Geographic Representativeness Research Articles

A New Geographic Transfer Method of Wave Data

Savasta, D.; Nucera, A.; Barbaro, G., and Foti, G., 2022. A new geographic transfer method of wave data. Journal of Coastal Research, 38(3), 557–566. Coconut Creek (Florida), ISSN 0749-0208. The availability of quality, site-specific wave data is necessary for an accurate understanding of wave climate. Direct and indirect wave data often have insufficient spatial resolution to ensure the requirement for geographic representativeness of data. The proposed wave data transfer method, in two versions, transfers an entire time series of wave data from a point with data to a neighboring point. The method is based on the empirical relationships of the Shore Protection Manual method and involves estimating appropriate statistical parameters from wind data characterizing the area comprising the two points. The method has been tested and compared with De Girolamo and Contini's transfer method in three applications in the Mediterranean Sea, showing significantly better results in two applications and similar results in a third application. It is believed that the lower reliability of the new transfer method in one of the three applications results from the considerable distance, 112 km, between the data point and the transfer point. Transfer methods are more accurate in cases of modest distances between the two points, which is the classic application of the transfer method in engineering practice.

Evaluation of trace-element contamination from serum collection tubes used by the California Biobank Program

BackgroundSerum samples archived by the California Biobank Program (CBP) can be a valuable resource to researchers with multiple benefits: affordability, relatively large sample sizes, and racial and geographical representativeness. However, there has been little attention given to the reliability of CBP samples for trace-element analysis. The objectives of this study are to estimate the contamination levels from the serum separation tubes (SST, BD 367983) utilized by the CBP for 13 trace elements (Cr, Mn, Co, As, Se, Sr, Mo, Cd, Sb, Hg, Tl, Pb and U) and to evaluate the feasibility of the use of CBP serum samples for biomonitoring trace elements in human body. MethodsSerum separation tubes were tested using deionized (DI) water and whole blood and compared with two alternative sampling devices, plasma separation tubes (PST, BD 365047) and acid-cleaned blood tubes (ABT, BD 367856). Results and conclusionsThe leaching tests for SSTs with DI water demonstrated that detectable levels of Cr, Mn, Co, Sr, Sb, Pb and U were measured, while Sb was elevated. Tests of PSTs also revealed contamination of Mn, Co, Sr and Sb, with Co and Sr being much higher than those found from SSTs. As a more direct approach to estimate trace element contamination, a 45-day time series was conducted using human blood. The differences in elemental concentrations leached into serum/plasma was not considerable between the three types of sampling tubes for Cr, As, Se, Mo, Cd, Hg and Tl. However, SSTs had far greater concentrations than the ABTs for Mn, Co, Sr, Sb and U. For Co and Sr, the PSTs had higher concentrations throughout the experiment than both ABTs and SSTs. Pb showed lower concentrations from the PSTs than the other tubes; we speculate this may be due to re-suspension of settled cellular materials that are elevated in Pb, or re-dissolution of Pb from these materials. Trace-element measurements from 200 samples archived by the CBP using SSTs suggest that SST contamination was negligible for Se and Mo. For Mn, As, Sr, Cd and Hg, based on our leaching results, only a fraction of these samples had considerably high concentrations (e.g., > 10×) compared to the contamination from the SST. For Cr, Co, Sb, Tl, Pb and U, analyte levels were too low in most samples compared to the contamination from the SSTs. Our study also demonstrated that the PSTs could be a “cleaner” alternative to SSTs for analytes such as Cr, As, Cd, Hg, Tl, Pb and U.

A comparison of coronavirus disease 2019 and seasonal influenza surveillance in five European countries: France, Germany, Italy, Spain and the United Kingdom.

