Grapevine Growth Research Articles

Anatomical and isotopic traits in grapevine wood rings record environmental variability

Summary In the Mediterranean region, prolonged droughts affect the growth and reproductive cycles of grapevine. Changes in the physiological processes of grapevine, consequent to variations in environmental factors or cultivation management, are recorded in wood anatomical and isotopic traits in grapevine stems. In this study, we measured the anatomical traits and stable carbon isotope content in the annual rings of Vitis vinifera L. subsp. vinifera ‘Falanghina’ in four vineyards located in southern Italy, characterised by different water availability. The aim was to investigate how wood anatomical traits respond to interannual climatic variations according to local conditions. Wood cores were taken from the stem of the grapevines and subjected to both microscopy and carbon stable isotope analyses to quantify functional wood anatomical traits, such as vessel size and frequency, and the intrinsic water-use efficiency of the grapevine. Wood traits were correlated with data on precipitation and temperature. The results showed that the plants at the four vineyards were characterised by differences in wood structure influencing the grapevine’s physiology under different conditions of water availability. Overall, the analyses showed that the grapevines at the wetter sites developed wood traits, e.g., wide vessels, which favour the efficiency of water flow, while at the drier sites, they developed plant traits, e.g., small vessels, which favour safety against embolism. However, the robustness of such main trends is trait-specific and is influenced by interannual climatic variability.

The Impact of High Temperatures in the Field on Leaf Tissue Structure in Different Grape Cultivars

Global warming will significantly affect grapevine growth and development. To analyze the effects of high temperature on the leaf tissue structure of grapevines in the field, 19 representative cultivars were selected from the grapevine germplasm resources garden in Turpan Research Institute of Agricultural Sciences, XAAS. Twelve tissue structure indexes of grapevine leaves, including the thickness of the upper epidermis (TUE), the thickness of palisade tissue (TPT), leaf vein (LV), the thickness of spongy tissue (TST), the thickness of the lower epidermis (TLE), stoma (St), guard cell (GC), cuticle (Cu), leaf tissue compactness (CTR) and leaf tissue porosity (SR), were measured during the natural high-temperature period in Turpan. The results showed significant differences in the leaf tissue structure of the 19 grapevine cultivars under natural high temperature. Based on the comprehensive comparative analysis of the leaf phenotype in the field, we identified that the leaves of some cultivars, including ‘Zaoxia Wuhe’, ‘Centennial Seedless’ and ‘Kyoho’ showed strong heat tolerance, whereas grapevine cultivars ‘Golden Finger’, ‘Shine Muscat’, ‘Flame Seedless’, ‘Bixiang Wuhe’ and ‘Thompson Seedless’ showed sensitivity to high temperature. We further evaluated the heat tolerance of different grapevine cultivars by principal component analysis and the optimal segmentation clustering of ordered samples. These findings provide a theoretical basis for adopting appropriate cultivation management measures to reduce the effect of high temperatures and offer fundamental knowledge for future breeding strategies for heat-tolerant grapevine varieties.

Organic nitrogen fertilization minimizes requirement of inorganic fertilizers and improves growth and yield attributes of superior grapevines

Purpose: Nitrogen (N) fertilization is the essential nutrient element that limits crop yields before any other element. However, excessive use of N fertilization often leads to economic loss and quality deterioration of the yield. Materials: In the present study, superior grapevines were fertilized with chemical N fertilizer, chicken manure, or biofertilizer (Nitrobeine) over three seasons (2018, 2019, and 2020) to quantify N usage from different sources. Results: The current study’s findings show that N in the mineral form at 60% to 80%, organic and biofertilizers at 20% to 40% was very effective in improving all growth characteristics and yield when compared to using nitrogen completely in mineral form or reducing the percentages of mineral to 40%. Reducing the ratios of mineral nitrogen from 100% to 40% and at the same time increasing the proportions of organic and biofertilizers from 0.0 to 60% caused a gradual increase in the improved berry quality, raising in TSS, % reducing sugars, TSS/acid ratio and decreasing in total % acidity in berries. Conclusions: To improve the yield of Superior grapevines quantitatively and qualitatively with minimum chemical N, it is advisable to supply the vines with the suitable nitrogen (72.0 g N/vine/season) as mineral N (ammonium nitrate) at 60%, organic (chicken manure) at 20% and bio-fertilizers (nitrobeine) at 20%. Thus, replacing 40% of mineral fertilizers with organic and bio-fertilizers can be recommended for producing organic berries.

