Growth Of Human Pathogenic Fungi Research Articles

The study of tryptophol containing emulgel on fungal reduction and skin irritation

Tryptophol (TOH), a fungal quorum-sensing molecule, that possesses anti-fungal activities for controlling the growth of human pathogenic fungi. In the present study, we developed TOH-containing emulgel formulations and examined the antifungal activities and potential use as topical treatments on the skin. The results showed that TOH-containing emulgel at 1000 μM has excellent physical characteristics as homogenous, stability, and inhibits the growth of 30 species of human pathogenic fungi in vitro. TOH-containing emulgel did not cause skin irritation in mouse model of irritation and in healthy human volunteers. Moreover, an increase in skin hydration and a decrease in trans-epidermal water loss (TEWL) were observed after TOH-containing emulgel treatment on human skin. Our findings indicated that TOH-containing emulgel can be utilize as an antifungal agent for topical treatment against fungal infections on the skin.

Evaluation of antifungal activity of different homoeopathic mother tinctures against Candida albicans

Objective: The aim of this study is to evaluate the antifungal activity of different homoeopathic mother tinctures against the growth of Candida albicans. Materials and Methods: Homoeopathic mother tinctures (ф) Syzygium jambolanum, Ficus religiosa, Ocimum sanctum, Allium cepa, Thuja occidentalis, Holarrhena antidysenterica and Eucalyptus globulus were evaluated for their antifungal activity against the growth of human pathogenic fungi C. albicans using paper disc agar diffusion method as per guidelines of clinical and laboratory standard (M44-A) with slight modification. The diameters of zone (mm) of inhibition were measured, and the obtained results were compared with that of the vehicle control. Ketoconazole was used as reference standard fungicide. Results: Seven homoeopathic mother tinctures were used in this study and the results indicates that some of the homoeopathic drugs in the study showed significant inhibitory activity against the growth of C. albicans as compared to control. Out of the seven medicines tested, S. jambolanum showed a maximum zone of inhibition as compared to other mother tinctures used. The effectiveness of zone inhibition against the growth of human pathogenic fungi C. albicans are S. jambolanum > T. occidentalis > A. cepa > F. religiosa > E. globulus > O. sanctum > H. antidysenterica. Conclusion: The present study suggests the inhibitory role of homoeopathic medicines against human pathogenic fungi C. albicans.

Rational design of N-alkyl derivatives of 2-amino-2-deoxy- d-glucitol-6P as antifungal agents

N-Alkyl and N, N-dialkyl derivatives of 2-amino-2-deoxy- d-glucitol-6P (ADGP) were synthesized and found to inhibit growth of human pathogenic fungi (MICs in the 0.08–0.625 mg mL −1 range for the most active compounds). It was thus shown that N-alkylation of ADGP provides novel inhibitors of a fungal enzyme, glucosamine-6P synthase, exhibiting higher antifungal activity than the parent compound, due to the increased lipophilicity and better uptake by fungal cells.