Extract Gel Research Articles

Making of “JE-LI” Liquid Hand Soap to Prevent of Covid-19 Spreading

At pandemic of Covid-19, the government declare health protocol that diligently washing hand using soap to helping prevent Covid-19 spreading. The aim of devotion activity was giving of making “Je-Li’ liquid hand soap training to students n teachers of SD Negeri 29 Banyuanyara, kecamatan Sanrobone, kabupaten Takalar. This soap was reached by lime extract and aloe vera gel that affective as antibacterial and moisturizer. This activities consist of giving material and making soap training, the last, evaluate their understanding about the material and training with quiz. Our partner consist of 21 students of class six and 8 teachers. The result that they have understanding and skill to making liquid hand soap to keep clean of hand and to avoid Covid-19 spreading. 

Kersen leaf extract gel (Muntingia calabura L.) to Improve the healing of perineal wounds in postpartum mothers

Background: Delivery processes experience 90% perineal rupture—worldwide. Previous research on test animals proved the effect of cherry leaf gel on wound healing can kill microorganisms, increase collagen, and repair wound tissue. However, no research has been found on healing perineal wounds in postpartum mothers using cherry leaf gel, so this research is necessary.Purpose: Making and testing kersen leaf extract gel to improve perineal wound healing for postpartum women.Method: This is a quasi-experiment with a pre-post-test with a control group design. The sample consisted of 26 postpartum women with perineal injuries grade 2, divided into two groups (Intervention and control). The intervention was given a combination of kersen leaf gel 25% with antibiotic 2x1 for seven days, and the control group was only given the antibiotic. Perineal wound healing was assessed using the REEDA score. The data analysis used the Mann-Whitney test.Results: The results showed that the average REEDA score was lower in the intervention group than in the control group on day 3 (5.00±1.155 vs. 5.85±0.801; p=0.038), day 5 (2.31±0.650 vs. 3.23±1.013; p=0.029), and day 7 (0.23±0.439 vs. 1.15±0.987; p=0.039).Conclusion: Kersen leaf extract gel is effective in improving perineal wound healing in postpartum women.

Cabbage leaves extract gel for breast engorgement during lactation

Background: National surveys have found that painful breasts are the second most common reason to stop breastfeeding, with most mothers reporting the most intense breast engorgement after discharge from the hospital or health center. Many solutions to deal with breast swelling include a cabbage extract compress. In previous studies, cabbage leaves can treat breast swelling, but nothing has been made in more practical topical preparations such as gels.Objective: Making and testing cabbage gel to decrease breast engorgement.Methods: This is an experimental study with a pretest-posttest design. Respondents totaled 32 breastfeeding mothers on the second to tenth day, 16 were given cabbage gel 3.75 grams, and 16 were given breast care for 10 minutes. The intervention was carried out in the morning and evening for four days, and breast engorgement was measured using the Six Point Engorgement Scale (SPES) instrument. Data were analyzed using the Mann-Whitney test.Results: The results of breast treatment using cabbage gel were better than standard breast care, the swelling breast score decreased from the first to the fourth day from an average of 4.94 to 1.00, and the cabbage gel treatment was more effective for three days (p<0.05).Conclusion: Cabbage gel is suitable for reducing breast engorgement during breastfeeding.

Surfactant-Assisted Purification of Hydrophobic DNA Nanostructures.

Purification of functional DNA nanostructures is an essential step in achieving intended functions because misfolded structures and the remaining free DNA strands in a solution can interact and affect their behavior. However, due to hydrophobicity-mediated aggregation, it is difficult to purify DNA nanostructures modified with hydrophobic molecules by conventional methods. Herein, we report the purification of cholesterol-modified DNA nanostructures by using a novel surfactant-assisted gel extraction. The addition of sodium cholate (SC) to the sample solution before structure folding prevented aggregation; this was confirmed by gel electrophoresis. We also found that adding sodium dodecyl sulfate (SDS) to the sample inhibited structural folding. The cholesterol-modified DNA nanostructures prepared with SC were successfully purified by gel extraction, and their ability to bind to the lipid membrane surfaces was maintained. This method will facilitate the purification of DNA nanostructures modified with hydrophobic molecules and expand their applicability in the construction of artificial cell-like systems.

