Greece Introduction Research Articles

POSTER ABSTRACTS

POSTER ABSTRACTS

Succession in megafaunal assemblages of an artificial reef during a three-year monitoring in the Eastern Mediterranean basin

Succession in megafaunal assemblages of an artificial reef during a three-year monitoring in the Eastern Mediterranean basin

Spelling in a case of logopenic variant of primary progressive aphasia

Spelling in a case of logopenic variant of primary progressive aphasia

A cost comparison between gluten – free and conventional foods

A cost comparison between gluten – free and conventional foods

The consumption of dietary supplement which contains plant extracts affects superoxide dismutase activity in healthy adults

The consumption of dietary supplement which contains plant extracts affects superoxide dismutase activity in healthy adults

Changes in body composition and Basic Metabolic Rate (BMR) following bariatric surgery

Changes in body composition and Basic Metabolic Rate (BMR) following bariatric surgery

Comparison of wild greens and herbs consumption between residents of urban and rural areas of Crete

s / Clinical Nutrition ESPEN 13 (2016) e55ee74 e61 less information exist concerning the effect of the MD on the secondary prevention of CHD. Aim: The aim of this study is to investigate the effect of MD on the risk of cardiovascular event onset and cardiovascular mortality in patients who have suffered an Acute Coronary Syndrome (ACS). Materials and methods: A perspective, multicenter study of ten yearfollow-up (GREECS-Greek Study of ACS) was conducted (2004-2014). In the study 2.172 individuals (76% male) with ACS were enrolled from 6 hospitals in urban and rural Greek regions. In the information obtained anthropometric and socio-demographic characteristics, lifestyle habits, nutritional and psychological assessment and medical history were included. To assess the degree of adherence to the MD, the MedDietScore tool (range: 0-55) was used. The statistical analysis included: a) analysis of descriptive characteristics and b) development of multiple logarithmic regression models. Results: Greater adoption of the MD was associated with lower risk for fatal cardiovascular event (OR: 0,979, 95%CI: 0,960-0,998) after adjustment for age and sex. However, the statistical significance of the Mediterranean dietary pattern was attenuated (OR: 0,977, 95%CI: 0,953-1,002) when traditional cardiovascular risk factors were added to the model. The factor that seems to affect the relationship of the MD with cardiovascular mortality is physical activity. The level of adherence to the MD was not associated with the onset of a new cardiovascular event. Conclusions: The adoption of the MD reduces the risk of a fatal cardiovascular episode, without affecting the risk of next cardiovascular event in patients with ACS. COMPARISON OF WILD GREENS AND HERBS CONSUMPTION BETWEEN RESIDENTS OF URBAN AND RURAL AREAS OF CRETE Chrysoula Kaloteraki , Maria-Eleni Velivasaki , Eleni Tsourdalaki , Antonios Koutelidakis . Department of Nutrition-Dietetics, Technological Educational Institute of Crete, Siteia, Greece; Department of Food Science and Nutrition, University of the Aegean, Mirina, Limnou, Greece Introduction: Wild greens and herbs of Crete were an integral part of traditional Cretan diet, providing numerous bioactive components important for reduction of diseases risk and for health promotion. Aim: To investigate the frequency of wild greens and herbs consumption and a comparison between urban and rural areas of Crete (Countries of Heraklion and Rethymnon). Materials and methods: 200 men and women volunteers, aged between 18 and 70 years old, completed a questionnaire about the frequency of wild greens and herbs consumption. Half of them were residents of Heraklion city, while the rest of them were residents of the mountain villages of Rethymno County (Melambes, Anogia, Kamariotis). Then, a comparison of the frequency of consumption between the two populations, as well as correlations with nutritional and behavioral factors, was carried out. The results were processed with the SPSS v.17 statistics program through the use of correlation factors.T-test and one-way ANOVA were applied. Results: The village residents eat more often wild greens and herbs (2-4 times per week), compared with city residents (1-2 times per month) (p<0.05). The most often consumed greens are purslane and capers, while the most often consumed herbs are chamomile, sage and dittany. Increased consumption of greens and herbs in rural areas was associated with increased physical activity (mainly rural employment) and a reduced incidence of smoking and coffee consumption. Conclusion: The nutrition assessment with purpose to increase the frequency of consumption of wild greens and herbs from urban residents can play an important role towards improving health, via possible degenerative diseases prevention. CORRELATION BETWEEN APOE ALLELES AND OBESITY MARKERS IN ADULTS Alexandra-Aikaterini Koulouri , Dimitrios Loukovitis , Nikolaos Rodopaios , Eleni Vasara , Tatiana Serafeim , Maria Tsigga , Maria Chasapidou , Antonios Kafatos . Department of Social Medicine, Preventive Medicine and Nutrition Clinic, University of Crete School of Medicine, Greece; 2 'Lab of Agrobiotechnology and Inspection of Agricultural Products, Department of Agricultural Technology, Alexander Technological Educational Institute of Thessaloniki, Greece; 3 Lab of Animal Physiology, Dept of Zoology, School of Biology, AUTH, Greece; Department of Human Physiology, School of of Nutrition & Dietetics, Alexander Technological Educational Institute of Thessaloniki, Greece; Department of Nutrition & Dietetics, School of of Nutrition & Dietetics, Alexander Technological Educational Institute of Thessaloniki, Greece; 6 424 G.M.H., Thessaloniki,

