Glass Microchannel Research Articles

Flow boiling characteristics of HFE-7000 in high aspect ratio microchannels with the effect of flow orientation

The aim of the present experimental study is to investigate the flow boiling characteristics in a high aspect ratio microchannel for two different channel orientations. Hydrofluoroether 7000 (HFE-7000) flowing within a rectangular glass microchannel (with a hydraulic diameter of 909 μm and a width-to-height aspect ratio of 10) coated with a tantalum layer to provide uniform heating along the channel was the configuration investigated. The data were recorded for mass flux of 14 − 42 kg m−2 s−1, while the heat flux was varied between 3.5 and 24.3kWm−2 under horizontal and vertical upward flow. Analysis of the experimental data reveals that thermal performance is influenced by both mass and heat fluxes, as well as flow orientation, and these are presented and discussed. The wall temperature mapping and heat transfer coefficient analysis showed that for low mass flow cases, the dry out occurs at a lower heat flux and for either flow orientation. At medium and higher heat fluxes, though, it is found that a horizontal flow produces dry out events more easily compared to vertical upward flow. The observed phenomenon during flow boiling also shows the importance of nucleate boiling and thin film evaporation on the heat transfer performance. In addition, pressure fluctuations during flow boiling were also recorded. A Fourier transform analysis of pressure fluctuations data showed that vertical upward flow produces a higher dominant frequency than horizontal flow. Finally, the analysis of thermal characteristics and pressure fluctuations can provide further insights into the design of devices where flow orientation plays a major role.

EchoGrid: High-Throughput Acoustic Trapping for Enrichment of Environmental Microplastics.

The health hazards of micro- and nanoplastic contaminants in drinking water has recently emerged as an area of concern to policy makers and industry. Plastic contaminants range in size from micro- (5 mm to 1 μm) to nanoplastics (<1 μm). Microfluidics provides many tools for particle manipulation at the microscale, particularly in diagnostics and biomedicine, but has in general a limited capacity to process large volumes. Drinking water and environmental samples with low-level contamination of microplastics require processing of deciliter to liter sample volumes to achieve statistically relevant particle counts. Here, we introduce the EchoGrid, an acoustofluidics device for high throughput continuous flow particle enrichment into a robust array of particle clusters. The EchoGrid takes advantage of highly efficient particle capture through the integration of a micropatterned transducer for surface displacement-based acoustic trapping in a glass and polymer microchannel. Silica seed particles were used as anchor particles to improve capture performance at low particle concentrations and high flow rates. The device was able to maintain the silica grids at a flow rate of 50 mL/min. In terms of enrichment, the device is able to double the final pellet's microplastic concentration every 78 s for 23 μm particles and every 51 s for 10 μm particles at a flow rate of 5 mL/min. In conclusion, we demonstrate the usefulness of the EchoGrid by capturing microplastics in challenging conditions, such as large sample volumes with low microparticle concentrations, without sacrificing the potential of integration with downstream analysis for environmental monitoring.

Universal selective transfer printing via micro-vacuum force

Transfer printing of inorganic thin-film semiconductors has attracted considerable attention to realize high-performance soft electronics on unusual substrates. However, conventional transfer technologies including elastomeric transfer printing, laser-assisted transfer, and electrostatic transfer still have challenging issues such as stamp reusability, additional adhesives, and device damage. Here, a micro-vacuum assisted selective transfer is reported to assemble micro-sized inorganic semiconductors onto unconventional substrates. 20 μm-sized micro-hole arrays are formed via laser-induced etching technology on a glass substrate. The vacuum controllable module, consisting of a laser-drilled glass and hard-polydimethylsiloxane micro-channels, enables selective modulation of micro-vacuum suction force on microchip arrays. Ultrahigh adhesion switchability of 3.364 × 106, accomplished by pressure control during the micro-vacuum transfer procedure, facilitates the pick-up and release of thin-film semiconductors without additional adhesives and chip damage. Heterogeneous integration of III-V materials and silicon is demonstrated by assembling microchips with diverse shapes and sizes from different mother wafers on the same plane. Multiple selective transfers are implemented by independent pressure control of two separate vacuum channels with a high transfer yield of 98.06%. Finally, flexible micro light-emitting diodes and transistors with uniform electrical/optical properties are fabricated via micro-vacuum assisted selective transfer.