Formulation and Evaluation of Lime Peel Extract (Citrus aurantiifolia) Gel with Semi-Refined Carrageenan and Glucomannan as Gelling Agent

Lime peel extract contains quercetin with sunscreen activity. It could make into gel preparations, which has a transparent semisolid and generally gives a cooling sensation to the skin. A combination of semi-refined carrageenan and glucomannan as a gelling agent can be an alternative to form the strength and elasticity of the gel mass. This study aimed to determine the effect of concentration variations of gelling agents on the physical evaluation of gel preparation results and the optimal formulation for lime peel extract gel as sunscreen. Lime peel extract would make into gel preparation with variations in the concentration of the gelling agent, which are 1%, 1.25%, 1.5%, and 1.75%. Afterward, the physical evaluation included organoleptic, homogeneity, pH, viscosity, spreadability, and adhesion tests. The organoleptic, homogeneity, pH, and adhesion test results show that all formulations meet the requirements. Viscosity test results obtain the best formula III with 3940,00 cPs at 60 rpm . The results of the dispersibility test show the best formulas I and II with 6,10 cm and 5,50 cm. Based on the data analyzed, variations of gelling agents affect the pH, viscosity, spreadability, and adhesion test results. The optimal lime peel extract gel formulation has not defined from the physical evaluation results.

Development of chitosan/gelatin/starch composite edible films incorporated with pineapple peel extract and aloe vera gel: Mechanical, physical, antibacterial, antioxidant, and sensorial analysis

AbstractIn this study, chitosan (C)/gelatin(G)/starch(S) composites incorporated pineapple peel extract (PPE) and aloe vera gel (AVG) were prepared by the casting method as antioxidant agents. Mechanical, water vapor permeability (WVP), moisture content, solubility, opacity, and color properties of the effects of PPE and AVG at different concentrations (0%, 10%, and 30%) were investigated. The prepared films were characterized by Fourier transform infrared spectroscopy in attenuated total reflection (ATR‐FTIR), X‐ray diffraction (XRD), Thermal gravimetric analysis (TGA), Scanning electron microscope (SEM), mechanical, water barrier, opacity, and color. PPE and AVG extract incorporated edible films improved thermal stability of edible films. Tensile strength decreased with the addition of extracts; the maximum tensile strength was 8.15 MPa for CGS film. The WVP of the films increased with the increasing PPE and AVG concentration. As a biological activity, the films inhibited the growth of gram‐positive (Staphylococcus aureus) and gram‐negative bacteria (Escherichia coli). With the addition of PPE and AVG to the CGS film, its antioxidant properties were enhanced. In addition, the prepared films were applied for strawberry coating both in the refrigerator and at room temperature. Consequently, these edible‐coated films can be used for active food packaging/coating to extend the shelf life of fruits.

Hardness analysis of remineralization primary teeth enamel after the application gel of duck eggshell extract (anas platyrhynchos domesticus) with consentration 20% and 40% in vitro

Background : Children are a population that has a high potential to be exposed to caries. Calcium deficiency due to demineralization of the enamel can cause a decrease in hardness during caries pathway. It can be anticipated with the application of re-mineralizing materials. Duck eggshells are contain high amount of calcium. This research aimed to examine the effect of duck eggshell extract gel (Anas plathyrhynchos domesticus) on the enamel hardness of primary teeth. Methods : This research is an experimental laboratory type using a pre and post-test control group design. There were 3 treatment groups with 4 samples in each group, which consisted of application of duck eggshell extract gel (Anas plathyrhynchos domesticus) with a concentration of 20% and 40%, and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste. Sample application was carried out 42 times in 14-day intervals. Then the sample was measured with vickers microhardness tester. The data were processed by using Paired T-Test, Kruskal-Wallis, and Post Hoc Mann-Whitney statistical tests. Results : showed that there were significant differences in the samples before and after the application of duck eggshell extract gel (Anas plathyrhynchos domesticus) (p<0.05). Significant differences were also found in the three groups (p<0.05). The group that experienced the highest increase in hardness was the 20% duck eggshell extract gel (Anas plathyrhynchos domesticus) treatment group. Conclusion : was that duck eggshell extract gel (Anas plathyrhynchos domesticus) with a concentration of 20% was effective in increasing the hardness of primary tooth enamel

ANTIBACTERIAL ACTIVITY OF LIQUID CRYSTAL NANOPARTICLES GEL OF BINJAI LEAVES METHANOL EXTRACT (MANGIFERA CAESIA JACK. EX. WALL.) AGAINST PROPIONIBACTERIUM ACNES

