1 (p < 0.05). Group 3 showed a significant decrease in the mean baseline BG compared to Group 1 in Week 1 (p < 0.05). Thus, repeated EA using Zhongwan and Guanyuan acupoints was effective in lowering the baseline BG and modulating the change in the BG in anesthetized animals.

Moxibustion Therapy at CV4 Prevents Postoperative Dysuria after Procedure for Prolapse and Hemorrhoids

Objective. To explore the intervention methods of the patients with dysuria after performing the procedure for prolapse and hemorrhoids (PPH). Methods. 100 cases with hemorrhoids were randomly divided into experimental and control groups. The control group received routine nursing care. As comparison, the experimental group, on the basis of conventional care, was treated with moxa roll moxibustion 1 hour after the operation for 30 minutes. The autonomous urination within 1 h, 2 h, 4 h, 6 h, and 8 h after operation and the catheterization rate 8 h after operation of two groups of patients were observed. Results. The median time of autonomous urination of control group (8 h) was significantly greater than that of the experimental group (6 h) (P < 0.001). Cox regression analysis showed that the moxibustion therapy was positively correlated with automatic micturition in the patients after PPH. The probability of automatic micturition in the experimental group was 2.032 times that in the control group (RR = 2.032, 95% CI: 1.278~3.230). The catheterization rate of control group (38%) was significantly higher than that of the experimental group (10%) (P < 0.001). Conclusion. The Guanyuan acupoint moxibustion can prevent dysuria after PPH and reduce the urethral catheterization.

Efectos antienvejecimiento de la moxibustión con cono de moxa como complemento de los antioxidantes que capturan radicales libres

Therapies that complement free radical scavenging are an important approach for treating aging in the brain. In the present study, two formulations of moxa cone moxibustion were applied at acupoints Zusanli (ST 36) and Xuanzhong (GB 39), and at acupoints Baihui (DU 20) and Guanyuan (RN 4), in D-galactose-induced senile mice. The results revealed that moxa cone moxibustion improved total superoxide dismutase and Cu/Zn-superoxide dismutase activity in the homogenates of the cerebral tissue, as well as ameliorating deficits in neuronal morphology and neuronal density in the cerebral cortex and hippocampal CA3. Moxa cone moxibustion also enhanced learning and memory functions of senile mice. Moxa cone moxibustion at Zusanli, Xuanzhong, Baihui and Guanyuan acupoints can thus be used to complement free radical scavengers, with efficacy that is equal to that of electroacupuncture at Zusanli and Xuanzhong, and superior to that of nimodipine treatment.

Effect of moxa at Guanyuan acupoint on QLQ of chemotherapy patients and Cx43 expression of the cyclophosphamide treated mice stomach

Effect of moxa at Guanyuan acupoint on QLQ of chemotherapy patients and Cx43 expression of the cyclophosphamide treated mice stomach

The effect of electro-acupuncture on bladder detrusor apoptosis in rats with urinary retention after spinal cord injury

Objective To study the effect of electro-acupuncture(EA)on bladder detrusor apoptosis in rats with urinary retention after spinal cord injury(SCI),compare the effectiveness of different acupoints and explore possible mechanisms.Methods One hundred female adult Sprague-Dawley rats were randomly divided into 5 groups:a normal group,a sham operation group,a model control group,a Guanyuan acupoint(RN4)EA group and a Shuidao acupoint(ST28)EA group,with 20 rats in each group.A model of urinary retention after SCI was established using the weight drop method.The EA groups were given EA once daily for 10 d.The other groups had no treatment.After 10 d of EA,an electron microscope was used to observe the bladder detrusor ultrastructure,and the terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) method and immunohistochemistry were used to observe any apoptosis in the detrusor smooth muscle cells and the expression of apoptosis-related genes bcl-2 and bax.Results After EA treatment,expansion of the rough endoplasmic reticulum was reduced significantly,and the number of edemic mitochondria was reduced compared with the model control group.In the normal and sham operation groups,little apoptosis was observed,and there was no significant difference between the two groups.Apoptosis indices(AIs)were higher in the model control group than in the normal group.The average AI in the Guanyuan EA group was lower than that in the Shuidao group.In the Guanyuan group,bax protein expression was less than that in the Shuidao group and the model group.In the Guanyuan group,bcl-2 protein expression was not significantly different from that in the Shuidao group and the model control group.Conclusion EA at the Guanyuan or Shuidao aeupoint can restrain bladder detrusor apoptosis in rats with urinary retention after SCI.EA may improve the ultrastructure of the detrosor urinae, reduce the expression of bax, change the ratio of bcl-2 to bax, and thus decrease apoptosis.The effect of EA at the Guanyuan acupoint is superior to that at the Shuidao acupoint. Key words: Electro-acupuncture; Effect of point; Guanyuan acupoint; Shuidao acupoint; Detrusor; Apoptosis