Female Group Research Articles

Blood-based biomarkers for early frailty are sex-specific: validation of a combined in silico prediction and data-driven approach.

Frailty is characterized by loss of physical function and is preferably diagnosed at an early stage (e.g., during pre-frailty). Unfortunately, sensitive tools that can aid early detection are lacking. Blood-based biomarkers, reflecting pathophysiological adaptations before physical symptoms become apparent, could be such tools. We identified candidate biomarkers using a mechanism-based computational approach which integrates a priori defined database-derived clinical biomarkers and skeletal muscle transcriptome data. Identified candidate biomarkers were used as input for a sex-specific correlation analysis, using individual gene expression data from female (n = 24) and male (n = 28) older adults (all 75 + years, ranging from fit to pre-frail) and three frailty-related physical parameters. Male and female groups were matched based on age, BMI, and Fried frailty index. The best correlating candidate biomarkers were evaluated, and selected biomarkers were measured in serum. In females, myostatin and galectin-1 and, in males, cathepsin B and thrombospondin-4 serum levels were significantly different between the physically weakest and fittest participants (all p < 0.05). Logistic regression confirmed the added value of these biomarkers in conjunction with age and BMI to predict whether the subjects belonged to the weaker or fittest group (AUC = 0.80 in females and AUC = 0.83 in males). In conclusion, both in silico and in vivo analyses revealed the sex-specificity of candidate biomarkers, and we identified a selection of potential biomarkers which could be used in a biomarker panel for early detection of frailty. Further investigation is needed to confirm these leads for early detection of frailty.

Temporal trends in age and stage-specific incidence of colorectal cancer in Saudi Arabia: A registry-based cohort study between 1997 and 2017

BackgroundIn Saudi Arabia, colorectal cancer (CRC) is the most common cancer in men and the third in women, posing a significant health burden. A comprehensive report of CRC incidence rates and trends in Saudi Arabia is lacking. This study aims to examine trends in CRC incidence among the Saudi population. MethodsWe used data from the Saudi Cancer Registry to examine CRC age-specific incidence rates (ASIR) and age-standardized incidence rates (ASR) between 1997 and 2017. Joinpoint regression analysis was used to determine the magnitude and direction of observed trends stratified by age, sex, and CRC stage at diagnosis. Trends were measured using the annual percentage change (APC) and the average annual percentage change (AAPC) in CRC incidence rates. ResultsIn total, 19,463 new CRC cases were identified during the study period. Since 1997, ASR for CRC has steadily increased in men and women overall, irrespective of disease stages. The ASIR increased across all age groups and was more pronounced in older patients. Women aged 40–49 had a higher increase in incidence than men (AAPC= 5.3 % vs.4.7 %). Males aged 70–79 had an AAPC of 10.2 %, twice that of females (AAPC= 4.9 %). A consistent rise in ASIR was observed across all CRC stages and age groups in males and females. In recent years, males under 50 had a higher APC for distant CRC than females, while females aged 50–74 experienced a steeper increase in distant CRC than males. ConclusionWe report a marked increase in the incidence of CRC over time in Saudi Arabia, affecting men and women across all age groups and disease stages at diagnosis. Our findings underscore the need to identify underlying risk factors and to develop and implement effective prevention policies and strategies, including screening programs to facilitate early detection and treatment.

Enhancing interpretability in random forest: Leveraging inTrees for association rule extraction insights

The random forest model is a powerful supervised learner, recognized for its ability to learn the pattern within data with superior predictive accuracy. However, it is a black box model because it lacks interpretability. This study addressed the interpretable challenge by employing the inTree framework. The rules were extracted from each decision tree in a random forest model, and the association rules were determined through measured matrix support and confidence to reveal the frequent variable interactions for predicting unemployment. This approach provided insight into the relationships between specific variables and unemployment outcomes. The developed method used data set from the integrated labor force survey (ILFS) 2020/2021 in Zanzibar. Zanzibar’s unemployment rate consistently increased across surveys conducted in 2006, 2014, and 2020/2021. Results have shown that the rules that most predict unemployment for individuals are female and lack of health insurance and secondary education level, female and youth age group and lack of health insurance and secondary education level with a high confidence level. This study provides practical insights for Zanzibar’s government to develop effective interventions, programs, and policies. Improving the interpretability of the random forest model enhances decision-making to address unemployment challenges.

