Ganoderma Lucidum Spore Research Articles

Effects of sporoderm-broken spores of Ganoderma lucidum on growth performance, antioxidant function and immune response of broilers.

This study was conducted to evaluate the effects of sporoderm-broken spores of Ganoderma lucidum (SSGL), a traditional Chinese medicinal herb, on growth performance, antioxidant ability, and immunity of broilers. Three hundred male broilers with similar body weights (40.0 ± 1.0 g) at 1 d of age were assigned randomly to 4 treatments. Each treatment contained 5 replicates of 15 birds per replicate. The dietary treatments were corn–soybean meal basal diet supplemented with SSGL at the concentrations of 0 (control), 100, 200 and 500 mg/kg diet. The results showed that diets supplemented with SSGL significantly increased (P < 0.05) the average daily gain and decreased (P < 0.05) the feed:gain (F:G) ratio of birds during the finisher period (22 to 44 d of age). Moreover, the total antioxidant capability, glutathione reductase and catalase activities in the liver and spleen were significantly higher (P < 0.05) in broilers fed diets with SSGL than in broilers fed the control diet. Additionally, dietary SSGL also increased (P < 0.05) the serum interleukin (IL)-2, immunoglobulin (Ig) A and IgG levels of broilers compared with the control diet. These results suggest that SSGL have ameliorative effects on growth performance, free radical-scavenging activity, antioxidant capability, and immune function of broilers.

Production of triterpenoid compounds from Ganoderma lucidum spore powder using ultrasound-assisted extraction

When ingested as a dietary supplement, Ganoderma lucidum spore powders (GLSP) provide various health benefits such as enhanced immunity, liver protection and anti-cancer effects. In this study, triterpenoid extraction from GLSP was achieved using an ultrasound-assisted process which was optimized using response surface methodology (RSM). Ultrasound-assisted extraction (UAE) was also compared to the most conventional chemical extraction method. For UAE, optimum extraction conditions were found to be ethanol concentration = 95% v/v; solvent to solid ratio = 50:1 mL/g; ultrasound time = 5.4 min; ultrasound power = 564.7 w, and ultrasound probe distance = 8.2 cm. At optimal UAE conditions, no significant differences were found between experimental (0.97 ± 0.04 %) and predicted values (99%); which indicates appreciable correlation at the 97% confidence interval. The findings show the application of Box–Behnken design (BBD) to predict and optimize triterpenoid yield for UAE of triterpenoid from GLSP. Furthermore, glucose consumption was 2.68 times that of control samples when tested with insulin-resistant HepG2 cell, showing potential use in type 2 diabetes. In addition, triterpenoid extracts show good biocompatibility and inhibition of antioxidant activity.

Optimization conversion of chitosan from Ganoderma lucidum spore powder using ultrasound‐assisted deacetylation: Influence of processing parameters

Chitosan is an extremely valuable polysaccharide and usually obtained from crustacean byproduct. In this work, a new non‐animal source chitosan is obtained from fungal precursors (Ganoderma lucidum spore powders, GLSP) using ultrasound‐assisted deacetylation (USAD), and the effects of processing parameters (irradiation time, solid‐to‐liquid ratio, NaOH concentration, and irradiation power) on the DD (degree of deacetylation) of chitosan were investigated. The highest DD value was obtained at irradiation parameters of 20 min, 10% (g:ml) NaOH, 1:25 (g:ml), and 80 W. In addition, the difference in surface morphology, chemical groups, thermal stability, and crystallinity of the resulting chitosan with the treatment of various irradiation times was examined using the SEM, FTIR, TG, and XRD. The results show a new promising herbal source of edible chitosan and the potential material for biomedical applications. PRACTICAL APPLICATIONS: The chitosan obtained from Ganoderma lucidum spore powders (GLSP) possessed many priorities over the chitosan from shells of crustaceans, which involves a multi‐faceted procedure with several economical and consumer related aspects. In the current work, chitosan is obtained from an herbal source GLSP using ultrasound treatment and the effect of processing parameters on chitosan was investigated. The findings show the development of chitosan from an herbal source with potential in functional food applications.