BackgroundIn response to the coronavirus disease (COVID‐19) outbreak that unfolded across Europe in 2020, the World Health Organisation (WHO) called for repurposing existing influenza surveillance systems to monitor COVID‐19. This analysis aimed to compare descriptively the extent to which influenza surveillance systems were adapted and enhanced and how COVID‐19 surveillance could ultimately benefit or disrupt routine influenza surveillance.MethodsWe used a previously developed framework in France, Germany, Italy, Spain and the United Kingdom to describe COVID‐19 surveillance and its impact on influenza surveillance. The framework divides surveillance systems into seven subsystems and 20 comparable outcomes of interest and uses five evaluation criteria based on WHO guidance. Information on influenza and COVID‐19 surveillance systems were collected from publicly available resources shared by European and national public health agencies.ResultsOverall, non‐medically attended, virological, primary care and mortality surveillance were adapted in most countries to monitor COVID‐19, although community, outbreak and hospital surveillance were reinforced in all countries. Data granularity improved, with more detailed demographic and medical information recorded. A shift to systematic notification for cases and deaths enhanced both geographic and population representativeness, although the sampling strategy benefited from the roll out of widespread molecular testing. Data communication was greatly enhanced, contributing to improved public awareness.ConclusionsWell‐established influenza surveillance systems are a key component of pandemic preparedness, and their upgrade allowed European countries to respond to the COVID‐19 pandemic. However, uncertainties remain on how both influenza and COVID‐19 surveillance can be jointly and durably implemented.

PAGE Study: Summary of a Study Protocol to Estimate the Prevalence of Severe Asthma in Spain Using Big Data Methods

Background: The proposal and the initiative for the Prevalence of Severe Asthma in Hospital Units in Spain (PAGE) study came about because of the widespread implementation of electronic medical records and the limited data available on the prevalence of severe asthma in hospitals in our setting. Objectives: The primary objective was to determine the prevalence of severe asthma in the outpatient clinics of allergy and pulmonology departments in Spain. The secondary objectives were to describe the most prevalent characteristics and phenotypes of severe asthma, to evaluate the selection criteria for receiving approved biological treatments for this disease, and to estimate consumption of resources. Furthermore, digital technology and new data collection sources made it possible to reuse information stored in electronic medical records (Big Data). The study was performed using one such tool, Savana. The PAGE study was a multicenter, nonexperimental, observational, cross-sectional study in the first phase and a prospective study in the second phase. The study was controlled and population-based, with 2-stage selection of patients by random sampling. The research was carried out in 40 hospitals selected using convenience sampling in order to ensure geographical representativeness in Spain. This manuscript describes the study design and protocol. Our study design was sufficiently robust to avoid bias and to establish the prevalence of patients with severe asthma in Spanish hospitals. It was also the first to incorporate new tools that can help in routine clinical practice and research, such as big data analysis software, and to evaluate the reliability and efficiency of these tools.

Is geographic sampling bias representative of environmental space?

Species occurrence data from public repositories are widely used in biogeography, and conservation research. However, these data are prone to several sampling biases limiting their usefulness in biodiversity studies, and particularly in species distribution models (SDMs). Specifically, a geographic sampling bias can lead to overfitted SDMs while an environmental sampling bias can affect the estimate of the true environmental space occupied by a species. Several methods can be used to correct for sampling biases, but most of these are focused on the geographic component only. We aim to assess if sampling bias correction based on geographical factors can represent a viable solution also for environmental bias. We focused our analyses on plants and invertebrates in Africa. To model sampling bias in the spatial dimension we estimated sampling rates as a function of distance from a set of potential bias factors (roads, cities, waterbodies, and coastline) in a Bayesian framework. To model sampling bias in the environmental dimension we calculated the environmental multivariate distance between the average climate in the species' occurrences and the rest of the study area by using a set of bioclimatic variables. We quantified the spatial relationship between geographic and environmental bias calculating a Local Indicator of Multivariate Spatial Association (LISA), highlighting local clusters of spatial correlation. For both plants and invertebrates, almost 50% of Africa showed a non-significant relationship between the geographical and the environmental bias components, indicating the absence of a direct correspondence between the two types of biases. Furthermore, from 20.4% to 24.4% of the study area showed an opposite pattern between the two biases, clearly indicating that a correction in the spatial component would be detrimental in the environmental component. Our findings showed that geographical factors cannot be considered as good proxies for environmental bias and, consequently, we suggest considering both geographic and environmental corrections when sampling bias is a problem.

