Eliomys Quercinus Research Articles

Status and distribution of dormice (Mammalia, Gliridae) in Bulgaria

Here we summarize all available data about dormice (Gliridae) in Bulgaria, published one and our own observations, collected in the last 20 years. Four species are found in Bulgaria - the Forest Dormouse (Dryomys nitedula), the Edible Dormouse (Glis glis), the Hazel Dormouse (Muscardinus avellanarius) and the Roach’s Mouse-tailed Dormouse (Myomimus roachi). Altogether we collected 1200 records from 283 locations. A database has been created in which every single record is kept. The most common and widespread species are the Edible (G. glis) and Forest Dormouse (D. nitedula), found in 212 and 139 locations respectively. They inhabit mainly the deciduous and mixed forest in the country. The Hazel Dormouse (M. avellanarius) is found in 77 locations, the bulk of them are in the mountains, but there are some locations along the Danube and southeast Bulgaria. It prefers woods with a well-developed understorey. The Roach’s mouse-tailed dormouse (M. roachi) is the rarest one - found in 26 locations in southeast Bulgaria. In contrast to the other dormice, it avoids woods and lives in semi-open habitats with shrubs, tree lines, and islands in grasslands and agricultural fields. There are several anecdotal reports of the Garden Dormouse (Eliomys quercinus) but no hard proof exists for the presence of this species. We discussed the patterns of distribution, conservation status, and coexistence of Bulgarian dormice.

The contribution of dormice monitoring to the assessment of the conservation status of species in Latvia in accordance with IUCN criteria

In Latvia, four dormouse species have been recorded. In 2016 an annual monitoring of the Hazel Dormouse (Muscardinus avellanarius) and Forest Dormouse (Dryomys nitedula) was commenced within the national biodiversity monitoring programme to obtain data that would allow assessmen of the conservation status of those species and their habitats. The nestbox method was used for the surveys, as it is equally suitable for collecting data on all four species of dormice. Since 2016, 133 temporary study plots with 5–15 nest-boxes each, were established all over the country to record species presence. Sevenadditional permanent study plots collected data on population dynamics, each consisting of at least 50 nestboxes placed within a grid system at 50 m intervals. No Garden Dormice (Eliomys quercinus) were recorded, supporting the presumption that this species has vanished in Latvia. A new location for Edible Dormouse (Glis glis) has been discovered. The Forest Dormouse is found exclusively in a small area near the Latvia-Belarus border, but Hazel Dormice found in Latvia are probably are part of a metapopulation located to the south. No overlapping of distribution areas of species was found. The previous assessment of dormouse species, according to outdated IUCN criteria, was done 20 years ago. Re-assessment according to current IUCN criteria, and using monitoring data, is now carried out within the recently commenced LIFE FOR SPECIES project (2021–2024). Preliminary assessments indicate that each dormouse species will be classified in different categories - rangingfrom Least Concern to Regionally Extinct.

Living in a changing world: Physiological and behavioural flexibility of juvenile Garden Dormice

Heterothermy, or torpor, allows individuals to save energy by active reduction of metabolism and decreased body temperature. Social thermoregulation or huddling allows individuals to minimize energy needs while maintaining a relatively high body temperature. The Garden Dormouse (Eliomys quercinus), a highly endangered European rodent, uses both strategies of torpor and huddling, which are particularly beneficial for young individuals to cope with environmental fluctuations. Since juveniles are an important component of population renewal, determining the flexibility of energy-saving strategies and its consequences on somatic integrity of young individuals is key to protecting this species. We measured individual torpor patterns, body mass gain and structural growth, and assessed telomere dynamics in male and female juvenile Garden Dormice according to housing condition (singly or groups of four individuals) and food availability (fed ad-libitum or energy-limited) before and during hibernation. During development, juveniles used more frequent, longer and deeper torpor when housed singly than in groups. Torpor was encouraged by lower food availability. Juveniles showed similar body mass gain and growth, irrespective of experimental conditions. During hibernation, huddling dormice showed similar hibernating patterns and mass loss compared to single individuals regardless of food availability. Telomere lengthened across all individuals before hibernation, with larger elongation in males, which also experienced greater telomere loss than females during hibernation. We conclude that the use of torpor and huddling allows juvenile Garden Dormice to cope successfully with energetic bottlenecks, without major effects on somatic integrity. Future studies will need to confirm such findings under natural environmental conditions.