Objective: This study aims to compare the anti-Propionibacterium acnes activity between the optimum formula of gel liquid crystal nanoparticles of Binjai leaves methanol extract and the gel of extract without the liquid crystal nanoparticles system.
 Methods: Preparation of liquid crystal nanoparticles using top-down methods with 6% Capmul-GMO 90 and 5% Plantacare 818. Preparation of the optimum gel formula using 7% Viscolam Mac 10 as a gelling agent. The antibacterial activity was evaluated by cup plate technique and clindamycin was used as a positive control.
 Results: The results obtained are liquid crystal nanoparticles based gel of methanol extract of Binjai leaves produces a strong category as anti-Propionibacterium acnes with an average inhibition of 15.33±1.2413 mm and the gel of extract without the liquid crystal nanoparticles system only produces 13.53±1.241 mm.
 Conclusion: The gel of Binjai leaves extract with a liquid crystal nanoparticles system has a higher antibacterial effect on Propionibacterium acnes than the gel of extract without the liquid crystal nanoparticles system.

Formulation and Physical Stability Test Preparation of Anti-Acne Gel Extract of Tebu Leaves (Saccharum officinarum (Linn) as Antibacterial Against Staphylococcus Epidermis

Increased processing of Tebu as sugar causes the resulting waste, namely the shoots of Tebu leaves. The phenolic and flavonoid compounds in Tebu are reported to have strong antioxidant, anti-inflammatory, antimutation and tyrosine inhibitor activities. Infectious diseases are diseases caused by the presence of pathogenic microbes, one of which causes infection, namely bacteria. Utilization of Tebu waste is expected to produce alternative products in the form of antibacterial preparations, especially anti-acne. Staphylococcus epidermis is one of the bacteria that causes infection on skin with acne. This study aims to determine the physical stability of the preparation Tebu leaf extract anti acne gel and its antibacterial activity against Staphylococcus Epidermis bacteria. Methods: This research design is an experimental method, making Tebu leaves extract by weighing as much as 400 grams of fine powder of Tebu leaves then put in a maceration container, closed then added 70% 2 liters of ethanol until completely submerged and stored for 24 hours protected from direct sunlight while stirring occasionally. Then filtered, separated the dregs and filtrate. The dregs were re-extracted with the same amount of new 70% ethanol for 2 days. The obtained 70% ethanol filtrate was evaporated to obtain a thick extract using a Vacuum Rotary Evaporator. Tebu leaves extract gel was made at a concentration of 5%, 10%, 15% and an antibacterial test was carried out using the well diffusion method. Results: The results of the physical stability test of the acne extract cream showed that the results met the requirements both in terms of pH, dispersion test, homogeneity. The results of the antibacterial test of Tebu leaves extract showed the presence of a zone of bacterial inhibition which was indicated by the presence of a clear area around the well. Conclusion: The results of the evaluation showed that the Tebu leaves extract gel met the requirements for the physical quality of the gel preparation, both organoleptic, pH, viscosity, adhesion, and spreadability. Tebu leaves extract gel was able to inhibit the growth of S. epidermis bacteria with the best concentration in 3rd formula, consentration 15% Tebu leaves extract gel.

UJI AKTIVITAS EKSTRAK LABU AIR (Lagenaria siceria) TERHADAP LUKA BAKAR TIKUS JANTAN GALUR WISTAR

Water pumpkin (Lagenaria siceria) is a useful plant in Indonesian. People used water pumpkin flesh as a drug wound, especially as wound burning. This study was prove the activity of water pumpkin flesh extract as wound burning in male rats Wistar strain. The method in this research used experimental. This study used 25 male rats Wistar strain divided into 5 groups. The positive control group was given bioplacenton, the negative control group was given CMC-Na base gel, the other group was given water pumpkin gel extract at doses 2.5%, 5% and 7.5%. compount of water pumpkin extract are flavonoid and saponin. This compount have activity as a burn medicine in male rats wistar strain. At activity level 2.5% has activity 40,06%, at dose 5% has activity 49,75%, and at dose 7.5% has activity 60,11%. Water pumpkin gel extract at 2.5%, 5%, 7.5% dose had different activity with negative control group with CMC-Na gel base, but no significant difference (sig> 0,05) 25%, 50%, 75%.

