Hybrid Generation Research Articles

The Competitive Strategy of Small Operators of Food Business

This research investigates how small operators adapt to the environment that is changing in a brittle way. The objectives of the study are to: 1) identify the companies’ competitive strategies 2) evaluate the demographics characteristics of the companies whose performance level is above average and 3) Identify the changing pattern of the successful strategy of small operators. The research employs quantitative techniques. A questionnaire, pretested for its reliability and content validity, was sent to 383 business operators in the city municipality of Petchburi. There were 383 responses for a response rate of 100%. The statistical methods used to analyze the includes frequency, percentage, means, standard deviation, and clustering. The findings indicate that most vendors prepare made-to-order items. The small operators of food business the most importance on innovation strategy,The findings show that above average performers focus on innovations and hybrid generation group. Innovation principles should be utilized.

Self-powered wireless sensor node enabled by ultra-high-output swinging hybrid generator toward real-time and in-situ marine meteorological observations

With the intensification of global ocean exploration, there is an increasing need for extensive and dispersed wireless sensor networks (WSNs) to meet the requirements of real-time, in-situ, and high-spatial-temporal-density marine meteorological observations. Nevertheless, providing a reliable power source and ensuring the ongoing maintenance of these WSNs pose significant challenges. Here, an innovative self-powered wireless sensor node is developed to address these challenges, featuring an ultra-high-output triboelectric-electromagnetic hybrid generator (T-EHG) with a swinging mechanism. The T-EHG, which combines triboelectric (TENG) and electromagnetic (EMG) generation, assisted by a universal power management system (UPMS), efficiently converts wave energy into a consistent voltage output between 1.5 V to 3.3 V. Additionally, the self-powered WSNs integrated with T-EHG, UPMS circuits, meteorological sensors, and microcontroller units (MCUs) with LoRa wireless transmission functionality are capable of executing data collection and transmission operations every 10 s under simulated wave conditions. These systems can achieve a maximum wireless transmission range of 1.5 km. Notably, a marine meteorological monitoring system utilizes these autonomous sensor nodes, along with base stations, cloud servers, and terminal devices to effectively implement real-time, on-site measurements of temperature, humidity, and air pressure within the monitored zone. This research underscores the substantial practical utility and extensive application prospects of these advanced self-powered WSNs for marine meteorological surveillance.

Synthesis of C3'-Foused Aryl/Penta-1,4-Dien-3-One/Amine Hybrids as HSP90C-Terminal Inhibitors.

24 C3'-focused hybrids of aryl/penta-1,4-dien-3-one/amine (APDA) were designed and synthesized. Of these hybrids, 2 n demonstrated improved antiproliferative effects on HER2-positive breast cancer cells (SKBr3 and BT474) and triple-negative breast cancer (TNBC) cells (MDA-MB-231 and MDA-MB-468) with IC50 values ranging from 7.45 to 10.75 μM, but less toxicity to normal breast cells MCF-10A than the first generation of hybrid 1. Additionally, 2 n retained its ability to inhibit HSP90C-terminus, leading to the degradation of HSP90 client proteins HER2, EGFR, pAKT, AKT, and CDK4, without inducing a heat-shock response. Notably, 2 n also demonstrated improved thermostability compared to 1 and maintained in vitro metabolic stability in simulated intestinal fluid. These findings will provide a scientific basis for developing HSP90C-terminal inhibitors in the future.

