Based on WGCNA and machine learning studies, SMURF2 drives NSCLC malignant transformation, ferroptosis, and macrophage polarization by ubiquitinating SPP1.

Appropriate threshold setting and multiple methods combination may improve reproducibility of gene ontology enrichment analysis.

Rising global temperatures and growing demand for sustainable protein are concurrent challenges driving the expansion of aquaculture, including farming of shellfish such as the Australian Redclaw Crayfish (Cherax quadricarinatus). Although environmental stressors alter animal physiology, their effects on food protein allergenicity remain unexplored, particularly in shellfish, a leading cause of food-induced anaphylaxis. This study investigated how rearing temperature affects protein and allergen expression in redclaw reared at either 17°C, 28°C, or 32°C. Raw and cooked protein extracts were analysed using SDS-PAGE and immunoblotting with crustacean-allergic individuals (n=19). Whole extracts were examined by liquid-chromatography mass spectrometry (LC-MS) to identify and quantify proteins, assess differential expression, and perform gene ontology (GO) analysis across temperatures. Significant temperature-dependent variation in protein and allergen profiles was observed. IgE-binding intensity increased at 17°C and decreased at 32°C in raw and cooked extracts, indicating increased allergenicity under cold-stress. At 17°C, allergens including heat shock protein 70 (HSP70) and fatty acid-binding protein (FABP) were upregulated, alongside GO categories related to protein folding and metabolic/catabolic processes (e.g., triosephosphate isomerase (TPI), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), enolase, and fructose-bisphosphate aldolase (FBA)). GO expression profiles between 28°C and 32°C were largely similar. Allergens tropomyosin (TM), myosin light chain (MLC), and troponin C (TnC) were unchanged across temperatures. Environmental temperature alters the allergen/protein profiles of redclaw and clinically relevant IgE-antibody reactivity. These findings highlight the impact of environmental and farming conditions when assessing food allergen exposure risks in a changing climate and highlight the need for further validation in other aquaculture species.

DisSubFormer: A subgraph transformer model for disease subgraph representation and comorbidity prediction.

Exercise rejuvenates bone marrow mesenchymal stem cells associated with the inhibition of inflammatory factors and senescence-related factors.

Glaucocalyxin D (GLD), an ent-kaurane diterpenoid isolated from Isodon species, exhibits extensive pharmacological potential; however, its mechanism of action against acute myeloid leukemia (AML) remains to be elucidated. The present study employed a combined network pharmacology and experimental approach to elucidate the anti-AML mechanisms of GLD. Potential targets were identified using database mining and a protein-protein interaction network was constructed. The Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analyses highlighted the JAK-STAT signaling pathway as central to action by GLD. Molecular docking predicted stable binding of GLD to core targets, including STAT3. Experimental validation in HEL and K562 AML cells demonstrated that GLD potently and dose-dependently inhibits cell proliferation, with efficacy similar to the standard chemotherapeutic agent doxorubicin. Mechanistically, GLD suppressed the phosphorylation of JAK2 and STAT3. GLD also induced mitochondrial apoptosis by modulating the Bcl-2/Bax ratio and triggered G2/M phase arrest by downregulating cyclin B1 and CDK1. These findings delineated a coherent mechanism whereby GLD exerts anti-leukemic effects by inhibiting the JAK-STAT pathway, supporting its potential as a novel lead compound for AML therapy in the future.

Transcriptomic profiling reveals T cell-mediated Glutamine immunomodulation via CD8A / LCK / LAT signaling in the liver model of piglets.

A multi-omics atlas of testicular development in Leiocassis longirostris: dynamic regulation of spermatogenesis.

Focused Ultrasound Stimulation on the Spleen Ameliorates DSS-Induced Neuroinflammation Through the Notch Signaling Pathway.

Comparative transcriptomic analysis across ovarian developmental stages in Sepiella japonica provides novel insights into its molecular regulatory mechanisms.

Acral melanocytic tumors are a common cutaneous neoplasm with malignant potential. Early and non-invasive biomarkers for identifying malignant transformation remain lacking. Exosomal microRNAs (miRNAs) have shown great potential as stable diagnostic indicators in various cancers. To explore the differential expression and clinical significance of serum exosomal miRNAs in patients with acral melanocytic tumors. Exosomes were identified by transmission electron microscopy, nanoparticle tracking analysis, and Western blot. MiRNA sequencing was used to screen differentially expressed miRNAs. Target genes were predicted and analyzed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. A total of 69 differentially expressed miRNAs were identified, of which 14 were up-regulated and 55 down-regulated. Bioinformatics analysis showed that target genes were mainly enriched in cancer-related pathways and the MAPK signaling pathway. Acral melanocytic tumor‑related miRNAs are stably present in serum exosomes and may serve as potential non-invasive biomarkers for the early detection and auxiliary diagnosis of acral melanoma.