Healthcare Monitoring-based Internet of Things (IOT)

Remote health care is needed when direct medical monitoring is unavailable. Modern technology provides several ways to make patient access simpler. In particular, cloud, IoT, and data mining technologies have been successful in health care and medicine. This method identifies illnesses using data collected from two groups of patients, a male group and a female group where data are based on the heart rate, systolic blood pressure, and body temperature parameters. The three classifier types use the collected data: the naïve Bayes, support vector machine (SVM), and the J48. These three classification methods were considered to measure the classification accuracy using the proposed healthcare IOT-based monitoring system. The Precision, Recall, and F-measure evaluation measurements were used to quantify the obtained accuracy results of the two groups. The obtained results showed excellent classification results of 0.96, 0.99, and 0.98 for the naïve Bayes classifier to the male group that outperformed the results of the other two classifiers for the precision, recall, and f-measure, respectively. As for the female group, similar results have been obtained. In conclusion: The use of the Internet of Things in the proposed system makes it accurate and easy to use remotely by patients.

Gender difference in the relationship between epicardial adipose tissue and central obesity.

Body fat distribution is closely related to cardiovascular disease than the amount of total body fat itself. The epicardial adipose tissue (EAT) measured by transthoracic echocardiography represents central obesity. We hypothesized that the gender affected the link between EAT thickness and body fat distribution. We measured EAT thickness using transthoracic echocardiography and total body fat and regional body fat distribution using dual-energy X-ray absorptiometry (DXA) in 390 patients (250 males, 59 ± 11 year-old). The EAT thickness was measured on the free wall of the right ventricle at the end of the diastole on parasternal long-axis view of echocardiography. The median and mean EAT thickness of 390 patients were 4.0 mm and 4.2 ± 1.2 mm, respectively. The mean EAT thickness revealed positive correlation with truncal fat mass to total body fat mass ratio (FMtrunk/FMtotal, R = 0.291, P = .024). Subgroup analysis categorized by gender demonstrated the female group has stronger correlation of EAT thickness with fat distribution than the male group (male: R = 0.174, P = .006, female: R = 0.378, P < .001). EAT thickness is closely related to body fat distribution. Females showed a more significant correlation between EAT thickness and central fat accumulation than males. EAT thickness might be considered as a relevant parameter of central obesity, especially in females.

Psoas Sarcopaenia Outcomes in Elderly Patients After Acute Lower Gastrointestinal Bleeding.

Introduction Sarcopaenia has been examined as a predictor of frailty in surgical patients and may predict mortality. The hypothesis of this study is that sarcopaenia is associated with an increased risk of death following an episode of acute lower gastrointestinal bleeding (ALGB). Methods This study included patients admitted with ALGB between January 2017 and June 2022, who underwent cross-sectional imaging (CSI). CSI was accessed via imaging platforms, and the total psoas area (TPA) was calculated at the third lumbar vertebra (L3) by a radiology resident (post-Fellowship of the Royal College of Radiologists(FRCR)) and validated by a consultant radiologist. Clinical, mortality, and demographic data were obtained from the Northern Ireland Electronic Care Record (NIECR). Psoas muscle index (PMI) was calculated using TPA, standardising for height in mm2/m2. Sarcopaenia was defined asPMI<545 mm2/m2for males and <385 mm2/m2for females. Results A total of 103 patients were included. Of these, 32 patients were defined as sarcopaenic (male, n = 20; female, n = 12). There was a statistically significant increase in mortality in the sarcopaenic group(Chi square (1, N = 103 = 7.582, p = 0.005888), odds ratio (OR) of 3.34, 95% confidence interval (CI) of 1.41-7.91). The Kaplan-Meier curve showed a significant decrease in survival probability in combinedmale and female groups (p < 0.001). There was a statistically significant association between sarcopaenic patients and length of hospital stay (LOHS) compared with non-sarcopaenic patients (p < 0.001). Conclusions Sarcopaenia as a predictor of frailty is an important risk factor for all-cause mortality in ALGB. CSI provides an opportunity to identify as well as investigate the aetiology of ALGB. Although radiation risk may limit its use, it should be considered when available.