Global effects of land-use intensity on local pollinator biodiversity

Pollinating species are in decline globally, with land use an important driver. However, most of the evidence on which these claims are made is patchy, based on studies with low taxonomic and geographic representativeness. Here, we model the effect of land-use type and intensity on global pollinator biodiversity, using a local-scale database covering 303 studies, 12,170 sites, and 4502 pollinating species. Relative to a primary vegetation baseline, we show that low levels of intensity can have beneficial effects on pollinator biodiversity. Within most anthropogenic land-use types however, increasing intensity is associated with significant reductions, particularly in urban (43% richness and 62% abundance reduction compared to the least intensive urban sites), and pasture (75% abundance reduction) areas. We further show that on cropland, the strongly negative response to intensity is restricted to tropical areas, and that the direction and magnitude of response differs among taxonomic groups. Our findings confirm widespread effects of land-use intensity on pollinators, most significantly in the tropics, where land use is predicted to change rapidly.

Ramsar Wetlands of International Importance–Improving Conservation Outcomes

The Ramsar Convention (or the Convention on Wetlands), signed in 1971, was one of the first international conservation agreements, promoting global wise use of wetlands. It has three primary objectives: national designation and management of wetlands of international importance; general wise use of wetlands; and international cooperation. We examined lessons learnt for improving wetland conservation after Ramsar’s nearly five decades of operation. The number of wetlands in the Ramsar Site Network has grown over time (2,391 Ramsar Sites, 2.5 million km2, as at 2020-06-09) but unevenly around the world, with decreasing rate of growth in recent decades. Ramsar Sites are concentrated in countries with a high Gross Domestic Product and human pressure (e.g., western Europe) but, in contrast, Ramsar Sites with the largest wetland extent are in central-west Africa and South America. We identified three key challenges for improving effectiveness of the Ramsar Site Network: increasing number of sites and wetland area, improved representation (functional, geographical and biological); and effective management and reporting. Increasing the number of sites and area in the Ramsar network could benefit from targets, implemented at national scales. Knowledge of representativeness is inadequate, requiring analyses of functional ecotypes, geographical and biological representativeness. Finally, most countries have inadequate management planning and reporting on the ecological character of their Ramsar Sites, requiring more focused attention on a vision and objectives, with regular reporting of key indicators to guide management. There are increasing opportunities to rigorously track ecological character, utilizing new tools and available indicators (e.g., remote sensing). It is critical that the world protect its wetlands, with an effective Ramsar Convention or the Convention on Wetlands at the core.

Data-Quality Assessment Signals Toxic-Site Safety Threats and Environmental Injustices.

Most hazardous-waste sites are located in urban areas populated by disproportionate numbers of children, minorities, and poor people who, as a result, face more severe pollution threats and environmental-health inequalities. Partly to address this harm, in 2017 the United Nations unanimously endorsed the New Urban Agenda, which includes redeveloping urban-infill-toxic-waste sites. However, no systematic, independent analyses assess the public-health adequacy of such hazardous-facility redevelopments. Our objective is to provide a preliminary data-quality assessment (PDQA) of urban-infill-toxic-site testing, conducted by private redevelopers, including whether it adequately addresses pollution threats. To this end, we used two qualitative, weight-of-evidence methods. Method 1 employs nine criteria to select assessments for PDQA and help control for confounders. To conduct PDQA, Method 2 uses three US Environmental Protection Agency standards—the temporal, geographical, and technological representativeness of sampling. Our Method 1 results reveal four current toxic-site assessments (by CBRE/Trammell Crow, the world’s largest commercial developer); at all of these sites the main risk drivers are solvents, volatile organic compounds, including trichloroethylene. Our Method 2 results indicate that all four assessments violate most PDQA standards and systematically underestimate health risk. These results reveal environmental injustice, disproportionate health threats to children/minorities/poor people at all four sites. Although preliminary, our conclusion is that alleviating harm and environmental-health inequalities posed by urban-infill-toxic-site pollution may require improving both the testing/cleanup/redevelopment requirements of the New Urban Agenda and the regulatory oversight of assessment and remediation performed by private redevelopers.