Ganoderma lucidum spore oil induces apoptosis of breast cancer cells in vitro and in vivo by activating caspase-3 and caspase-9.

The mushroom Ganoderma lucidum (G. lucidum) is a traditional Chinese medicine reported to have a variety of pharmacological properties, including anti-cancer activity. G. lucidum spore oil (GLSO) is a lipid substance extracted from sporoderm-broken spore of G. lucidum. However, the effect of GLSO on breast cancer and the underlying molecular mechanism remain unclear. The aim of this study was to identify the effects of GLSO on breast cancer cells in vitro and in vivo as well as to investigate the mechanistic basis for the anticancer effect of GLSO. First, in vitro MDA-MB-231 cells were treated with GLSO (0.2, 0.4, and 0.6 μL/mL). The protein levels of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), X-linked inhibitor of apoptosis (XIAP), total poly (ADP-ribose) polymerase (PARP), caspase-3 and caspase-8 were examined using western blotting. The mRNA expression levels of Fas-associated protein with death domain (FADD), TNF receptor-associated factor 2 (TRAF2), caspases-3, -8, -9 and Bax were examined using qRT-PCR. Second, in vivo the anticancer properties of GLSO were assessed by H&E, TUNEL and immunohistochemistry in BALB/c mice injected with 4T1 cells. In addition, the levels of caspase-9/caspase-3 signaling pathway proteins in tumor tissue were evaluated by immunoblotting. Finally, MDA-MB-231 cells were treated with caspase inhibitors to measure cell viability, the protein levels were examined with western blotting. The results in vitro showed that GLSO up-regulated the expression of Bax and caspase-3 in MDA-MB-231 cells, but had no effect on the expression of caspase-8. Moreover, the growth of tumors in vivo was significantly suppressed in the GLSO-treated group. The results of Western blot were consistent with in vitro. In vitro, co-treatment of MDA-MB-231 cells with caspase inhibitors reduced the inhibitory effect of GLSO on cell growth. GLSO inhibits the growth of MDA-MB-231 cells and tumors in vivo by inducing apoptosis, which may be achieved through the mitochondrial apoptotic pathway.

Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.) Karst

To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.) Karst. The average molecular weight of this polysaccharide was estimated to be 1.5 × 104 Da. Monosaccharide composition analysis indicated that it was composed solely of glucose. From methylation analysis, 1H and 13C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues. Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice. Furthermore, no drug-related toxic reactions were observed. It was suggested that WGLP might be a potential anti-tumor agent used in the clinic.

Nanolization: Facile Nanolization Strategy for Therapeutic Ganoderma Lucidum Spore Oil to Achieve Enhanced Protection against Radiation‐Induced Heart Disease (Small 36/2019)

Nanolization: Facile Nanolization Strategy for Therapeutic <i>Ganoderma Lucidum Spore Oil</i> to Achieve Enhanced Protection against Radiation‐Induced Heart Disease (Small 36/2019)

Facile Nanolization Strategy for Therapeutic Ganoderma Lucidum Spore Oil to Achieve Enhanced Protection against Radiation-Induced Heart Disease.

Radiotherapy (RT) has been extensively utilized for clinical cancer therapy, however, excessive generation of reactive oxygen species (ROS) is becoming a main cause for radiation-induced heart disease (RIHD). Ganoderma lucidum spore oil (GLSO) is a popular functional food composite with potent antioxidant activity, but it is compromised by poor solubility and stability for further application. Therefore, a strategy for rational fabrication of GLSO@P188/PEG400 nanosystem (NS) is demonstrated in this study to realize good water solubility and achieve enhanced protection against RIHD. As expected, GLSO@P188/PEG400 NS can attenuate X-ray-induced excessive ROS levels thanks to its enhanced free radical scavenging capability, simultaneously protecting on mitochondria from X-ray irradiation (IR). Moreover, GLSO@P188/PEG400 NS alleviates DNA damage and promotes self-repair processes against IR, thus recovering G0/G1 proportion back to normal levels. Furthermore, pre- and post-treated GLSO@P188/PEG400 NS demonstrates potential protection on heart from X-rays in vivo, as evidenced by attenuating cardiac dysfunction and myocardial fibrosis. Meanwhile, the cell antioxidant capacity (including T-SOD, MDA, and GSH-x) stays in balance during this process. This study not only provides a promising strategy for facile nanolization of functional food composites with hydrophobic defects but also sheds light on their cardiac protection and action mechanisms against IR-induced disease.