Study of possible beneficial effect of crocin in an animal model of diabetes

s / Clinical Nutrition ESPEN 13 (2016) e55ee74 e65 Materials and methods: 107 patients diagnosed with cancer and treated in an oncology centre of excellence who have been referred to the community nursing home-care service. A pilot cross-sectional quantitative study, based on convenience sampling. The screening tool used to determine malnutrition was the ESPEN Nutrition Day questionnaire for oncological patients in greek language, as translated and validated by GrESPEN. Results: On average, a weight loss of 8 kilograms over the last year was noted for male patients, contrary to women, for whom no change was noted. In 76% of cases the weight loss was unintentional and 13% of the sample population has already lost more than 20% body weight. Minimum BMI was 19.8 with a good correlation to cancer stage. Risk factors for malnutrition identified included taste distortion, anorexia and constipation. None of the individuals at risk had been referred for nutrition counseling/support before the study. Conclusions: The community nurses should promote cancer patients’ education on nutritional status screening within the context of multidisciplinary nutrition teams. STUDY OF POSSIBLE BENEFICIAL EFFECT OF CROCIN IN AN ANIMAL MODEL OF DIABETES Konstantinos Feidantsis , Nantia Apostolidou , Eleftheria Galatou , Antigone Lazou . 1 Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, GR 54124, Greece; Alexander Technological Educational Institution of Thessaloniki, Department of Nutrition and Dietetics, Thessaloniki, Greece Introduction: Diabetes mellitus is a metabolic disease whose worldwide incidence is high (4-5%). The connection between diabetes with poor metabolic control and the high mortality due to cardiovascular disease, hyperlipidemia, nephropathies and neuropathies are very well established. Several recent studies demonstrate the protective effect of a wide variety of antioxidants to tissue damage as in the case of myocardial infarction. The extract of the plant Crocus sativus L. (commonly known as saffron) contains compounds, such as crocin, picrocrocin and safranal. Among the pharmacologically active ingredients, crocin is the most important and abundant antioxidant, has antitumor, antioxidant, anti-hyperlipidemic properties and is known to strengthen memory. Aim: The purpose of this study is the possible improvement of heart, liver and kidney function of diabetic rats after oral administration of crocin (10 and 20 mg / kg body weight) for 2 weeks and identification of the underlying molecular mechanisms that contribute to the improvement of cardiac, hepatic and renal function of the animals. Materials and methods: Specifically levels of biochemical indicators such as glucose, triglycerides, urea, total cholesterol, HDL and LDL and levels of enzymes SGOT, SGPT and GGT were detected in animal blood plasma. Results: a The study was carried out as part of a nutritional educational program that is performed from Aegean University (Responsible Prof.: Lecturer PD 407 Koutelidakis A.) in collaboration with 301 ARMY DEPOT (Responsible from 301 Depot: C. Z. Titakis, PhD candidate, Dept of Chemical Engineering, National Technical University of Athens) Figure 1. Glucose, triglycerides, urea, total cholesterol, HDL and LDL levels in control, diabetic and diabetic rats after the oral administration of crocin (10 and 20 mg/kg body weight). Values are means ± SD; n1⁄45 preparations from different animals. *P 0,05). Conclusion: The implementation of a nutrition education program with aim to improve dietary habits, may lead to improved work efficiency and promoting the health of workers. DEVELOPMENT AND VALIDATION OF A MODEL THAT ESTIMATES BODY FAT PERCENTAGE BASED ON SIMPLE ANTHROPOMETRIC MEASUREMENTS Vladlena Khudokonenko , Spyridon Kanellakis , Efstathios Skoufas , Eftychia Apostolidou , Loukia Gerakiti , Maria Andrioti , Christianna Georgopoulou , Georgia Ziogou , Theodora Makridi , Yannis Manios . Department of Nutrition and Dietetics, Harokopio University, Athens, Greece; Department of Nutrition and Dietetics, T.E.I. of Thessaly, Karditsa, Greece; Department of Nutrition and Dietetics, T.E.I. of Crete, Sitia, Greece

