Growth Of Common Carp Research Articles

The dependence of the growth of carp yearlings (cyprinus carpio) on the density of planting in cages in the conditions of the Karkidon reservoir of Uzbekistan

The effect of stocking density on the growth of common carp (Cyprinus carpio) in cages was studied. Common carp yearlings (22 g) from the pond hatchery were stocked at 30, 60, 90 ind./m3 (in triplicate). Fish were fed with “Huvalon siliao” aquafeed (Uzbekistan) (35% protein) with ratio 3% of the fish biomass per day. The growth rates of fish in different variants, both in absolute terms and in relative terms (growth rate, specific growth rate), were the same. Carp yearlings were brought from a pond fish nursery and planted evenly in cages. A week was allo-cated as a period of adaptation of fish to the conditions of cages, during this period they were fed at the rate of 3% of the fish biomass. At the beginning of the experiment (May 10) the fish were planted in 30, 60, 90 yearlings of carp in a cage (in triple repetition), the average individual weight of carp (w1, g) in each of the nine cages was 22 g.

Comparison of Growth of Common Carp, Cyprinus carpio Cultivated in and outside Cages in Earthen Ponds in Basrah, Iraq

The current experiment was conducted in one earthen pond (2500 m2) at Agricultural Research Station of the Aquaculture Unit, College of Agriculture at the University of Basrah, Al-Hartha District from 17 February to 10 June 2021. This experiment aims to investigate growth differences between common carp cultivated in small cages (2 x 3 x 2 meter) located inside earthen pond and those were released in the same pond. Effects of fish stocking density inside small cages were also investigated. Four stocking densities were used [100 fishes in cages 1 and 2 (T1); 150 fishes in cages 3 and 4 (T2); 200 fishes in cages 5 and 6 (T3); 250 fishes in cages 7 and 8 (T4); while 1600 fishes were stocked in the earthen pond (T5)]. Fishes were fed daily 3% of fish weight on commercial sinking pellets manufactured by Agricultural Consultant Office of the College of Agriculture. Total length and weight of fishes were measured at the beginning and at the end of the experiment, while subsamples of fishes were weighed periodically and daily feed changed after each weighing. Daily feed was divided into three meals, the first one was given early at the morning, the second one at mid-day and the third one at afternoon. Results of current experiment revealed best growth criteria in T1 where average final weight was 750.5 g, average weight increment was 454.6 g, average daily growth rate was 4.33 g/day and average feed conversion rate was 2.77. Some of these criteria showed significant differences (P≤0.05) between T1 and other treatments, while feeding conversion rate (3.33) was achieved by fishes reared outside cages in the pond. As a conclusion, it can be pointed out that growth criteria of fishes reared inside cages were better than fishes reared outside cages and fish stocking densities were not affecting growth criteria of fishes reared inside cages.

Effect of Enzyme Supplementation in Diet on Growth of Common Carp (Cyprinus carpio L.) juveniles in RAS

The purpose of this study was to determine the effect of supplementing the diet with Thepax Aqua (Aqualase) on the development and survival of juvenile common carp (Cyprinus carpio L.). Therefore, an eight-week nutrition experiment was carried out using the Thepax Aqura enzyme in the Department of Vertebrates, Marine Science Center, University of Basrah. This enzyme was supplemented at 1, 2, and 3% in the control diet. The maximum weight growth (3.9870 gm 0.1230) and Specific Growth Rate (0.4238 gm 0.0049) were seen in the fish fed with T1 diet, and these results were found to be statistically different (P<0.05) from those observed in the fish fed with T2 (2 %), T3 (3 %), and T0 (control) diets. This study also concluded that there was no significant effect (P≤0.05) for enzyme levels on the survival of juveniles carp fed with different dietary enzymes. Finally, the outcome of the current experiment showed that the 1% supplementation of Thepax Aqua to the food gave good growth and feed utilization of Juveniles of carp C.carpio.