A Cross-Sectional Study on the Association between Physical Activity and Cognitive Function Among Community-Dwelling Older Adults in Tianjin.

The association between physical activity (PA) and cognitive function remains controversial, and the impact of gender on this association remains underexplored. Therefore, this study aimed to investigate the association between PA and cognitive function and to explore whether this association was modified by gender among older adults. In 2016, a cluster sampling method was used to select community-dwelling older adults aged 65 and above. PA was assessed using the International Physical Activity Questionnaire-Short Form and classified as low, middle, and high. Cognitive function was assessed by using the revised Chinese version of the Wechsler Adult Intelligence Scale. The multiple linear regression model was used to explore the association between PA and cognitive function and to assess whether this association differs by gender. A total of 676 participants with a mean age of 73.63 ± 6.39 were included. The multiple linear regression analysis showed that higher PA was significantly statistically associated with Full Intelligence Quotient (FIQ), Performance Intelligence Quotient (PIQ), and verbal Intelligence Quotient (VIQ) (P<0.05). Among the WAIS-RC subtests, higher PA was significantly statistically associated with higher scores of the similarity subtest, picture completion subtest, and picture arrangement subtest (P<0.05). In the gender subgroup analysis, higher PA was significantly statistically associated with FIQ and PIQ (P<0.05), but no significant association was found with VIQ (P>0.05) in the male group, while in the female group, there was no significant statistical association between higher PA and FIQ, PIQ, or VIQ (P>0.05). Higher PA was significantly statistically associated with better cognitive function (P<0.05). In the male group, PA was significantly statistically associated with cognitive function, whereas no comparable association was found in the female group.

Effects of Integrating Stair Climbing-Based Exercise Snacks Into the Campus on Feasibility, Perceived Efficacy, and Participation Perspectives in Inactive Young Adults: ARandomized Mixed-Methods Pilot Study.

This 6-week pilot study aimed to investigate the feasibility, perceived efficacy, and participation perspectives of a university campus stair climbing-based exercise snack (ES) intervention and to compare it to moderate-intensity continuous training (MICT). Healthy, young, inactive adults (age: 21.6 ± 2.3 years, BMI: 22.5 ± 3.6 kg/m2) were randomly assigned to three groups (ES, MICT, and Control [CON]). ES (n = 14, 6 females) and MICT (n = 13, 9 females) groups performed three sessions per week over 6 weeks, while the control group (n = 15, 9 females) maintained their habitual lifestyle. ES involved 3 × ~30 s 'all-out' stair-climbing (6 flights, total 126 steps and 18.9 m elevation) bouts separated by > 1 h rest, and MICT involved 40 min stationary cycling at 60%-70% HRmax. Mixed linear effect models were used to explore within and between-group differences, and the change in magnitude was determined using mean difference (MD), 95% confidence interval (CI), and Cohen's d effect size. Fourteen participants also completed post-intervention semi-structured interviews. Retention rates of 93% (ES, 14/15), 93% (MICT, 13/14), and 100% (CON, 15/15) were achieved from baseline to post-assessments, with compliance (participants' attendance to scheduled ES sessions) of 97% (733/756 sessions) and 93% (217/234 sessions) in ES and MICT, respectively. No significant differences were found between ES and MICT for future intentions (4.8 ± 1.4 and 5.0 ± 0.9), enjoyment (PACES, 89.0 ± 16.4 and 94.5 ± 11.9), and satisfaction. A significant group × time interaction was found in the quality of life and perceived stress. ES and MICT significantly increased the quality of life by 8% (MD = 5.5 [95% CI = 0.3, 10.7], Cohen's d = 0.64) and 6% (MD = 4.6 [95% CI = 0.9, 8.2], Cohen's d = 0.88) compared to baseline, whereas CON experienced a significant decrease (MD = -7.9 [95% CI = -14.4, -1.6], Cohen's d = 0.56). ES significantly decreased perceived stress by 7% (MD = -4.6 [95% CI = -8.9, -0.3], Cohen's d = 0.58), while MICT (MD = 2.0 [95% CI = -0.1, 4.1], Cohen's d = 0.28) and CON (MD = -2.4 [95% CI = -6.1, 1.3], Cohen's d = 0.22) had no significant effects. The majority (12/14) of participants generally demonstrated positive attitudes toward participation in ES and found it easier than expected while highlighting enablers, barriers, and future recommendations. Stair climbing-based ES could be integrated into the campus setting, appeared feasible, and was similar to MICT regarding future intentions, enjoyment, and satisfaction. Both ES and MICT might induce relevant positive effects on the quality of life, and ES demonstrated greater benefits compared to MICT or CON for reducing perceived stress. Interviews provided positive insights into fostering adherence to ES among university students.