Towards medical education neuroscience: pilot results of integrative affective analytics from clinical skills workshops

Towards medical education neuroscience: pilot results of integrative affective analytics from clinical skills workshops

Viability and osteo/odontogenic differentiation of dental pulp stem cells in crosslinked chitosan-gelatin scaffolds

Event Abstract Back to Event Viability and osteo/odontogenic differentiation of dental pulp stem cells in crosslinked chitosan-gelatin scaffolds Maria Chatzinikolaidou1, 2, Athina Bakopoulou3 and Anthie Georgopoulou1 1 University of Crete, Materials Science and Technology, Greece 2 FORTH, IESL, Greece 3 Aristotle University of Thessaloniki, Department of Fixed Prosthesis and Implant Prosthodontics, Greece Introduction: Chitosan-gelatin scaffolds have been proposed due to their suitable mechanical, biodegradation and biocompatibility properties for different biomedical applications, including cartilage, skin and peripheral nerve regeneration [1][2]. Aim of this study was to investigate the potential of glutaraldehyde (GTA)-crosslinked chitosan-gelatin scaffolds to promote attachment and osteo/odontogenic differentiation of Dental Pulp Stem Cells (DPSCs) towards targeted dental tissue regeneration. Methods: Porous scaffolds were prepared by dissolving 2% (w/v) chitosan in a 1% (v/v) acetic acid solution and 2% (w/v) gelatin in ultrapure water at 50 oC. The two solutions were poured together to reach a composition of 40%-60% chitosan-gelatin and stirred for 2 h at 50 oC. GTA was then added to the mixture at 0.1% (w/v) under agitation at 50 oC until gelation. The gel mixture was then casted into 24-well plates and lyophilized for 24 h at -20 oC. Scaffolds were neutralized using 0.1 M NaOH and rinsed thoroughly with ultrapure water. DPSC cultures were established from third molars of young healthy donors and characterized for several stem cell (SC) markers with flow cytometry [3]. For in vitro cell culture, scaffolds were washed with PBS (3 times, 30 minutes each), followed by culture medium and again PBS (3 times, 30 minutes each). Finally, scaffolds were incubated with culture medium for 24 h at 37 oC. Each scaffold was then spotted with 100 μl complete culture medium (a-MEM containing 15% Fetal Bovine Serum-FBS, 100 mM L-ascorbic acid phosphate and antibiotics/antimycotics) containing 106 DPSCs (from passages 2-6). Spotted scaffolds were incubated for 2 h at 37 oC and 5% CO2 and then fully covered with 1.2 ml culture medium/well. Medium change was performed every 2 days. After 3, 7 and 14 days, live/dead fluorescent staining (Calcein M/PI staining) was used to assess cell viability by means of confocal microscopy. Moreover, total RNA was isolated after 7 and 14 days and real time PCR was performed to evaluate expression of osteo/odontogenic markers (BSP, DSPP, BMP-2, ALP, osterix, Runx2). The results were adjusted by amplification efficiency (LinRegPCR) and were normalized to the two most stable housekeeping genes evaluated by geNorm (SDHA and B2M). Results and Discussion: We prepared stable 40%-60% chitosan-gelatin scaffolds with interconnective pores and pore size of 100 μm. The established DPSC cultures were positive (>95% of the population) for SC markers, like CD90, CD73, CD29, CD81, CD49f, CD51, CD166, Nestin, Nanog, and partly for CD105, CD146, STRO-1, CD24, CD271, Oct3/4, SSEA-4. The 40%-60% chitosan-gelatin scaffolds could support high levels of cell attachment and viability over time. Real time PCR showed that cells grown inside the scaffolds presented a significant upregulation of DSPP [up to16-fold], BSP [up to 5.4-fold], BMP-2 [up to 2.6-fold], osteocalcin [up to 4.7-fold], osterix [up to 8.8-fold] and ALP [up to 1.5-fold]. Conclusion: These results indicate that the proposed 40%-60% chitosan-gelatin scaffolds support osteo/odontogenic differentiation of DPSCs even in absence of inductive media and therefore can be further considered for other properties, such as their ability to induce dentin or bone-like mineralized tissue formation to be used for targeted dental and periodontal tissue regeneration. This study was financially supported by the Hellenic General Secretariat for Research and Technology grant Aristeia II ‘Osteobiomimesis 3438’ and funded by the European Union (EU) and National Resources