Microfluidic Device Integrated With PDMS Microchannel and Unmodified ITO Glass Electrodes for Highly Sensitive, Specific, and Point-of-Care Detection of Copper and Mercury.

This work delves upon developing a two-layer plasma-bonded microfluidic device with a microchannel layer and electrodes for electroanalytical detection of heavy metal ions. The three-electrode system was realized on an ITO-glass slide by suitably etching the ITO layer with the help of CO2 laser. The microchannel layer was fabricated using a PDMS soft-lithography method wherein the mold created by maskless lithography. The optimized dimensions opted to develop a microfluidic device with length of 20 mm, width of 0.5 mm and gap of 1 mm. The device, with bare unmodified ITO electrodes, was tested to detect Cu and Hg by a portable potentiostat connected with a smartphone. The analytes were introduced in the microfluidic device with a peristaltic pump at an optimal flow rate of [Formula: see text]/min. The device exhibited sensitive electro-catalytic sensing of both the metals by achieving an oxidation peak at -0.4 V and 0.1 V for Cu and Hg respectively. Furthermore, square wave voltammetry (SWV) approach was used to analyze the scan rate effect and concentration effect. The device also used to simultaneously detect both the analytes. During simultaneous sensing of Hg and Cu, the linear range was observed between [Formula: see text] to [Formula: see text], the limit of detection (LOD) was found to be [Formula: see text] and [Formula: see text] for Cu and Hg respectively. Further, no interference with other co-existing metal ions was found manifesting the specificity of the device to Cu and Hg. Finally, the device was successfully tested with real samples like tap water, lake water, and serum with remarkable recovery percentages. Such portable devices pave way for detecting various heavy metal ions in a point-of-care environment. The developed device can also be used for detection of other heavy metals like cadmium, lead, zinc etc., by modifying the working electrode with the various nanocomposites.

XRD and Spectroscopic Investigations of ZIF-Microchannel Glass Plates Composites.

In this study, new composite materials comprising zeolitic imidazolate framework (ZIF) structures and microchannel glass (MCG) plates were fabricated using the hydrothermal method and their morphological and spectral properties were investigated using XRD, SEM, FTIR, and Raman spectroscopy. XRD studies of powder samples revealed the presence of an additional phase for a ZIF-8 sample, whereas ZIF-67 samples, which were prepared through two different chemical routes, showed no additional phases. A detailed analysis of the FTIR and micro-Raman spectra of the composite samples revealed the formation of stable ZIF structures inside the macropores of the MCG substrate. The hydrophilic nature of the MCG substrate and its interaction with the ZIF structure resulted in the formation of stable ZIF-MCG composites. We believe that these composite materials may find a wide range of important applications in the field of sensors, molecular sieving.

Experimental investigation of bubble dynamics and flow patterns during flow boiling in high aspect ratio microchannels with the effect of flow orientation

