Under severe pandemic conditions, any airborne microbe, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), leads to increased fatality if physical screening is compromised. Therefore, an investigation was carried out to design a reusable screening mask that could promise the highest safety and be compatible with users. After going through the analytical and clinical investigation, the design and the work quality are confined to the ground version. To initiate top quality mask screener design of high-tech gold nanoparticles (AuNPs) hydroxyethyl methacrylate (HEMA) composite mask, safety evaluation data from all masks were collected. The porous polyvinyl chloride (PVC) circular grid frame was coated with a gold metallic nano particles layer, and the flexible adhesive polymeric matrix mesh plasticizers polyethylene terephthalate (PET) was used to coat bed on each grid, as a design. In-vitro quantitative analysis process assembly of bio-contaminant and specific matter was developed, in which breathed/vacuumed air was passed via fractional screening cone adhesive edged stumbling block route for final pure contaminant free air. The vacuumed air was collected through the adhesive slide, and microbial growth and particular matter were analysed and discovered to be best compared to conventional filter masks. Gold coat film entrapment tests with colony density were performed through Fourier transform infrared spectroscopy (FTIR), and virus crystal structure analysis was done through an X-ray diffractometer. Clinical investigations of this development were done with 50 volunteers. The research demonstrates the advantages of the AuNPs HEMA composite reusable mask design comparatively best over other mask designs regarding microbiological screening and lab safety, and it may be helpful to stand against biological warfare.
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