Impatiens Balsamina Research Articles

Distinct kin strategies of the legume soybean and the non-legume balsam by accomplishing different nitrogen acquisition and rhizosphere microbiome composition.

SUMMARYKin selection has been proposed vvto be an important mechanism for plant relatives growing together. To reveal kin recognition, we used15N labeling to assess the short‐term nitrogen (N) acquisition (uptake of nitrate and ammonium), long‐term N utilization (recovery of added urea), N‐use efficiency (NUE) and rhizosphere microbiome in leguminousGlycine maxand non‐leguminousImpatiens balsamina. Individuals of each species were planted pairwise with either a sibling or a stranger. Enzyme activity and soil microbial composition were compared between kinship groups. Compared with strangers,G. maxsiblings increased aboveground biomass, NUE, and nitrogenase activity, whereasI. balsaminasiblings decreased root biomass and increased uptake rate of nitrate and potential nitrification rate. Plant kinship affected soil bacterial communities by enriching specific groups possessing explicit eco‐functions (Rhizobiales forG. maxandNitrospiraforI. balsamina). Kinship‐sensitive operational taxonomic units formed independent modules in the bacterial co‐occurrence network and were positively correlated with plant growth performance, N acquisition and enzymatic activity. Plant kin recognition may depend on the growth strategies of the plant species. Kin selection was dominant inG. maxby enhancing biological N fixation through the enrichment of symbiotic rhizobia (demonstrated by aboveground growth and NUE superiority among siblings). Kin selection and niche partitioning occurred simultaneously inI. balsamina, expressed through reduced root allocation but increased nitrate uptake, and enhanced soil N nitrification, by enriching functional microbial groups. Kin recognition responses are the consequence of complex interactions among the host plant, the microbiome, and soil nutrient cycling and utilization processes.

Quick-identification of total phenolic and flavonoid content of Mangifera foetida, Lagerstroemia speciosa and Impatiens balsamina gathered from Riau, Sumatera

Introduction: Variance in a species and climate conditions often impact the phytochemical content and pharmacological properties of many medicinal plants. In this study, we used simple and quick methods to determine the total phenolic and flavonoid contents in ethanol extracts from three species of medicinal plants from Riau, Sumatera. Results obtained were then compared to existing literature. Materials and Methods: Several parts of plant were used, namely Mangifera foetida bark (MB), pericarp (MP), leaves (ML), Lagerstreomia speciosa bark (LB), flower (LFlo), leaves (LL), fruit (LFru), and Impatiens balsamina leaves (IL), root (IR), and flower (IFlo). Total phenolic content was estimated using Folin-Ciocalteau method, while flavonoid content was measured using common colorimetric method. Results and Discussion: Results obtained in this study indicate that LL has the highest flavonoid content (22.116 ± 0.409 mg QE/g) while MB contains the highest phenolic content (24.642 ± 11.087 mg GAE/g) amongst the different samples used in this study. Conclusion: When compared to existing literatures, results presented here indicate that plants from Riau, Sumatera are potential sources of pharmacologic ingredients as indicated by the presence of high flavonoid and phenolic content. Further pharmacological study on specific mechanisms of action from active substances isolated from LL, MB, and IL is required.

Model Agribisnis Impatien Balsamina Sebagai Penggerak Perekonomian Pedesaan dan Pelestarian Budaya Lokal di Bali

Impatien balsamina flower is one of the agricultural products of rural communities as an agribusiness product that has economic and cultural values. The purpose of this study was to determine the profit, profit sharing and marketing margin of Impatien Balsamina agribusiness, to find out the benefits of Impatien Balsamina flowers in preserving local culture in community activities. daily. The impatient model of balsamina agrbinis research was conducted in Sibang Village, Mengwi District, Badung Regency. This study involved 25 respondents consisting of farmers, marketers and consumers of impatien balsamina flowers. The data analysis method used in this research is the analysis of farmer share and marketing margin of impatien balsamina flower. As well as the economic benefits and its role in preserving local culture which is reflected in everyday life. The results showed that the impatien balsamina flower marketing model involved farmers, village collectors, large collectors, retailers and consumers. The highest profit of agribinis impatiens balsamina interest is obtained by agribinis actors in channel III, namely retailers with an average flower sale of Rp 11,500 per kg. The farmer's share of the research shows that the average share received by the farmer is 120% if the farmer sells it to collectors in the village. And the biggest marketing margin is obtained in marketing channel III. Impatien balsamina flowers as a means of ceremony in the local culture of the Balinese people.