Home ranges and activity of Garden Dormice in the Harz Mountains, Germany

For the conservation of endangered species, a good knowledge of their biology is essential. However, due to the secluded lifestyle and nocturnal activity of the Garden Dormouse (Eliomys quercinus), comprehensive research on these small mammals is difficult, resulting in a lack of data. Little is known about the habitat requirements of Garden Dormice in forest habitats. Radio tracking was used to analyze movement patterns to identify specific features of their habitat in the Harz Mountains National Park in Germany (~700 m a.s.l.). Four individuals (2 females, 2 males) were tracked during 11 weeks from May until September 2021. Home ranges were calculated using minimum convex polygon (MCP) and fixed kernel density estimation (KDE) methods. Home range sizes (100% MCP) of males were between 0.58 ha and 7.62 ha and of females between 3.63 ha and 6.39 ha. The size of the core areas (65% KDE) was 0.25 ha - 3.08 ha for males and 1.94 ha - 6.18 ha for females. Whereas the start of their daily activity did not correlate with sunset, the end of activity was associated with sunrise. The females never switched their nesting sites, but the males used 7 -10 nesting sites. Nesting sites were located between boulders and inside deadwood piles. Deadwood is an important habitat for insects which serve as food for Garden Dormice (and other small mammals). To this species in its natural habitat it is necessary to sustain deadwood in forests.

Habitat use and movement of Garden Dormice in the Harz Mountains, Germany

The Garden Dormouse (Eliomys quercinus) is an example of an endangered species with a distribution focus in Germany. Little is known about its habitat use and movements. Radio tracking is a common method used to analyze movement patterns to identify specific habitat requirements. During 11 weeks from May until September 2021, we tracked four individuals (2 females, 2 males) in the Harz Mountains National Park (~700 m a.s.l.). The study area was characterized by a predominance of rocks and spruce. Within one hour, males moved a distance of up to 173.95 m, females covered a maximum distance of 155.62 m. We analyzed whether a hiking trail cutting through the study area represented a potential barrier for the movement of these animals. Three individuals were tracked crossing the path up to 4 times a night. Raspberry bushes along the path were a potential incentive for crossing. A dense rocky cover and a well-developed shrub layer with raspberry bushes within spruce woodlands seem favorable to the presence of Garden Dormice. For the conservation of this species, it is necessary to protect similar habitats. To connect areas with populations of garden dormice, it may be important to encourage bushes with wild fruits among forest edges and pathways.

Nocturnal space and habitat use by Garden Dormice in an urban area in Germany

The Garden Dormouse (Eliomys quercinus) is currently disappearing from parts of its former range. While its population is declining in many parts of Germany, a stable occurrence is found in the city of Wiesbaden. A radio telemetry study was conducted to investigate nocturnal activity and habitat use. Three males and one female were tracked for six weeks from May to July 2021. Males weighed 85 ± 15 g (n = 6), females 63 ± 8 g (n = 2). Home ranges were calculated using the minimum convex polygon (MCP) method. Males had larger home range sizes (MCP100: 2.69 ± 1.8 ha, n = 3) than females (0.53 ha, n = 1). The home ranges of males overlapped. Males regularly crossed roads up to 16 m wide. Animals exhibited nocturnal behavior with sporadic activity at dusk. Garden Dormice preferred structures with more than 75 % cover from a bird's eye view and 50 % cover when viewed from the side. Broad hedges were strongly preferred over single shrubs and trees or structures without vegetation. They were used by the animals for foraging, mating, as a place for other interactions, and as protected pathways through the habitat. The smaller home range sizes compared to previous studies may be due to good food availability in the city. Animals also seem to benefit from continuous habitat and high levels of plant cover. Recommended conservation measures include maintaining and promoting broad hedges and habitat connectivity.

