In laboratory, the highest mortality rates of Tetranychus urticae after the use of metabolic products of symbiotic bacteria with a titer of 1 × 107 were observed in Xenorhabdus bovienii at 6-8 days post application (dpa) in the experiment with live and at 8 dpa of autoclaved culture (about 95%). In experiments with live and autoclaved culture with a titer of 1 × 107, the mortality mites at 8 dpa in X. bovienii was almost the same, but in X. nematophila it was slightly higher in autoclaved culture. At 8 dpa, the efficacy of the live and autoclaved metabolic products of Xenorhabdus bovienii and X. nematophila against the spider mite with a titer of 1 × 105 was about 1.4 times lower compared to the culture with a titer of 1 × 107. The relationship between the mortality of spider mites (%) and the exposure time (days) to bacterial metabolism products most reliably reflects by the polynomial dependence with the accuracy of approximation 0.93-1.0. In the greenhouse, the effectiveness of the bacterial metabolic products of X. bovienii against spider mite was highest in experiments with live culture with a titer of 1 × 108. In experiments with live culture of X. bovienii with a titer of 1 x 107 (in vivo) the mortality rate of spider mites on leaves of shrub Dracaena sanderiana at 8 dpa increased from 84% on the ground floor to 90% on the second floor. The overall efficacy of the bacterial metabolic products of X. bovienii (in vivo, titer 1 × 107) against adults, larvae and nymphs of T. urticae on the leaves of perennial marsh grasses ( Potenderia cordata , Thalia geniculata and T. dealbata ) was about 98-99%.