Determination of triacylglycerol and fatty acid compositions of Impatiens seed oils using reverse phase high performance liquid chromatography.

In the present work, triacylglycerol and fatty acid compositions of Impatiens balsamina L. and Impatiens walleriana Hook.f. seed oils were determined using reverse phase high performance liquid chromatography with both refractive index and spectrophotometric detections. The presence of conjugated octadecatetraenoic moieties was confirmed by UV and IR spectroscopy. Triacylglycerol (TAG) compositions were performed using an incremental approach and confirmed by the results of MS and electronic spectra. The quantitative analysis of TAG was achieved by careful calibration, introducing correction factors for the sensitivity of each compound. The results showed that both Impatiens seed oils contain the same 23 TAGs. The mole fraction of 15 TAGs containing conjugated moieties was more significant than 88% (for Impatiens balsamina L.) and 81% (for Impatiens walleriana Hook.f.). Seed oils of Impatiens balsamina and Impatiens walleriana contain 43.44% and 36.12% mole of conjugated octadecatetraenoic fatty acids, respectively. These conjugated fatty acids were determined to be α-parinaric (C18:49Z,11E,13E,15Z) and β-parinaric (C18:49Z,11E,13E,15Z), in which isomer β-parinaric represents 23.21% and 26.27% of conjugated octadecatetraenoic acids for I. balsamina and I. walleriana seed oils, respectively. In addition, the mole fraction of α-linolenic acid in both seed oils was also abundant at 24.5% and 28.2% for I. balsamina and I. walleriana. Therefore, I. balsamina L.and I. walleriana seed oils are potential sources of polyunsaturated fatty acids, especially conjugated octadecatetraenoic acids.

Effect of Aquarium Water and Kitchen Waste Water on the Germination and Growth of Brinjal, Chilli, Balsam and Greens

This study was undertaken to analyze a comparative study for the growth of diverse ornamental and vegetative plants such as Brinjal (Solanum melongena), Chilli (Capsicum annum), Balsam (Impatiens balsamina) and Greens (Amaranthus). Based on the utilization of different water alternatives, such as aquarium water and kitchen wastewater, this study ensures to procure an appropriate alternative to tap water that provides plants with healthy growth under adverse conditions of water scarcity. Water is a basic component of plant growth, under harsh conditions of replacing sparkling or fresh water for growing plants, which type of water alternative helps provide the best growth? We examined this study based on the plant growth observation, with a setup each of 3 sets, one set for the control; the other two were assessed for kitchen wastewater and aquarium water accordingly. The period of this examination was for about 40 days. Under the provision of nutrient supplements and a deliberate water quantity of 500 ml, our study was carried out. The growth ratio was observed by the germination rate, leaves count, and the height of the plants.

Podosphaera xanthii causing powdery mildew on Impatiens balsamina in China

Impatiens balsamina (garden balsam) is an annual herb and widely grown for ornamental and medicinal use in China. In 2020 and 2021, powdery mildew symptoms were found on I. balsamina in Xinxiang City, Henan Province, China. The pathogenic fungus was identified based on its morphological characteristics and molecular analysis. The internal transcribed spacer (ITS) region was amplified and sequenced. The sequence obtained for the fungus showed 100% identity with the previously reported Podosphaera xanthii. The virulence of the pathogen on I. balsamina was confirmed by utilizing Koch’s postulates. Therefore, we identified the causal organism of this disease as P. xanthii. This is the first report of powdery mildew caused by P. xanthii on I. balsamina in China. The identification of P. xanthii on I. balsamina will support efforts towards its future control and management.

Quality consistency evaluation of commercial Impatiens Caulis by integrating UPLC-QTOF-MS/MS chemical profiling and UPLC-DAD multi-components quantification approaches

In this study, ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) and ultra-high performance liquid chromatography coupled with photo-diode array spectrometry (UPLC-DAD) were firstly integrated to qualitatively characterize the chemical profiles and quantitatively determine the major components in Impatiens Caulis (IC), a Chinese medicinal herb derived from the dried stem of Impatiens balsamina. The qualitative analysis revealed that there was no difference in chemical profiles of twenty commercial IC samples. A total of 45 components were characterized, there in 5 naphthoquinones, 3 coumarins, 5 phenolic acids, 12 flavonoids and 3 other compounds were identified definitely. However, the quantitative results showed a significant difference in these IC samples. In particular, the highest content of total quantified compounds was even 9.49 times of the lowest one. Furthermore, the average contents of these quantified compounds in twenty commercial IC samples had high values of relative standard deviation (RSD) ranged from 15.64% to 98.76%, suggesting a poor quality consistency in these commercial IC samples. Therefore, efficacy related chemical markers as well as the cultivation region, harvest time and/or post-harvest handling of IC should be further investigated for ensuring its quality and efficacy consistency.