СТРЕССОУСТОЙЧИВЫЙ, С ХОРОШИМ КОМПЛЕКСОМ ОСНОВНЫХ ХОЗЯЙСТВЕННЫХ ПРИЗНАКОВ СОРТ ПШЕНИЦЫ ОЗИМОЙ ТЕРСИНКА

A new variety of soft winter wheat, Tersinka, has been developed in the North Caucasus Federal Scientific Agrarian Center of the Stavropol Region. The objective of research is to create adaptive, stress-resistant genotypes of universal wheat with a set of economic traits for cultivation using intensive and medium-intensive technologies in the conditions of the North Caucasus, Lower Volga and Central Black Earth regions of Russia. New varieties are bred using modern methods of classical, haploid and marker selection. It is based on intraspecific, complex, stepwise hybridization and, starting from the third generation of hybrids (F3), continuous, targeted, individual selection of new genotypes is carried out based on the traits of the model of universal wheat varieties of varying intensity that we have developed. The experiment was carried out on fallow, the method of variety placement was systematic. In the last crossing, the Don 95 variety was used as the maternal form, the Verta variety served as the paternal form. The Tersinka variety belongs to the Erythrospermum variety. During the years of study (2021–2023), the new variety always reliably exceeded the Grom standard in grain yield. On average, this excess over the standard was 1.67 t/ha. According to the growing season, Tersinka belongs to mid-season varieties. The new variety has a higher productive tillering. It is distinguished by high drought and heat resistance and reliably exceeds the standard in terms of resistance to these stresses. According to flour-baking indicators, it belongs to strong wheat improvers. It is distinguished by high field resistance to biotic stressors and a complex of major diseases.

Research on Perfume User Experience Evaluation Based on Human Machine Hybrid Generation Adversarial Network

Research on Perfume User Experience Evaluation Based on Human Machine Hybrid Generation Adversarial Network

A wideband vibration sensor with piecewise nonlinear and up-frequency coupling strategy for mechanical fault monitoring

Vibration analysis using vibration sensors is an effective method of monitoring mechanical faults. However, most current vibration sensors have a narrow working frequency range and a low-frequency vibration response. Therefore, broadening the frequency band to detect high-frequency vibration signals is imperative. This paper proposes a self-powered vibration sensor based on a broadband hybrid generator (VS-BHG) that incorporates a piecewise nonlinear and up-frequency coupling strategy through collisions between the suspension and fixed part, thereby effectively expanding the operational frequency range. By implementing this strategic approach, the VS-BHG can effectively capture the vibration energy across a broad frequency spectrum ranging from 5 to 38 Hz and exhibit a robust linear response to acceleration within the frequency range of 20–1600 Hz. A machine running state monitoring system based on the VS-BHG is established, enabling the recognition of 16 different running states and fault types in industrial fans with an accuracy rate of 98.38 % using a deep-learning model. This paper presents an efficient and viable method for monitoring broadband mechanical fault.

Expression Patterns of TGF-β1, TβR-I, TβR-II, and Smad2 Reveal Insights into Heterosis for Growth of Hybrid Offspring between Acanthopagrus schlegelii and Pagrus major.

TGF-β1/Smads is a classic signaling pathway, which plays important roles in the development process of organisms. Black porgy Acanthopagrus schlegelii and red porgy Pagrus major are valuable economic fishes, and their hybrid offspring show excellent heterosis traits. Yet the molecular regulation mechanism of the heterosis traits is less clear. Here, we explored the TGF-β1/Smads pathway's molecular genetic information for heterosis in A. schlegelii ♂ × P. major ♀ (AP) and A. schlegelii ♀ × P. major ♂ (PA) in terms of growth and development. The mRNA expression levels of TGF-β1, TβR-I, TβR-II, and Smad2 genes in different developmental stages of A. schlegelii were detected. Furthermore, the expression levels of TGF-β1, TβR-I, TβR-II, and Smad2 genes in different tissues of adult (mRNA level) and larva (mRNA and protein level) of A. schlegelii, P. major, and their hybrids were determined by both real-time quantitative PCR and Western blot techniques. The results indicated the ubiquitous expression of these genes in all developmental stages of A. schlegelii and in all tested tissues of A. schlegelii, P. major, and its hybrids. Among them, the mRNA of TGF-β1, TβR-I, and TβR-II genes is highly expressed in the liver, gill, kidney, and muscle of black porgy, red porgy, and their hybrid offspring. There are significant changes in gene and protein expression levels in hybrid offspring, which indirectly reflect hybrid advantage. In addition, there was no correlation between protein and mRNA expression levels of Smad2 protein. The results provide novel data for the differential expression of growth and development genes between the reciprocal hybridization generation of black porgy and red porgy and its parents, which is conducive to further explaining the molecular regulation mechanism of heterosis in the growth and development of hybrid porgy.

Steady Output Triboelectric-Electromagnetic Hybrid Generator with Variable Drag Turbine Blades for Natural Wind Energy Harvesting.

