Gauze Research Articles

Mesoporous bioactive glass nanoparticles with biomineralization activity for surgical hemorrhage control

ABSTRACTBioactive glass nanoparticles (BGNs) are good bioactive materials for clinical treatment because of their unique composition and silica-oxygen network structure. Herein, we designed and reported a monodisperse mesoporous bioactive glass nanoparticle with biomineralization activity for surgical hemorrhage control. The results indicated that the BGNs have excellent biomineralization ability. In vivo and in vitro hemostasis studies suggested that BGNs have superior hemostatic properties to GZ (gauze) and GS (commercial gelatin sponge) and validated in vitro thrombosis, platelet adhesion, cytocompatibility and blood compatibility. The particles activated both endogenous and exogenous physiological coagulation pathways and could activate platelets and release Ca2+ to participate in the clotting pathway. Besides, the BGNs have good biocompatibility in vivo. These findings demonstrated that the potential of small diameter mesoporous bioactive glass nanoparticles is likely useful for bleeding control as potential hemostatic agents.

Investigation of gauze and medical bottle co-pyrolysis on the product formation, reactivity, and reaction pathway of char, liquid oil, and gas.

Effective in-site treatment of medical waste has become a weak link in hospitals. Pyrolysis technology is a treatment method for medical waste that can enable rapid disposal in hospital settings and relieve environmental pressure, while also producing high-value products and reducing disposal costs. In this work, the effects of feedstock ratio and temperature on product yield and components of gauze (GA) and medical bottles (MB) co-pyrolysis have been investigated. The higher yield of solid products was obtained by co-pyrolysis of GA and MB at 400 ℃. With the addition of MB and an increase in temperature for the co-pyrolysis of GA and MB in a similar ratio, the pyrolysis oil and gas yields gradually increased. According to GC–MS analysis, co-feeding 75% MB to GA improved the alcohol content from 33.21% to a maximum yield of 59.8% at a pyrolysis temperature of 700 ℃. The content of aliphatic hydrocarbon reached 38.68% when the pyrolysis temperature and MB addition ratio were 700 °C and 75%, respectively. The GC data shows that the main gas components of co-pyrolysis of GA/MB were CH4 and H2, while the pyrolysis of pure GA or MB resulted in CO or CO2. Additionally, the solid carbon products obtained have an excellent pore structure. This strategy can benefit medical waste control and resource utilization for the low-cost disposal of medical waste and the acquisition of high-value resource products.

The Interconnection between Masks and Oral Health

In healthy individuals, the immune system processes pathogens with a natural immune response that is mild and non-lethal [1];however in diseased tissues like the gum disease (Periodontitis), the immune system is compromised- which overreact to affect other organs and tissues During the Spanish flu pandemic in 1918-1919, common people, not just medical professionals were mandated to wear gauze face mask to limit the spread of the disease, and non-compliance led to tines, public ridicule and sometimes jail time [3] The oral cavity supports about 700 bacterial which are involved in the differentiation and maturation of immune cells, maintenance of the immune system and balance between pro-inflammatory and anti-inflammatory processes in the immune cells which are basically Neutrophils, T cells, B cells, monocytes, macrophages dendritic cells

Clinical analysis of medical hemostatic materials for prevention and treatment of subcutaneous hydrops after breast cancer operation