Effects of certain mineral fertilizers on the biological mass of Indigofera Tinctoria and Impatiens Balsamina plants

The article describes the effect of mineral fertilizers on the cultivation of dyed Indigofera (Indigofera tinctoria L.) and henna (Impatiens balsamina L), the amount of their application and the ratio of basic nutrients (nitrogen, phosphorus and potassium) related. It has been established that the adequate development of Indigofera and henna plants depends on the ratio of mineral fertilizers used.

Application of Soil Content Testing on the Growth Media of Water Henna Plants (Impatiens balsamina) with Qualitative Methods

Application of Soil Content Testing on the Growth Media of Water Henna Plants (Impatiens balsamina) with Qualitative Methods

Effects of polystyrene microplastics on the seed germination of herbaceous ornamental plants

Although microplastic (MP) pollution has become an environmental issue worldwide, most related research has been confined to marine ecosystems. The impacts of MPs on terrestrial ecosystems, and especially on terrestrial plants, are poorly studied. In our study, different particle sizes (2 μm and 80 nm) and different concentrations (0, 10, 50, 100, and 500 mg·L−1) of polystyrene MPs were selected as the experimental materials, and their effects on three herbaceous ornamental plants, Trifolium repens, Orychophragmus violaceus, and Impatiens balsamina, were investigated. Seed germination tendency, germination rate, and various physiological and biochemical indicators were observed in the treated plants. The germination rates and germination potentials of these plants decreased significantly as the polystyrene MP concentration increased. Root formation, as well as a decrease in root hair density, was observed. The catalase, superoxide dismutase, hydrogen peroxide, proline, soluble protein, and soluble sugar contents all showed overall trends that increased first and then decreased, which conformed to the “Plant-ES” equation. Thus, polystyrene MPs appeared to have significant inhibitory effects on the seed germination processes of herbaceous ornamental plants.

N,Cl-Codoped Carbon Dots from Impatiens balsamina L. Stems and a Deep Eutectic Solvent and Their Applications for Gram-Positive Bacteria Identification, Antibacterial Activity, Cell Imaging, and ClO- Sensing.

In this study, we first synthesized metal-free N,Cl-doped carbon dots (N,Cl-CDs) using Impatiens balsamina L. stems as green precursors in a deep eutectic solvent (DES). The obtained N,Cl-CDs were characterized through transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, fluorescence (FL) spectroscopy, and ultraviolet (UV) spectroscopy. In addition to the common features of carbon dots (CDs), such as high light stability, small size, low toxicity, good aqueous solubility, and favorable biocompatibility, these N,Cl-CDs exhibited excellent recognition and selectivity for Gram-positive bacteria by doping with N and Cl elements using DES. The N,Cl-CDs with positive charge cannot only differentiate Gram-positive bacteria by selective fluorescence imaging but also have antibacterial effects on Gram-positive bacteria. Through potential, ROS, and morphological analyses of bacteria before and after treatment with N,Cl-CDs, the antibacterial mechanisms of bacteriostasis, Enterococcus faecium, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Salmonella were explored. In addition, N,Cl-CDs demonstrated low cytotoxicity and good cell imaging ability in cancer and normal cells. Moreover, they can be used as a fluorescence sensor for the detection of ClO– with a detection range from 100 nM to 40 μM and a limit of detection (LOD) of 30 nM. In summary, the prepared N,Cl-CDs could be applied as environmentally friendly Gram-positive bacterial identification and antibacterial agents. Additionally, their cell imaging and ClO– detection abilities were outstanding.