In recent years, the triboelectric-electromagnetic hybrid generator (TEHG) has been widely studied. However, the problems of unsteady output and high starting wind speed of traditional TEHG in the wind energy environment have not been effectively solved. This work introduces an innovative solution in the form of a steady output triboelectric-electromagnetic hybrid generator (SO-TEHG) with variable drag turbine blades. The SO-TEHG integrates the energy management circuit to output steady electric energy under random wind conditions. In addition, the integration of variable drag turbine blades with the triboelectric nanogenerator (TENG) reduces the wind speed threshold required for SO-TEHG activation. In comparison to the traditional turbine blades, which necessitate a minimum wind speed of 3 m/s, the SO-TEHG's innovative design allows it to commence power generation at a lower 2 m/s wind speed, producing an additional output of 50 V. This enhanced starting capability in mild breezes positions the SO-TEHG as an ideal power source for applications. In practical farmland settings, experimental results conclusively demonstrate the SO-TEHG's ability to successfully activate soil hygrothermographs and hydrogen sensors. As a steady power source driven by gentle winds, the SO-TEHG holds tremendous promise for advancing smart agriculture.

The Complex Genetic Legacy of Hybridization and Introgression between the Rare Ocotea loxensis van der Werff and the Widespread O. infrafoveolata van der Werff (Lauraceae).

Hybridization and introgression are complex evolutionary mechanisms that can increase species diversity and lead to speciation, but may also lead to species extinction. In this study, we tested the presence and genetic consequences of hybridization between the rare and Ecuadorian endemic O. loxensis van der Werff and the widespread O. infrafoveolata van der Werff (Lauraceae). Phenotypically, some trees are difficult to identify, and we expect that some might in fact be cryptic hybrids. Thus, we developed nuclear microsatellites to assess the existence of hybrids, as well as the patterns of genetic diversity and population structure in allopatric and sympatric populations. The results revealed high levels of genetic diversity, even in the rare O. loxensis, being usually significantly higher in sympatric than in allopatric populations. The Bayesian assignment of individuals into different genetic classes revealed a complex scenario with different hybrid generations occurring in all sympatric populations, but also in allopatric ones. The absence of some backcrossed hybrids suggests the existence of asymmetric gene flow, and that some hybrids might be more fitted than others might. The existence of current and past interspecific gene flow also explains the blurring of species boundaries in these species and could be linked to the high rates of species found in Ocotea.

Magnetotropic Hybrid Generator with Self‐Switching Mechanism for Long‐Term Forest Fire Prevention

Magnetotropic Hybrid Generator with Self‐Switching Mechanism for Long‐Term Forest Fire Prevention

The Genetic Characterization of the Canarian Endemic Palm (Phoenix canariensis) by Simple Sequence Repeats and Chloroplast Markers: A Tool for the Molecular Traceability of Phoenix Hybridization

The endemic palm from the Canary Islands, Phoenix canariensis, is one of the most distinctive elements of the Canarian vegetation landscape, contributing to cultural, economic and environmental aspects. One of the main conservation problems facing this iconic palm is anthropogenic hybridization with other Phoenix species, particularly Phoenix dactylifera, which has been introduced extensively throughout its geographical range. Therefore, it is important to obtain a genetic tool that addresses different issues that may have an impact on the protection of P. canariensis, including ornamental applications and wild population conservation purposes. Our main goals were to detect a molecular tracer that could reliably distinguish between Phoenix canariensis and P. dactylifera in the Canary archipelago and to characterize the presence and extent of genetic hybridization events between the two species. We used 19 nuclear microsatellites and 1 chloroplast minisatellite set and analysed a large sample size (N = 433) of plants using both Bayesian methods and ordination techniques. Our data showed that a set of 13 nuclear markers revealed diagnostic alleles for P. canariensis, which were defined as the Canarian nuclear genotype (CNG). Moreover, P. canariensis exhibited an exclusive chlorotype of 266 bp that together with the GNC serve as an indicator of genetic purity in the Canarian palm. These markers are sufficient to detect any hybrid, even if it is not related to morphological differences. The occurrence of a considerable number of specimens with different degrees of hybridization is discussed in terms of the existence of different generations of hybrids and different types of crosses. Thus, the genetic tracers represent an invaluable tool to address any proposal for the genetic conservation of Phoenix canariensis.