Objective To observe the effect of medical instant hemostasis gauze combined with filament speed instant gauze on the drainage and flap healing after modified radical mastectomy. Methods From August 2015 to August 2016, a total of 80 patients with modified radical mastectomy for breast cancer admitted to Huanxing Tumor Hospital, Chaoyang District, Beijing were selected.According to the random number table method, 80 patients who were ready for modified radical mastectomy for breast cancer were randomly divided into study group (40 cases) and control group (40 cases). Two kinds of hemostatic materials (medical hemolytic hemostatic gauze combined with fibril quick hemostatic gauze) were applied to the surgical wounds in the study group during the operation, while no medical hemostatic materials were used in the control group during the operation, and the other treatment was the same as that in the study group.Total drainage volume and drainage tube removal time were compared between the two groups 1 to 5 days after operation. Results There were no statistically significant differences in the age, body mass index, and effusion production between the two groups (all P>0.05). The total drainage volume of the study group was (289.23±5.36) ml, and the total drainage volume of the control group was (492.15±8.56) ml.The difference between the two groups was statistically significant (t=8.543, P<0.05). The drainage time of the study group was (6.24±1.23) days, and the extraction time of the control group was (10.12±2.21) days.The difference between the two groups was statistically significant (t=6.203, P<0.05). Conclusion In addition to hemostatic function, using absorbable hemostatic gauze combined with surgicel fibrillar during the surgical process can significantly reduce postoperative subcutaneous fluid accumulation. Key words: Medical hemostatic material; Breast Cancer; Subcutaneous fluid accumulation

The application timing and curative effect of carprostol tromethamine in the treatment of postpartum hemorrhage due to uterine atony

Objective To explore the application timing of carprostol tromethamine in the treatment of postpartum hemorrhage due to uterine atony, and to observe the clinical efficacy. Methods From January 2016 to June 2018, 62 patients with uterine atony and postpartum hemorrhage admitted to the Second People′s Hospital of Lianyungang were selected as study objects, and they were randomly divided into two groups according to the digital table, with 31 cases in each group.In the control group, after the delivery of the fetus, 20U of oxytocin was intravenously injected.If there was active hemorrhage on the dissection surface of the placenta, 40U of oxytocin was continued.If there was still hemorrhage, the uterus was injected with 250μg of carprostol tromethamine.In the observation group, after the delivery of the fetus, 20U of oxytocin was intravenously injected.If there was active hemorrhage on the dissection surface of the placenta, 250μg of carprostol tromethamine was injected immediately.If the patients in the two groups still had active bleeding in the uterus after the above treatments, the uterine cavity was filled with gauze or uterine artery ligation was performed to stop bleeding.The blood loss during the operation, 2h and 24h after surgery were recorded and compared between the two groups.The 24h postpartum hemoglobin(Hb) decreased value, treatment efficacy and the incidence of adverse reactions were also recorded and compared between the two groups. Results The intraoperative blood loss was (1 066.1±71.3)mL in the observation group, which was significantly lower than that in the control group [(1 163.6±70.9)mL], the difference was statistically significant(t=5.393, P 0.05). The decrease of Hb in the observation group at 24h after birth was (20.80±6.25)g/L, which was lower than that in the control group [(26.90±9.21)g/L], and the difference was statistically significant(t=3.033, P 0.05). Conclusion Postpartum hemorrhage caused by uterine atony can be treated by early use of carprostol tromethamine, which can effectively reduce postpartum bleeding and promote the rehabilitation of patients.The curative effect is better than oxytocin, and it is worthy of clinical application. Key words: Postpartum hemorrhage; Uterine inertia; Cesarean section; Carboprost tromethamine; Oxytocin; Drug therapy; Treatment outcome; Comparative study

Research of TC-PHBHHx/β-TCP compounds for treatment of dry socket in SD rats

The effects of different compounds on dry socket were evaluated in order to find a new method that can both be antibacterial and osteogenic,providing experimental evidence for future clinical application. Seventy-two male SD rats, with upper left anterior teeth been extracted, were infected by pus to result in dry socket.Seven days later, they were allocated randomly and evenly into 4 groups and received different treatment, i.e. group A: debridement; group B: debridement and filled with iodoform gauzes; group C: debridement and filled with periocline; group D: debridement and filled with TC-PHBHHx/β-TCP. After being treated for 1,4,8 weeks, sequential fluorescent labeling was performed. The animals were sacrificed after the procedure and hard tissue and decalcified sections were harvested for histological and histomorphometrical evaluation. Statistical analysis was performed using SPSS 19.0 software package. At the same time point, the results of osteogenesis in group A, B and C were not significantly different while the results in group D was significantly different from other groups; accordingly, significant new bone formation was observed. At different observation time, the bone area in group B and C were not significant different over time. In group A, little new bone formation was found but surrounded by a large amount of inflammatory cells at 8 week. However, group D showed bone area increasing gradually with time. TC-PHBHHx/β-TCP has significant anti-inflammatory and osteogenic effects. Iodoform gauzes and periocline have good anti-inflammatory results but not significant osteogenic effects.