Comparison of deep learning methods for grapevine growth stage recognition

Monitoring the phenological development stages of grapes represents a challenge in viticulture. It includes the phenological distinction of the growth stages of grapevines and the continuous technological developments, especially in computer vision, enabling a detailed classification of economically relevant development stages of grapes. In the present work, we show that based on a cascading computer vision approach, the development stages of grapes can be classified and distinguished at the micro level. In a comparative experiment (ResNet, DenseNet, InceptionV3), it could be shown that a ResNet architecture provides the best classification results with an average accuracy of 88.1%.

Effect of PGR on Growth and Establishment of Grapes (Vitis vinifera L.) cv. Thompson Seedless under Prayagraj Agro Climatic Condition

Grapes (Vitis vinifera) is one of the important commercial subtropical crops, which is a good source of vitamins and minerals. It is grown throughout the world except the places with extreme temperature and high altitude .So, to find out Effect of PGR on growth and establishment of grapes cv. Thompson Seedless under Prayagraj agro climatic condition; a field experiment was conducted at Horticulture Research Farm, Department of Horticulture, Naini Agriculture Institute, Sam higginbottom University of Agriculture, Technology and Science, Prayagraj, U.P-211007 during the year 2022. The experiment comprised of 10 different treatment of three different plant growth regulator comprising of GA3, IAA and IBA with different concentration of 50 ppm, 100 ppm and 150 ppm; 100 ppm, 300 ppm and 500 ppm; 1000 ppm, 2000 ppm and 3000 ppm respectively. The main objective of the experiment was to evaluate the root and vegetative growth of grapevines with their success and survival in the Prayagraj agro-climatic zone. From the present investigation, treatment T9 (IBA @3000 ppm) was found best with least days to emerge from the bud from the grapes cutting (6.4 days) with maximum amount of sprouting percentage (77.7%). It also showed the best root growth parameters with maximum root length 25.1cm @120DAP and maximum number of roots per cutting 49.3 @120DAP. It also showed best vegetative growth parameters with maximum shoot length 13.4cm @120DAP, maximum stem diameter 28.1mm @120DAP, highest number of leaves with 12.5 @120 DAP, maximum leaf area 73.9cm2, maximum plant height after transplanting 14.9cm and highest survival percentage 88.8% of grapes plant. The T9 showed the best results with all major parameters when cuttings were treated with different concentrations of plant growth regulators.

Physiological and Nutritional Responses of Ungrafted Merlot and Cabernet Sauvignon Vines or Grafted to 101-14 Mgt and 1103P Rootstocks Exposed to an Excess of Boron

The aim of this study is to analyze the effect of excess boron (B) on the nutrient uptake, growth, and physiological performance of grapevines. Merlot and Cabernet Franc grapevines, either own-rooted or grafted onto 1103P and 101-14 Mgt rootstocks, were exposed in a hydroponic pot experiment to 0.5 mM boron for sixty days. Twenty-five days following the beginning of B treatment, the first symptoms of boron toxicity appeared, including leaf edge and margin yellowing, subsequent necrosis, and cupping of leaf blades. At harvest, sixty days after the start of the experiment, B concentration of the treated vines increased in all parts of the vine in the following order: Leaves > Roots > Trunks > Shoots. Leaf Boron concentration in treated vines ranged from 980.67 to 1064.37 mg kg−1 d.w. Boron excess significantly reduced the concentrations of all macro and micronutrients studied in this experiment. The total leaf chlorophyll (Chl) concentration decreased from 35.46 to 44.45%, thirty and sixty days, respectively, from the beginning of the boron treatments. In addition, an excess amount of boron resulted in a dramatic decrease in net CO2 assimilation rate, stem water potential, and PSII maximum quantum yield, irrespective of vine type (own-rooted or grafted). At the end of the experimental period, the total leaf phenolic content increased by 71.73% in Merlot and by 71.16% in Cabernet Franc due to Boron stress. The tressed vines grafted onto 1103P showed increased shoot and root dry weights, leaf chlorophyll content, CO2 assimilation rates, and Fv/Fm ratio compared to vines grafted onto 101-14 Mgt. It was found that rootstocks play an important role in B toxicity. The results showed that the leaf accumulation of boron was delayed to a greater extent by 1103P rootstock compared to 101-14 Mgt, resulting in the earlier appearance of leaf toxicity symptoms in vines grafted onto 101-14 Mgt rootstock compared to 1103P.