The Anti-Candida and the Cytotoxic Activity of Aloe Vera Gel Extracts

Purpose: The research paper studies the in vitro anti-candida activity and cytotoxic effects of Aloe vera (Linn) leaves extract against melanoma and normal cell lines. Subjects and Methods: The traditional Soxhlet apparatus was used to prepare the crude plant extract using organic solvents. The agar well diffusion method was used to explore the in vitro anti-candida, and the MTT assay was used to assess the cytotoxic activity. Results: With IC50 values of 250 g/ml and more than 1000 g/ml, respectively, crude extract demonstrated anti-Candida activity against Candida ((kefyr, glabrata, and albicans) in varying potencies and cytotoxic effect in B16 cell line and vero cell line. Conclusions: This study unequivocally establishes that the crude extract of Aloe vera leaves with bioactive metabolites is capable of supplying high-quality biological material for anti-Candida and anti-cancer therapeutic programs.

ANTI-MICROBIAL ACTIVITY AND DIABETIC WOUND HEALING EFFECTS OF PSIDIUM GUAVA AND MELASTOMA MALABATHRICUM LINN LEAVES GEL IN RATS (SPRAGUE DAWLEY)

The purpose of this study was to determine the potential of guava and melastoma malabathricum linn leaves mixed gel activity as anti-microbial and wound healing in experimental rats (Sprague dawley). The thick extracts of melastoma malabathricum linn and guava leaves were made in a gel dosage form with a concentration of 4% and 6% and clindamycin as a positive control. This study used experimental laboratory methods, and the population in this study were white male rats and was carried out in the Pharmacology and Pharmaceutical Laboratory, National Institute of Science and Technology. The pharmacological activity was tested in the form of a gel dosage formula, and the rats were made diabetic through an inducing agent, namely alloxan, then the potential of the extract was seen in repairing diabetic wounds in male rats. Melastoma malabathricum Linn and guava Leaves extract gels have an effect in healing diabetic-induced rat wounds using alloxan. This can be seen from the change in the diameter of wound healing. Secondary metabolites of both plant materials are thought to provide anti-microbial activity and stimulate wound healing which helps epithelial cells and tissues so that they regenerate. The chemical content in the extracts of the test preparation has different mechanisms in helping the healing process.
 

Seaweed Extract (Eucheuma cottonii) Antibacterial Potency Test in Gel Form On Staphylococcus aureus and Staphylococcus epidermidis Bacteria

Background: Incorrect medicine usage in treating acnes (acne vulgaris) may cause unwanted side effects. Teratogenic effects due to retinoid use in pregnancy and breastfeeding women as well as antibiotic resistance on account of irrational antibiotic use are a few of the effects that may emerge. Natural ingredients such as seaweeds (Eucheuma cottonii) formulated in topical form is an alternative to solve this problem. Objective: To ascertain seaweed extract (Eucheuma cottonii) antibacterial potency test in gel form on staphylococcus aureus and staphylococcus epidermidis bacteria. Method: This research is a true experimental research with posttest-only group design. Treatment groups are clindamycin gel positive control, sterile aquadest negative group, and seaweed infused extract group with 5%, 10%, 15%, and 20% concentrations with Staphylococcus aureus and Staphylococcus epidermidis bacteria as testing bacteria. Antibacterial testing method is done using disk diffusion method. Raw data is analyzed using Kruskal-Wallis statistical test. Result: The diameter of zone of inhibition of seaweed extract gel on Staphylococcus aureus bacteria on the 20% concentration (4,51 mm), 15% concentration (0,265 mm), 10% concentration (0,05 mm), and 5% concentration (0,22 mm) is categorized as weak antibacterial potential. The diameter of zone of inhibition of seaweed extract gel on Staphylococcus epidermidis bacteria on the 20% concentration (10,37 mm) is categorized as strong antibacterial potential, whereas 15% concentration (3,545 mm), 10% concentration (4,045 mm), and 5% concentration (0,725 mm) is categorized as weak antibacterial potential. Analysis shows that there are significant differences between treatment groups with p value < 0,05. Conclusion: Seaweed extract (Eucheuma cottonii) in gel form has an antibacterial potency towards Staphylococcus aureus and Staphylococcus epidermidis bacteria.
 Keywords: Seaweed, Antibacterial, Acne, Gel

A New Ceramide from Cissus Aralioides Baker (Vitaceae) and its Antimicrobial Activity.