Hybridization and introgression in deeply differentiated salamander species – molecular genetics and a reappraisal of Dr. Louis Vallée’s osteological data

Abstract Two morphologically and genetically distinct salamander species (the crested newt, Triturus cristatus and the marbled newt, T. marmoratus) engage over a large area in the west of France where they hybridize at ca. 4%. The species interaction is characterized by ecological differentiation and limited gene flow beyond the F1 hybrid generation. Incompletely isolated species like these allow to investigate the genetic mechanisms and evolutionary forces that maintain their identity in the face of ongoing gene flow, to which a large mosaic hybrid zone provides excellent opportunities. A reanalysis of published morphological data supports the partial breakdown of the species barrier whereas extensive genetic data show that introgression is low yet asymmetric, in line with the dynamics of species replacement inferred earlier. The current work, with seven diagnostic nuclear markers studied for a large sample, revises the estimates of introgression in both T. cristatus (to 0.24%) and T. marmoratus (to 0.11%). Difficulties remain in the recognition of potential triploid hybrids versus backcross hybrids. Haldane’s rule is partially supported, but deeper analyses require the use of a molecular marker for sex that is not yet available.

Development of a novel hybrid hydrogen generator incorporated into a combined system for buildings in sustainable communities

The development of a new hybrid hydrogen generator, using both photoelectrochemical process and conventional electrolysis, integrated into a combination system for buildings is a major step forward in the quest for sustainable energy solutions in cities where it is specifically designed, implemented and evaluated for incorporation into sustainable community systems. A significant advantage of this hydrogen generator is the ability to produce hydrogen constantly, regardless of the absence of solar irradiation. Furthermore, both electrolyzers contribute to a rise in the amount of hydrogen produced. Furthermore, the utilization of PEC electrolysis for hydrogen generation results in a significant reduction in the overall power requirements of the system. This new study investigates an integrated power system that produces electricity from solar energy and tackles issues related to freshwater supply, hydrogen production, and heating/cooling demands. The purpose of this research is to integrate advanced system components, including a solar power tower system, a combined Brayton-Rankine cycle, a multi-effect desalination (MED) unit, a new hybridized hydrogen generator system, an absorption cooling cycle (ACS), and a hydrogen storage and refuelling station. The overall energy efficiency is determined to be 49%, while the exergy efficiency becomes 46.2%.

Historical and contemporary processes driving the origin and structure of an admixed population within a contact zone between subspecies of a north temperate diadromous fish.

Hybridization between divergent lineages can result in losses of distinct evolutionary taxa. Alternatively, hybridization can lead to increased genetic variability that may fuel local adaptation and the generation of novel traits and/or taxa. Here, we examined single-nucleotide polymorphisms generated using genotyping-by-sequencing in a population of Dolly Varden char (Pisces: Salmonidae) that is highly admixed within a contact zone between two subspecies (Salvelinus malma malma, Northern Dolly Varden [NDV] and S. m. lordi, Southern Dolly Varden [SDV]) in southwestern Alaska to assess the spatial distribution of hybrids and to test hypotheses on the origin of the admixed population. Ancestry analysis revealed that this admixed population is composed of advanced generation hybrids between NDV and SDV or advanced backcrosses to SDV; no F1 hybrids were detected. Coalescent-based demographic modelling supported the origin of this population about 55,000 years ago by secondary contact between NDV and SDV with low levels of contemporary gene flow. Ancestry in NDV and SDV varies within the watershed and ancestry in NDV was positively associated with distance upstream from the sea, contingent on habitat-type sampled, and negatively associated with the number of migrations that individual fish made to the sea. Our results suggest that divergence between subspecies over hundreds of thousands of years may not be associated with significant reproductive isolation, but that elevated diversity owing to hybridization may have contributed to adaptive divergence in habitat use and life history.

Highly Sensitive Hybrid Triboelectric Nanogenerator with Ferris‐Wheel‐Like Structure for Ocean Wave Energy Harvesting