Multiphase flow and boiling phase-change within microchannels are of paramount importance to thermal management and electronics cooling, among others. The present experimental study investigated the bubble dynamics and flow patterns during flow boiling in a high aspect ratio microchannel at different channel orientations. Hydrofluoroether (HFE−7000) was used as the working fluid within a transparent glass microchannel with a width-to-height aspect ratio of 10 and a hydraulic diameter of 909 μm. A tantalum layer coating was applied to the channel, allowing visual observation to be conducted whilst imposing uniform heating along the channel. Under horizontal and vertical upward flow, the mass fluxes were varied between 14 kg m−2 s−1 and 42 kg m−2 s−1 for Reynolds numbers between 28.4 and 85.2, while the heat fluxes were set from 2.8 kW m−2 to 18.7 kW m−2. The results show that single bubble growth can be divided into three stages, namely: bubble-free growth, partially confined bubble growth, and fully confined growth. The effects of mass flux and heat flux on bubble behaviour and transition points between the defined stages are discussed. Equally important, the channel orientation influences the bubble behaviour in terms of bubble evolution, bubble shape, and bubble nose velocity, with no significant differences observed for the flow patterns when comparing horizontal and vertical upward cases. The comparison of the present work with previous flow pattern map results from the literature shows that earlier flow pattern transition models cannot accurately predict the observed flow patterns in the present configuration. Two types of instabilities are observed, which are associated with the dominant flow pattern during flow boiling and hence a function of the mass flux to heat flux ratio. The first type of instability, high amplitude and long period oscillation, is found during slug flow which is characterised by the presence of bubble rapid expansion along the channel. In addition, a second instability ensues when churn and annular flow create short period oscillations due to the high number of nucleation events, bubble coalescence, and the breakage of liquid slug or liquid film.

Canopy elastic turbulence: Spontaneous formation of waves in beds of slender microposts

A microfluidic canopy flow device, formed from a large array of slender polymeric pillars within a glass microchannel, is subjected to viscoelastic flow in the regime of elastic turbulence. The system results in the spontaneous emergence of waves in the form of propagating regions of low flow velocity compared to the bulk, also inducing Monami-like waves in the canopy if the pillars are flexible. Due to the analogies with classical (inertial) canopy turbulence, this new phenomenon is named ``canopy elastic turbulence''.

Micro-particle image velocimetry for blood flow in thick round glass micro-channels: Channel fabrication and velocity profile characterization

This method describes the use of thick round borosilicate glass micro-channels for blood flow visualization using micro-particle image velocimetry (µPIV) techniques. In contrast with popular methods using squared polydimethylsiloxane channels, this method allows for visualization of blood flow in channel geometries that resemble more the natural physiology of human blood vessels. With a custom designed enclosure, the microchannels were submerged in glycerol to reduce light refraction occurring during µPIV due to the thick walls of the glass channels. A method is proposed to correct the extracted velocity profiles from the µPIV to account for out-of-focus error. The customized elements of this method include:• The use of thick circular glass micro-channels,• a custom designed mounting solution for the channels on a glass slide for flow visualization,• a MATLAB code to correct velocity profile accounting for out-of-focus error.

Fabrication of Glass Microchannels Using Plant Roots and Nematodes

Microchannels have been studied actively in recent years. Various types of materials are used for fabricating microchannels in different types of applications including polymers, and ceramics. Since glass materials have appealing properties such as hardness, transparency, thermal resistance, and chemical resistance, they are used in biomedical devices and microfluidic application. Glass microchannel fabrication methods include chemical etching, imprinting, and 3D printing. This paper proposes unprecedented methods to fabricate glass microchannels using plant roots growth and nematodes migration. The microchannels fabricated by these methods have a complex three-dimensional structure, and the smallest channels have a diameter of several tens of micrometers in the glass. Although glass is attractive material, it is challenging to sinter the green body to fully dense and transparent solid structure. Hence, experiments were also conducted to establish a fabrication technique for transparent silica glass.

Stable and drag-reducing superhydrophobic silica glass microchannel prepared by femtosecond laser processing: Design, fabrication, and properties