The Effect Of Calender Temperature And Number Of Fabric Layers On Fabric Performans In Combining Nonwovens With Calender Method

Nonwoven kumaş endüstrisinde, son üründe istenen özelliklere göre lif esaslı tülbent tabakaları başlıca mekanik, ısı ve kimyasal yöntemlerle bağlanarak tekstil yüzeyi haline getirilmektedir. Çevre koruma konusundaki farkındalık, kimyasal maddelerle yapılan doku bağlamanın kullanımı yerine ısı ile bağlama yöntemleri tercih edilmeye başlanmıştır. Isı ile doku oluşturma (kalender) ile doku bağlama yönteminde yüksek üretim hızı, daha iyi fiziksel özellik (kopma ve yırtılma mukavemeti) ve çevre dostu üretim yapılabilmesi bu yöntemi ilgi çekici hale getirmiştir. Dokusuz kumaş üreticileri sürekli olarak üretim hızını arttırmaya ve aynı güç için daha yüksek güç, daha az ağırlık veya yüksek genişletilebilirlik gibi performansı artırmaya çalışıyor. Üretim hızını artırmak için, kalender silindir sıcaklığı yükseltilir, böylece daha az zamanda daha fazla ısı emilir. Nonwoven kumaşlara uygulanan sıcaklık ve basınç değeri kumaşların mukavemetlerine doğrudan etki etmektedir. Bu çalışmada, farklı kalender sıcaklıklarında, ısıl bağlanmanın lif üzerinde sebep olduğu bozunmalar sonucunda elde edilecek spunbond kumaşların yırtılma, kopma mukavemeti ve su sütunu değerleri karşılaştırılmıştır. Deneysel çalışma sonucunda hem nonwoven üreticilerine hem de tüketicilerine kalender yöntemi ile birleştirmede sıcaklık değerlerinin kumaş fiziksel özelliklerine etkisi hakkında yönlendirme yapılmıştır.

Effects of new type oral nursing on oral status among patients with orotracheal intubation in ICU

Objective To explore the effects of new type oral nursing on oral status among patients with orotracheal intubation in intensive care unit (ICU) . Methods Based on convenience sampling, we selected 51 patients with orotracheal intubation and mechanical ventilation in ICU at First People's Hospital of Shangqiu in control group from June 2017 to January 2018, and 55 patients in observation group from February 2018 to October 2018. Control group carried out traditional oral nursing, while observation group adopted the new type oral nursing which used Bass brushing method combined with scrubbing by gauze. Before and after intervention, we compared the score of Beck Oral Assessment Scale, score of halitosis and oral bleeding of patients between two groups. Results There were no statistical differences in the scores of Beck Oral Assessment Scale and halitosis of patients between two groups on admission in ICU (P>0.05) . One week after nursing, the scores of Beck Oral Assessment Scale and halitosis of patients in observation group were lower than those in control group with statistical differences (P 0.05) . Conclusions The new type oral nursing can improve oral status, halitosis and quality of oral nursing among ICU patients with orotracheal intubation. Key words: Intensive care units; Oral nursing; Tracheal intubation; Oral status

The Combination and Development of Experimental Images and New Media Art : Taking Individual Works like “Gauze” as an Example

The Combination and Development of Experimental Images and New Media Art : Taking Individual Works like “Gauze” as an Example