Resistant rootstocks mitigate ionic toxicity with beneficial effects for growth and photosynthesis in grapevine grafted plants under salinity

The identification of salt-tolerant rootstocks can be an important strategy for producing vines in salt-affected areas. In this study, was tested the hypothesis that resistant rootstocks can mitigate the salinity damages by the lower ionic toxicity associated to best growth and photosynthesis in grapevine grafted plants. The four grapevine (Vitis spp.) rootstocks (‘IAC 313,’ ‘IAC 572,’ ‘SO4’ and ‘1103P’) and the grafted plants of ‘BRS Vitória’ variety on these rootstocks were exposed to increase NaCl (0, 40 and 80 mM NaCl) for the salt treatments. Under salinity, ‘IAC 313′ and ‘IAC 572′ rootstocks showed lower Na+content and higher K+ levels in leaves relative to other rootstocks. This response was associated with a higher leaf K+/Na+ ratio in the grafted and nongrafted plants of the ‘IAC 313′ and ‘IAC 572′ rootstocks. Based on the root and leaf dry weight (DW), the growth of the ‘IAC 313′ rootstock was unaffected by salinity, while ‘IAC 572′ showed a lower loss of DW relative to 1103P and SO4 rootstocks. All four rootstocks experienced increased membrane damage under saline conditions, but lipid peroxidation was lower in the ‘IAC 313′ rootstock. Grape plants grafted with the ‘BRS Vitória’ variety onto the IAC rootstocks (Vit/IAC 313 and Vit/IAC 572) presented higher efficiency of gas exchange and electron flow under salt stress, traits that were influenced by the rootstock genotype. Photochemical quenching (qP) and the electron transport rate (ETR) decreased under saline conditions, a response that was more intensive in Vit/1103P plants. In summary, our data showed that among evaluated rootstocks the ‘IAC 313′ and ‘IAC 572′ are better acclimated to salt and can be considered for vine production in salt-affected areas.

Viticultural Manipulation and New Technologies to Address Environmental Challenges Caused by Climate Change

Climate change is a critical challenge for the global grape and wine industry, as it can disrupt grapevine growth, production, and wine quality. Climate change could influence the cost-effectiveness and growth of the wine industry in different wine regions since grapevine development is deeply dependent on weather (short-term) and climate (long-term) conditions. Innovation and new technologies are needed to meet the challenge. This review article addresses the impact of climate change on grapevines, such as vine phenology, pest and disease pressure, crop load, and grape and wine composition. It also reviews recent advances in the areas of viticultural manipulation and relevant technologies to potentially reduce the impact of climate change and help growers improve grape quality. Remote sensing is used for vineyard microclimate monitoring; thermal sensors combined with UAVs, aircraft, or satellites are used for water management; soil electrical conductivity sensors have been developed for soil mapping. Viticultural manipulations, such as regulated deficit irrigation for water use efficiency and berry-ripening delay for growing quality fruit, are also discussed. The review assesses future directions for further technological development, such as soil and vine water monitoring devises, precision viticulture, and artificial intelligence in vineyards.

Influence of Cane Length and Number on Bud Behavior, Growth and Productivity of Black Magic Grapevines

Influence of Cane Length and Number on Bud Behavior, Growth and Productivity of Black Magic Grapevines

Propagation of Grape by Nodal Stem cuttings

Experiments were conducted to test the effect of MS saltsconcentration, potassium dihydrogen phosphate and growth regulators onshoot proliferation and growth in grapevine (Vitis vinifera L.), usingexplants derived from in vitro grown plantlets. Murashige and Skoogoriginal medium was used as basal medium. Shoot proliferation occurredprincipally when using nodal stem segments as explants. Among MS-saltstrengths tested, full MS- strength (1X) was optimum for shootproliferation and elongation. Increasing the original phosphorusconcentration in the medium by the addition of 170 mg/L KH2 PO 4 had apositive, marked effect on shoot number and shoot length. The nodal stemsegment explants responded best to relatively low concentrations of NAAand kinetin, and a large number of shoots and good plantlets height wereobtained on the medium containing 0.1mg/L NAA and 1.0 mg/L kinetin.Root formation occurred on the medium containing 0.3 mg/L IBA.