Acoustic detection of Garden Dormice: a field mapping study in an urban habitat in Germany

Garden Dormouse populations have been decreasing in range and declining in number throughout Europe. For effective conservation strategies, it is important to know where and in which habitats the species still occurs. Effective monitoring methods are therefore urgently needed especially because Glirids are difficult to monitor due to their nocturnal activity and their relatively low population density. The city of Wiesbaden (central Germany close to the River Rhine) harbors a stable Garden Dormouse population. In summer 2019 a field mapping study was conducted in which the occurrence of this species was investigated. During the summertime detection of the animals, based on their characteristic vocalizations, was used for the first time to map the occurrence of the species. Within 20 nights we obtained 128 acoustic detections of Garden Dormice, involving at least 168 individuals, and resulting in records for 55 out of 61 km² grids investigated. In Wiesbaden, the species is widespread and could be easily detected by its calls. It was found over almost the whole area, mainly in semi-open areas like gardens and allotments, but also in highly built up and cultivated areas, right in the immediate vicinity of humans, and in some cases even inside buildings. Thus, the Garden Dormouse has successfully adjusted to a life in an urban environment. Using this simple, cheap, fast and non-invasive method can provide many data for biodiversity monitoring and should therefore be given more consideration in future surveys.

Comparing methods to detect Garden Dormice (Eliomys quercinus) in mountainous forest habitats

Garden Dormouse populations show a severe and ongoing decline all over Europe. The drivers for this process are still unknown, as well as the exact distribution of the remaining populations. An evaluation of the occupied habitats could give information on important habitat parameters. Therefore, it is essential to improve detection methods and to evaluate the efficiency of available techniques. In this study, three different methods were tested on ten transects in the Fichtel Mountains, Germany. On each transect we installed two camera traps (CT), one autonomous sound recording unit (AudioMoth, AM) and 25 footprint tunnels (FT), which were checked weekly from the beginning of June until the end of October. AMs did not record a single call, FT detected Garden Dormice in seven and CT detected them in all ten transects. FT worked best during July, when they provided evidence of Garden Dormouse on six transects. CT worked best in August and September, confirming Garden Dormice in all ten transects. In every month, CT outperformed FT in the number of positive findings, and in the time interval until initial detection. CT worked most efficiently in this type of habitat, whereas FT produced false absence data. As Garden Dormice inhabit a variety of different habitats, this study should be repeated to see if the results are transferable.

Number and length of whiskers in dormice

Whiskers (vibrissae) are slender, curved and tapered hairs present in most mammals, which transmit vibrotactile information. In contrast to pelage hair, whiskers are longer and thicker and they possess highly innervated follicles. So called ‘whisking’, a symmetrical, cyclic movement of whiskers can be observed in some tactile specialists. It is used to scan the environment especially during locomotion and foraging. Animals showing the ability of whisking are mainly small, nocturnal and arboreal species like dormice. However, nearly everything we know about whiskers and whisking derives from just a handful of species, including laboratory rats and mice. Until now, information on whiskers and whisking in dormice has been scarce. We describe for the first time the number, length and arrangement of whiskers in the Garden Dormouse, the Edible Dormouse and the Hazel Dormouse. We counted and measured macro- and micro-vibrissae of five cadavers of the three species and calculated median values for each species. Anatomical studies on whiskers provide data for comparative studies between different species. Moreover, knowledge about whiskers and whisker-use can guide us in designing enriched enclosures for captive mammals. This is especially useful for small, nocturnal species that use whiskers for orientation; including a range of textures and climbing frames in their enclosures is important.