AbstractOcean wave energy represents a widely distributed and abundant clean, renewable energy source. However, its efficient harnessing remains a challenge. In this study, a triboelectric‐electromagnetic hybrid generator of a Ferris‐wheel‐like structure (FWS‐TEHG) with magnetic repulsion assistance is proposed to effectively enhance the collection of low‐frequency and low‐amplitude water wave energy. The Ferris‐wheel shell and the internal rotator are designed with a phase difference to heighten the swing amplitude, while the introduction of magnetic repulsion augments the motion frequency. The device has demonstrated excellent performance in low‐frequency conditions, from laboratory to ocean wave tests. Operating at a frequency of 0.5 Hz and a swing angle of 12° on a six‐freedom platform, it lights up 64 LEDs with a power rating of 2 W. Triggered by simulated water waves with a frequency of 1 Hz, the FWS‐TEHG charges a 19 mF capacitor at an average charging rate of ≈0.58 W h−1, powering a water‐level alarm. In oceanic conditions, the FWS‐TEHG effectively harvests energy from water waves by exhibiting an output frequency approximately four to five times higher than that of the primary frequency of ocean waves, thus enabling it to power electrical devices such as temperature–humidity meters efficiently. This study provides a valuable reference for advancing the practical application of nanogenerators in natural ocean environments.

Specific anti-SVCV antibodies in hybrids of common carp (Cyprinus carpio) and gibel carp (Carassius gibelio) reflect heterosis advantage and genetic breakdown

Spring viraemia of carp virus (SVCV) is a disease with a serious economic impact on the farming of cyprinid fish. High susceptibility to SVCV has been documented mostly in common carp (Cyprinus carpio), whilst gibel carp (Carassius gibelio) is a weakly susceptible species. Specific anti-SVCV antibodies were analyzed in pure lines of common carp and gibel carp, and various generations of F1 and post-F1 hybrids. Specifically, we focused on anti-SVCV antibodies in weakly susceptible F1 hybrids and highly infected backcross and F2 hybrids, hypothesizing that the level of anti-SVCV antibodies will reflect heterosis advantage in F1 generations and hybrid breakdown in post-F1 generations.Backcross generations with common carp exhibited the most frequently symptomatic infections, followed by pure common carp. The high capacity of susceptible common carp to produce anti-SVCV antibodies was reported, whilst gibel carp expressed almost undetectable levels of anti-SVCV antibodies. We found the very good capacity of F1 hybrids to produce anti-SVCV antibodies, suggesting heterosis advantage. In contrast, we revealed different patterns of anti-SVCV antibodies production between maternal backcrosses (having parents with the same mtDNA – here, gibel carp) and paternal backcrosses (having parents with different mtDNA, i.e., resulting from reciprocal crosses of F1 hybrids with gibel carp mtDNA and pure common carp). Whilst both maternal backcross generations and the F2 generation of hybrids tended to produce low anti-SVCV antibodies, both paternal backcross generations expressed levels of anti SVCV-antibodies similar to those of highly susceptible common carp. The low capacity to produce anti-SVCV antibodies in F2 hybrids may be interpreted as a potential consequence of genetic disruption. However, the different capacity to induce an adaptive response in paternal hybrids and maternal hybrids (with mtDNA C. gibelio) may be interpreted as potential compensation for the cyto-nuclear breakdown hypothesized for paternal hybrids when coping with viral infection. Alternatively, the genetic background of hybrid fish, i.e. a combination of the genes of highly susceptible common carp and of less susceptible gibel carp in the hybrid genome may be responsible for this pattern.

Schistosoma haematobium and Schistosoma bovis first generation hybrids undergo gene expressions changes consistent with species compatibility and heterosis.

When two species hybridize, the two parental genomes are brought together and some alleles might interact for the first time. To date, the extent of the transcriptomic changes in first hybrid generations, along with their functional outcome constitute an important knowledge gap, especially in parasite species. Here we explored the molecular and functional outcomes of hybridization in first-generation hybrids between the blood fluke parasites Schistosoma haematobium and S. bovis. Through a transcriptomic approach, we measured gene expression in both parental species and hybrids. We described and quantified expression profiles encountered in hybrids along with the main biological processes impacted. Up to 7,100 genes fell into a particular hybrid expression profile (intermediate between the parental expression levels, over-expressed, under-expressed, or expressed like one of the parental lines). Most of these genes were different depending on the direction of the parental cross (S. bovis mother and S. haematobium father or the reverse) and depending on the sex. For a given sex and cross direction, the vast majority of genes were hence unassigned to a hybrid expression profile: either they were differentially expressed genes but not typical of any hybrid expression profiles or they were not differentially expressed neither between hybrids and parental lines nor between parental lines. The most prevalent profile of gene expression in hybrids was the intermediate one (24% of investigated genes). These results suggest that transcriptomic compatibility between S. haematobium and S. bovis remains quite high. We also found support for an over-dominance model (over- and under-expressed genes in hybrids compared to parental lines) potentially associated with heterosis. In females in particular, processes such as reproductive processes, metabolism and cell interactions as well as signaling pathways were indeed affected. Our study hence provides new insight on the biology of Schistosoma hybrids with evidences supporting compatibility and heterosis.