Rapid review of five years of Aboriginal and Torres Strait Islander health research in Australia – persisting under‐representation of urban populations

ObjectiveTo review how published Aboriginal and Torres Strait Islander health research reflects the geographical distribution of the Indigenous population of Australia. MethodsRapid review using Lowitja Institute Lit.search tool for PubMed indexed Indigenous health research papers (January 2013 to January 2018). Geographic location, participant age, study type and recruitment site were identified for each paper. ResultsA total of 1,258 research papers were identified: 190 (15%) focused exclusively on Indigenous people living in urban areas; 563 (45%) in rural/remote areas; and 505 (40%) spanned urban and rural/remote areas. Despite similar burdens of disease, three times as many papers were published per 1,000 DALYs for rural/remote areas than urban areas. ConclusionsIndigenous health research publications have more than doubled since 2010. However, research focusing on the health needs of urban Indigenous people remains low relative to disease burden and population. Implications for public healthMore research to address the health needs of Indigenous people living in urban areas is required although this should not be at the expense of research for rural and remote areas. Increased funding quarantined for Indigenous health research, coupled with self‐determination of the research agenda and reporting on the geographic representativeness of research, may help address geographical inequities in research outputs.

ИССЛЕДОВАНИЕ СЕЗОННОЙ ДИНАМИКИ ЛАНДШАФТОВ УРАЛА В ПАРАДИГМЕ ФУНКЦИОНАЛЬНО-ДИНАМИЧЕСКОГО ПОДХОДА: ИСТОРИЯ И СОВРЕМЕННОСТЬ

The article deals with the results of phenological studies of the Urals carried out under the functional-dynamic approach to the consideration of the region’s spatial-temporal structure. The authors analyze the history of phenological research in the Urals conducted under the charge of V.A. Batmanov. The article considers the phenological network in the historical aspect from the late 19th century to the modern period. The authors analyzed the schemes of physicogeographical zoning of the Urals and established the hierarchical level of natural complexes that reflect the territory’s specificity and serve as a basis for geographical referencing of the regional phenological network. The study provides results of systematization and digital processing of multi-year archival data of the Russian Geographical Society, fund materials and expeditionary research in the 20th–21st centuries. Structural and spatial analysis of the phenological network conducted for the landscape provinces of the Northern, Middle and Southern Urals allowed us to identify its specificity, which is expressed in ramification, but also unevenness and inconsistency with the landscape diversity of the region. Quantitative analysis and modelling of the phenological network make it possible to develop recommendations for its expansion, including through optimization by a more complete inclusion of the environmental fund. The completeness and geographical representativeness of the phenological network will allow optimizing the monitoring of the landscapes and climate dynamics, which is important for the development of fundamental science and for applied problems related to the long-term sustainable development of the region.

Recruitment strategies and geographic representativeness for patient survey studies in rare diseases: Experience from the living with myeloproliferative neoplasms patient survey.