Changes in the status of dormice species in Latvia over the last three decades

The first assessment of the status of dormice in Latvia, with emphasis on their distribution, was made in 1993. In accordance with the methodology used within the LIFE-IP LatViaNature project (2020–2028), to set favourable reference values (FRVs) for species range and population, the early 1990s was chosen as the baseline for evaluating population trends of species listed in the Habitats Directive, as this was a socio-economic turning point in the country. The next milestones were 2004 (Latvia joined EU) and the present time. Knowledge about dormice, especially the Forest and Hazel Dormouse, has considerably improved. Since then, one of four dormice species, the Garden Dormouse, has vanished from Latvia. No records of this species are known since the mid-1990s, despite widespread use of nest boxes for monitoring dormice. The limited ranges and population sizes of the Forest Dormouse and Edible Dormouse within the country are related to their occurrence here at their northern limits of distribution. Among other dormice species, the Hazel Dormouse is the most numerous and widespread. Currently, it has three disjunct populations, one of which has probably vanished. Dormice monitoring as part of the national biodiversity monitoring programme started only in 2016. Therefore, long term population trends are unknown. Nest box occupation by the Forest Dormouse has been relatively stable, but in the Hazel Dormouse it decreased in 2019–2021 compared to 2017–2018, most probably, due to mild winters.

Habitats change habits: How Garden Dormice cope with different environments in Germany

The Garden Dormouse is currently disappearing from parts of its range. While its population is declining in many parts of Germany including forest habitats in low mountain ranges, a stable occurrence is found in some cities. A radiotelemetry study was performed in the spruce forests of the Harz National Park (mountains) and the city of Wiesbaden to compare space and habitat use, and the structure and use of daytime nest sites (DNS) used for rest. In total, eight individuals (5 males, 3 females) were tracked between May and September 2021. They used smaller home ranges in the city (MCP100: 2.39 ± 1.34 ha; n = 4) than in the forest (MCP100: 4.56 ± 3.13 ha; n = 4). At night, the animals used structures offering protection from predators and providing food like wide hedges in the city and rock crevices and berry bushes in the forest. The animals were located in a DNS a total of 133 times, and one to ten different DNS per animal were identified. They were situated in structures providing a high degree of protection from predators and also had consistent temperatures (e.g. rock crevices , deadwood piles , hedges, vines on buildings, nest boxes, and buildings). While females rarely changed their DNS, males used several of them. Recommended conservation measures for Garden Dormice include raising public awareness and also the protection and promotion of facade greening, hedges and old buildings in urban areas. A dense rocky cover, as well as a well-developed shrub layer with raspberry bushes are valuable in spruce woodlands .

New palaeontological evidence suggests an early Middle Pleistocene age for the lower levels of Sun Hole Cave, Cheddar, Somerset, UK

Sun Hole, a small fissure cave on the north side of Cheddar Gorge, Somerset, is best known as the site where late Upper Palaeolithic artefacts are associated with human and other mammal remains that immediately pre-date the Lateglacial interstadial. These remains from the upper levels (Unit I) overlie sediments (Unit II) that are thought to have accumulated during a full glacial period, below which are sediments (Unit III) attributed to an interglacial stage. The extinct land snail Retinella (Lyrodiscus) sp., which in Britain was only known from the Hoxnian Stage, had previously been recovered from Unit III. The interglacial at the base of the sequence was therefore assigned to the Hoxnian Stage (Marine Isotope Stage (MIS) 11, 424–374 kyr). Here we provide additional analyses of samples from Unit III, which as well as containing further Retinella (Lyrodiscus), also yielded a specimen of Monachoides incarnatus, its first record from the British Pleistocene. An associated assemblage of small mammals included a few that are rare in the British Pleistocene, such as birch mouse (Sicista cf. betulina) and garden dormouse (Eliomys quercinus). The vole Lasiopodomys gregaloides and the shrew Sorex (Drepanosorex) savini do not occur as late as the Hoxnian and therefore suggest an older age in the early Middle Pleistocene. The record from Sun Hole therefore has parallels with the well-known sequence from the upper Calcareous Member in the nearby cavern system at Westbury-sub-Mendip.