Safety evaluation of alpha-glycerylphosphorylcholine as a novel food

To evaluate the safety of alpha-glycerylphosphorylcholine (α-GPC) as a novel food, the study of acute oral toxicity, subchronic toxicity, teratogenic toxicity and genotoxicity were conducted. In acute oral toxicity, no toxic effects were observed in rats of both genders administrated 10.0 g/kg BW α-GPC. In 90-day oral toxicity, female high-dose group (2,000 mg/kg) had lower body weight, body weight gain, empty stomach body weight, total protein (TP), albumin (ALB), and higher alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) in contrast to control group. In teratogenic toxicity, the body weights of pregnant rats on the 9th day (d9), the 12th day (d12), the 15th day (d15), and the 20th day (d20), body weight gains, and net body weight gains in high-dose group (2,000 mg/kg) decreased, the other parameters had no difference compared to control group. In genotoxicity tests (Mammalian erythrocyte micronucleus, Chromosome aberration and Ames test), all dose groups didn't display significant change compared with negative control group. Based on above results, α-GPC is actually low hazard novel food, has a NOAEL of 1,000 mg/kg BW for female rats and 2,000 mg/kg BW for male rats following 13-week oral exposure, has a NOAEL of 1,000 mg/kg BW for pregnant rats and 2,000 mg/kg BW for fetal rats in teratogenic toxicity, has no genotoxicity in vitro or in vivo.

Species behavioral characteristics lead to unique fragmentation threats: The Nubian ibex as a case study

AbstractThe behavioral characteristics of species may result in certain populations being inherently more susceptible to fragmentation. For example, species exhibiting spatial sexual segregation or those constrained to elongated and narrow habitats. We studied the fragmentation threats, spatial dynamics, resource utilization, and movement ecology of a particularly vulnerable species that is both sexually segregated and constrained to elongated and narrow habitat—the north Judean Desert population of Nubian ibex (Capra nubiana). From 2016‒2020 we tracked 48 marked ibex (27 male, 21 female), of which 38 (20 male, 18 female) also had global position system (GPS) collars. Using GPS‐collar and camera‐trap data in zones delineated around perennial water sources (PWSs), we calculated ibex drinking frequencies and individual utilization distributions by season and sex, focusing on their overall (95% isopleth) and core (50% isopleth) home ranges. We quantified joint space use between sexes using a utilization distribution overlap index (UDOI) and ibex daily movements and space use via movement indices. Female groups formed philopatric activity centers that were anchored around PWSs year‐round and arranged in a metapopulation‐like structure, with no female movement detected between them. Conversely, movement of adult males changed seasonally, with the cores of male groups anchored around PWSs only during the dry season, and long‐range movement between female activity centers during the rut. Female groups also spent more time at steeper terrain and higher elevations compared with male groups. Outside the rut, groups of males and groups of females exhibited minimal joint space use (i.e., average dry season UDOI was 0.06). These patterns indicate high sensitivity of this population to intersexual fragmentation by obstacles (physical or virtual). Management strategies to mitigate fragmentation threats for such populations should be sex‐specific and landscape‐oriented.

Comparison between pancreatojejunostomy and pancreaticogastrostomy in pancreaticoduodenectomy in a gastroenterology and hepatology teaching hospital