Using gauze to evenly spread starch for hyperhidrosis testing

Using gauze to evenly spread starch for hyperhidrosis testing

Application of jie you shen long-acting antimicrobial material in prevention of pin tract infection in percutaneously pinned pediatric distal humeral fractures

Objective To evaluate the curative effect of jie you shen (JUC) long-acting antimicrobila material (JUC) in preventing pin tract infection in pediatric distal humeral fractures treated with closed reduction and percutaneous Kirschner pin fixation. Methods A total of 160 pediatric patients were given distal humerus fracture surgery by fixation with percutaneous Kirschner pins. After Kischner pin fixation, the pintails and the affected limbs were sprayed with placebo/JUC first, and then wrapped with gauze immersed with placebo/JUC, followed by immobilization with plaster cast at flexion of the elbow. Pin tract infections were evaluated at the time of plaster removal 4-6 weeks after surgery. Results Postoperative Kirschner pin tract infection rate was 1.25% in JUC group, and Staphylococcus aureus was isolated from one patient. The postoperative Kirschner pin tract infection rate was 10.00% in placebo group, including 7 patients infected with Staphylococcus aureus and one patient with Pseudomonas aeruginosa. The infection rate in the JUC group was significantly lower than that in the control group (P=0.016). Conclusion JUC long-acting antimicrobila material can efficiently decrease postoperative pin tract infection after percutaneous Kirschner pin fixation surgeries in pediatric distal humerus fracture patients receiving closed reduction. Key words: Distal humerus fracture; Pin tract infection; Antimicrobial film; Jie you shen; Children

Walnut oil promotes healing of wounds and skin defects in rats via regulating the NF-kB pathway.

The effects of walnut oil on wound healing and skin injury repair was observed in Sprague-Dawley (SD) rats, and mechanism of action was investigated. Normal SD rats were divided into an experimental group and a control group. Each group was observed at4 time points (day [D]3, D7, D14, and D21). In both groups, a skin wound was created on the back of the rats, with the spine as the central axis. In the experimental group, the wound was covered with walnut oil, and then bandaged and fixed with sterile gauze. In the control group, the wound was bandaged with vaseline gauze. At each corresponding time point, the wound area and wound healing time of each rat were examined. Epithelial cells of the wound tissues were observed using haematoxylin and eosin staining and immunohistochemical analysis,and the numbers of inflammatory cells and capillaries were counted. A western blot method was used to detect the expression of nuclear factor (NF)-κB and epidermal growth factor (EGF) in the wound tissues of both groups. Meanwhile, enzyme-linked immunosorbent analysis (ELISA) was used to detect the expression of transforming growth factor (TGF)-β1 and matrix metalloproteinase (MMP)-1 in rat sera. A total of 48 SD rats completed the experiment. Healing time of residual wounds in the experimental group was 10.0±3.5 days, which was significantly shorter than that in the control group (18.0±6.0 days) (p<0.05). The wound healing rates in the experimental group were 54.14 % (D3) and 91.2 3% (D7), whereas those in the control group were 22.12% (D3) and 54.84% (D7 (p<0.05).Histological examinations revealed no epithelial cells on D3, D7, D14, and D21 in both the experimental and control groups. However, the number of inflammatory cells decreased significantly and the number of capillaries increased significantly in the experimental group compared to control (p<0.05). NF-κB expression was significantly lower, EGF expression significantly higher in the in the experimental group. Conversely, ELISA showed a significant increase in the expression of TGF-β1 and MMP-1 in rat sera in the experimental group. So we conclude that walnut oil has significant effects in promoting the healing of skin defect wounds in SD rats.