EFFECT OF SPRAYING POTA CRASTAL COMPOUND AND VAST TRIVALIOUM COMPOUND ON GROWTH AND NUTRITIOANL STATUS OF SUPERIOR GRAPEVINES

This study was carried out in three seasons 2019, 2020 and 2021 to examine the effect of spraying Pota crastal or Vast trivolium compounds each at 0.1% either once, twice or thrice on growth and vine nutritional status of Superior grapevines grown under El- Minia region.

Effect of Bio-stimulant Application on Growth, Yield and Quality of Thompson Seedless

Bio-stimulants offers a novel approach to enhance crop growth, quality and ultimately final yield of the produce. Nowadays, bio-stimulants containing Ascophyllum nodosum seaweed extracts and protein hydrolysates are increasing interest of scientists and farming communities. However, the potential of many of these bio stimulants products has not been fully exploited in grapes due to the lack of available scientific data. Grape growing under tropical conditions of India faces various type abiotic and biotic stresses. To support grapevine growth as well as yield and quality, bio-stimulant application can play an important role. Present experimentation was conducted at the farm of ICAR-National Research Centre for Grapes, Pune during the year 2020-21 with the objective to assess the effects of bio-stimulant application on growth, yield and quality of Thompson Seedless grapes. Different doses of bio-stimulant were applied on vines at different crop growth stages such as 3-4 leaf stage, pre-bloom stage and after berry setting, followed by two bunch dipping with varied concentrations. Among the different treatments T7 [RDF + 1.5 % (Spray: 3rd) + 0.2 % Bunch dipping (Stage 1) + 0.5 % Bunch dipping (stage 2)] found better in terms of bunch and berry quality parameters. Obtained results suggested that the application of bio-stimulant (Grade-I) affects vine growth, yield and quality of the Thompson Seedless grape in positive manner.

LiDAR Is Effective in Characterizing Vine Growth and Detecting Associated Genetic Loci.

The strong societal demand to reduce pesticide use and adaptation to climate change challenges the capacities of phenotyping new varieties in the vineyard. High-throughput phenotyping is a way to obtain meaningful and reliable information on hundreds of genotypes in a limited period. We evaluated traits related to growth in 209 genotypes from an interspecific grapevine biparental cross, between IJ119, a local genitor, and Divona, both in summer and in winter, using several methods: fresh pruning wood weight, exposed leaf area calculated from digital images, leaf chlorophyll concentration, and LiDAR-derived apparent volumes. Using high-density genetic information obtained by the genotyping by sequencing technology (GBS), we detected 6 regions of the grapevine genome [quantitative trait loci (QTL)] associated with the variations of the traits in the progeny. The detection of statistically significant QTLs, as well as correlations (R2) with traditional methods above 0.46, shows that LiDAR technology is effective in characterizing the growth features of the grapevine. Heritabilities calculated with LiDAR-derived total canopy and pruning wood volumes were high, above 0.66, and stable between growing seasons. These variables provided genetic models explaining up to 47% of the phenotypic variance, which were better than models obtained with the exposed leaf area estimated from images and the destructive pruning weight measurements. Our results highlight the relevance of LiDAR-derived traits for characterizing genetically induced differences in grapevine growth and open new perspectives for high-throughput phenotyping of grapevines in the vineyard.

EFFECT OF EARLY BERRY THINNING AND GIRDLING ON GRAPE QUALITY OF CV. VICTORIA

The research was carried out at University of Belgrade Faculty of Agriculture experimental station “Radmilovac” on table grape Victoria cv with aim to improve grape quality and with consideration the introduction of these ampelographic measures in regular grapevine growing. The research included two treatments of berry thinning (thinning bunch tip - BtT and first lateral wink -BtW), girdling (G) and control (C). Combined effect of berry thinning and girdling were not examined. The following features were examined: fertility and yield, bunch and berry dimensions, bunch and berry structure, sugar content and total acidity. The thinning treatments did not have significant effect on uvometric parameters and grape quality, but they improved the yield by 41.7% (BtW) and 51.4% (BtT) in comparison to the control. It can be explained by bad fruit set influenced by unfavourable meteorological conditions during flowering in control treatment. Berry thinning achieves a better fruit set. Berry thinning should be applied as a regular ampelographic measure according to weather conditions during flowering phonological stage. Results confirm the positive influence of girdling. The yield was increased by 88,34% compared to control, berries had a higher diameter (for about 1mm with respect to control), fruit set and bunch mass were in higher level against other treatments, sugar content (17,00%), total acidity (5,75 g/L) were improved too. Girdling can be introduced as a regular ampelographic measure in the table cultivars vineyards, in order to obtain higher yields and better quality of table grape.