Enhanced phytoremediation of Metal(loid)s via spiked ZVI nanoparticles: An urban clean-up strategy with ornamental plants

The increasing industrialization and urbanization are also triggering environmental pollution, mostly unnoticed, in the case of soil pollution due to uncontrolled contamination by toxic elemental dispersion. The present study focused on this aspect and studied the clean-up of urban soil in a low-cost and eco-friendly way to restrict arsenic (As), lead (Pb) and mercury (Hg) contamination. Four potential ornamental plants, Catharanthus roseus (vinca), Cosmos bipinnatus (cosmos), Gomphrena globose (globosa) and Impatiens balsamina (balsamina) were used along with zero valent iron (ZVI) nanoparticles (Fe NPs) for remediation of the soil spiked with As (70 mg kg−1), Pb (600 mg kg−1) and Hg (15 mg kg−1) in a 60 d pot experiment. All plants were divided into four groups viz. control, spiked, spiked+20 mg kg−1 ZVI NP and spiked+50 mg kg−1 ZVI NP. FTIR and SEM were used for ZVI NP characterization. Soil and plant analyses and elemental assessments were done using ICP-MS, XRF and SEM. Among the four plants, cosmos showed the maximum accumulation of toxic elements (41.24 ± 0.022 mg kg−1 As, 139.15 ± 11.2 mg kg−1 Pb and 15.57 ± 0.27 mg kg−1 Hg) at 60 d. The application of ZVI NP at 20 mg kg−1 dosage was found to further augment plants’ potential for metal(loid)s accumulation without negatively hampering their growth. Cosmos were observed to reduce soil As from 81.35 ± 1.34 mg kg−1 to 28.16 ± 1.38 mg kg−1 (65.38%), Pb from 1132.47 ± 4.66 to 516.09 ± 3.15 mg kg−1 (54.42%) and Hg from 17.35 ± 0.88 to 6.65 ± 0.4 mg kg−1 (61.67%) at 60 d in spiked + 20 mg kg−1 ZVI NP treatment. Balsamina was the most sensitive plant and showed the least metal(loid)s accumulation. In conclusion, three of these plants are potent enough to use together for a better and enhanced removal of toxic elements from the contaminated soil with cosmos to be the best amongst these in urban areas.

Potensi Daun Pacar Air (Impatiens balsamina L.) dalam Menghambat Pertumbuhan Jamur Rhizopus oryzae L.

Pacar air (Impatiens balsamina L.) plant belongs to the Balsaminaceae family and is used as an indigenous medicine in Asia for fingernail inflammation, fractures, and rheumatism treatments. Moreover, the antipruritic, antianaphylactic, antifungal, antibacterial, and antitumor activities of some compounds, especially phenolics and quinones from this plant have been studied extensively. Therefore, this study aims to determine the antifungal activity of Pacar air leaves (Impatiens balsamina L.) methanol extract against Rhizopus oryzae L. and the IC50 value. The results showed that an increase in the concentrations of I. balsamina L. leaves methanol extract significantly raised the fungal growth. Furthermore, the extract inhibition against R. oryzae L. at various concentrations of 0.5; 0.7; 0.9; 1.1; and 1.3% w/v were 10, 21.9, 58.06, 68.06, and 79.72%, respectively, while the IC50 value was 0.896%. Hence, the presence of naphthoquinones and other secondary metabolites are responsible for its antifungal activity. Based on these results, the antifungal potential of I. balsamina L. leaves against soft-rot fungi is useful in the future.

Study on the Potential Application of Impatiens balsamina L. Flowers Extract as a Natural Colouring Ingredient in a Pastry Product.

Flowers of the genus Impatiens are classified as edible; however, their inclusion in the human diet is not yet a common practice. Its attractive colours have stirred great interest by the food industry. In this sense, rose (BP) and orange (BO) I. balsamina flowers were nutritionally studied, followed by an in-depth chemical study profile. The non-anthocyanin and anthocyanin profiles of extracts of both flower varieties were also determined by high-performance liquid chromatography coupled to a diode array and mass spectrometry detector (HPLC-DAD-ESI/MS). The results demonstrated that both varieties presented significant amounts of phenolic compounds, having identified nine non-anthocyanin compounds and 14 anthocyanin compounds. BP extract stood out in its bioactive properties (antioxidant and antimicrobial potential) and was selected for incorporation in “bombocas” filling. Its performance as a colouring ingredient was compared with the control formulations (white filling) and with E163 (anthocyanins) colorant. The incorporation of the natural ingredient did not cause changes in the chemical and nutritional composition of the product; and although the colour conferred was lighter than presented by the formulation with E163 (suggesting a more natural aspect), the higher antioxidant activity could meet the expectations of the current high-demand consumer.