Experimental Evidence on Performance of an Advanced Gauze Structured Packing under Deep Vacuum Distillation Conditions

Results are presented of total reflux experiments carried out at Fractionation Research Inc. with Montz-Pak A3-500M, an advanced corrugated sheet wire gauze packing, using paraxylene/orthoxylene system at 0.02 and 0.1 bara, respectively. In both cases an expectedly good performance was achieved, ranging from eight stages at low- to four stages per unit bed height at high vapor loads. Interestingly, within preloading region the packing exhibited better efficiency at 0.02 bara, however this was at the expense of somewhat increased pressure drop, and, strikingly, no gain on capacity side. Delft model proved capable of capturing observed trends, but exhibits a pronounced discrepancy with respect to measured efficiency and pressure drop at 0.02 bara, and suggests a higher flooding limit than observed.

Effects of different gauze folding patterns on local hemostasis after peripherally inserted central catheters

Objective To observe the effects of two different gauze folding patterns used in local hemostasis after peripherally inserted central catheters (PICC). Methods A total of 152 patients were selected and divided into two groups according to PICC date sequence as control group of 72 patients using 2.0 cm×2.0 cm little gauze to oppress the puncture point and observation group of 80 patients using 1.0 cm×1.5 cm gauze ball made by ourselves to oppress the puncture point. The oozing of the puncture point was observed in patients of the two groups. Results The hemostasis was better in the observation group than in the control group(χ2=15.88, P<0.01). No limb swelling happened to the patients in the observation group(χ2=58.064, P<0.01). There was statistically significant difference in hemostatic effect between the two groups. Conclusion The sterile gauze ball made by ourselves has a good effect on local hemostasis through oppressing the puncture point without any impact on blood circulation of limbs. Key words: Peripherally inserted central catherter; Sterility gauze ball; Local hemostasis

Clinical research on self-made negative pressure drainage with Nano-Silver dressing in surgical incision infection

Objective To explore the clinical effects of the combination of the self-made negative pressure drainage and Nano-Silver dressings on postoperative infection of incision. Methods A total of 84 patients with post-operative incision infection in the First People's Hospital of Xiangtan City from January 2013 to December 2015 were selected and divided into the observation group and the control group with 42 cases in each by random number table method. The observation group was treated with self-made negative pressure device combined with Nano-Silver dressing at the early stage, while the control group was treated with iodoform gauze dressing. When the wound bed granulation grew well, the two groups were treated with calcium alginate dressing or two stage suture until healed. The inflammatory index, incision growth and therapeutic effect of the two groups were compared. Results A week after treatment, the white blood cell, C reactive protein, incisional pain score and the change cost of the observation group were (7.62±2.15) ×109/L, (19.33±23.46) mg/L, (2.83±0.82) and (570.13±33.88) Yuan, which were all lower than those of the control group, and the differences were statistically significant (t=2.592, 2.695, 7.497, 7.752; P<0.05) . 100% coverage time of the incisional granulation tissue in the observation group was (3.86±1.37) d, and the healing time of the whole incision was (12.02±2.80) d in the observation group, which were both lower than those in the control group, with statistical significance (t=12.669, 6.954; P<0.01) . Conclusions The self-made negative pressure device combined with Nano-Silver dressing is ideal for the treatment of postoperative incision infection, while improving the therapeutic effect of the patients, and reducing the healing time of the incision and the cost of changing the medicine. Key words: Surgical wound; Infection; Vacuum sealing drainage technique; Nano-Silver dressing

Evaluation of the process of venous ulcer healing, treated with babosa gel (Aloe barbadensis Miller): a case study.

This is an experimental case study with the objective of evaluating the cicatricial progression of varicose ulcer treated with the gel in natura of Aloe barbadensis Miller in the period of 83 days. The research included a carrier of varicose ulcer with lesions 2nd and 4th toes D, which was not performing topical drug treatment. The dressing was performed daily following the cleaning protocol with gentle irrigation with 0.9% Sodium Chloride heated and covering with the fresh slug gel extracted from the plant bulb just before the dressing time, maintaining a semi- occlusive with sterile and compressive gauze with bandage. The characteristics of the wound and the evolution of the cicatricial process through the photographic imaging, measurement of the lesion bed with the IMAGE J® and registry of the characteristics of the bed of the lesion, borders and peri ulcer region at 29o, 48o, 66o and 83 days of treatment. The results indicate that the use of phytotherapics promoted the control of inflammatory signs, improved hydration and vascularization of the lesions and peri ulcer regions and cellular regeneration, promoting at the end of the treatment total healing of the ulcer present in the 4th right foot podactylus and important reduction of the area of the other injuries. However, in order to produce evidence for the practice it is necessary to develop other experimental studies with an expanded sample to clarify the benefits and mechanisms of action of the herbal medicine in the treatment of varicose ulcers.