Background: Pancreatoduodenectomy (PD) is a surgical procedure used to treat diseases of the pancreatic Head, the distal common bile duct (CBD), and the duodenum, as well as trauma to the head of the pancreas. Once the pancreatic head and the duodenum are resected, the surgeon needs to reconnect the stomach, the biliary tract, and the pancreatic stump. The technique for reconstructing the biliary tract is fairly standard, represented by the anastomosis of the CBD to the jejunum, but the reconstruction of the pancreatic stump is a matter of debate. In general, two procedures can be used: pancreaticojejunostomy (PJ), which is an anastomosis between the pancreatic stump and the jejunum, and pancreaticogastrostomy (PG), which is an anastomosis between the pancreatic stump and the stomach. Aim: The objective of this study was to compare the impact of PJ and PG on the occurrence of postoperative pancreatic fistula (POPF), morbidity, and mortality following PD. Patients and Methods: This study took place between April 1, 2021 and April 1, 2023 at Gastroenterology and Hepatology Teaching Hospital in Baghdad Medical City. The cases in the study were designed prospectively. It is a comparison between PG and PJ after undergoing Whipple surgery. A total of 30 patients were selected and divided into two groups. The first group consisted of 16 patients who underwent PJ, while the second group consisted of 14 patients who underwent PG. The cases were prepared in the surgical consultation clinic. On the one hand, through PG, it was determined whether the texture of the pancreas was soft and the size of the main pancreatic duct (MPD) was less than 3 mm. On the other hand, through PJ, it was determined whether the texture of the pancreas was firm or hard and the size of the MPD was more than 3 mm. Results: The age group of males and females ranged from 11 to 68 (median 57) years. There were a total of 16 males and 14 females. The ampullary tumor was present in 11 patients of the PJ group and 7 patients of the PG group. Histopathology of tumors showed adenocarcinoma in 13 patients of the PJ group and in 6 patients of the PG group. POPF occurred in 3 patients of the PJ group, type A in 1 patient and type B in 2 patients, and no POPF was observed in the PG group. Hemorrhage occurred in 1 patient of the PG group and 2 patients of the PJ group. Delayed gastric emptying occurred in 5 patients: 3 patients of the PJ group (type A) and 2 patients of the PG group. SSI (surgical site infection) occurred in 7 patients: 5 patients of the PJ and 2 patients of the PG group. Finally, mortality occurred in 3 patients of the PJ group. Conclusion: Pancreatic intestinal anastomosis must be tailored to the surgeon's preferences and experience level, and any innovative technique that reduces the prevalence of POPF is welcome in pancreatic surgery. PG seems to be associated with less POPF, morbidity, and mortality than PJ after undergoing Whipple surgery, especially given the soft texture of the pancreas and the size of the MPD &lt; 3 mm. Recommendations: 1. Because our study included a limited number of samples, further prospective randomized studies with larger samples are required to confirm that PG had fewer complications. 2 Surgeons should be encouraged to use PG more frequently in the future.

Insufficient capacity to cope with stressors decreases dietary quality in females

BackgroundPrevious studies had found the effects of stress on eating behaviors. The aim of this study was to explore the effects of perceived stress on dietary quality by sexes in Chinese adults.MethodsThe study population consisted of individuals aged 18 to 59 years participating in the China Health and Nutrition Survey. Perceived stress levels were measured using the 10-item Perceived Stress Scale (PSS-10), with scores ranging from 0 to 40. Dietary quality was evaluated by Chinese Dietary Guidelines Index (CDGI) ranging 0-110. Using a two-level mixed effects model with community as level 2 and individual as level 1, the study analyzed the impact of PSS-10 scores on CDGI. Additionally, a two-level mixed effects structural equation model was employed to explore the effects of distress factor scores and coping stressor factor scores on dietary quality among different sexes within the population.ResultsThis study involved in 2515 males and 3165 females, and the average age was 45.9 ± 9.6.The PSS-10 score and CDGI were 14.6 ± 5.1 and 44.8 ± 11.6, separately. After adjusting for confounders, there were no effects of PSS-10 score, distress factor score, and coping stressors factor score on CDGI in males. After adjusting for confounders, CDGI in the highest tertile of PSS-10 score group was 1.64 lower than that in the lowest tertile group in females(P = 0.001). The distress factor score was not associated with CDGI (standardized β=-0.164, P = 0.488), while the coping with stressors factor score was significantly negative with CDGI (standardized β=-0.834, P < 0.001) in females. CDGI in the highest tertile of coping with stressors factor score group was 4.36 lower than that in the lowest tertile group (P < 0.0001).ConclusionsThere was no association between perceived stress and dietary quality in adult males. The perceived stress, especially the insufficient capacity to cope with stressors, was negatively associated with dietary quality in adult females.