The Biocontrol Potential of Endophytic Trichoderma Fungi Isolated from Hungarian Grapevines, Part II, Grapevine Stimulation.

In the first part of this two-piece publication, the isolation, identification and in vitro characterization of ten endophytic Trichoderma isolates were reported. Here we report the ability of two different mixes of some of these isolates (Trichoderma simmonsii, Trichoderma orientale and Trichoderma gamsii as well as of Trichoderma afroharzianum and T. simmonsii) to colonize and stimulate the growth of grapevines. Two commercial vineyards about 400 km away from the site of isolation were used as experimental fields, from which the strains of three Trichoderma species were re-isolated up to four years after rootstock soaking treatment with conidiospores, performed before planting. The treatments decreased the overall percentage of lost plants of about 30%, although a low number of lost plants (about 5%) were observed also in the control plot. For all cultivars and clones, the Trichoderma treatments significantly increased both the bud burst ratio and bud burst vigor index. In addition, the grape must parameters such as the Brix degrees, as well as the extract, the D-glucose and the D-fructose concentrations all appeared to be improved, suggesting a potentially higher ethanol content of the produced wine. We conclude that grapevine-endophytic Trichoderma isolates promote plant growth, which could be a useful feature for sustainable agriculture in general and integrated plant production in particular.

In grapevine decline, microbiomes are affected differently in symptomatic and asymptomatic soils

Grapevine decline affects viticulture worldwide. It is caused by a wide range of individual and combined biotic and abiotic factors. Some declines remain unexplained because they are not associated with known pathological symptoms or mineral dysregulation. Vineyard microbiological quality is an important area of study, since grapevine-associated microbiome primarily originates in vineyard soils and determines host health and development. To understand the decrease of growth and yield, and the high mortality of plants in vineyards affected by these declines, a multisite study investigated soil microbial communities. Spatial (terroir: two distinct geographical locations) and temporal (season: autumn and spring) dimensions were added to the inter-row soil status factor (S for areas with symptomatic vines and AS for those with asymptomatic vines). The microbiomes of AS and S soils were analyzed using high-throughput sequencing based on the bacterial 16S rRNA gene, the fungal ITS1 region (Internal Transcribed Spacer), or the fungal 18S rRNA gene for Glomeromycota family. Geographical location was the strongest driver of bacterial and fungal microbial communities, while the seasonal factor primarily influenced bacterial community. Based on metabarcoding analysis, symptomatic soils presented enriched bacterial taxa that can be potentially beneficial for grapevine growth. In addition, fungal diversity and richness, including Glomeromycota division, were greater in symptomatic soils. Fungal genera associated with grapevine diseases were detected across all conditions, with higher abundances in symptomatic soils. These findings reveal that vineyard soils affected by unexplained decline are a potential source of both fungal pathogens and beneficial microorganisms.

Spatial Analysis of Aridity during Grapevine Growth Stages in Extremadura (Southwest Spain)

Aridity is a key determinant of agriculture worldwide due to rising temperatures, rainfall variability, and drought frequency and intensity, amongst other factors. The De Martonne aridity index is particularly useful to evaluate the spatial and temporal variations in aridity in agricultural regions for characterising the climate of these areas and evaluating their susceptibility to climate change. From the mean precipitation and maximum–minimum daily temperature values recorded at 108 weather stations over 32 years (1989–2020) in Extremadura (southwest Spain), spatial analysis of aridity was performed at different grapevine growth stages. The present study aimed to (1) determine the mean aridity conditions in Extremadura according to year and growth stage and (2) assess aridity in six grapevine-growing areas of Ribera del Guadiana de Extremadura (Spain) protected designation of origin (PDO). To visualise aridity patterns, maps were generated using a geographic information system and a multivariate regression geostatistical algorithm (ordinary kriging). The climate of Extremadura is primarily Mediterranean at the annual scale, and aridity widely varies from extremely humid at the dormancy stage to arid at the berry development and ripening stages. This variation shapes the conditions of the studied grapevine-growing region. Furthermore, large differences were noted amongst the sub-areas of the Rivera del Guadiana PDO at the initial and final grapevine growth stages, requiring differential crop management. In addition, analysis according to growth stage allowed us to identify the most vulnerable areas and periods to climate change and potential grapevine-growing areas highly suitable for this climate.