Effects of dietary Astragalus Membranaceus supplementation on growth performance, and intestinal morphology, microbiota and metabolism in common carp (Cyprinus carpio)

A large number of studies have indicated that Astragalus Membranaceus (AM) possesses growth-promoting effects in animal husbandry. However, the function of AM in common carp growth performance has not meant fully evaluated. In this work, to investigate the potential effects of AM and Lactobacillus plantarum-fermented AM (FAM) on growth performance, intestinal function, and intestinal microenvironment of common carp, fish with an average weight of 10.10 ± 0.25 g were divided into seven groups, and fed daily with an additive-free basal diet (BD); 0.1%, 0.2%, and 0.4% (w/w) of AM (L-AM, M-AM, and H-AM); 0.1%, 0.2%, and 0.4% (w/w) of FAM (L-FAM, M-FAM and H-FAM) for an eight-week period. The results showed that incorporating L-AM or L-FAM in the diet significantly increased (P < 0.05) the final weight, weight gain ratio, and specific growth rate compared with the BD group, but significantly decreased (P < 0.05) the feed conversion ratio. In L-AM and L-FAM groups, the villus height was considerably increased (P < 0.05), and crypt depth remained unaffected. L-AM and L-FAM both changed the abundance of intestinal microbiota with strikingly different dominant microbiota. Dietary L-FAM supplementation increased the abundance of Firmicutes, Verrucomicrobia, Cyanobacteria, TM7, WS3, Chlamydiae, TM6, Fusobacteria, Spirochaetes, Acidobacteria, Gemmatimonadetes, Actinobacteria, Chloroflexi, [Thermi], and Nitrospirae, whereas decreased Bacteroidetes and Proteobacteria abundances. Intestinal metabolome analyses demonstrated that L-AM altered metabolic composition and function, which were linked to immunity and host metabolism. Furthermore, L-FAM showed superior beneficial metabolic effects than L-AM in fish. On growth performance improvement and intestinal microenvironment modulation, dietary L-FAM supplementation was superior to AM in common carp. We speculate that L-FAM may improve the intestinal morphology and microenvironment, which strengthens the growth of fish. In addition, FAM might be promising antibiotics’ alternatives for promoting fish growth and controlling pathogens that has immense economic value in aquaculture.

Effect of new filtration medias on water quality, biomass, blood parameters and plasma biochemistry of common carp (Cyprinus Carpio) in RAS

Base on the principle of developing a suitable aquaculture system that will fit the present climate and environmental challenges to enhance breeding and production in aquaculture. Against the backdrop of issues connected to water quality in recirculating aquaculture system which has negative impact on the fish comfort, feeding rate and blood biochemistry, this may later affect its growth performance and general wellbeing. Although, the rate of waste disintegration and the bacteria species present in the filter system determine the physicochemical parameters of the treated water, which further affect the biological characteristics of the fish. This study intends to reveal the effect of biological filtration media (wood coal, rice hulls and wheat hay) on water properties and its further effects on growth performance, plasma biochemistry, liver and gills histopathology of common carp in RAS. After the acclimation interval, 24 tanks were stocked with 8 fish average initial weight of 15.74 g. However, treatments were assigned to each tank at random sampling to make four treatments. The results showed a significant effect on Common carp growth and body biomass, for those with filter media compared to the control. Though, a better growth performance of Common carp culture in water exposed to wheat hay and rice hulls were recorded at significant level with lower FCR. Hence improvement in fish productivity was observed in the treatments compared to the control. Moreover, there are significant changes on cell counts, and plasma biochemistry parameters. In synopsis, the study reveals the benefits in using additional media such as wheat hay and rice hulls as a biological filter media to improve water quality and growth performance of common carp in aquaculture. The use of this new media should be encourage since the installation of this biological filter media is simple, affordable and can be retrofitted into existing systems, to increase water management and aquaculture productivity.

Germ cells are not essential for sexual dimorphism of gonads in common carp, C. carpio L.