Development and in vitro evaluation of bioinspired, chitosan based, 3D hybrid nanohydroxyapatite scaffolds.

Event Abstract Back to Event Development and in vitro evaluation of bioinspired, chitosan based, 3D hybrid nanohydroxyapatite scaffolds. Despoina Brasinika1, Athena Tsetsekou1, Kyriaki Kyriakidou2, Ioannis K. Karoussis2 and Triantafyllos Papadopoulos2 1 National Technical University of Athens, School of Mining and Metallurgical Engineering, Greece 2 National and Kapodistrian University of Athens, Faculty of Dentistry, School of Health Sciences, Greece Introduction: In the present work the synthesis of 3D hybrid porous scaffolds was performed through a freeze drying method, aiming at the development of implantable materials with enhanced biocompatibility and bioactivity. These hybrid materials constitute an innovative alternative for implant development, in order to successfully replace damaged human tissue and provide adequate mechanical stability till the natural regeneration of the desired tissue. The hybrid scaffolds consist of hydroxyapatite nanocrystals, synthesized biomimetically in the presence of natural biomolecules, in an effort to tailor the nucleation process and control the hydroxyapatite’s crystal growth. Since these scaffolds serve as a template to support and guide formation of new tissue, the material is required to present a porous network with high interconnectivity and mechanical stability[1],[2]. However, the porous structure of the scaffolds, inevitably affects the material’s mechanical properties. For this purpose, chemical cross-linking was performed with organic crosslinkers, through bonding with the biomolecules’ amino groups[3],[4]. Materials and Methods: Aqueous suspensions of hybrid nanohydroxyapatite were prepared through a bioinspired approach[5],[6] and then they were undertaken lyophilization to develop porous 3D scaffolds. To improve scaffolds’ mechanical properties, cross-linking of the biomolecules was performed prior to lyophilization. Finally, the physicochemical and biological properties of the as-obtained scaffolds were examined. Results: Evaluation of scaffolds’ internal porosity with Scanning Electron Microscopy (SEM) and X-ray computed microtomography (micro-CT) indicates the development of a highly interconnected porous network with bimodal pore size distribution (Fig. 1). Fig. 1: SEM images of the internal structure of hybrid scaffolds differing in the biomolecules employed for increasing mechanical stability. Optimization work provided scaffolds with pore size ranging from 50 to 100 μm for the large pores and around 5 to 10 μm for the smaller ones. Cell viability and cytotoxicity tests (MTT assay and fluorescein diacetate/propidium iodide staining (FDA/PI)) proved that after 7 days of cultivation a well-populated biomaterial was obtained with viable cells distributed through the scaffold (Fig. 2). Fig. 2: SEM image of osteoblasts adhered into the porous structure of a 3D scaffold after 7 days of cultivation. Discussion: The adopted bioinspired approach led to the fabrication of 3D hybrid porous scaffolds with a complex architecture and high interconnectivity among the pores. Their chemical composition and nanostructure - resembling that of natural bone - along with their complex porous network provide a cell friendly environment which proved beneficial for cell distribution, attachment and proliferation. Conclusions: The as-produced hybrid scaffolds comprise biocompatible biomaterials with promising properties for tissue engineering applications. The research leading to these results has received funding from National Strategic Reference Framework 2007–2013, Cooperation programs, Project BIOMINY – 09SYN-41-757

Measuring self-disgust in patients with Parkinson's disease: An emotion induction paradigm using the Skin Conductance Response (GSR)

Measuring self-disgust in patients with Parkinson's disease: An emotion induction paradigm using the Skin Conductance Response (GSR)