The superhydrophobic silica glass microchannel with flow drag reduction performance has broad application prospects. In response to this demand, this paper proposes an integrated design and preparation method of structure and function for drag reduction microchannels on the silica glass surface. The design method of geometric parameters of stable superhydrophobic silica glass surface structures with a periodic micropillar array is studied based on the drag reduction mechanism of superhydrophobic surfaces. Combined with the plasma deposition process, the drag-reducing superhydrophobic silica glass surface is successfully prepared by femtosecond laser technology. The prepared drag-reducing superhydrophobic silica glass surface shows good composite wetting state stability under different test conditions. The drag-reducing superhydrophobic functional structure with a micropillar array is prepared at the bottom of the silica glass microchannels by femtosecond laser direct writing technology. Moreover, the effective integration of the silica glass microchannel structure and the drag-reducing functional structure is achieved. The preparation and flow performance test of microfluidic devices demonstrates that drag-reduction microchannels of silica glass can significantly reduce microfluidic flow resistance. The proposed method effectively improves the functional characteristics of ultrafast laser processing surface microchannels in silica glass and has broad application prospects in fluid flow and control.

Manufacture of Three-Dimensional Optofluidic Spot-Size Converters in Fused Silica Using Hybrid Laser Microfabrication

We propose a hybrid laser microfabrication approach for the manufacture of three-dimensional (3D) optofluidic spot-size converters in fused silica glass by a combination of femtosecond (fs) laser microfabrication and carbon dioxide laser irradiation. Spatially shaped fs laser-assisted chemical etching was first performed to form 3D hollow microchannels in glass, which were composed of embedded straight channels, tapered channels, and vertical channels connected to the glass surface. Then, carbon dioxide laser-induced thermal reflow was carried out for the internal polishing of the whole microchannels and sealing parts of the vertical channels. Finally, 3D optofluidic spot-size converters (SSC) were formed by filling a liquid-core waveguide solution into laser-polished microchannels. With a fabricated SSC structure, the mode spot size of the optofluidic waveguide was expanded from ~8 μm to ~23 μm with a conversion efficiency of ~84.1%. Further measurement of the waveguide-to-waveguide coupling devices in the glass showed that the total insertion loss of two symmetric SSC structures through two ~50 μm-diameter coupling ports was ~6.73 dB at 1310 nm, which was only about half that of non-SSC structures with diameters of ~9 μm at the same coupling distance. The proposed approach holds great potential for developing novel 3D fluid-based photonic devices for mode conversion, optical manipulation, and lab-on-a-chip sensing.

Oil Displacement in Calcite-Coated Microfluidic Chips via Waterflooding at Elevated Temperatures and Long Times.

In microfluidic studies of improved oil recovery, mostly pore networks with uniform depth and surface chemistry are used. To better mimic the multiple porosity length scales and surface heterogeneity of carbonate reservoirs, we coated a 2.5D glass microchannel with calcite particles. After aging with formation water and crude oil (CRO), high-salinity Water (HSW) was flooded at varying temperatures and durations. Time-resolved microscopy revealed the CRO displacements. Precise quantification of residual oil presented some challenges due to calcite-induced optical heterogeneity and brine–oil coexistence at (sub)micron length scales. Both issues were addressed using pixel-wise intensity calibration. During waterflooding, most of the ultimately produced oil gets liberated within the first pore volume (similar to glass micromodels). Increasing temperature from 22 °C to 60 °C and 90 °C produced some more oil. Waterflooding initiated directly at 90 °C produced significantly more oil than at 22 °C. Continuing HSW exposure at 90 °C for 8 days does not release additional oil; although, a spectacular growth of aqueous droplets is observed. The effect of calcite particles on CRO retention is weak on flat surfaces, where the coverage is ~20%. The calcite-rich pore edges retain significantly more oil suggesting that, in our micromodel wall roughness is a stronger determinant for oil retention than surface chemistry.

Shape regulation of tapered microchannels in silica glass ablated by femtosecond laser with theoretical modeling and machine learning

Shape regulation of tapered microchannels in silica glass ablated by femtosecond laser with theoretical modeling and machine learning

Highly efficient photocatalytic oxidation of C[sbnd]H bond based on microchannel reactor

A mild photocatalytic CH bond selective oxidation mediated by metal-free photocatalyst 2-Methylanthraquinone was reported. Glass microchannel reactor can enhance the absorption of photon energy and oxygen by the reaction mixture, so as to greatly improve the reaction efficiency of photocatalytic reaction in glass microchannel reactor. This reaction method can quickly oxidize alkyl aromatics into corresponding oxidation products to obtain aromatic ketones in high yield.