Comparative phytoremediation potentials of Impatiens balsamina L. and Crotalaria retusa L. for soil contaminated with used lubricating oil

Soil contaminated with used lubricant oil (ULO) has become an emerging environmental threat. Phytoremediation is a cost effective, environmentally friendly and novel technique with great potential for remediation of soils contaminated with used lubricating oil (ULO). Therefore, the aim of this study was to investigate the phytoremediation potentials of Crotalaria retusa L. and Impatiens balsamina for ULO contaminated soil. A randomized block pot experiment was conducted for each species under plant house conditions in soil contaminated with ULO at a concentration of 5,000 mg kg−1 (0.5% w/w)-30,000 mg kg−1 (3% w/w). The percentage biodegradation of ULO in the rhizosphere soil of both species following 90 days exposure indicated significant (p < 0.05) time dependent increases compared to the respective control carried out in contaminated soil without plants. Comparatively higher biodegradation of ULO was recorded from the rhizosphere of C. retusa L. than that of I. balsamina. The measured total microbial activity and cultivable population size of hydrocarbon utilizing bacteria (HUB) suggest that phytoremediation might have occurred mainly via rhizodegradation. The Allium cepa bioassay revealed the reduction of potential cytotoxicity and genotoxicity with the decrease of ULO in phytoremediated soils. The results further revealed significant (p<0.05) positive correlation of N−NO3−1, extractable P concentration and significant (p < 0.05) negative correlation of soil pH with percentage ULO degradation. Therefore, phytoremediation using the test plant species not only reduced ULO levels, cytotoxicity and genotoxicity but also improved overall soil quality. These results highlight the higher phytoremediation potential of C. retusa than that of I. balsamina for soil contaminated with ULO.

Optimasi formula sampo ekstrak daun pacar air (Impatiens balsamina L.) dengan kombinasi natrium lauril sulfat dan cocamide DEA

Dandruff is an anomalous condition characterized by excessive exfoliation of the horny layer from the scalp. The leaves of the water henna plant (Impatiens balsamina L.) are one of the natural ingredients that have anti-dandruff properties, so they can be used as an active ingredient in making dandruff shampoo. The water henna leaves were extracted using the maceration method with ethanol 96%. Apart from the active ingredients, shampoo also has an important component, namely surfactants. The surfactant used in this study was a combination of sodium lauryl sulfate and cocamide DEA. The purpose of this study was to optimize the surfactant formula of sodium lauryl sulfate and cocamide DEA in water henna leaf extract shampoo. This research was a laboratory experimental research. Formula optimization was done through the Simplex Latice Design (SLD) method approach by making eight formulas. All formulas were evaluated for their physical properties including the foam height test and pH test. The data obtained were processed with Design Expert® software. Based on the physical evaluation of the water henna leaf extract shampoo, the results of the high foam test were 10,025 cm and a pH of 6,12. The results of the acceptability test of shampoo preparations including the parameters of color, smell, texture and viscosity received a fairly good response with the acquisition of values 3,1, 3,3, 3,9 and 4 with information 1 (very dislike), 2 (dislike), 3 (quite like), 4 (like) and 5 (really like). The optimum formula for the shampoo consisting of 5% water henna leaf extract, 1,1863% sodium lauryl sulfate, 19,814% cocamide DEA, 3% CMC, 0,5% citric acid, 0,5% methyl paraben, 0,15% menthol and distilled water had physical properties that meet the requirements of a good shampoo preparation.

GC Analysis of Seven Seed Oils Containing Conjugated Fatty Acids

The fatty acid compositions, including isomer compositions, of seven seed oils containing conjugated fatty acids (CFA) were determined. Seed oils were extracted using a modified Folch extraction, converted to fatty acid methyl esters, and analyzed by gas chromatography (GC) with mass spectrometry and flame ionization detection. The MS detector was operated in positive-ion chemical ionization mode using methane reagent gas. GC was performed using two columns providing different retention characteristics: a poly(ethylene glycol) column and a more polar biscyanopropyl column. The complimentary information provided by the two columns was crucial to peak identification in several cases. The major CFA species in the samples are well known but all contained lesser amounts of additional CFA that have not been widely reported. All samples contained multiple species of conjugated linolenic acid, and two samples also contained small amounts of conjugated linoleic acid. The seed oils of Jacaranda mimosifolia and Calendula officinalis were found to contain 8c,10t,12t-18:3, the natural occurrence of which has only been recently reported in some other samples. The seed oil of Impatiens balsamina has been reported to contain four conjugated 18:4 species, and we present evidence for a fifth conjugated 18:4 isomer.