Network-based toxicological analysis of core targets and pathways of bisphenol A-driven hepatocellular carcinoma.

Antidepressant effects of combined eucommia-gastrodia extract via modulation of the HIF-1α-EPO/cAMP-CREB-BDNF pathway: An integrated network pharmacology and in vivo study.

Study on chronic toxicity, metabolic profiles and potential mechanisms of Aristolochiae Fructus in rats.

Disposable plastic-alcohol leachates as emerging Neurotoxicants: Evidence for the mir-330-3p/Acsl1 pathway in cognitive performance.

APLNR reduction in kidney-muscle crosstalk in renal model recovered by exercise and STAT3 inhibition.

To investigate the molecular mechanism of Qianlong Shutong formula (QLSTF) in treating benign prostatic hyperplasia (BPH) through the regulation of metabolism and protein function. An integrated metabolomics and proteomics analysis was conducted using a BPH animal model. The animals were divided into a control group, a model group, and a QLSTF intervention group. Following drug intervention, metabolite extraction and protein sample processing were performed. Metabolomics results indicated the presence of metabolic disorders in the model group, which were significantly ameliorated by QLSTF through the regulation of relevant metabolic pathways. Quantitative proteomic analysis identified multiple differentially expressed proteins. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Clusters of Orthologous Groups (COG) enrichment analyses revealed that these proteins are involved in biological processes such as cell proliferation, apoptosis, and inflammatory responses. Correlation analysis demonstrated a synergistic relationship between metabolite levels and protein expression. Integrated analyses revealed metabolic disorders and differential protein expression, providing multiomics evidence for QLSTF's mechanism. QLSTF alleviates BPH by modulating metabolic pathways and protein networks, providing a molecular basis for TCM in BPH treatment and suggesting future research.

Antarctic sea anemones are dominant benthic predators, yet their molecular adaptive strategies to polar environments remain poorly understood. Urticinopsis antarctica is one of the most abundant Antarctic actiniarians, but until now it has lacked any integrated molecular characterisation This study provides the first comprehensive analysis combining de novo transcriptomics with histological and cytological observations to investigate immune and stress-response mechanisms in this azooxanthellate anthozoan. Gene Ontology and Pfam-based analyses revealed a broad and evolutionarily conserved innate immune repertoire, including Toll-like receptor, NF-κB, JAK-STAT and NOD-like receptor pathways, together with numerous transcripts of pattern-recognition domains such as TIR, NACHT, LRR and C-type lectins. Integration with signal peptide prediction demonstrated a significant enrichment of immune- and stress-related genes within the secretome, indicating a strong bias toward extracellular defence strategies. Histological analyses revealed a typical a diploblastic organization with abundant cnidocytes, mucous cells and migratory amoeboid cells, consistent with an epithelial-centred immune system. The absence of algal symbionts was confirmed at both molecular and tissue levels. Together, these findings indicate that U. antarctica maintains a complex, flexible immune and stress-response architecture despite extreme thermal stability and resource limitation. This new molecular and cellular baseline establishes U. antarctica as a valuable reference for understanding resilience, cold adaptation, and the evolution of immunity in polar anthozoans, and provides a foundation for future functional and experimental work on the responses of Antarctic benthic species to environmental change.

Patients with ulcerative colitis (UC) exhibit an elevated risk for post-traumatic stress disorder (PTSD) and frequently demonstrate severe symptomatology, suggesting a gut-brain axis connection. UC-associated chronic inflammation and immune dysregulation can influence central nervous system function via neuroimmune signaling, potentially reshaping molecular and transcriptional programs within fear-related neural circuits. However, the molecular mechanisms underlying this clinical association remain poorly characterized. We analyzed PTSD and UC microarray data from the Gene Expression Omnibus (GEO) following background correction and normalization. Disease-associated genes were identified through differentially expressed gene (DEG) analysis and weighted gene co-expression network analysis (WGCNA), followed by gene set enrichment analysis (GSEA), Gene Ontology (GO) enrichment, CIBERSORT immune profiling, and protein-protein interaction (PPI) analysis. Key targets were validated in intestinal tissue from a murine model of UC and blood samples from a murine model of PTSD. A combined UC-PTSD mouse model was established for behavioral validation; viral knockdown targeting the prelimbic cortex (PL) was employed to test candidate genes functionally. We identified shared molecular pathways and core genes linking UC and PTSD. The comorbid model demonstrated that UC intensifies PTSD-like behaviors. Furthermore, Knockdown of Glrx in the PL alleviated fear retrieval following UC induction, establishing Glrx as a mechanistic node along the gut-brain axis in UC-associated PTSD vulnerability.

Combining network pharmacology and transcriptomics to validate and explore Shenqiyichang decoction in treating colorectal cancer by NQO1 inhibition, ROS activation and Wnt/β-catenin signaling pathway.