JMEMS Letters Fabrication of Self-Sealed Circular Microfluidic Channels in Glass by Thermal Blowing Method

We report fabrication of circular glass microchannels with radius less than 100 <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu \text{m}$ </tex-math></inline-formula> using the glass blowing technique. Silicon trenches filled with nitrogen are used for microchannel fabrication. We report an interesting reflow regime where the flowing glass fills the silicon trenches to form a completely circular microchannel. Circular microchannels have symmetric velocity profile and do not suffer from flow stagnation problems observed in rectangular channels. Besides circular shape, fabrication conditions of the semi-circular and elliptically shaped channels are also discussed. A mathematical model explaining the observation is also presented and is compared with the experimental results. [2021-0183]

Dissolution-After-Precipitation (DAP): a simple microfluidic approach for studying carbonate rock dissolution and multiphase reactive transport mechanisms.

We propose a simple microfluidic approach: Dissolution-After-Precipitation (DAP), to investigate regimes of carbonate rock dissolution and multiphase reactive transport. In this method, a carbonate porous medium is created in a glass microchannel via calcium carbonate precipitation, after which an acid is injected into the channel to dissolve the precipitated porous medium. Utilizing the DAP method, for the first time we realized all five classical single-phase carbonate rock dissolution regimes (uniform, compact, conical, wormhole, ramified wormholes) in a microfluidic chip. The results are validated against the established theoretical dissolution diagram, which shows good agreement. Detailed analysis of these single-phase dissolutions suggests that the heterogeneity of the porous medium may significantly impact how the dissolution patterns evolve over time. Furthermore, DAP is utilized to investigate multiphase dissolution. As examples we tested the cases of an oleic phase (tetradecane) and a gaseous phase (CO2). Results show that the presence of a nonaqueous phase in pore spaces induces the formation of wormholes despite weak advection, and these wormholes ultimately become pathways for nonaqueous phase transport. However, the transport of tetradecane in the wormhole is very slow, causing acid breakthrough into neighboring regions. This mechanism enhances lateral connectivity between wormholes and may lead to a wormhole network. In contrast, CO2 moves rapidly and continuously seeks to enter a widening wormhole from a narrower wormhole or the porous regions, generating phenomena such as ganglia redistribution and counterflow (flow of gas opposite to acid flow). Extensive independent experiments are conducted to verify the reproducibility of the observed phenomena/mechanisms and further analyze them. Real-time monitoring of fluid pressure drop during dissolution is implemented to complement microscopy image analysis. Our method can be implemented repeatedly on the same chip, which offers a convenient and inexpensive option to study pore-scale reactive transport mechanisms.

Glass Microchannel Formation by Mycelium

We propose a new method to fabricate complicated 3-dimensional glass microchannels. We employed mycelium for this purpose. Mycelium possesses a complicated, fine and three-dimensional network structure. We cultivated mycelium in silica compounds, and subsequently silica compounds were heated to be sintered. During this heating process, all the mycelium was burned off and remained a fine network channel structure in a transparent glass chip. We also tried to control of the growth of this mycelium. The growth could be changed by growth conditions. In this work, we used cyclic mechanical stimuli for this purpose. We set cyclic tensile strain to the sample under growing mycelium. This cyclic strain caused anisotropic growth of the mycelium in some condition.

Development of an Optical Method for the Evaluation of Whole Blood Coagulation.