Single-leg horizontal jump is more suitable for assessing lower limb function compared to single-leg vertical jump in patellofemoral pain patients

BackgroundThe single-leg vertical jump (SLVJ) and single-leg horizontal jump (SLHJ) are commonly used assessments for individuals with lower limb injuries. Despite previous studies comparing these tasks across various individuals, their comparative effectiveness in evaluating lower limb function in individuals with patellofemoral pain (PFP) and any potential sex differences in these functional tests remain uncertain. Research questionThe purpose of this study was to determine which jump task is more suitable for functional assessment in PFP patients by comparing the differences in jump performance and biomechanical variables between PFP patients and healthy individuals during the SLVJ and SLHJ. Furthermore, the study aimed to determine whether the more suitable functional assessment for male and female PFP patients is consistent. MethodsA total of 24 PFP patients (15 males, 9 females) and 17 healthy individuals (9 males, 8 females) were recruited. Three-dimensional kinematic and kinetic data were collected during the propulsion and landing phases of the SLVJ and SLHJ. Two-way ANOVA was used to compare each dependent variable between groups (PFP vs. healthy controls) and sex (male vs. female). ResultsBoth male and female PFP groups presented a reduced jump distance than the control group in the SLHJ (p = 0.002), but no significant difference was detected in the SLVJ. Both male and female PFP groups displayed increased hip work (p = 0.005) and contribution (p = 0.009) and reduced knee work contribution (p = 0.034) during the propulsion phase of the SLHJ compared to the control group. Moreover, female participants of PFP and control groups performed shorter jump distances (p ≤ 0.001) and had less knee work (p < 0.001) during the propulsion phase of vertical and horizontal jumps than males. ConclusionSingle-leg horizontal jump is more suitable for assessing lower limb function in PFP patients, characterized by reduced knee work contribution impacting jump performance, and this applies to both male and female PFP patients.

Effects of different types of music on spatial memory in mice

Abstract. A preliminary investigation was conducted to find out whether different types of music have an effect on spatial memory in adult mice. Thirty mice were divided into six groups, each half male and half female, namely, female pop group, male pop group, female classical group, male classical group, female control group, male control group, and kept in cages and fed freely. There was a reduction in latency time in the musical group compared to the control group. There was a significant difference between the classical group and the control group (P&lt;0.05), while there was no significant difference between the pop group and the control group (P&gt;0.05). There was an enhancement of spatial memory in mice by classical music and no statistically significant enhancement of spatial memory in mice by popular music.

Factors of Fake News Sharing in the Context of the Ukraine-Russia Conflict: The Effect of Suspicions About European Unions' Intentions.

This study examines the separate and joint effects of potential factors of fake news dissemination. A sample of 359 Romanian participants (61.3% female), aged between 18-79years (M = 45.01; SD = 15.69) filled questionnaires assessing their perceptions of conflict between European Union (EU) and Ukraine; fake news sharing, and various socio-demographic data. Our results revealed that the behaviour of sharing fake news is positively associated with age and more prevalent in men. Also, people who perceive a conflict between EU and Ukraine and thus suspect that behind the EU's consistent support for Ukraine lies a hidden interest are more inclined to spread fake news. In the female group, age was found to moderate the relationship between perceptions of conflict between EU and Ukraine and fake news sharing. We also discuss the relevance of our findings for public communication efforts aiming to mitigate the dissemination of fake news.

The Impact of User Experience and Emotional Response on the Purchase Behavior of Chinese Consumers Within the Context of the She-economy

Abstract: With the rise of the "She -economy," female consumers' attention to and willingness to purchase national products are increasing. This paper focuses on the female consumer group, exploring their user experience and emotional responses during the process of purchasing national products, and how these factors affect their willingness to buy national products. The paper collects relevant data through a questionnaire survey and uses SPSS 27.0 for statistical analysis of the data. The results show that female user experience and female emotional response have a significant positive effect on purchase intention, with female emotional responses playing an important mediating role. This paper enriches the research content of the "She-economy" theory and provides new insights for understanding female consumer behavior. At the same time, the research results have practical significance for both enterprises and female consumers.

Humanin-G Ameliorates Hemorrhage-Induced Acute Lung Injury in Mice Through AMPKα1-Dependent and -Independent Mechanisms.