Complete mitogenomes reveal limited genetic variability in the garden dormouse Eliomys quercinus of the Iberian Peninsula

The garden dormouse Eliomys quercinus is a poorly known Western Palearctic species experiencing a global decline. Even though the availability of genetic information is key to assess the driversunderlying demographic changes in wild populations and plan adequate management, data on E. quercinus are still scant. In this study, we reconstructed the complete mitogenomes of four E. quercinus individuals from southern Spain using in–solution enriched libraries, and found evidence of limited genetic variability. We then compared their cytochrome b sequences to those of conspecifics from other countries and supported the divergent but genetically depauperate position of this evolutionarily significant unit (ESU). The information produced will assist future conservation studies on this little–studied rodent.

An hourglass mechanism controls torpor bout length in hibernating garden dormice.

ABSTRACTHibernating mammals drastically lower their rate of oxygen consumption and body temperature (Tb) for several weeks, but regularly rewarm and stay euthermic for brief periods (<30 h). It has been hypothesized that these periodic arousals are driven by the development of a metabolic imbalance during torpor; that is, the accumulation or the depletion of metabolites or the accrual of cellular damage that can be eliminated only in the euthermic state. We obtained oxygen consumption (as a proxy of metabolic rate) and Tb at 7 min intervals over entire torpor–arousal cycles in the garden dormouse (Eliomys quercinus). Torpor bout duration was highly dependent on mean oxygen consumption during the torpor bout. Oxygen consumption during torpor, in turn, was elevated by Tb, which fluctuated only slightly in dormice kept at ∼3–8°C. This corresponds to a well-known effect of higher Tb on shortening torpor bout lengths in hibernators. Arousal duration was independent from prior torpor length, but arousal mean oxygen consumption increased with prior torpor Tb. These results, particularly the effect of torpor oxygen consumption on torpor bout length, point to an hourglass mechanism of torpor control, i.e. the correction of a metabolic imbalance during arousal. This conclusion is in line with previous comparative studies providing evidence for significant interspecific inverse relationships between the duration of torpor bouts and metabolism in torpor. Thus, a simple hourglass mechanism is sufficient to explain torpor/arousal cycles, without the need to involve non-temperature-compensated circadian rhythms.

Distribution modelling of the garden dormouse Eliomys quercinus (Linnaeus, 1766) with novel climate change indicators

The garden dormouse Eliomys quercinus has been declining in both abundance and range since the mid-twentieth century. The eastern edge of its range has contracted from the Ural Mountains to eastern Germany. Habitat loss and fragmentation has been the most supported theory to explain the observed decline. Climate change has been implicated in declines of other terrestrial mammals, but not investigated for E. quercinus. To better understand the factors influencing the distribution of this species and to map habitat suitability for E. quercinus across Europe, we created a Maxent species distribution model. Among the main environmental variables used for the modelling, two novel climate change indicator variables were produced to indicate the degree of climate change between the early twentieth century and the present. Areas of high suitability were mapped, and variable importance estimated using jackknife tests and variable contribution metrics. The climate change indicators outperformed many conventional variables, which could indicate that climate change is a factor behind the current distribution of E. quercinus. We also analysed the land use types where presence points of E. quercinus were located and whether they were in areas of “high nature value farmland”. Over 30% of all spatially filtered presence points corresponded to high nature value farmland areas. Our results could indicate a role for changing climate (particularly in temperature) in the range decline E. quercinus, and for high nature value farmland practices in conserving this species. Field studies and improved monitoring for this species are recommended to confirm both possible findings.