A Nanobots-based Methodology for Assessing the Acoustic Signal's Attenuation in the Auditory System's Pathway

Event Abstract Back to Event A Nanobots-based Methodology for Assessing the Acoustic Signal's Attenuation in the Auditory System's Pathway Maria Kalogeropoulou1, Panagiotis Katrakazas2*, Kostas Giokas2 and Dimitrios-Dionysios Koutsouris2 1 National Technical University of Athens, School of Electrical and Computer Engineering, Greece 2 National Technical University of Athens, School of Electrical and Computer Engineering, Greece Introduction: Our auditory system is designed to convert acoustic information from low-level sensory representations into perceptual representations. A full understanding of the mechanisms responsible for auditory streaming requires a straight comparison of neural activity and behavioral reports of the auditory perception. [1] During hearing loss however, the pathways carrying audio information from the cochlea to the auditory cortex are altered resulting from blockage or damage to peripheral auditory structures. [2] As it is important to determine the degree of hearing loss and how it is dependent upon the acoustics of the stimuli, experience, or task demands, we propose a methodology of assessing the attenuation of the acoustic signal by using several nanobots agents placed along the signal’s pathway and determining the acoustic reflex. Proposed methodology: As the acoustic reflex is a contraction of the middle-ear muscles induced by an intense auditory incitement, stimulation on either the ipsi- or the contralateral side should result in bilateral muscle contraction in a normal hearing system. However, in a case of sensorineural hearing loss, the acoustic reflex threshold is shown to be reduced by at least two different factors: the degree of synchrony of neural activity across frequency, and the fast-acting compression mechanism in the cochlea. [3] In our study, we aim to check via simulation the attenuation of the acoustic signal inside the ear channels, as the signal is still considered as "sound". This will be realized via the use of four (4) micro-sensors placed on the facial nerve (CN7) and vestibulocochlear nerve (CN8), which will obtain the corresponding electrical signal in order to identify the problem, in coordination with the nanobots agents. If a nerve is found to be problematic, a simple search method can be applied (e.g. the bisection method) to control the subinterval where the attenuating signal exists. In that case, the nanobots agents will move alongside the nerve to locate the problematic area. The aforementioned setup will be implemented via a computer-aided engineering software (e.g. Solidworks) with the use of the detailed anatomical model of the human head, MIDA v1.0 [4]. The navigation algorithm used in nanobots is described in our previous work [5], where acoustic waves will be used to minimally affect brain tissues. The anatomical parameters for the CN7 and CN8 nerves are taken from [6], defining the design parameters of the nanobots agents as well as their position alongside the nerves and the tympanic membrane. To verify the acoustic signal attenuation, probe microphone measurements (PMM) will be initially performed to assess the level of signal a hearing-loss patient receives near his/her tympanic membrane. The purpose of PMM is to ensure that appropriate gain is received by a person with hearing loss, where accurate hearing thresholds are converted from the audiogram measurement (dB HL) to the measurement of a hearing aid output (dB SPL). [7] That signal will be also received by the nanobots alongside the nerves, which will be used as a reference signal, while at the same time they will record the signal’s path alongside the nerves. The difference between a real-time measurement of the signal and the reference one will provide us with the level of attenuation that occurs throughout the acoustic path. That will give us a better insight to where the hearing loss occurs and provide us with a more holistic view of the acoustic signal’s path in order to better evaluate the levels of hearing loss and adjust hearing aid modifications, if necessary, so as to enhance the acoustic experience of the user. Figure 1 Acknowledgements This project has received funding from the European Union’s Horizon 2020 MSCA RISE Action PROPHETIC, under grant agreement No 644704.

Incidental and intentional memory performance in depression and amnestic mild cognitive impairment

Incidental and intentional memory performance in depression and amnestic mild cognitive impairment

A method for cross-task mental workload classification based on brain connectivity

A method for cross-task mental workload classification based on brain connectivity

479. Expression of the apoptosis inhibitors p53, bcl-2, EGFR and survivin in renal cell carcinomas: Correlation with pathological features and clinical outcome