Supercapacitors with high nitrogen content by cage-like Ganoderma lucidum spore

This paper described one-step method to preparing supercapacitors by using natural ganoderma lucidum spores, whose hollow cage structure could facilitate the transmission and storage of electrons. We have investigated the effects of carbonized temperature and carbonized gas on the electrochemical properties of spores. After carbonizing the spore material under flowing ammonia, the nitrogen doping content of it was 8.86%. The specific capacitance of the prepared electrode was 187.3 F/g (at 1 A/g). After 10,000 charge-discharge cycles, the sample remained 93.3% of its capacity. In addition, after being irradiated by γ ray (50 kGy), the carbonized sample remained 100.7 F/g (at 1 A/g) of specific capacitance.

Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic, antitumor and antioxidant function

A new natural proteoglycan from Ganoderma lucidum spore powder (GLSP) was obtained via solvent extraction and optimized using response surface methodology (RSM). The differences among the characteristics of the new proteoglycan from cracked (proteoglycan-C) and uncracked GLSP (proteoglycan-UC) were explored. The SDS-PAGE results showed the molecular weight of protein contained in proteoglycan-UC (55, 72, 95 kDa) was different to that proteoglycan-C (43, 95 kDa). The differences of these amino acids (Glu, Arg, Leu and Lys) content and monosaccharides (Fuc, Gal, Glc, Man and Gal-AC) content between proteoglycan-C and proteoglycan-UC were determined by HPLC/ ion chromatography. The 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of proteoglycan-C were higher than that proteoglycan-UC. The proteoglycan-UC exhibited stronger hypoglycemic and antibacterial effects against E. coli and S. aureus, and the proteoglycan-C and proteoglycan-UC showed antitumor effects with potential for therapeutic applications.

Engineering of Ganoderma lucidum polysaccharide loaded polyvinyl alcohol nanofibers for biopharmaceutical delivery

Nanofibers comprising dispersed natural bioactive ingredients have received considerable attention due to enhanced pharmaceutical effects. Ganoderma lucidum polysaccharide (GLP) is a versatile bioactive compound that is prone to oxidative damage. In this work, GLP was obtained from Ganoderma lucidum spore powder (GLSP) using two methods; ultrasound-assisted extraction (UAE) and traditional solvent extraction (TSE). Optimal GLP yield was determined via parametric optimization for UAE. GLP was subsequently loaded (via electrospinning) as a molecularly dispersed system into polyvinyl alcohol (PVA) nanofibers; which ranged in size from 390 to 750 nm. Composite fibers were analyzed using electron microscopy, Fourier Transform Infrared spectroscopy, and their surface hydrophilicity and mechanical properties were assessed. The results showed that the yield of GLP using UAE for 3 min was higher than that of TSE for 5 h. GLP concentration was shown to influence mean fiber diameter, water contact angle and maximum tensile/strain strength of fibrous composite films. All fibrous films exhibited rapid GLP release, reaching 100% within 300 s. These results demonstrate an efficient method for GLP preparation and a feasible route to encapsulate the compound as a molecularly dispersed system into nanofibers, elucidating potential application in biopharmaceutical delivery.

Microcapsulation of Ganoderma Lucidum spores oil: Evaluation of its fatty acids composition and enhancement of oxidative stability

Ganoderma lucidum (G. lucidum) spores oil, which was extracted from G. lucidum spores with supercritical fluid CO2, contains a lot of unsaturated fatty acids (PUFAS). PUFAS in G. lucidum spores oil were oxidized easily, so it is important to protect G. lucidum spores oil and enhance its oxidation stability. In this paper, we investigated a way of protecting G. lucidum spores oil by microcapsulation with spray drying. Different combination types of wall materials were evaluated and the best choose was the gum Arabic (GA) and maltodextrin (MD) combination. Then, the effects of the emulsifying temperature, the wall materials concentration, and the ratio of oil to wall materials on the GA/MD microcapsules were investigated and the encapsulation efficiency (EE) was up to 83.57%. Gas chromatography was used to identify the components of fatty acids in original and GA/MD microcapsules. It showed that PUFAS were well retained and the content of it was up to 80.04%. Oxidation stability of G. lucidum spores oil was enhanced significantly. All results indicated that using GA and MD as composite wall materials could effectively protect for G. lucidum spores oil.