Common carp is the fourth most cultivated fish species in the world. Sexually dimorphic growth of common carp makes its sex differentiation an important research topic. The main objective of this study was to investigate the role of germ cells in sex differentiation of common carp. We generated the germ cell-deficient common carp through knocking down dead end gene with a morpholino antisense oligonucleotide during the embryogenesis. In the common carp without germ cells, we found about half of the gonads (type I gonads) contain granulosa cells and theca cells, which were the same with somatic cells we observed in the wild-type ovaries. The remaining half of the gonads (type II gonads) contain Sertoli cells and Leydig cells, which were the same with somatic cells in the wild-type testes. Furthermore, the expression of sex differentiation related genes was detected in the gonads without germ cells. It was found cyp19a1a was mainly expressed in the type I gonads, and the cyp19a1a mRNA level is much higher in each type I gonad than in the type II gonad. While dmrt1 was mainly expressed in the type II gonads, and the dmrt1 mRNA level is much higher in each type II gonad than in the type I gonad. Our comprehensive analysis showed that the gonadal structure and expression of genes related with sex differentiation were sexually dimorphic in the germ cell-deficient carp. These findings are valuable for discovering the underlying mechanism of common carp sex differentiation which is crucial for cultivating monosexual carp for aquaculture.

Pengaruh tingkat yang berbeda dari substitusi tepung ikan dengan tepung darah pada pakan ikan mas Cyprinus carpio L. dalam wadah terkontrol

The purpose of this study was to determine the feasibility using of blood meal on the growth rate and nutrient utilization of C. carpio L., as well as feeding with various levels of blood meal inclusion. The results showed that the absolute growth of common carp in treatment 1 with a combination was 10% higher than the other treatments. The daily growth rate of common carp in treatment 1 (1,8% / day) was higher than other treatments. Likewise, with the feed change value, treatment 1 showed significantly different from other treatments. The combination of fish meal and blood meal showed significantly different results from the results of statistical tests

PENGARUH KOMBINASI PAKAN KOMERSIAL DENGAN DAUN KANGKUNG AIR (Ipomoea aquatica) TERHADAP PERTUMBUHAN IKAN MAS (Cyprinus carpio)

This study aimed to analyze the effect of commercial feed combination with water spinach leaves (Ipomoea aquatica) at different ratios on the growth of common carp (Cyprinus carpio). This research was conducted for 35 days, located in Seteluk Atas Village, Seteluk District, West Sumbawa Regency. The common carp seeds used were 5-7 cm in size from one brood. The container used was 16 liter in size with a density of 10 fish / treatment. The method used in this study was an experimental method using a completely randomized design (CRD) with five treatments and three replications, namely, P1: 100% commercial feed (control), P2: commercial feed + water spinach leaves (1+1), P3: commercial feed + water spinach leaves (1+2), P4: commercial feed + water spinach leaves (1+3), P5: 100% water spinach leaves. The data obtained were then calculated and analyzed by using analysis of variance (ANOVA). The results of this study indicated that the use of commercial feed combination with water spinach leaves can affect growth, feed efficiency and feed conversion of common carp but did not affect the survival of common carp. Provision of 100% commercial feed (P1) and combination of commercial feed with water spinach leaves at a ratio of 1:2 (P3) had the same effect in increasing the growth of common carp, namely 1.17 g and 1.11 g, respectively.

Effects of Essential Oil Derived from the Bitter Orange (Citrus aurantium) on Growth Performance, Histology and Gene Expression Levels in Common Carp Juveniles (Cyprinus carpio).

The aim of this study was to detect effects of bitter orange (Citrus aurantium) essential oil, commonly called neroli oil (NO) (0, 0.25, 0.50, 1, and 1.5% referred to as NO0 NO0.25, NO0. 05, NO1 and NO1.5, respectively) on growth performance output and expression levels of some growth-related genes in the muscle tissue and some immune-related genes in the head kidney and pathological differences in digestive system organs of common carp Cyprinus carpio. The NO0.25 group had a large improvement in growth efficiency at the end of the 60-day feeding cycle. Real-time PCR (Bio RAD, USA) system was used to detect variations in gene expression levels. Furthermore, NO supplementation of up to 0.25% in muscle tissue controlled the release of growth hormone (GH) and insulin-like growth factor I (IGF-I). Furthermore, in the NO0.25 treatment category, immune response gene levels TNF-α, IL-8 and IL-1ß increased in head kidney tissue. In the histological examination of the liver and intestine, there were significant differences between fish fed with N1 and N1.5 diets. This study confirms that dietary supplementation of NO up to 0.25% can improve common carp growth efficiency and increase the expression of genes (GH and IGF-I) related to muscle growth, TNF-α, IL-8 and IL-1ß genes related to immune status, and liver and intestine histological status of common carp.