Purification through repeated column chromatography over silica gel and Sephadex LH-20 of the ethanol extract of the stems of Cissus aralioides (Baker) Planch. resulted in the isolation of a new ceramide, aralioidamide A (1), along with five known compounds (2-6). Their structures were determined by the extensive analyses of their spectroscopic (1D and 2D NMR) and spectrometric data, and comparison with those reported in the literature. Aralioidamide A (1) displayed weak antibacterial activity (MIC=256 μg/mL) against Bacillus subtilis, Staphylococcus aureus and Shigella flexneri and was inactive (MIC>256 μg/mL) against the tested fungi.

Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signaling

BackgroundGel extracted from the succulent inner leaf mesophyll of species from the plant genus Aloe have been used in traditional medicine for centuries as a topical treatment for a wide range of skin conditions. Aim of the studyThe aim of this study was to investigate the potential anti-inflammatory properties of leaf gel from 23 different Aloe species. In this study we have focused on the pathways activated by the toll-like receptors TLR4 (activated by LPS), and TLR2/TLR6 (activated by FSL-1) by measuring transcriptional activity of NF-κB. Materials and methodsGel extractions from the inner leaf mesophyll material of 23 different Aloe species, pure acemannan obtained from Aloe vera, and pH. Eur. reference material from Aloe vera and Aloe ferox, were tested on the cell line J774-Dual. NF-κB activity was measured after 24 h. ResultsWe found that 8 out of the 23 tested Aloe gel extracts significantly decreased LPS-induced NF-κB-activity, but no significant effect was seen with FSL-1. ConclusionBased on these findings we can confirm that extracted gel from some of the tested Aloe species but not all, have anti-inflammatory properties.

The Effect of Aloe Vera Topical Gel Extraction on Tgf-Β1 Expression in Thermal Burn Eyelid Grade II Rats Model

Grade II eyelid burns are burns that reach the epidermis and part of the dermis of the palpebrae and can have impaired wound healing. TGF β1 has the broadest spectrum of action, affecting all cell types involved in all stages of wound healing. TGF β1 increases rapidly after injury and reaches peak levels three days after injury, which coincides with the peak of the inflammatory phase of wound healing. Decreased expression of TGF β1 is associated with impaired wound healing. Aloe Vera has long been known in Indonesia as a healing plant. Aloe Vera extract contains a variety of bioactive compounds that play a role in stimulating wound healing. The administration of Aloe Vera extract on thermal burns aims to reduce inflammation, increase collagen content, and increase the rate of re-epithelization. The administration of Aloe Vera gel extract allows faster healing of thermal burns and rebuilds the vascularity of the wound tissue. Objective of this study is to determine the difference between the expression of Transforming Growth Factor Beta 1 (TGF β1) in a rat model of thermal burn injury grade II of the eyelids treated with topical Aloe Vera gel extract with the concentrations of 20%, 40%, Balanced Salt Sodium (BSS) and a control group that was not given burn intervention. An experimental study with the post-test-only approach with a control group design. 24 Rats have induced grade II eyelid thermal burns, divided into four groups: Normal (without treatment), negative control (BSS), given Aloe vera 20%, 40%. Then TGF β1expression was quantified using the Image J application. The Topical application of Aloe vera gel can increase the expression of TGF along with the increase in dose. The highest rise in TGF expression was found in the group with 40% Aloe vera gel, followed by 20% Aloe vera gel compared to the negative control (BSS Balanced Salt Solution). Aloe Vera can increase TGF expression in a rat model of grade II palpebral burn injury and is dose-dependent.

Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats.

Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nutrients and bioactive compounds. In this study we evaluated the preclinical toxicology of crude extract and vaginal gel obtained from the leaves of E. uniflora (5%, 10%, and 15%) aiming to provide safety for its use in the treatment of vulvovaginitis. Both formulations were applied to the vaginal cavity for 14 days. Detailed observations of the vaginal region, including pruritus, swelling, irritation, burning, pain, and vaginal secretion, as well as the estrous cycle were evaluated. On the fifth day, blood samples were obtained from the supraorbital plexus for biochemical and hematological analyses. The animals were subsequently euthanized. All animals underwent necropsy and macroscopic examination of the vaginal mucosa and reproductive system. A histological examination was also performed. No clinically significant changes were detected during the entire experimental period. All biochemical, hematological, or histopathological parameters were within the normal range for the species. The data obtained allow us to suggest that the E. uniflora vaginal formulations are safe in this experimental model.