Researches and Application of Ganoderma Spores Powder.

Ganoderma lucidum spores(GLS) are the mature germ cells of Ganoderma lucidum. They have all the genetic substances and similar active components of Ganoderma lucidum. Similar to the fruiting body of Ganoderma lucidum, ganoderma spores powder has the effect of regulating immunity, antitumor, antioxidation, and protecting cells and so on. In recent decades, with the development of the technology of breaking the wall of Ganoderma lucidum spores and the technology of extracting and preparing, the researches and application of Ganoderma lucidum spores powder have made great progress.

Anti-breast Cancer Enhancement of a Polysaccharide From Spore of Ganoderma lucidum With Paclitaxel: Suppression on Tumor Metabolism With Gut Microbiota Reshaping.

Increasing evidence highlights the cardinal role of gut microbiota in tumorigenesis and chemotherapy outcomes. Paclitaxel (PTX), although as a first-line chemotherapy reagent for breast cancer, still requires for improvement on its efficacy and safety due to drug resistance and adverse effects. The present work explored the enhancement of a polysaccharide derived from spore of Ganoderma lucidum (SGP) with PTX in a murine 4T1-breast cancer model. Results showed that the combination of PTX and SGP displayed an improved tumor control, in which mRNA expression of several Warburg effect-related proteins, i.e., glucose transporter 3 (Glut3), lactate dehydrogenase A (Ldha), and pyruvate dehydrogenase kinase (Pdk), and the metabolite profile of tumor was evidently altered. Flowcytometry analysis revealed that the combination treatment recovered the exhausted tumor infiltration lymphocytes (TILs) via inhibiting the expressions of immune checkpoints (PD-1 and Tim-3), while PTX alone evidently increased that of CTLA-4. 16S rRNA sequencing revealed a restoration by the combination treatment on gut microbiota dysbiosis induced by PTX, especially that Bacteroides, Ruminococcus, and other 5 genera were significantly enriched while the cancer-risk genera, Desulfovibrio and Odoribacter, were decreased. Moreover, spearman correlation analysis showed that abundance of Ruminococcus was significantly negative-associated with the amount of frucotose-6-phosphate within the tumor. Collectively, the present study suggests the clinical implication of SGP as an adjuvant candidate for PTX against breast cancer, which possibly relies on the regulation of tumor metabolism and gut microbiota.

Synthesis and Evaluation of Herbal Chitosan from Ganoderma Lucidum Spore Powder for Biomedical Applications

Chitosan is an extremely valuable biopolymer and is usually obtained as a byproduct from the shells of crustaceans. In the current work, chitosan is obtained from an herbal source (Ganoderma lucidum spore powder (GLSP)) for the first time. To show this, both standard (thermochemical deacetylation, (TCD)) and emerging (ultrasound-assisted deacetylation (USAD)) methods of chitosan preparation were used. The obtained chitosan was characterized by elemental analysis, XRD (X-ray diffraction), FT-IR (Fourier transform infrared spectroscopy) and thermogravimetric measurements. The process resulted in chitosan possessing comparable values of DD, [η] and ,overline{{rm{Mv}}} to the commercial product. Chitosan obtained via both processes (TCD and USAD) displayed excellent biocompatibility; although the USAD prepared biopolymer exhibited significantly improved fibroblast (L929 cell) viability and enhanced antibacterial zones for both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The findings of new herbal chitosan mark key developments of natural biomaterials; marking a potential shift from conventional sea-based organisms.