Background/Objectives: The severity of acute lung injury is significantly impacted by age and sex in patients with hemorrhagic shock. AMP-activated protein kinase (AMPK) is a crucial regulator of energy metabolism but its activity declines with aging. Humanin is a mitochondrial peptide that exerts cytoprotective effects in response to oxidative stressors and is associated with longevity. Using a mouse model of hemorrhagic shock that mimics the clinical condition of adult patients, we investigated whether treatment with a humanin analog, humanin-G, mitigates lung injury and whether its mechanisms of action are dependent on the catalytic AMPKα1 subunit activation. Methods: Male and female AMPKα1 wild-type (WT) and knock-out (KO) mice (8-13 months old) were subjected to hemorrhagic shock by blood withdrawal, followed by resuscitation with shed blood and lactated Ringer's solution. The mice were treated with PEGylated humanin-G or vehicle and euthanized 3 h post-resuscitation. Results: Sex- and genotype-related differences were observed after hemorrhagic shock as lung neutrophil infiltration was more pronounced in the male AMPKα1 WT mice than the female WT mice; also, the male AMPKα1 KO mice experienced a significant decline in mean arterial blood pressure when compared to the male WT mice after resuscitation. The scores of histological lung injury were similarly elevated in all the male and female AMPKα1 WT and KO mice when compared to the control mice. At molecular analysis, acute lung injury was associated with the downregulation of AMPKα1/α2 catalytic subunits in the WT mice, whereas an increased activation of the signal transducer and activator of transcription-3 (STAT3) was observed in all the vehicle-treated groups. The in vivo administration of humanin-G ameliorated histological lung damage in all the groups of animals and ameliorated mean arterial blood pressure in the male AMPKα1 KO mice. The in vivo administration of humanin-G lowered lung neutrophil infiltration in the male and female AMPKα1 WT mice only but not in the KO mice. The beneficial results of humanin-G correlated with the lung cytosolic and nuclear activation of AMPKα in the male and female AMPKα1 WT groups, whereas STAT3 activation was not modified. Conclusions: In adult age, hemorrhage-induced acute lung injury manifests with sex-dependent characteristics. Humanin-G has therapeutic potential and the AMPKα1subunit is an important requisite for its inhibitory effects on lung leucosequestration, but not for the amelioration of lung alveolar structure or the hemodynamic effects of the peptide.

Analysis of successful and unsuccessful snatch and clean and jerk lifts in IWF World Championships (2011–2023)

In this research, male and female athletes who won gold, silverand bronze medals at the International Weightlifting Federation Senior World Championship (between 2011 and 2023); (a) analysis of successful and unsuccessful lifts in snatch and clean and jerk techniques, (b) analysis of successful and unsuccessful lifts in 3 lift attempts in both techniques and, (c) It is aimed to investigate the lifting attempts of athletes that determine their medal rankings in snatch, clean and jerk and total. In this retrospective study, data of 3144 (1603 and 1541 for male and female, respectively) individual results obtained from the lifting performances of 528 athletes (male n = 270; female n = 258) participating in the Senior World Championship (between 2011 and 2023) in snatch and clean and jerk were analyzed. In the study, the frequencies and rates of the individuals included in the study according to different variables are presented with descriptive statistics. Various Chi-square analyzes were conducted to determine differences in distribution rates between groups. The significance level in the analyzes was determined as p < .05. It was determined that the successful lifts ratios of the athletes who achieved gold, bronze, silver medals in the light weight, middle weight and heavy weight categories of the male and female groups were higher than the unsuccessful lifts ratios in both lifting techniques (excluding silver medals in clean and jerk in lightweight for men; except bronze medals in snatch in lightweight for women). It was observed that the percentage rates in the unsuccessful lifts of athletes who achieved gold medals in both snatch and clean and jerk of the male and female groups were lower than the percentage rates in the unsuccessful lifts of athletes who took part in silver and bronze medals. It was observed that there was no significant difference between the distribution rates of successful and unsuccessful lifts distribution rates according to gender in both snatch and clean and jerk of both genders. It was observed that the successful lifts rates in both the snatch and clean and jerk of the male and female groups decreased from the 1st attempt to the 3rd attempt, and the lifting rates in the unsuccessful lifts increased. It has been observed that the lift attempts that determine the medal rankings of the groups in snatch, clean and jerk and total are the 2nd attempt and 3rd attempt. The findings can provide a comprehensive perspective on the in-competition factors that contribute to World Championship success in order to directly inform coach and athlete strategies. These findings may also be valid for national or regional champions and may include other strength-based sports with a similar structure.