Blood coagulation is a defense mechanism, which is activated in case of blood loss, due to vessel damage, or other injury. Pathological cases arise from malfunctions of the blood coagulation mechanism, and rapid growth of clots results in partially or even fully blocked blood vessel. The aim of this work is to characterize blood coagulation, by analyzing the time-dependent structural properties of whole blood, using an inexpensive design and robust processing approaches. The methods used in this work include brightfield microscopy and image processing techniques, applied on finger-prick blood samples. The blood samples were produced and directly utilized in custom-made glass microchannels. Color images were captured via a microscopy-camera setup for a period of 35 min, utilizing three different magnifications. Statistical information was extracted directly from the color components and the binary conversions of the images. The main advantage in the current work lies on a Boolean classification approach utilized on the binary data, which enabled to identify the interchange between specific structural elements of blood, namely the red blood cells, the plasma and the clotted regions, as a result of the clotting process. Coagulation indices produced included a bulk coagulation index, a plasma-reduction based index and a clot formation index. The results produced with the inexpensive design and the low computational complexity in the current approach, show good agreement with the literature, and a great potential for a robust characterization of blood coagulation.

Functionalized microchannels as xylem-mimicking environment: Quantifying X. fastidiosa cell adhesion

Microchannels can be used to simulate xylem vessels and investigate phytopathogen colonization under controlled conditions. In this work, we explore surface functionalization strategies for polydimethylsiloxane and glass microchannels to study microenvironment colonization by Xylella fastidiosa subsp. pauca cells. We closely monitored cell initial adhesion, growth, and motility inside microfluidic channels as a function of chemical environments that mimic those found in xylem vessels. Carboxymethylcellulose (CMC), a synthetic cellulose, and an adhesin that is overexpressed during early stages of X. fastidiosa biofilm formation, XadA1 protein, were immobilized on the device’s internal surfaces. This latter protocol increased bacterial density as compared with CMC. We quantitatively evaluated the different X. fastidiosa attachment affinities to each type of microchannel surface using a mathematical model and experimental observations acquired under constant flow of culture medium. We thus estimate that bacterial cells present ∼4 and 82% better adhesion rates in CMC- and XadA1-functionalized channels, respectively. Furthermore, variable flow experiments show that bacterial adhesion forces against shear stresses approximately doubled in value for the XadA1-functionalized microchannel as compared with the polydimethylsiloxane and glass pristine channels. These results show the viability of functionalized microchannels to mimic xylem vessels and corroborate the important role of chemical environments, and particularly XadA1 adhesin, for early stages of X. fastidiosa biofilm formation, as well as adhesivity modulation along the pathogen life cycle.

Experimental Analysis of Laser Micromachining of Microchannels in Common Microfluidic Substrates.

Laser micromachining technique offers a promising alternative method for rapid production of microfluidic devices. However, the effect of process parameters on the channel geometry and quality of channels on common microfluidic substrates has not been fully understood yet. In this research, we studied the effect of laser system parameters on the microchannel characteristics of Polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA), and microscope glass substrate—three most widely used materials for microchannels. We also conducted a cell adhesion experiment using normal human dermal fibroblasts on laser-machined microchannels on these substrates. A commercial CO2 laser system consisting of a 45W laser tube, circulating water loop within the laser tube and air cooling of the substrate was used for machining microchannels in PDMS, PMMA and glass. Four laser system parameters—speed, power, focal distance, and number of passes were varied to fabricate straight microchannels. The channel characteristics such as depth, width, and shape were measured using a scanning electron microscope (SEM) and a 3D profilometer. The results show that higher speed produces lower depth while higher laser power produces deeper channels regardless of the substrate materials. Unfocused laser machining produces wider but shallower channels. For the same speed and power, PDMS channels were the widest while PMMA channels were the deepest. Results also showed that the profiles of microchannels can be controlled by increasing the number of passes. With an increased number of passes, both glass and PDMS produced uniform, wider, and more circular channels; in contrast, PMMA channels were sharper at the bottom and skewed. In rapid cell adhesion experiments, PDMS and glass microchannels performed better than PMMA microchannels. This study can serve as a quick reference in material-specific laser-based microchannel fabrications.