Bacteria isolated from suppressive composts which showed clear zones inhibitory to the growth of phytopathogenic fungi in vitro on agar plates were identified to be Bacillus subtilis. The suppressiveness of the culture broth of these bacteria became obvious in in vivo tests, and the efficacy of the broth to phytopathogens was equivalent to that of available chemicals. The suppressive spectrum of the broth of this bacteria was broad enough to be effective not only to phytopathogenic fungi but also to bacteria. As a suppressive substance, an antibiotic of the iturin group was purified from the broth and identified. MIC values of each component of the iturin to several phytopathogens were determined.
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