EFFECTS OF MANGOSTEEN SKIN GEL EXTRACTS ON INCREASING THE NUMBER OF FIBROBLASTS IN THE HEALING PROCESS OF RATS PERIODONTITIS

Background: Periodontitis is one of the most common periodontal diseases in Indonesia. Inflammation and the bacteria that cause periodontitis can be treated by providing additional therapy in the form of a gel by the dentist. Mangosteen peel extract gel contains xanthones which function as antibacterial and anti-inflammatory that will accelerate the inflammatory phase. This study aims to determine the effectiveness of a gel made from mangosteen peel extract to increase the number of fibroblast cells on the healing process of periodontitis in Wistar rats. Methods: This is an experimental laboratory with post test only design. The treatment group composed of 50% mangosteen peel extract gel, 75% mangosteen peel extract gel, 25% metronidazole gel, and metronidazole plus mefenamic acid gel which were applied to Wistar rats after periodontitis induced. Results: The research data were analyzed by One Way ANOVA and exhibited a significance score of p = 0.000 (p<0.05) which indicated a significant difference. In the inter-group test with Bonferroni post hoc, it was found that there was no significant difference between the 75% mangosteen peel extract gel group and 25% metronidazole gel group with a score of p = 0.709 (p>0.05). Conclusion: The conclusion of this research showed that mangosteen peel extract gel was effective in increasing the number of fibroblast cells in the recupiration process of periodontitis

An improved method for the production of biogenic silica from cornhusk using sol–gel polymeric route

AbstractPorous silica was synthesized from cornhusk using the sol–gel polymeric route and compared with ash obtained from the direct combustion process under laboratory conditions. The unmodified ash from the direct combustion process was dissolved in NaOH for 1 h to form sodium silicate, which was subsequently hydrolyzed with citric acid to yield a silica xerogel. The obtained xerogel was characterized using inductively coupled plasma–optical emission spectrometry (ICP-OES), Fourier transforms infrared (FTIR) spectroscopy, X-ray diffraction (XRD), simultaneous thermal analysis (STA), gas sorption techniques to determine their elemental constituents, functional groups, crystalline phases, thermal stability, and porosity, respectively. The results showed that the synthesized silica xerogel exhibited porous network structures with a high-specific surface area and mesopore volume of 384 m2/g and 0.35 cm3/g, respectively. The pore size distribution revealed a complete transformation of the pore network structures of the unmodified ash from a monomodal to a bimodal pore system, with micro- and mesopore peaks centered around 1.5 and 3.8 nm, respectively. The ICP-OES results showed that the silica content significantly increased from 52.93 to 91.96 wt.% db after the sol–gel treatment. XRD diffraction confirmed the amorphicity of the silica particles obtained from the sol–gel extraction method. In addition, the STA data showed that the silica xerogel has high thermal stability compared to the unmodified ash, as the latter exhibited poor thermal stability and low textural properties. The high surface area and narrow pore cavity size distribution of the porous silica xerogel make it an ideal substrate for catalysts and an excellent template for growing other nanoparticles within the pores.

Efficient optimization and development of two methods for the determination of acrylamide in deep-frying oil by liquid chromatography-tandem mass spectrometry: Application of multifactor analysis assessment strategy.

A new multifactor analysis assessment strategy was developed for evaluating, optimizing, and comparing analytical techniques for acrylamide in frying oils. Based on five indices (absolute recovery, absolute matrix effect, the intensity of the full ion scan, and the precursor ion scan to m/z 184 and m/z 241), the proposed strategy was performed with radar analysis, relative contribution analysis, and the entropy-weighted technique for order performance by similarity to ideal solution analysis. Two novel methods based on quick, easy, cheap, effective, rugged, and safe extraction methodology and gel permeation chromatography-liquid-liquid extraction followed by liquid chromatography-tandem mass spectrometry have been developed for the analysis of acrylamide in frying oils. Two methods were suitable for rapid and sensitive analysis of acrylamide in oils in different laboratories, with a limit of quantitation at 2μg/kg, and the average recovery ranging from 92.5% to 107.8%, with relative standard deviations below 10%. When considering automation efficiency and matrix effects, gel permeation chromatography is the most efficient method, whereas the other method has an advantage when analyzing large samples. The developedmethods were used in a pilot study to analyze frying oils with acrylamide content below 9.82μg/kg, showing that the repeated frying process did not produce significant content of acrylamide in oils.