S S181 479. Expression of the apoptosis inhibitors p53, bcl-2, EGFR and survivin in renal cell carcinomas: Correlation with pathological features and clinical outcome J. Contis, P. Lykoudis, C. Nastos, A. Karakatsanis, D. Dellaportas, A. Delis, D. Karandrea, A. Pafiti-Kondi 1 University of Athens Medical School, 2nd Dept. of Surgery, Athens, Greece University of Athens Medical School, Pathology, Athens, Greece Introduction: Survivin is a member of the inhibitors of the apoptosis (IAP) family. Over expression of survivin has been found in most human cancers and has been associated with poor prognosis. We studied the prognostic significance of the apoptosis inhibitors survivin, p 53, bcl-2, EGFR in renal cell carcinomas and correlated their expression with pathologic features and overall survival. Material and methods: 78 renal cell carcinomas were retrospectively assessed by immunohistochemistry for survivin, p53, bcl-2 and EGFR expression. Gender, age, size, histological type and grade of tumor as well stage were recorded, while survival data were retrieved from the follow-up records of the patients. Statistical analysis of the above data was carried out. Results: 52 male and 26 female patients aged from 35 to 87 years (median 66 years) were included.Mean tumor diameter was 6.8 cm and themost common location was the upper left kidney. Clear cell adenocarcinoma was observed in 77% of case, granular type in 14%, spindle cell type in 6% and papillary type in 3%of the cases. 16%of the tumorswere grade 1, 56%grade 2 and 18%were grade 3. Survivin expressionwas detected in 59 (76%) cases, bcl-2 in 64% of cases, p53 in 82% of cases and EGFR in 54% of cases. Statistical analysis showed that p53, survivin and tumor stage but not bcl-2 and EGFR status were significantly related to survival. Cox regression analysis showed that survivin expression was an independent prognostic factor for RCC and was associated with a poorer prognosis. Conclusions: Our study suggests that survivin is an independent prognostic factor of overall survival in renal cell carcinomas and this finding may indicate a need for adjuvant therapy for high-risk patients. No conflict of interest. http://dx.doi.org/10.1016/j.ejso.2014.08.485

P-085: Resolution of factor XI (FXI) deficiency in refractory ulcerative colitis (UC) after surgery: a case report

