MTUCH ATTENTION has been given to the development of a method for the determination of urinary glucose at clinically significant levels. A method, described by Froesch and Renold (1), determines glucose concentration by measuring the difference in reducing substances in the urine after incubation with glucose oxidase. A further development was the colorimetric quantitation of the hydrogen peroxide resulting from the oxidation of glucose in the presence of the enzyme. The glucose oxidase peroxidase systems, however, were primarily used for blood glucose because of substances in the urine which interfered with enzyme action. Huggett and Nixon (2) and Marks (3) have described methods adapted for use with urine which use activated charcoal to remove inhibitory susbtances, but this technique has the undesirable effect of removing some of the glucose as well. The combination of charcoal and Lloyd's reagent used by Beach and Turner (4) and by Kingsley and Getchell (5) is somewhat more effective. However, we agree with Salomon and Johnson (6) who have shown that this method is not completely satisfactory. They favored ion-exchange resins as a means of removing urinary inhibitors despite a somewhat cumbersome procedure requiring special apparatus. Jacobsen (7) tried yet another approach, attempting to minimize 'the problem of inhibitors by using a high dilution of urine. Consequently, this method is limited to specimens with larger amounts of glucose. All the methods described have some limitation. They are either affected by incomplete removal of enzyme inhibitors, too cumbersome, or unsuited for use with small amounts of glucose. Further, they do not have the advantage of speed or the potential for improved test reproducibility of an automated procedure. Hill and Kessler (8) succeeded in automating the glucose oxidase procedure, but they considered it suitable for blood glucose only. Our purpose, then, was to find a simple, rapid, and reliable quantitative method for urinary glucose which would be most sensitive at normal levels. Primary attention was given to the enzyme glucose oxidase as the specific means for measuring glucose (9, 10). Both the Hill-Kessler procedure and the preliminary preparation of urine as described by Salomon and Johnson (6) were modified and subsequently combined to give a rapid and accurate semiautomated method for urinary glucose. Our appraisal of this method included a recovery study, replicate determinations on samples from a pool of known glucose content performed over a period of time, and the introduction of blind duplicates. These methods of assessment were applied both to the semiautoMiss Kondon is a medical technologist and Dr. O'Sullivan is chief, Diabetes and Arthritis Field Research Section, Public Health Service, Boston, Mass.