Grapevine (Vitis vinifera) responses to salt stress and alkali stress: transcriptional and metabolic profiling

BackgroundSoil salinization and alkalization are widespread environmental problems that limit grapevine (Vitis vinifera L.) growth and yield. However, little is known about the response of grapevine to alkali stress. This study investigated the differences in physiological characteristics, chloroplast structure, transcriptome, and metabolome in grapevine plants under salt stress and alkali stress.ResultsWe found that grapevine plants under salt stress and alkali stress showed leaf chlorosis, a decline in photosynthetic capacity, a decrease in chlorophyll content and Rubisco activity, an imbalance of Na+ and K+, and damaged chloroplast ultrastructure. Fv/Fm decreased under salt stress and alkali stress. NPQ increased under salt stress whereas decreased under alkali stress. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment showed the differentially expressed genes (DEGs) induced by salt stress and alkali stress were involved in different biological processes and have varied molecular functions. The expression of stress genes involved in the ABA and MAPK signaling pathways was markedly altered by salt stress and alkali stress. The genes encoding ion transporter (AKT1, HKT1, NHX1, NHX2, TPC1A, TPC1B) were up-regulated under salt stress and alkali stress. Down-regulation in the expression of numerous genes in the ‘Porphyrin and chlorophyll metabolism’, ‘Photosynthesis-antenna proteins’, and ‘Photosynthesis’ pathways were observed under alkali stress. Many genes in the ‘Carbon fixation in photosynthetic organisms’ pathway in salt stress and alkali stress were down-regulated. Metabolome showed that 431 and 378 differentially accumulated metabolites (DAMs) were identified in salt stress and alkali stress, respectively. L-Glutamic acid and 5-Aminolevulinate involved in chlorophyll synthesis decreased under salt stress and alkali stress. The abundance of 19 DAMs under salt stress related to photosynthesis decreased. The abundance of 16 organic acids in salt stress and 22 in alkali stress increased respectively.ConclusionsOur findings suggested that alkali stress had more adverse effects on grapevine leaves, chloroplast structure, ion balance, and photosynthesis than salt stress. Transcriptional and metabolic profiling showed that there were significant differences in the effects of salt stress and alkali stress on the expression of key genes and the abundance of pivotal metabolites in grapevine plants.

VyUSPA3, a universal stress protein from the Chinese wild grape Vitis yeshanensis, confers drought tolerance to transgenic V. vinifera.

VyUSPA3 from the Chinese wild grape Vitis yeshanensis interacts with ERF105, PUB24 and NF-YB3, and overexpression of the VyUSPA3 gene in V. vinifera cv. 'Thompson Seedless' confers drought tolerance. Drought is a major abiotic stress factor that seriously affects the growth and yield of grapevine. Although many drought-related genes have been identified in Arabidopsis and other plants, the functions of only a few of their counterparts have been revealed in grape. Here, a universal stress protein (USP) A from the Chinese wild grape Vitis yeshanensis, VyUSPA3, was identified and its function was subsequently characterized by overexpressing or silencing the VyUSPA3 gene in V. vinifera cv. 'Thompson Seedless' via Agrobacterium-mediated genetic transformation. After 21 d of the drought treatment, most leaves of the untransformed (UT) 'Thompson Seedless' lines wilted, yet UT lines were less damaged compared to the RNAi-VyUSPA3 lines, nonetheless, the OE-VyUSPA3 lines were mostly unaffected. Meanwhile, OE-VyUSPA3 lines showed smaller stomatal aperture, more developed roots, higher leaf relative water content, proline content, and antioxidant enzyme activities, as well as lower malondialdehyde, H2O2 and O2•- accumulation than UT lines, but this response pattern was reversed in the RNAi-VyUSPA3 lines. Besides, the transcript levels of four drought-related genes (RD22, RD29B, DREB2A, and NCED1) in OE-VyUSPA3 lines were greater than those in the RNAi-VyUSPA3 and UT lines. In addition, a yeast two-hybrid assay and a bimolecular fluorescence complementation assay confirmed that VyUSPA3 interacted with ERF105, PUB24, and NF-YB3, respectively. This study revealed that VyUSPA3 improved drought tolerance in transgenic grapevines possibly through interaction with the hormone signaling, ubiquitination system, ethylene-responsive element binding factor and nuclear factors.

Grapevine Growth and Berry Development under the Agrivoltaic Solar Panels in the Vineyards

Grapevine Growth and Berry Development under the Agrivoltaic Solar Panels in the Vineyards