Effect of different organic manures and salinity levels on greenhouse gas emission and growth of common carp in aquaculture systems

A study was conducted to evaluate the effect of three different organic manures and salinity levels on production of greenhouse gases (GHGs) and their interactive effects on growth and physiological parameters of common carp (Cyprinus carpio) in peri-urban aquaculture tank system. The experiment had 3 × 3 factorial design with three types of organic manures, that is humic acid, FMGY (fermented boiled rice, molasses, groundnut oil cake and yeast) and cow dung tested in combination with three different salinity levels, that is 0.2, 2.5 and 5.0 g/L in triplicates. C. carpio fingerlings (average weight and length of 7 g and 7.15 cm respectively) were distributed to 27 number of 1000-litre FRP tanks (nine treatments with triplicates) containing 10 fish per tank. Fish were maintained for an experimental period of 180 days with a diet containing 30% crude protein. GHG estimation was done on 120th, 150th and 180th days at 30-day interval by collecting gaseous sample following floating chamber method. Lowest GHG emission (917.73) CO2 equivalent (mg m−2 h−1) was recorded in T3 treatment (humic acid + 5 g/L salinity). Highest fish growth was evident in T2 treatment (humic acid with 2.5 g/L salinity). GaSI and HSI were higher in treatment T2. Lowest stress parameters (plasma glucose and serum cortisol) were recorded in T4 treatment (FMGY with 0.2 g/L salinity). In terms of GHG emission, growth and bio-indices in C. carpio tank rearing, humic acid is the best organic manure at less than 5 g/L water salinity.

Interactive role of Wnt signaling and Zn in regulating testicular function of the common carp, Cyprinus carpio

Wnt signaling is conserved among all species and plays a significant role in various cellular processes including reproduction. The present study identified significant involvement of wnt4a, wnt5b, and wnt8a signaling in the testicular growth of common carp,Cyprinus carpio. Predominant expression of wnt4a, wnt5b, and wnt8a was found in the gonads and Wnt4a was localized in spermatocytes and interstitial cells. Ontogeny and testicular phase-wise analysis signified the importance of wnt isofoms analyzed in this study. Specific pathway activation of Wnt signaling revealed that Wnt4a and Wnt5b act through non-canonical while Wnt8a prefers the canonical pathway. The Wnt signaling regulates several steroidogenic enzyme and testis-related genes which was confirmed by the Wnt blockade experiments. Incidentally, zinc (Zn) is an essential trace element involved in the progression of spermatogenesis in teleosts. In adult male carp, a single administration of Zn at different doses elevated the expression of Wnt and Zn transporter genes and a single-dose (30 μg/g body weight [BW]) of Zn treatment elevated steroidogenic enzyme and testis-related genes which coincided with elevated androgens. Conversely, single-dose administration of Zn chelator to the Zn administered (30 μg/g BW) fish reversed the effects emphasizing a prominent role of Zn in the testicular function perhaps through Wnt signaling. Similar effects were observed in the in vitro experiments using the Zn chelator. Bioaccumulation of Zn and histological analysis revealed the importance of Zn in progression of spermatogenesis and sperm motility. Various assays related to cell viability and proliferation exhibited the role of Zn in promoting spermatogenic cell progression. Flow cytometric analysis confirmed Zn-induced elevation of Wnt and Zn transporter genes in germ and supporting cells. Furthermore, the effects of Zn are dose-related in carp. Taken together, it seems wnt4a, wnt5b, and wnt8a play an important role in testis and exposure of Wnt inhibitor, canonical as well as non-canonical activators, and Zn confirmed that Zn regulates Wnt signaling vis-à-vis promoting spermatogenesis in the common carp.