BackgroundRecruitment of individuals with rare diseases for studies of real-world patient-reported outcomes is limited by small base populations. Myeloproliferative neoplasms (MPNs) are a group of rare, chronic, hematologic malignancies. In this study, recruitment strategies and geographic representativeness from the Living with MPNs survey are reported.MethodsThe Living with MPNs online cross-sectional survey was conducted between April and November 2016. Individuals 18 to 70 years of age living in the United States and diagnosed with an MPN were eligible to participate. Recruitment approaches included direct contact via emails and postcards; posts on MPN-focused social media and patient advocacy websites; postcard mailings to doctors’ offices; and advertisements on medical websites, Google, and Facebook. Geographic representativeness was assessed based on the number of survey respondents living in each state or the District of Columbia and by the number of survey respondents per 10 million residents.ResultsA total of 904 respondents with MPNs completed the survey. The recruitment method yielding the greatest number of respondents was advertisements on MPN-focused social media (47.6% of respondents), followed by emails (35.1%) and postcards (13.9%) sent through MPN advocacy groups. Home state information was provided by 775 respondents from 46 states (range of respondents per state, 1–89). The number of respondents per 10 million residents in the 46 states with respondents ranged from 12.1 to 52.7.ConclusionsRecruitment using social media and communications through patient groups and advocacy organizations are effective in obtaining geographically representative samples of individuals with MPNs in the United States. These approaches may also be effective in other rare diseases.

PIN146 Factors Associated with HPV Vaccination of Girls Aged 11-18 YEARS Old in Greece

To investigate which factors are associated with parents’ decision to vaccinate their daughters aged 11-18 years old against the Human Papilloma Virus (HPV). The study was conducted with structured interviews. The study sample was random, national, stratified by geographic region and representative of the general population of parents with girls aged 11-18 years old. The study collected data on knowledge, attitudes and practices regarding HPV and HPV vaccine. A binary logistic regression model was constructed to assess which factors are associated with the vaccination of girls against HPV. The final study sample consisted of 1,000 participants, the vast majority of which was female (99.0%) and mean age 45.56 years old (SD=5.005). Overall, 47.0% of the parents stated that their daughters had been vaccinated against HPV, but further data on doses of vaccination received revealed that only 35.0% of the total sample had completed the vaccination scheme (2 doses for girls <15 years old & 3 doses for girls ≥15 years old). Regarding the regression model, the following variables were found to have a statistically significant association with HPV vaccination: perception on how easily HPV is transmitted (OR=1.105), level of trust in the medical body for prevention information provision (OR=1.205), overall perception on the significance of children/adolescents’ vaccination (OR=0.618), parent age (OR=1.125), internet/social media as the main source of information on HPV (OR=0.886) and treating physician’s suggestion to vaccinate daughter against HPV (OR=7.319). No statistically significant association was found for receiving information on HPV by the treating physician and avocation with religion. The results of the study highlight the important role of healthcare professionals in affecting parents’ decision to vaccinate their daughters against HPV, while the internet/social media seem to have a negative effect on vaccination.

Feasibility and Quality of a National RDD Smartphone Web Survey: Comparison With a Cell Phone CATI Survey

Internet-enabled smartphones and wireless communication technologies are opening new ways to conduct web-based self-administered data collection for academic or nonacademic research. Considering the relative advantages of self-administration such as the low cost, overall convenience, and collection of better data about sensitive topics, survey researchers are eager to explore conducting national web surveys of the general population via smartphones, especially if they can use probability-based random-digit-dialing (RDD) sampling methods. But questions about the feasibility of such surveys remain. We conducted an experiment using national samples drawn from an RDD wireless sampling frame to compare two administration methods: a smartphone web survey using SMS (text messages) invitations and a cell phone (smartphone or feature phone) survey through computer-assisted telephone interviewing (CATI). This study was conducted using the National Survey of Smoking and Health in South Korea, a country with a very high rate of smartphone ownership. The geographic and demographic representativeness of respondents in the smartphone web mode (self-administered mode) was similar to that of the cell phone CATI survey (interviewer-administered mode), although the completion rate in the former was nearly half that of the latter. A majority of demographic variables and measures of experiences, attitudes, and perceptions of cigarettes or smoking showed significant mode differences for both unweighted and weighted estimates. The total cost of the web survey was about one fifth that of the telephone survey. The results demonstrate the potential of a smartphone web survey as a stand-alone or primary mode of data collection, if carefully designed and implemented.