Ultrasound-assisted synthesis of chitosan from fungal precursors for biomedical applications

Chitin, from the fungal source (Ganoderma lucidum spore powders, GLSP), was converted into chitosan via dual-frequency ultrasound irradiation which produced enhanced results compared with single-frequency processing. The differences between the effects of dual-frequency ultrasound irradiation (at 15 kHz and 20 kHz) with two superposition modes, in orthogonal and parallel orientations, were studied. SEM images confirmed morphology change in the presence of the dual-frequency sources. The enhancement of the degree of deacetylation (DD), dynamic viscosity ([η]) and molecular weight (Mv¯) of the resultant chitosan were also improved by the orthogonal configuration for dual-frequency ultrasound irradiation. In addition, the FTIR, TGA, XRD and 13C NMR results show the differences in chemical groups, thermal stability and crystalline using two different ultrasound conditions in detail. The resulting biocompatible sample improved the proliferation of L929 cells, while antibacterial activity was also observed using E. coli and S. aureus. This presents a promising new use of a fungal material for biomedical applications.

Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling.

As breast cancer is the leading cause of cancer-related deaths in women population worldwide, ongoing endeavor has been made for alternative regimens with improved efficacy but fewer adverse effects. Recently, active components from the spores of Ganoderma lucidum have attracted much attention for their versatile biological activities owing to the advance in sporoderm-breaking technology. Here, anticancer potential of an extract derived from the sporoderm-breaking spores of G. lucidum (ESG) was explored in a 4T1-breast cancer xenograft mice model. Results showed that ESG was able to suppress 4T1 tumor growth in vivo rather than in vitro. Flowcytometry analysis revealed that ESG could significantly increase both cytotoxic T cell (Tc) population and the ratio of Tc to helper T cell (Th) in peripheral blood of the tumor-bearing mouse; similar promotion on Tc was also found in tumor-infiltrating lymphocyte. Moreover, ESG evidently downregulated the two immune checkpoints, programmed cell death protein-1 (PD-1, in the spleen) and cytotoxic T lymphocyte antigen-4 (CTLA-4, in the tumor), suggesting that ESG could effectively restore the T cell paradigm by recovering the exhaustion status via suppressing the co-inhibitory checkpoints. By 16S rRNA gene sequence analysis on the fecal microbiota, it was found that ESG would remodeling the overall structure of the samples from tumor-bearing mice toward that of the normal counterparts, including 18 genera in 5 phyla, together with regulations on several genes that are responsible for signaling pathways involved in metabolism, cellular processes, and environmental information processing. Collectively, this study demonstrated that ESG would serve as a natural anticancer adjuvant via a restoration on the exhausted Tc, highlighting important clinical implications for the treatment of breast cancer.

Supercritical fluid extraction effectively removes phthalate plasticizers in spores of Ganoderma lucidum.

Phthalate plasticizers residue in food is a serious threat to public health. Spores of Ganoderma lucidum are easy to be contaminated with phthalates during collection and processing. In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated. The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum. No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores. However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores. Accelerated oxidation tests further implied that SFE could improve the stability of spores' oil. Our results suggested SFE is a potential approach to remove phthalate from food related products.

A multi-center survey on dietary knowledge and behavior among inpatients in oncology department.

To investigate the current associated factors of dietary knowledge and behavior, the intake and nutritional status in malignancy Chinese inpatients, and the malnutrition causes involved in dietary nutrition knowledge level and behavior, providing recommendations to patients for nutrition education and intervention. Five hundred and thirty-five participants from 18 hospitals were investigated by a questionnaire related to dietary knowledge and behavior. Physicians asked and recorded the level of dietary intake and appetite scoring of the participants. The nutritional risk screening with the Nutritional Risk Screening 2002 (NRS-2002) and the dietary survey by 24h dietary recalls were completed by a dietitian. Besides, the target energy intake and the target protein intake were calculated by the "rule of thumb" recommended by ESPEN guideline, comparing the difference between the actual intake and target intake. According to the questionnaire, 95.2% of participants thought it was important to have a good dietetic habit, and nearly half of them have searched for guides on how to diet; 70% of the patients had no clear idea of what was a scientific diet; 82% of patients had contradictory dietary knowledge; 64.2% of patients would listen to the opinion of the attending physician when a contradiction happened. The main three ways of learning about healthy diet were attending physician, network, and TV, respectively, with the values 26.0, 18.5, and 16.1%. Importantly, 99.6% of patients have made mistakes about dietary knowledge, for example, crab, chicken, lamb, fish, and prawns should not be eaten in their concept. In addition, more than 90% of participants have taken Ganoderma lucidum spore powder, sea cucumber, ginseng, Cordyceps sinensis, and so on. Ninety-three percent of the patients never reached a qualified nutrition education. Besides, 15.6% of the participants had nutritional risk (NRS-2002 ≥ 3). The actual daily energy intake was 1169.20 ± 465.97kcal, which was significantly less than target energy intake (P < 0.01), amounting to 65.3% of the target requirements. The actual daily protein intake was 46.55 ± 21.40g, which was significantly less than target protein intake (P < 0.01), amounting to 74.44%. On the other hand, 69% of the participants were "Not too bad, Ok, Good, or Very good" according to the records of physicians, while 34% of them did not reach 60%of the target requirements through dietary survey. The survey indicated that cancer patients had poor understanding of the scientific dietary nutrition and were in low level of normative nutritional education among Chinese malignancy inpatients. Dietary intake of most cancer patients decreased, and the actual intake cannot be revealed by NRS-2002 score or the physicians' inquiry. It is necessary to enhance the cooperation between dietitians and physicians and develop nutrition education to improve the level of dietary knowledge.