Research progress in hemorrhage control products of U. S. army

From Vietnam War to Operation Iraqi Freedom and Operation Enduring Freedom, hemorrhage remains the leading cause of combat death. The U. S. army has made great strides in research and development of hemorrhage control products and various tourniquets and hemostatic dressings have been fielded on the battlefield. This paper introduces current status of hemostatic products and hemorrhage control products. Currently, there are extremity tourniquets and junctional tourniquets. The hemostatic products include HemCon bandages, QuciClot granular and gauze, Celox gauze, XStat, etc. Meanwhile, the author summarizes three development trends of US military hemostatic products, ie, prehospital application of tourniquets, emphasis on hemorrhage control in junctional regions, and gauze hemostatic adjuncts preference. This review offers materials for fully understanding war wound treatment of U. S. army. Key words: Tourniquets; Hemostatic techniques; U. S. armed forces

Application value of vacuum sealing drainage in the wound repair after resection of pilonidal sinus

Objective To explore the application value of vacuum sealing drainage (VSD) in wound repair after resection of pilonidal sinus. Methods The retrospective cross-sectional study were conducted. The clinical data of 11 patients with pilonidal sinus who were admitted to the China-Japan Union Hospital of Jilin University from April 2015 to June 2016 were collected. All the patients received resection of pilonidal sinus after preoperative antibiotic therapy, and then received postoperative VSD and regular treatments of anti-infection and dressing changes. Observation indicators: (1) treatment situations: surgical procedures, operation time, volume of intraoperative blood loss, gross specimens, results of postoperative pathological examination, postoperative treatment (VSD), growth condition of granulation tissue, duration of hospital stay and treatment expenses; (2) follow-up situations: cases with follow-up, follow-up time, wound healing, postoperative complications and disease recurrence. Follow-up using outpatient examination and telephone interview was performed to detect the wound healing, postoperative complications and disease recurrence up to July 2017. Measurement data with normal distribution were represented as ±s. Measurement data with skewed distribution were described as M(range). Results (1) Treatment situations: all the 11 patients underwent successful surgery for pilonidal sinus. The operation time and volume of intraoperative blood loss were respectively (79±19)minutes and (10±4)mL. Observation of gross specimens of 11 patients: hairs were scattered within the resected lesions in 7 patients, and hairs didn't found in 4 patients. Results of postoperative pathological examination of 11 patients: there were acute and chronic inflammation accompanied with granulation tissues in the subepithelial fibrous tissues, and discrete multinucleated giant cell reaction. Of 11 patients undergoing VSD, 9 received VSD material changes for 3 times, 2 received VSD material changes for once, and wounds were not sutured after VSD removal. The new granulation tissues of 11 patients were found at (5.9±1.3)days postoperatively. The duration of hospital stay and treatment expenses were respectively (33.3±8.3)days and (37 790±8 811)yuan. (2) Follow-up situations: 11 patients were followed up for 12-18 months, with a median time of 14 months. The wound healing time was (41.0±9.0)days. Of 11 patients with follow-up, 10 were in the primary healing, 1 who was complicated with wound infection and abscess of wound deep surface underwent sacral wound incision and drainage, gauze packing and routine dressing change, and then wound healed completely. There was no recurrence in 11 patients during the follow-up. Conclusions The VSD has great application value in managing wounds after resection of pilonidal sinus, with a lower incidence of wound infection and smaller scar, and it is worth recommending to patients with wide wounds, high tension and serious infection. Key words: Pilonidal sinus; Infections diseases; Sacrococcygeal region; Surgical procedures, operative; Vacuum sealing drainage