Small vertebrates from a Holocene gravelly deposit at El Salt (Alcoi, Alicante)

The small-vertebrates’ assemblage recovered comes from Units I to IV from El Salt site (Alcoi, Spain). The sample is composed by nearly 310 remains, and includes one toad (Epidalea calamita), two lizards (Lacertidae indet. and Chalcides cf. bedriagai), two snakes (Coronella cf. girondica and cf. Coronella sp.), two insectivores (Crocidura sp. and Sorex sp.), one lagomorph (Oryctolagus cuniculus) and seven rodent taxa (Arvicola sapidus, Microtus sp., M. arvalis, M. cabrerae, M. duodecimcostatus, Apodemus sylvaticus, and Eliomys quercinus). All the species described in the present work are consistent with a Late Pleistocene-Holocene chronology. The presence of M. cabrerae (absent in the Middle Pleistocene) and M. duodecimcostatus (absent in the Mediterranean until the Late Pleistocene) seem to confirm this age. The presence of Neolithic pottery and M. arvalis in the same deposit is inconsistent from a biochronically point of view since this species was extirpated from this region at the end of the Late Pleistocene. Several taphonomic alterations related to transport within the small mammal remains have been identified. Further taphonomical and geoarchaeological work is needed to clarify the provenience of the studied faunal assemblages, which, given the high energy, erosional morphology and lithology of the sedimentary deposit, could have been reworked in a more recent time period.

Dynamic Function and Composition Shift in Circulating Innate Immune Cells in Hibernating Garden Dormice.

Hibernation is characterized by successive torpor bouts during which metabolic rate is down-regulated to 2–4% of euthermic levels along with core body temperatures (Tb) ranging between 0 and 10°C. One characteristic of the torpid state, which is periodically interrupted by a few hours of euthermic phases or arousals during hibernation, resides in an overall impairment of the immune system. The most striking change during torpor is the reduction of circulating white blood cells up to 90%, while their numbers rise to near summer euthermic level upon rewarming. However, potential changes in responsiveness and function of neutrophil granulocytes, accounting for the primary cellular innate immune defense, are unknown. Here we present the first data on shifts in oxidative burst capacity, i.e., the ability to produce reactive oxygen species (ROS), of neutrophils during hibernation. Using a chemiluminescence assay, we measured real-time ROS production in whole blood of hibernating garden dormice (Eliomys quercinus) in early or late torpor, and upon arousals. Accounting for changes in neutrophil numbers along the torpor-arousal cycle, we found significant differences, between torpid and euthermic states, in the neutrophil oxidative burst capacity (NOC), with shallow cell responses during torpor and a highly significant increase by up to 30-fold during arousals. Further, we observed a significant reduction of NOC from aroused animals with euthermic Tb of 36.95 ± 0.37°C, when tested at 6°C, whereas no change occurred in NOC from torpid individuals reaching constant Tb of 4.67 ± 0.42°C, when measured at 35°C. This dynamic indicates that the reduction in NOC during torpor may be temperature-compensated. These results linked to the understanding of immune function during the torpor-arousal cycle might have clinical relevance in the context of therapeutic hypothermia and reperfusion injury.

Erratum to “Palaeoecological context for the extinction of the Neanderthals: A small mammal study of Stratigraphic Unit V of the El Salt site, Alcoi, eastern Spain” [Palaeogeography, Palaeoclimatology, Palaeoecology 530 (2019) 163–175

Abstract El Salt is an important reference site for understanding the extinction of Neanderthal populations in the eastern Iberian Peninsula during MIS 3. In this paper, we describe the small mammal assemblage from Stratigraphic Unit V, the youngest unit with evidence of human presence, based on nearly 1300 specimens. A total of seven rodents (Microtus arvalis, Microtus duodecimcostatus, Microtus cabrerae, Sciurus vulgaris, Arvicola sapidus, Eliomys quercinus and Apodemus sylvaticus), three insectivores (Talpa occidentalis, Crocidura sp., Sorex sp.) and one lagomorph (Oryctolagus cf. cuniculus) were identified. Palaeocological analyses point to drier conditions in this part of the stratigraphic sequence, supporting the hypothesis that an aridification scenario may have played a role in the extinction of the Neanderthal groups inhabiting this region of the Iberian Peninsula.