Geoinformation Modeling of Determination a Soil Pollution by Lead Compounds in Highway Agroecosystems

This work examines the method of geoinformation modeling in determining the degree of soil pollution by Pb compounds in the highway ecosystems, used as agricultural areas. The methodology allows identifying the spatial parameters of the area of pollution and the territories safe for cultivating crops by determining the actual level of soil contamination. The actual level of pollution (ALP) reflects the estimated balance of Pb compounds in soils over a specified period of time and the duration of pollution (or decontamination) period under the conditions of existing anthropogenic pressure. ALP is defined as the difference between the calculated amount of Pb compounds that have been introduced into the soils of the geosystems from the main sources of emissions and their calculated amount that has been lost from the soil and/or removed from migratory flows (by being transferred to the state conditionally inaccessible by plants), for the same time interval. Methodology verification took place in the geographic area representative of forest-steppe zone of Ukraine by its environmental conditions and agricultural use. Methodology implementation was conducted by building spatial regional databases, utilizing the data from field and laboratory soils studies and open sources of digital spatial data. Based on geoinformation modeling, we determined the configuration and quantitative characteristics of the primary zone of contamination of the soil surface layer with Pb compounds. We presented the calculation procedure of quantitative indicators of soil decontamination from Pb compounds as they redistribute through the soil profile via main geochemical scenarios. We also provided the rate of this process under the conditions of the existing anthropogenic pressure. Using modeling results, we mapped areas of removal, transit and accumulation of Pb compounds and territories receiving pollution from highway geosystems. Verification of the modeling results during laboratory studies proved the objectivity of the proposed methodology in determining the current levels of pollution with Pb compounds. Our methodology could be utilized for a set of different environmental factors as well as other heavy metals with a purpose of identifying areas safe for cultivating crops.

Future Directions for Dementia Risk Reduction and Prevention Research: An International Research Network on Dementia Prevention Consensus.

In the past decade a large body of evidence has accumulated on risk factors for dementia, primarily from Europe and North America. Drawing on recent integrative reviews and a consensus workshop, the International Research Network on Dementia Prevention developed a consensus statement on priorities for future research. Significant gaps in geographical location, representativeness, diversity, duration, mechanisms, and research on combinations of risk factors were identified. Future research to inform dementia risk reduction should fill gaps in the evidence base, take a life-course, multi-domain approach, and inform population health approaches that improve the brain-health of whole communities.

Evaluating 5.5 Years of Equinella: A Veterinary-Based Voluntary Infectious Disease Surveillance System of Equines in Switzerland.

Equine health is important in regard to trade, economy, society, and the veterinary, as well as public health. To reduce the burden of equine infectious diseases internationally, it is important to collect, review, and distribute equine health surveillance data as accurate and timely as possible. Within this study, we aimed at providing a comprehensive descriptive analysis of data submitted to Equinella, a voluntary veterinary-based surveillance system of non-notifiable equine infectious diseases and clinical signs, in Switzerland. This was achieved by reviewing the reports submitted since its relaunch in November 2013 and until April 2019, as well as assessing the data validity, activeness of participating veterinarians, coverage of the equine population, geographical representativeness, and timeliness of the system. In total, 630 reports have been submitted. Data validity ranged between 88.2 and 100%. The coverage of Equinella was assessed to be 50.8% of the Swiss equine population. Over the 5.5 years, of all 102 registered veterinarians, 67 (65.7%) submitted at least one report. On average, these veterinarians submitted 1.7 reports per year (median = 4 reports). More recently, in 2018, approximately only one-third [29 (28.4%)] of all registered veterinarians submitted at least one report. However, 59 (57.8%) have responded to the monthly reminder emails to confirm that they have not observed any relevant clinical case to be reported at least once (median number of confirmation per veterinarian = 9 of 12 reminder emails). The incidence of reports varied between cantons (member states of the Swiss confederation). The median timeliness of report submission was found to be 7 days. Overall, Equinella has been receiving reports since its initiation and contributed continuously to the surveillance of infectious diseases in the Swiss equine population and provided an output for the international equine community. Challenges encountered in achieving a higher number of submitted reports and increasing the coverage of the equine population, as well as the overall activeness of veterinarians, require further work. With our study, we provide a comprehensive overview of a veterinary-based voluntary surveillance system for equine health, assessed challenges of such, and suggest concrete improvements with transdisciplinary approaches for similar veterinary-based surveillance systems.