The Regulatory Role of Apelin on the Appetite and Growth of Common Carp (Cyprinus Carpio L.).

Simple SummaryFood intake and growth are two interrelated physiological processes. Fish obtain energy from the outside to meet their own growth and development through food intake. Apelin, as a new endocrine factor, plays a wide range of biological roles. Among them, apelin’s role in regulating appetite and growth has attracted the attention of researchers. Based on our investigations, in vitro and in vivo experiments showed that Pyr-apelin-13 could induce significant changes in mRNA levels of appetite-related and growth-related genes, suggesting that Pyr-apelin-13 may regulate the appetite and growth of common carp by regulating the expression of these key genes.Apelin, a kind of active polypeptide, has many biological functions, such as promoting food intake, enhancing immunity, and regulating energy balance. In mammals, studies have indicated that apelin is involved in regulating food intake. However, there are relatively few studies about the regulatory effect of apelin on fish feeding, and the specific mechanism is not clear. Therefore, the purpose of this study was to preliminarily investigate the regulatory effects of apelin on key genes of feeding and growth in common carp (Cyprinus Carpio L.) through in vitro and in vivo experiments. In the present study, after incubation with different concentrations of Pyr-apelin-13 (0, 10, 100, and 1000 nM) in hypothalamic fragments, the expressions of Neuropeptide Y (NPY) and Agouti related peptide (AgRP) mRNA were significantly up-regulated at 12 and 3 h, respectively, and the significant down-regulation of Cocaine and amphetamine-related transcript (CART) mRNA expression was observed at 1 and 3 h. In vivo, after Pyr-apelin-13 oral administration (0, 1, 10, and 100 pmol/g), the orexin mRNA level in the hypothalamus of common carp was significantly increased at 1, 6, and 12 h, while CART/(Proopiomelanocortin) POMC mRNA levels in the hypothalamus of common carp were significantly down-regulated. Following incubation with different concentrations of Pyr-apelin-13 (0, 10, 100, and 1000 nM) in primary hepatocytes, GHR (Growth hormone receptor), IGF2 (Insulin-like growth factor 2), IGFBP2 (Insulin like growth factor binding protein 2), and IGFBP3 (Insulin like growth factor binding protein 3) mRNA levels were significantly increased at 3 h. In vivo, the levels of IGF1 (Insulin-like growth factor 1), IGF2, IGFBP2 (Insulin like growth factor binding protein 2), and IGFBP3 mRNA were significantly increased after the oral administration of Pyr-apelin-13 in the hepatopancreas, in a time and dose-dependent manner. These results support the hypothesis that Pyr-apelin-13 might regulate the feeding and growth of common carp through mediating the expressions of appetite- and growth-related genes. Overall, apelin, which is an orexigenic peptide, improves food intake and is involved in the growth of common carp.

Elite allele mining for growth rate traits in common carp ( Cyprinus carpio ) by association analysis

Growth is one of the important economic traits to be improved in the breeding of common carp, yet the genetic basis in it remains unclear. In this study, a GoldenGate assay containing 1,536 SNP was used to genotype a full-sib panel of 68 individuals of mirror carp Cyprinus carpio. Association studies were carried out between SNP and growth rate for three consecutive months of the panel. 15 loci significantly associated with growth rate were identified using the general linear model and the mixed linear model, which located in eight chromosomes (chr6, 8, 9, 11, 20, 28, 32 and 35) and four scaffolds (scaffold902, 28835, 29008 and 29015). The phenotypic variance of these associated loci ranged from 6.4% to 19.2%. The elite alleles were mined based on the average phenotypic allele effect (AAE) of each allele. 15 elite alleles were screened out with 6.60% higher than the average phenotypic allele and 16.88% higher than the non-elite ones among the four traits. Meanwhile, 15 candidate genes were scanned and annotated. The loci and elite alleles of growth rate identified may facilitate marker-based breeding for rapid growth in common carp.