Effect of acute exercise on gene expression in peripheral blood mononuclear cells of puberty children

Studies have shown that acute exercise alters the expression of circulating neutrophil and peripheral blood mononuclear cell (PBMC) genes, affecting a wide range of processes from cellular growth to the promotion of inflammatory and anti-inflammatory responses. In this study, we identified differential gene expression associated with acute exercise and PBMC in puberty children through the GEO database. We utilized bioinformatics technology to analyze the impact of acute exercise on PBMC and identified key genes involving the puberty children. Combining two datasets of gene expression, we identified 197 shared differentially expressed genes (DEGs) in PBMC before and after exercise. We conducted pathway analysis of these DEGs and built a gene co-expression network. The identification of hub genes was achieved through the utilization of protein-protein interaction (PPI) networks. 268 shared DEGs were identified in the GSE11761 dataset in pre- and post-exercise groups, while 352 differential genes were identified between the pre- and post-exercise groups of females in the GSE14642 dataset. A total of 197 shared genes were identified between the two datasets. Seven core genes were identified by MCODE and CytoHubba, namely IL2RB, KLRD1, GZMB, TBX21, PRF1, HAVCR2, and GZMA. Bioinformatics techniques are effective in screening and analyzing PBMC-associated DEGs in children undergoing acute pre- and post-exercise puberty. Microarray analysis of PBMCs in children undergoing puberty can identify molecules that may predict acute exercise responses. This study provides insights for further exploration of the mechanisms and targets of acute exercise on the organism.

Exploring eating disorder risk among Thai transfeminine youth: a comparative study with cisgender females

BackgroundAdolescents, particularly vulnerable during puberty, often face heightened concerns about dieting and body image. Transfeminine youth, especially in Asian cultures, are understudied in this context. This study investigates eating disorder risks among Thai transfeminine youth and compares to cisgender females, focusing on the association with body dissatisfaction. This study aims to explore 3 objectives: (1) to assess and compare the prevalence of eating disorder risks between Thai transfeminine youth and cisgender females; (2) to examine the link between body dissatisfaction and eating disorder risks; (3) to identify risk factors associated with abnormal eating disorder screening outcomes.MethodThis is a cross-sectional comparative study conducted between September 2021 and October 2022. The study questionnaire includes Thai version of the Eating Attitude Test-26 (EAT-26) to assess the risks of eating disorders and Thai version of the Body Image Concern Inventory (BICI) to assess body dissatisfaction. The questionnaire was distributed through social media platforms to transfeminine and cisgender females, aged 12–25 years. The correlation between eating disorder risks and body dissatisfaction was analyzed with linear regression, while the comparisons between transfeminine individuals and cisgender female groups were analyzed with Wilcoxon rank-sum test.ResultsThe study included 241 transfeminine individuals and 388 cisgender females, median age 19.0 years (interquartile range [IQR] 18–21) and 18 years (IQR 16–20), respectively. Ninety (37.3%) transfeminine individuals had an EAT-26 score ≥ 20, suggesting concerns for dieting and eating behaviors, compared to 70 (18%) cisgender females (p < 0.001). Risk factors for EDs included obesity (odds ratio [OR] 3.22, confidence interval [CI] 1.36–7.6) and low income (OR 3.66, CI 1.59–8.4). Protective factors were negligible exercise (OR 0.24, CI 0.09–0.66), gender identity disclosure (OR 0.27, CI 0.1–0.77), and high levels of happiness (OR 0.85, CI 0.73–0.98). There is a significant correlation between the positive eating behavior screening and the body dissatisfaction scores.ConclusionsTransfeminine youths show significantly higher concerns about dieting, body weight, and eating behaviors than their cisgender females, highlighting the unique challenges they face. These results emphasize the needs for targeted screening, health care access for treatment and support for eating disorders in transfeminine youths.