s of the 3rd International Symposium on Pediatric Inflammatory Bowel Disease S425 (perianal disease, ileal and/or colonic stenosis with or without abscess with respective resections). In multivariate analysis, no association with age, gender, inflammatory and serologic markers was detected. Conclusion: IBD is rare in our country, however extensive forms predominate and rates of surgery are high, especially in CD (40% by the final visit), with important resections, reflecting the severity of IBD. Family history and positivity of ANCA and ASCA are low; therefore, other factors have to be involved in this aggressive phenotype. P-084 Eosinophilic gastroenteropathy from diet to colectomy A. Karoliny1 *, J.B. Kovacs1, A. Nagy1, N. Lasztity1, K. Meichelbeck1, L. Karsza1, G. Hegyi1, B. Lestar2, G. Veres3, M. Lorincz1. 1Pal Heim Children’s Hospital, Budapest, Hungary, 2Hungarian Defence Forces Med. Center, Dept. of Surgery, Budapest, Hungary, 31st Dept. of Pediatrics, Semmelweis University, Budapest, Budapest, Hungary Background: Incidence of eosinophilic gastrointestinal disorders is increasing worldwide, however, precise pathomechanism, diagnostic criteria and therapy, in severe cases, is not well known. While there are well documented publications and guidelines about the clinical manifestation and treatment of eosinophilic oesophagitis the incidence, clinical manifestation and treatment of primary eosinophilic gastroenterocolitis is less well known. Case report: A 4-year-old male patient was presented with massive haematochesia and diarrhoea. Upper and lower endoscopy showed gastritis, duodenitis and ulcerative pancolitis, respectively. Histology confirmed eosinophilic gastroenteropathy (>100 eosinophils/HPF in duodenum and colon). 5-ASA, systemic corticosteroid, leukotriene receptor antagonist and azathioprine treatment combined with elimination diet did not achieve remission. Due to further deterioration and increased transfusion demand nasogastric feeding with amino acid based formula was introduced. After two years of unsuccessful conventional therapy infliximab was given, but due to severe bloody diarrhoea total colectomy was needed. After 12 months of follow-up, the patients is symptom free, growing well with no hematochezia. None of the upper and lower endoscopies, biopsies, and colectomy specimens showed any signs of Inflammatory Bowel Disease (IBD), but high number of eosinophils. Conclusion: IBD and eosinophilic colitis may share a common phenomenon resulting differencial diagnostic difficulties. Here, we presented a patient with severe form of eosinophilic gastroenteritis causes life-threatening hematochezia. Biological treatment and colectomy for this indication is rarely indicated. P-085 Resolution of factor XI (FXI) deficiency in refractory ulcerative colitis (UC) after surgery: a case report A. Angelakopoulou1 *, K. Lindley2, M. Mathias2, S. McCartney3, A. Windsor3, F. Kiparissi1. 1Great Ormond Street Hospital NHS Trust, London, United Kingdom, 2Great Ormond Street Hospital, London, United Kingdom, 3University College London Hospital NHS Trust, London, United Kingdom Introduction: Coagulation abnormalities in acute inflammatory conditions such as sepsis are well recognised. Chronic inflammatory conditions, such as Inflammatory Bowel Disease, have the potential to induce a coagulopathic or procoagulant state with potential devastating consequences. Aim: To report the resolution of severe FXI deficiency in severe UC post colectomy and restorative surgery. Methods: We present the case of a 14-year-old male with severe UC, FXI deficiency and an acute, thrombotic cerebrovascular event. Results: The patient presented with two-week history of headache, vomiting, altered consciousness and left leg weakness, one year after diagnosis of severe UC refractory to combination immunosuppressive therapy. MRI changes in keeping with Right Middle Cerebral Artery ischaemic stroke. Cerebral/visceral angiography excluded systemic vasculitis. Investigations revealed APTT of 67.3s (26 38s) and low FXI (13 IU/dl; 50 150). Platelet count showed a drop post stroke (lowest 76) with subsequent gradual recovery. Congenital FXI deficiency was excluded by patient genetic and parental FXI testing. Lifelong aspirin anticoagulation was commenced. No neurological sequelae were observed. UC remained uncontrolled despite escalation to non-conventional immunosuppression; he underwent subtotal colectomy and ileostomy formation with good results. Lowest FXI levels were documented pre-operatively (8 IU/dl) and treated with FXI concentrate. FXI showed spontaneous normalisation within 2 years of surgery (FXI >50 IU/dl) with no further complications. Conclusion: Clinicians should recognise the haematological implications of UC, especially in cases refractory to medical therapy. In our patient, FXI deficiency was secondary to uncontrollable gastrointestinal inflammation and normalised after definitive surgical treatment. P-086 Total colectomy in pediatric ulcerative colitis: a cohort study from a tertiary center in Greece K. Dimakou, S. Fessatou, M. Maragoudaki, I. Panayotou, E. Roma-Giannikou*, G. Chouliaras. University of Athens, “Aghia Sophia” Children’s Hospital, Athens, Greece Introduction: Pharmacological interventions may induce and maintain clinical remission in children with ulcerative colitis (UC). Nevertheless a significant proportion of these patients may, ultimately, undergo total colectomy (TC) due to refractory disease. Aim: To assess the observed rate of colectomy in pediatric UC in Greece. Patients were classified according to period of diagnosis (1981 1989, 199