Investigating Foreign Direct Investment Attractive Factors of Korean Direct Investment into Vietnam

This paper aims to investigate FDI attractive factors, which are important to formulate policies to attract Korean direct investment into Vietnam. Based on the literature review and the results of interview with 27 Korean investors in Vietnam, we determined the model of variables attracting Korea’s FDI into Vietnam. It is used to assess the impact of attractive factors belonging to three groups of variables to support investment decision; they are macroeconomics variables (including market size factor, labor cost factor, and market openness factor), policies variables (including monetary policy factor and tax rate gap factor), and microeconomics variables (geographic advantage factor representative by location). This research also utilized a relatively new quantitative research method based on the Autoregressive Distributed Lag model (ARDL) with the time data chain from 1995 to 2017 of Korean FDI into Vietnam. It analyzes long-term relationships between dependent variables and independent variables. The result of this study indicates that there are three positive factors (low wages, trade openness and government policy) explaining the FDI flows in the long term. The result also shows that incentive tax policy has had a positive impact on Korean FDI, which has satisfied the aim of seeking efficiency of Korean investors.

Conserving orthodox seeds of globally threatened plants ex situ in the Millennium Seed Bank, Royal Botanic Gardens, Kew, UK: the status of seed collections

We reviewed the status of orthodox seed collections of globally threatened plants conserved in − 20 °C long-term storage at the Millennium Seed Bank, Royal Botanic Gardens, Kew, UK in terms of their geographic and bioclimatic representativeness, taxonomic and genetic diversity, quality and physiological status. The comprehensive dataset used spans over 45 years of worldwide conservation effort across various organisations. The data provides evidence-based results and future directions for the represented globally threatened flora that are of relevance to all plant conservation and seed banking organisations across the globe. The reviewed sample includes 523 collections and represents a wide geographic range, originating from 67 countries, from all nine bio-geographic continents. The majority of collections originated from temperate climates and from habitats with no dry seasons but experiencing warm summer periods. The taxonomic composition of the collections highlighted a substantial diversity, with 303 taxa (four extinct in the wild; 56 critically endangered; 105 endangered; and 138 vulnerable) represented by 297 species, 199 genera and 74 families. Almost four fifths of the collections were harvested from wild habitats. Whilst wild-origin collections can harbour useful genes not available in the cultivated gene pool, for threatened plants both collections and taxa are likely to suffer from low genetic diversity as a low number of individual plants, populations and/or potentially viable or usable seeds were sampled at the original harvest. Large numbers of empty and infested seeds in the original harvest have significantly affected the quality of collections in terms of availability of potentially viable or usable seeds in collections. As a result, just over one third of taxa and one fifth of collections consisted of ≥ 5000 potentially viable or usable seeds. Viable seeds exhibited a sound physiological status in terms of germinability and viability at the initial round of germination tests after storage, but on average, relative germination and viability achieved were below 85%. A decline in germinability during their variable time of storage was evident for 16% of the 78 collections analysed for longevity. According to a set of criteria, suitable germination protocols for propagation of plants from seeds were identified for 165 taxa. Given the apparent differences between wild species, especially those that are rare and threatened, and domesticated crops, the quality and physiological status of reviewed collections are reasonably sound. The characteristics we observed for collections, the challenges we identified for conserving them and the germination protocols we suggested for propagation of plants from seeds have the scope to be noted, integrated and used globally across various conservation activities and policies.