Designer fibers from 2D to 3D – Simultaneous and controlled engineering of morphology, shape and size

Topography and surface morphology of micrometer and nanometer scaled fibrous biomaterials are crucial for bioactive component encapsulation, release, promoting cell proliferation and interaction within biological environment. Specifically, for drug delivery and tissue repair applications, surface engineering provides control on both aspects in tandem. In this study, the bioactive component (ganoderma lucidum spore polysaccharide (GLSP)) was loaded into zein prolamine (ZP) fiber matrices via coaxial electrospinning (CES) technique. During the CES process, various outer layer enveloping fluids were used to modulate fiber topography in-situ (from 2D to 3D). SEM and water contact angle tests indicate enveloping media impact electrospun fiber diameter (ranging from 400nm to 3.0μm) and morphologies (from flat ribbon-like to solid cylindrical structures), with the latter impacting GLSP release profile. Furthermore, CCK-8 assay assessment indicates fibroblast cell proliferation (L929 cell line), while cell extension was also observed for modified ZP fibers. The results demonstrate potential applications of modified fiber morphologies, which are tailored in-situ without impacting chemical stability and encapsulation.

The ethanol extracts of sporoderm-broken spores of Ganoderma lucidum inhibit colorectal cancer in vitro and in vivo.

The medicinal mushroom Ganoderma lucidum (G. lucidum) has been reported to possess a variety of pharmacological activities including anticancer effects. However, the anti-colorectal cancer effects and the potential molecular mechanisms of the ethanol extracts of sporoderm-broken spores of G. lucidum (BSGLEE), which mainly contains triterpenoids, have not been reported. The aim of the present study was to investigate the anticancer effects and molecular mechanisms exerted by BSGLEE on colorectal cancer in vitro and in vivo. MTT assay revealed that BSGLEE at 1.6 to 10 mg/ml significantly inhibited HCT116 cell proliferation in a dose- and time-dependent manner. Flow cytometric analysis demonstrated that BSGLEE induces apoptosis and cell cycle arrest at G0/G1 phase, which are associated with deregulation of the expression of key genes and proteins (p21, p16, cyclin D1, Bcl-2, bax, NAG-1, PARP and caspase-3) that regulate apoptosis and cell cycle cascades. Moreover, BSGLEE significantly inhibited HCT116 cell migration via downregulating MMP-1, MMP-2 and upregulating E-cadherin expression at mRNA levels. Oral gavage of 75 and 150 mg/kg BSGLEE significantly inhibited HCT116 xenograft tumor growth in nude mice, which was accompanied by suppressed Ki-67 staining as determined by immunochemistry. Collectively, we found that BSGLEE effectively inhibits colorectal cancer carcinogenesis through induction of apoptosis, inhibition of migration and promotion of cell cycle arrest. Our results suggest that triterpenoids of sporoderm-broken spores of G. lucidum ethanol extracts may serve as a promising anticancer agent for colorectal cancer chemoprevention and therapy.