P-084: Eosinophilic gastroenteropathy – from diet to colectomy

s of the 3rd International Symposium on Pediatric Inflammatory Bowel Disease S425 (perianal disease, ileal and/or colonic stenosis with or without abscess with respective resections). In multivariate analysis, no association with age, gender, inflammatory and serologic markers was detected. Conclusion: IBD is rare in our country, however extensive forms predominate and rates of surgery are high, especially in CD (40% by the final visit), with important resections, reflecting the severity of IBD. Family history and positivity of ANCA and ASCA are low; therefore, other factors have to be involved in this aggressive phenotype. P-084 Eosinophilic gastroenteropathy from diet to colectomy A. Karoliny1 *, J.B. Kovacs1, A. Nagy1, N. Lasztity1, K. Meichelbeck1, L. Karsza1, G. Hegyi1, B. Lestar2, G. Veres3, M. Lorincz1. 1Pal Heim Children’s Hospital, Budapest, Hungary, 2Hungarian Defence Forces Med. Center, Dept. of Surgery, Budapest, Hungary, 31st Dept. of Pediatrics, Semmelweis University, Budapest, Budapest, Hungary Background: Incidence of eosinophilic gastrointestinal disorders is increasing worldwide, however, precise pathomechanism, diagnostic criteria and therapy, in severe cases, is not well known. While there are well documented publications and guidelines about the clinical manifestation and treatment of eosinophilic oesophagitis the incidence, clinical manifestation and treatment of primary eosinophilic gastroenterocolitis is less well known. Case report: A 4-year-old male patient was presented with massive haematochesia and diarrhoea. Upper and lower endoscopy showed gastritis, duodenitis and ulcerative pancolitis, respectively. Histology confirmed eosinophilic gastroenteropathy (>100 eosinophils/HPF in duodenum and colon). 5-ASA, systemic corticosteroid, leukotriene receptor antagonist and azathioprine treatment combined with elimination diet did not achieve remission. Due to further deterioration and increased transfusion demand nasogastric feeding with amino acid based formula was introduced. After two years of unsuccessful conventional therapy infliximab was given, but due to severe bloody diarrhoea total colectomy was needed. After 12 months of follow-up, the patients is symptom free, growing well with no hematochezia. None of the upper and lower endoscopies, biopsies, and colectomy specimens showed any signs of Inflammatory Bowel Disease (IBD), but high number of eosinophils. Conclusion: IBD and eosinophilic colitis may share a common phenomenon resulting differencial diagnostic difficulties. Here, we presented a patient with severe form of eosinophilic gastroenteritis causes life-threatening hematochezia. Biological treatment and colectomy for this indication is rarely indicated. P-085 Resolution of factor XI (FXI) deficiency in refractory ulcerative colitis (UC) after surgery: a case report A. Angelakopoulou1 *, K. Lindley2, M. Mathias2, S. McCartney3, A. Windsor3, F. Kiparissi1. 1Great Ormond Street Hospital NHS Trust, London, United Kingdom, 2Great Ormond Street Hospital, London, United Kingdom, 3University College London Hospital NHS Trust, London, United Kingdom Introduction: Coagulation abnormalities in acute inflammatory conditions such as sepsis are well recognised. Chronic inflammatory conditions, such as Inflammatory Bowel Disease, have the potential to induce a coagulopathic or procoagulant state with potential devastating consequences. Aim: To report the resolution of severe FXI deficiency in severe UC post colectomy and restorative surgery. Methods: We present the case of a 14-year-old male with severe UC, FXI deficiency and an acute, thrombotic cerebrovascular event. Results: The patient presented with two-week history of headache, vomiting, altered consciousness and left leg weakness, one year after diagnosis of severe UC refractory to combination immunosuppressive therapy. MRI changes in keeping with Right Middle Cerebral Artery ischaemic stroke. Cerebral/visceral angiography excluded systemic vasculitis. Investigations revealed APTT of 67.3s (26 38s) and low FXI (13 IU/dl; 50 150). Platelet count showed a drop post stroke (lowest 76) with subsequent gradual recovery. Congenital FXI deficiency was excluded by patient genetic and parental FXI testing. Lifelong aspirin anticoagulation was commenced. No neurological sequelae were observed. UC remained uncontrolled despite escalation to non-conventional immunosuppression; he underwent subtotal colectomy and ileostomy formation with good results. Lowest FXI levels were documented pre-operatively (8 IU/dl) and treated with FXI concentrate. FXI showed spontaneous normalisation within 2 years of surgery (FXI >50 IU/dl) with no further complications. Conclusion: Clinicians should recognise the haematological implications of UC, especially in cases refractory to medical therapy. In our patient, FXI deficiency was secondary to uncontrollable gastrointestinal inflammation and normalised after definitive surgical treatment. P-086 Total colectomy in pediatric ulcerative colitis: a cohort study from a tertiary center in Greece K. Dimakou, S. Fessatou, M. Maragoudaki, I. Panayotou, E. Roma-Giannikou*, G. Chouliaras. University of Athens, “Aghia Sophia” Children’s Hospital, Athens, Greece Introduction: Pharmacological interventions may induce and maintain clinical remission in children with ulcerative colitis (UC). Nevertheless a significant proportion of these patients may, ultimately, undergo total colectomy (TC) due to refractory disease. Aim: To assess the observed rate of colectomy in pediatric UC in Greece. Patients were classified according to period of diagnosis (1981 1989, 199

Impact of automatic tube current modulation on CT dose and image quality from a view of nationwide survey

Impact of automatic tube current modulation on CT dose and image quality from a view of nationwide survey

Normocalcemic primary hyperparathyroidism in postmenopausal women: Relationship with bone metabolism

Normocalcemic primary hyperparathyroidism in postmenopausal women: Relationship with bone metabolism