Evaluation of global leaf area index and fraction of absorbed photosynthetically active radiation products over North America using Copernicus Ground Based Observations for Validation data

With a growing number of Earth observation (EO) products available through operational programmes such as the European Union’s Copernicus, there is increasing emphasis on product accuracy and uncertainty, necessitating evaluation against in situ reference measurements. Whilst existing reference datasets have proven a valuable resource, they incorporate little data with which products from recent EO instruments can be assessed. A reliance on individual field campaigns has also led to several inconsistencies, whilst limiting the extent to which temporal variations in EO product performance can be captured. Recently established environmental monitoring networks such as the National Ecological Observatory Network (NEON), which collect routine in situ measurements using standardised instruments and protocols, provide a promising opportunity in this respect. The Copernicus Ground Based Observations for Validation (GBOV) service was initiated in recognition of this fact. In the first component of the project, raw observations from existing networks have been collected and processed to provide reference data for a range of EO land products. In this study, we focus on leaf area index (LAI) and the fraction of absorbed photosynthetically active radiation (FAPAR). Raw digital hemispherical photography (DHP) from twenty NEON sites was processed to derive in situ reference measurements, which were then upscaled to provide high spatial resolution reference maps. Using these data, we assess the recently released Copernicus Global Land Service (CGLS) 300 m Version 1 (V1) products derived from PROBA-V, in addition to existing products derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) and Visible Infrared Radiometer Suite (VIIRS). When evaluated against reference data, the CGLS 300 m V1 product demonstrated the best agreement (RMSD = 0.57 for LAI and 0.08 for FAPAR), followed by the Collection 6 VNP15A2H and MOD15A2H products (RMSD = 0.81 to 0.89 for LAI and 0.12 for FAPAR). Differing assumptions of the products and in situ reference measurements, which cause them to be sensitive to slightly different quantities, are thought to explain apparent biases over sparse vegetation and forest environments. To ensure their continued utility, future work should focus on updating the GBOV in situ reference measurements, implementing additional corrections, and improving their geographical representativeness.

Dynamic probabilistic material flow analysis of engineered nanomaterials in European waste treatment systems

Knowledge on the material flows of engineered nanomaterials (ENMs) is crucial for assessing their environmental risks. Waste management processes constitute important parts of material flow analyses as they affect large fractions of the ENMs. Accordingly, their detailed representation could substantially improve the models. Our goal was to consider the temporal variations of wastewater and solid waste management in the dynamic probabilistic material flow analysis of carbon nanotubes (CNTs), nano-Ag, -TiO2 and -ZnO in Europe from 2000 to 2020. New input parameters included wastewater and solid waste management rates for each year. The uncertainties associated with these data were assessed based on the type of consulted source, the geographical representativeness and temporal concordance. Results show modal values of 10–27% of ENMs going from sorting to reprocessing. Large shares of environmental releases of nano-Ag and nano-ZnO end in surface water (4.9 t and 1700 t respectively in 2020), while sludge-treated soil as environmental compartment is receiving most of nano-TiO2 (22,000 t in 2020) and CNTs (8.8 t in 2020). Discharges from wastewater management to the subsurface soil make this compartment the largest environmental sink of nano-Ag and nano-ZnO (30 t and 3860 t accumulated in 2020, respectively). Landfills represent significant stocks of ENMs, with 105 t, 2077 t, 69,000 t and 1042 t of nano-Ag, nano-ZnO, nano-TiO2 and CNTs. This model includes detailed descriptions of waste management and sources of ENMs released at the European scale. However, a better understanding of the behaviour, i.e. fate and potential transformations of ENMs in reprocessing systems, is needed to complete the full assessment.