Cytogenetic analysis of meiotic behaviour and stability in a trigeneric hybrid (triticale x trigopiro).

Trigeneric hybrids in Triticeae may help to establish evolutionary relationships among different genomes present in the same cellular genetic background and to transfer different alien characters into cultivated wheat. In the present study, a trigeneric hybrid involving species of Triticum, Secale, and Thinopyrum was synthesized by crossing hexaploid triticale with hexaploid trigopiro. The meiotic behaviour of chromosomes belonging to different genomes was analyzed, using routine and in situ hybridization techniques in F1, F2, and F3 generations of the trigeneric hybrid. The purpose of this study was to determine the chromosome number and genomic constitution and to discuss the mechanisms involved in the stabilization of the artificial tricepiro hybrids. The chromosome number of the trigeneric F1 hybrid was 2n = 42. Between 12 and 16 bivalents were observed in the central zone of the equatorial meiotic plate and between 9 and 18 univalents were found in the periphery of the MI equatorial plate. Seven of these univalents showed hybridization signals with rye DNA. Lagging rye and non-rye chromosomes and separation of sister chromatids were found in anaphase I. Tetrads with a maximum of six micronuclei, with and without hybridization signals of rye DNA, were observed. After three generations, meiotic cells revealed the presence of 42 chromosomes and 21 bivalents in diakinesis cells. The presence of 14 rye (Secale cereale) chromosomes and the complete pairing of chromosomes in F3 hybrids suggest that rye chromosomes would be preferentially transmitted to the progeny and that an elimination mechanism would act on chromosomes of Thinopyrum and wheat D genome.

A highly efficient self-powered variable impendence system

As an efficient mechanical energy harvester, the triboelectric-electromagnetic hybrid generator (TEHG) stands as a cornerstone in self-powered systems. Nevertheless, significant impedance disparities between triboelectric nanogenerators (TENGs) and electromagnetic generators (EMGs) often hamper systems’ energy utilization efficiency, attributed to impedance mismatch at the load. Here, a variable impedance strategy is proposed, aimed at maximizing the utilization of mechanical energies converted by TEHG. This approach capitalizes on electronic components with dynamic impedance from GΩ to kΩ in response to OFF-ON state transitions, thus matching the impedance of TENG and EMG. Experimentally, an ultraviolent gas discharge tube (UV-GDT) is integrated into the self-powered variable impedance system. Operated at 240 rpm, the TEHG-driven UV-GDT extracts energy amounting to 1304.27 mJ with an 87.5 % utilization efficiency. These metrics outperform the situation where UV-GDT is individually powered by either EMG (0 mJ, 0 %) or TENG (18.24 mJ, 60.7 %). Furthermore, the mechanical energy-activated UV system demonstrates promise for sterilization, curing, and photo-chemical reactions. This variable impedance strategy resolves the impendence mismatch between TEHG and load, more importantly, provides a valuable guideline for developing hybrid generator systems with enhanced energy utilization efficiency.

Наследяване на стопански ценни признаци и хетерозис при хибридни комбинации зимен ечемик

The article presents the results of the analysis of the inheritance of the main economic valuable traits of barley hybrids of the first generation using indicators of the degree of phenotypic dominance and heterosis. 6 hybrid barley combinations were evaluated according to the following characteristics: plant height (cm), productive twining, length of main spike (cm), number of grains in main spike, grain weight per spike (g), grain weight per plant (g), mass of 1000 grains (g). The research was carried out at the Institute of Agriculture-Karnobat in 2022-2023. Highly productive varieties and samples of barley, Bulgarian selection and from introduction were used as parental forms. For each investigated trait, the arithmetic mean ( x ), the error of the arithmetic mean (S %), variation coefficient (VC,%), the degree of dominance (d/a), true (HP) and hypothetical (MP) heterosis were determined. Based on the variation and inheritance in the early F1 hybrid generations, conclusions were drawn regarding the value of the cultivars used and the type of inheritance of the traits analyzed.