Effects of dietary fern (Adiantum capillus-veneris) leaves powder on serum and mucus antioxidant defence, immunological responses, antimicrobial activity and growth performance of common carp (Cyprinus carpio) juveniles

A 56-day research was performed to examine the influence of graded levels (0 (control), 0.5, 1 and 2%) of Fern (Adiantum capillus-veneris) leaves powder (FLP) in diet on immune competence and growth of common carp (Cyprinus carpio, initial weight = 20 g). The serum total immunoglobulins content and lysozyme activity in the 1 and 2% FLP groups remarkably increased compared to the other groups (P < 0.05). The skin mucosal lysozyme activity enhanced with increasing dietary FLP level in a dose-response manner. Fish fed on the FLP-supplemented diets had higher skin-mucosal superoxide dismutase activity than the control (P < 0.05). However, serum antioxidant enzymes were not affected by dietary fern (P > 0.05). The serum bactericidal activity against human and fish pathogens increased with enhancing the FLP level in diet against Staphylococcus aureus, Escherichia coli (EHEC ATCC 43895), Escherichia coli (CI), Pseudomonas aeruginosa, Klebsiella pneumonia and Aeromonas hydrophila. The serum antibacterial activity against Yersinia ruckeri in the 2% FLP group was higher than the other treatments. Furthermore, the serum bactericidal activity against P. aeruginosa (ATCC 27853) only observed in fish fed on the 1 and 2% FLP-supplemented diets. The skin mucosal bactericidal activity and inhibitory effects increased with enhancing the FLP level in diet against E. coli, K. pneumonia, Y. ruckeri and A. hydrophila in a dose response manner. Moreover, the skin mucosal bactericidal activity against S. aureus only observed in fish fed on 1 and 2% FLP-supplemented diets. The weight gain values in the 1 and 2% FLP groups were higher than the other treatments (P < 0.05). Feed conversion ratio (FCR) improved with increasing FLP level in diet in a dose-response manner (P < 0.05). By considering serum and mucosal bactericidal activities against different pathogenic bacteria, the supplementation of 2% FLP in diet is recommended for C. carpio during grow-out phase.

Otolith Microchemistry of Common Carp Reflects Capture Location and Differentiates Nurseries in an Interconnected Lake System of the North American Midwest

AbstractThe Common Carp Cyprinus carpio is an invasive fish whose populations have grown to ecologically damaging levels in the North American Midwest. It has been shown that some shallow nursery habitats contribute disproportionally to population growth of Common Carp in interconnected Midwestern lake systems. The ability of managers to discriminate which shallow basins are producing Common Carp across these systems could increase the efficacy of management practices aimed at disrupting recruitment. We tested whether adult and juvenile Common Carp collected across an interconnected Midwestern lake system could be distinguished based on the concentrations of 11 trace elements measured in their otoliths using laser ablation and high‐resolution inductively coupled plasma mass spectrometry (n = 157). Elemental concentrations in water were also measured with inductively coupled plasma mass spectrometry and found to differ among sites and between habitats, indicating that variation in otolith microchemistry was likely. Aluminum, barium, copper, iron, potassium, lithium, magnesium, sodium, phosphorus, and strontium in the edge region of otoliths differed significantly among Common Carp from all capture sites. Barium, iron, lithium, manganese, and phosphorus differed significantly among juvenile Common Carp from three nursery basins. Aluminum, barium, copper, iron, potassium, lithium, sodium, phosphorus, and strontium were significantly different between nursery and nonnursery habitats. We then investigated the ability to use these elemental otolith edge signatures to differentiate capture locations and distinguish between locations that are active and putative nursery habitats. Quadratic discriminant analysis could classify otoliths to collection site with an accuracy of 54%. Quadratic discriminant analysis had increased accuracy when restricted to juvenile Common Carp (76%) and when classifying Common Carp to nursery and nonnursery habitat types (87%). Further analysis of otolith and water microchemistry indicates temporal instability and suggests a need to collect a multiyear library of otoliths prior to utilization by managers.