Flowers and Value of Conservation in The Culture of Hindu Community in Bali

Balinese Hindu community cannot be separated from plant in their life. The information on plant species and how to utilize them are easy to find but limited information about the potency of flowers in the Balinese Hindu community. Flowers have a meaning as sincerity, a symbol of Sang Hyang Widhi, the valour, and also have mythological values. This research aimed to determine the species of flowering plants that are often used for ceremonial and understand the meaning, the cultural conception related to conservation education. The study was carried out in four locations in Tabanan Regency (Baturiti, Penebel, Marga, and Tabanan). Data were collected using an interview with purposive sampling. Data analysis based on the value of Relative Frequency (FR) which is indicated plant most widely used. Â A total of 36 species of flowers are used in traditional Hindu religious ceremonies, of which 29 species are cultivated plants and 7 species are still wild. There are ten of plants which have high FR value, such as: Cananga odorata (FR 0.069), Impatiens balsamina (FR 0.069), Hydrangea macrophylla (FR 0.069), Plumeria acuminata (FR 0.069), Tagetes erecta (FR 0.069), Magnolia champaca (FR 0.069), Gomphrena globosa / bunga ratna (FR 0.047), Gardenia jasminoides / jempiring (FR 0.039), Nymphaea spp./ tunjung (FR 0.030), and Hibiscus rosa-sinensis L./ pucuk (FR 0.030). This research revealed the role of flower in Hindu ceremony and describe the strategic plant conservation due to culture of Balinese Hindu community. Finally, this research expected to documented local knowledge about flowers used as Hindu religious ceremonies, to provide an understanding of the meaning and conceptions of culture related to conservation education.

Growth Responses and Accumulation Characteristics of Three Ornamental Plants to Sn Contamination in Soil

Decorative ornamental plants have been applied as hyperaccumulators/phytoremediators to a wide spectrum of heavy metal contaminants. In this study, pot culture experiments were conducted to investigate the Sn tolerance and accumulation in Impatiens balsamina L., Mirabilis jalapa L. and Tagetes erecta L., in order to assess the possibility of these three ornamental plants to be used as phytoremediators of Sn-contaminated soil. Results show that all three plants exhibited strong tolerance to Sn contamination, and no significant visual toxicity was observed for all three plants grown under most of the Sn treatments. The amount of Sn accumulated in the three plants was positively correlated with the Sn concentration in the soil. The order of the Sn accumulative capacity was Impatiens balsamina &gt; Mirabilis jalapa &gt; Tagetes erecta. Impatiens balsamina and Tagetes erecta showed a low translocation ability (TF) (&lt;1), and the roots accumulated the highest Sn concentration, but Impatiens balsamina showed a relatively high bioconcentration factor (BCF, Sn concentration in each part &gt; 100 mg/kg after Sn treatment of 500 mg/kg). Meanwhile, the TF of Mirabilis jalapa was &gt;1, and the fluorescence accumulated the most Sn. In combination with the adaptation to high concentrations of various heavy metals, these three ornamental plants are potential candidates for Sn mining tailings or contaminated soil.

Multilocus gene analysis reveals the presence of two phytoplasma groups in Impatiens balsamina showing flat stem and phyllody

Rose balsam (Impatiens balsamina) is an important ornamental species grown worldwide for its attractive flowers and also having medicinal properties. Flat stem, little leaf, and phyllody symptoms were observed in I. balsamina nurseries in Uttar Pradesh and Tripura states of India during surveys from 2018 to 2020, with an incidence from 6 to 27%. Amplicons of ~ 1.2 kb were amplified in all the tested symptomatic samples of I. balsamina using universal phytoplasma primer pairs from different surveyed locations, but not from the asymptomatic plants. Pairwise sequence comparison, phylogeny, and virtual RFLP analysis of 16S rRNA gene sequences identified the phytoplasmas as 16SrI-B subgroup strain from Tripura (Lembucherra) and 16SrII-D subgroup strain from Uttar Pradesh (Gorakhpur and Faizabad). Phytoplasma presence and identity was further confirmed by amplifying secA, rp, secY, and tuf genes. This is the first report of 16SrI-B and 16SrII-D phytoplasmas detection in I. balsamina in the world.