Rodents Rescue Seeds from Aborted Fruits

Photo 1: Flowering P. bourgaeana trees (upper left), fruiting pear tree (upper right), aborted fruit (lower left), and one aborted and several ripe developed fruits (lower right). Photo credit: Miguel Delibes (upper left), Jose M. Fedriani (all other panels). Photo 2: Some of the rodent species involved in our study concerning P. bourgaeana seed predation and dispersal by rodents; Algerian mouse Mus spretus (upper left), garden dormouse Eliomys quercinus (upper right), wood mouse Apodemus sylvaticus, and Algerian mouse (lower panels). Photo credit: Jose M. Fedriani. Photo 3: Dry aborted fruits (upper line) and fleshy developed fruits (lower line; upper left panel). Dry aborted fruit gnawed by rodents (upper right), and fleshy ripe fruit likely eaten by rabbits Oryctolagus cuniculus (lower left). Fleshy green fruits partially eaten by deer Cervus elaphus when still attached to the tree (lower right). Photo credit: Gemma Calvo. Photo 4: Field experiments to investigate rodent foraging on P. bourgaeana fruits. The “tables” allowed us to prevent fruit consumption by other frugivores such as rabbits, carnivores and ungulates. Photo credit: Gemma Calvo. Photo 5: Other mammals that feed on ripe P. bourgaeana fruits but not aborted ones. Red deer C. elaphus (upper left), wild boar Sus scrofa (upper right), Eurasian badger Meles meles (lower left), and red fox Vulpes vulpes (lower right). Photo credit: Pedro J. Garrote. These photographs illustrate the article “The overlooked benefits of synzoochory: rodents rescue seeds from aborted fruits” by Jose M. Fedriani, Gemma Calvo, Miguel Delibes, Daniel Ayllón, and Pedro J. Garrote published in Ecosphere. https://doi.org/10.1002/ecs2.3298.

Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus.

Differential levels of n-6 and n-3 essential polyunsaturated fatty acids (PUFAs) are incorporated into the hibernator’s diet in the fall season preceding prolonged, multi-days bouts of torpor, known as hibernation. Peroxisome proliferator-activated receptor (PPAR) transcriptional activators bind lipids and regulate genes involved in fatty acid transport, beta-oxidation, ketogenesis, and insulin sensitivity; essential processes for survival during torpor. Thus, the DNA-binding activity of PPARα, PPARδ, PPARγ, as well as the levels of PPARγ coactivator 1α (PGC-1α) and L-fatty acid binding protein (L-FABP) were investigated in the hibernating garden dormouse (Eliomys quercinus). We found that dormice were hibernating in a similar way regardless of the n-6/n-3 PUFA diets fed to the animals during the fattening phase prior to hibernation. Further, metabolic rates and body mass loss during hibernation did not differ between dietary groups, despite marked differences in fatty acid profiles observed in white adipose tissue prior and at mid-hibernation. Overall, maintenance of PPAR DNA-binding activity was observed during torpor, and across three n-6/n-3 ratios, suggesting alternate mechanisms for the prioritization of lipid catabolism during torpor. Additionally, while no change was seen in L-FABP, significantly altered levels of PGC-1α were observed within the white adipose tissue and likely contributes to enhanced lipid metabolism when the diet favors n-6 PUFAs, i.e., high n-6/n-3 ratio, in both the torpid and euthermic state. Altogether, the maintenance of lipid metabolism during torpor makes it likely that consistent activity or levels of the investigated proteins are in aid of this metabolic profile.