Comparision of growth of mature all-female and mixed-sex Common carp (Cyprinus carpio L.) stocks in RAS

The common carp (Cyprinus carpio L.) is the most important fish species in Hungary, it is more than 70% of the total Hungarian fish production. The common carp production is important not only just in Hungary but in Middle-East Europe, as well as Southeast Asia. Majority of the production comes from fishpond culture. If the production sector wants to meet the increasing customer demands, there is need to intensify research on the intensive fish production opportunities for example all-female common carp technologies. The all-female technology is one of a genom-manipulation technology. Its production showed better growth rate than mixed-sex population in pond culture. Our experiment combined the recirculation aquaculture system (RAS) and the all-female common carp stocks intensification technologies. The reason for the experiment, is that there are no result about the growth of all-female common carp growth in RAS. &#x0D; The experiment used the „Tatai grey scale type” common carp stocks. We propagated two all-female stocks (T2 and T3) and a control group (TK). Due to technological characteristics of RAS, the water quality parameters were the same for all treatments and corresponded to the technological tolerance of common carp.&#x0D; The experimental period was from July 10, 2019 – November 20, 2019. Result of growth performance showed that the growth of mixed-sex stock was significantly higher than all-female stocks; (Control=3692.0±590.5g, T2=3438.8±415.4g, T3=3294.1±659.1g). Feed conversation ratio (Control=1.3±0.1 T2=1.5±0.2 T3=1.6±0.5) and SGR% (Control=0.8±0.0 T2=0.7±0.1 T3=0.7±0.1) were similar. &#x0D; By the results it can be said the all-female common carp technology has neither advantages nor disadvantages compared to the mixed-sex stock. The all-female technology can be beneficial if the consumers need female common carps. It is worth continuing the experiment and examine how the stocks will perform above 3kg body weight.

Effects of partial replacement of fishmeal with blood meal and dried porcine soluble on the growth, body composition and intestinal morphology of Cyprinus carpio

A 12-week feeding trial was conducted to assess the impact of fishmeal replacement with blood meal or dried porcine solubles on the growth performance, body composition and intestinal morphology of common carp (Cyprinus carpio). Fishmeal was 50% substituted with either blood meal or dried porcine solubles, or two-third substituted with both alternative products, at a 1:1 ratio. There was no significant difference in survival, final weight, weight gain ratio, feed conversion ratio or condition factor between the control and experimental groups (p > .05), although the visceral somatic index of the blood meal group was higher than that of the control group (p < .05). Crude lipid content was significantly higher in the dried porcine soluble group than that in the control group (p < .05), while other nutrient levels were not significantly different (p > .05). The amino acid composition of each experimental group was not significantly different from that of the control (p > .05). The intestinal villus height and fold depth of each experimental group were significantly lower than those of the control group (p < .05). The results of this study show that two-third fishmeal substituted with both blood meal and dried porcine solubles, at a 1:1 ratio, is the optimal fishmeal replacement for common carp growth.

Dietary olive (Olea europaea) leaf extract suppresses oxidative stress and modulates intestinal expression of antioxidant- and tight junction-related genes in common carp (Cyprinus carpio)

The present study was conducted in order to evaluate the effects of various levels of dietary olive leaf extract (OLE) on growth performance, whole body lipid peroxidation, and intestinal expression of hsp70 and antioxidant- and tight junction-related genes in common carp. Common carp juveniles (15 g) were fed with four diets, i.e. control and three diets supplemented with 0.1% (0.1E), 0.5% (0.5E) and 1% (1E) OLE for eight weeks. The results revealed that there was no significant difference in growth performance and feed efficiency between the control and OLE-treated groups (p > 0.05). There was no change in oxidative conditions among the treatments after one week, but the control group showed oxidative stress after eight weeks. The 0.1E and 0.5E treatments showed significantly suppressed oxidative stress (p < 0.05). After one week, there was no difference in sod gene expression among the treatments; cat showed down-regulation and up-regulation in the 0.1E and 1E treatments, respectively; gr showed up-regulation in the 0.5E and 1E treatments. After eight weeks, the 0.1E showed down-regulation; whereas, the 0.5E showed up-regulation in the antioxidant-related gene expressions compared to the control; the 1E treatment showed diverse changes in the gene expressions at this time. The 1E treatment had significantly lower hsp70 gene expression compared to the control after one week; however, after eight weeks, the 0.1E and 0.5E treatments showed significant down-regulation in the gene expression compared to the control and 1E treatments. Compared to the control group, the 0.1E (occl, cld3 and cld7) and 0.5E (occl and cld3) treatments showed up-regulation in tight junction-related gene expressions after one week. After eight weeks, there was no significant difference in occl and cld3 gene expressions among the treatments, but cld7 showed significant up-regulation along with the increase in dietary OLE supplementation. It is concluded that dietary OLE had no noticeable influence on common carp growth performance, but modulated gene expression of antioxidant enzyme and attenuated oxidative stress after 8 weeks.