Effect and safety of medical elastic net bandage on fixing limb venous indwelling needle for newborns

Objective To discuss about the effect and safety of medical elastic net bandage on fixing limb venous indwelling needle for newborns. Methods A total of 120 newborns hospitalized in the pediatric department of this center from September 2012 to December 2015 were selected as study objects.Random number table was used to divide into intervention group and control group (60 cases each).3 M surgical dressing with gauze fixation method was used for the venous indwelling needle of newborns in control group and 3 M transparent surgical dressing with elastic net bandage fixation method was used for the venous indwelling needle of newborns in intervention group.The fixation effect, retention time and adverse reactions of the two groups were compared. Results 54 cases had successful fixation in intervention group, 2 cases had secondary fixation, 3 cases had indwelling needle falling-off and 1 case had transfusion leakage.40 cases of control group had successful fixation, 8 cases had secondary fixation, 7 cases had indwelling needle falling-off and 5 cases had transfusion leakage.Compared with the control group, the fixation success rate of intervention increased obviously, the occurrence rate of secondary fixation, indwelling needle falling-off and transfusion leakage reduced obviously, and the difference had statistical significance(P<0.05). Compared with the control group, the indwelling time of limb venous indwelling needle to the child patients of intervention group was obviously extended.For the part of indwelling needle of child patients in intervention group, 1 case(1.67%) had skin allergy, 1 case(1.67%) had leakage swelling, and the total occurrence rate was 3.34%.For the part of indwelling needle of child patients in control group, 3 cases(5.00%) had skin allergy, 1 case(1.67%) had infection, 2 cases(3.33%) had leakage swelling, and the total occurrence rate was 10.00%.Compared with control group, the total incidence of adverse reactions of children in the intervention group decreased significantly, and the difference was statistically significant(P<0.05). Conclusion Medical elastic mesh bandage used in fixed newborn body can significantly extend the indwelling venous indwelling needle indwelling time, and reduce the incidence of adverse reactions.It is safe and effective, and it is worthy of clinical popularization and application. Key words: Medical elastic net bandage; Newborn; Limb vein indwelling needle; Application effect

Selenium-loaded cellulose film derived from Styela clava tunic accelerates the healing process of cutaneous wounds in streptozotocin-induced diabetic Sprague–Dawley rats

Purpose: Aims of this study is to evaluate the therapeutic effects and toxicity of Se-loaded cellulose film originated from Styela clava tunic (SeSCTF) on cutaneous wounds during diabetic conditions.Materials and methods: Alterations in skin regeneration, angiogenesis and toxicity were examined using streptozotocine (STZ)-induced diabetic Sprague Dawley® (SD) rats with surgical skin wounds after application of SeSCTF for 12 days.Results: SCTF showed high tensile strength (1.64 MPa), low elongation (28.59%), low water vapor transmission rate (WVTR) and outstanding porous structure. Although SeSCTF application did not induce any significant alterations in glucose concentration or toxicity, wound morphology was rapidly recovered in the SeSCTF treated group relative to the gauze (GZ) and SCTF treated group. Moreover, recovery of re-epithelization, wound contraction and number of blood vessel was observed in SeSCTF treated groups when compared with all other groups. Furthermore, the SeSCTF treated group showed complete recovery of key protein expressions of the downstream signaling pathway of vascular endothelial growth factor (VEGF), angiopoietin-2/1 (Ang-2/1), the signaling pathway of insulin receptors and anti-oxidative status.Conclusions: Overall, the results of this study suggest that SeSCTF accelerates the healing process of cutaneous wounds in STZ-induced diabetic SD rats through stimulation of angiogenesis and the glucose receptor signaling pathway.