PENGGUNAAN SISTEM AKUAPONIK DENGAN JENIS TANAMAN YANG BERBEDA TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN MAS (Cyprinus Carpio)

Common carp (Cyprinus carpio) is one type of freshwater fish that develops very rapidly as a commercial fish. The purpose of this study was to determine the best plant species in aquaponic systems in increasing optimal growth of common carp (Cyprinus carpio). This study used a completely randomized design (RAL) with 4 treatments and 3 replications. Analysis of variance (ANOVA) was used to see significant differences in treatment. Composition of treatment K (without plants), A (kale), B (lettuce), C (pakcoy). The main observation variables include absolute weight growth, absolute length, daily growth rate, survival rate and feed conversion ratio. The results of variance analysis (ANOVA) showed very significant differences in absolute length and survival variables. Absolute weight, daily growth rate and FCR in the study did not show any real differences. The highest absolute weight growth, daily growth rate, and feed conversion ratio are perlakaun K (without plants) (1,19)g, (1,59)cm and (3,48)g. The highest absolute length is treatment C (pakcoy) which is (0.97)cm. A good survival rate is B (lettuce) treatment, that is (60.00%).

Differences in energy utilisation efficiencies of digestible macronutrients in common carp (Cyprinus carpio) and barramundi (Lates calcarifer)

This study aimed to assess macronutrient-specific energy utilisation efficiency (i.e., protein, lipid and carbohydrate) for growth in common carp (an omnivorous species) and barramundi (a carnivorous species) and to assess if species-specific differences exist in energy efficiency of digestible protein (dCP), digestible fat (dFat) and digestible carbohydrates (dCarb). This was achieved by conducting a feeding trial experiment on common carp and by re-analysing data of a recent study on barramundi. A total of four diets were formulated following a 2 × 2 factorial design with 2 dCP-to-dFat ratios and 2 dCP-to-dCarb ratios. For carp, 2 feeding levels were applied such that the overall experimental design was a 2 × 2 × 2 factorial design, however for barramundi, three feeding levels were applied (satiation, 80% initial satiation and 60% initial satiation), resulting in a 2 × 2 × 3 factorial design. For each fish species, multiple regression of retained energy (RE) as a function of dCP, dFat and dCarb (in g.kg-0.8.d−1) was applied to estimate the energy utilisation efficiency of each digestible macronutrient. For carp, dCP, dFat and dCarb show linear relationships to RE, however for barramundi, dCP and dFat were linearly related to RE, but dCarb was curvilinearly related to RE. The estimated energy efficiencies of dCP, dFat and dCarb (respectively, kNE;dCP, kNE;dFat, and kNE;dCarb) for energy retention were 47, 86 and 60%, respectively, showing large degree of similarity with Nile tilapia and pigs. Carp and barramundi had similar kNE;dFat (86 vs. 94%), but different kNE;dCP (47 vs. 64%) and kNE;dCarb (60 vs. 18%). The net energy equations were NE = 11.2 x dCP + 34.1 x dFat +10.4 x dCarb for carp, and NE = 15.9 x dCP + 35.2 x dFat +9.4 x dCarb – 1.9 x (dCarb)2 for barramundi.