Functional Role Research Articles

Curved multiplanar reformatting allows the accurate histological delineation of hippocampal subfields.

Hippocampal subfields perform specific roles in normal cognitive functioning and have distinct vulnerabilities in neurological disorders. However, measurement of subfields with MRI is technically difficult in the head and tail of the hippocampus. Recent studies have utilized curved multiplanar reconstruction (CMPR) to improve subfield visualization in the head and tail, but this method has not yet been applied to histological data. We utilized BigBrain data, an open-source database of serially sectioned histological data for our analyses. The left hippocampus was segmented according to histological criteria by two raters in order to evaluate intra- and inter-rater reliability of histology-based segmentation throughout the long axis. Segmentation according to our previous protocol for the hippocampal body was then compared to these histological measurements to evaluate for histological validity. Agreement between segmentations was evaluated using Dice similarity coefficients (DSCs). Intra-rater reliability (DSCs) of histological segmentation was excellent for all subfields: CA1 (0.8599), CA2 (0.7586), CA3/CA4/DG (0.8907), SLM (0.9123), subiculum (0.8149). Similarly, inter-rater reliability analysis demonstrated excellent agreement (DSCs) for all subfield locations: CA1 (0.8203), CA2 (0.7253), CA3/CA4/DG (0.8439), SLM (0.8700), subiculum (0.7794). Finally, histological accuracy (DSCs) for our previous protocol was excellent for all subfields: CA1 (0.8821), CA2 (0.8810), CA3/CA4/DG (0.9802), SLM (0.9879), subiculum (0.8774). When subfields in the hippocampus head, body, and tail were analyzed independently, DSCs also showed excellent agreement. CMPR allows reliable subfield segmentation based on histological criteria throughout the hippocampal head, body, and tail. Our previous protocol for the hippocampal body can be applied to provide histologically valid subfield measurements throughout the entire hippocampal long axis.

Mediating Role of Social Support and Family Functioning on Quality of Life of Family Caregivers of Individuals With Alcohol Use Disorder

Background: Caring for persons with alcohol use disorder (AUD) significantly affects the quality of life (QoL) of caregivers. According to Lazarus and Folkman’s Stress and Coping Model (1984), the severity of alcohol consumption (SAC) is a major stressor for AUD caregivers. These stressors impact well-being, with QoL linked to caregivers’ coping resources, especially social support (SS) and family functioning (FF). The study aimed to investigate the mediating roles of SS and FF in the relationship between the SAC and caregivers’ QoL. Material and Methods: A cross-sectional study was conducted in two psychiatric hospitals in Amritsar city, Punjab. We used a purposive sampling technique to collect data from 128 family caregivers aged 18 and above who were caring for male patients with AUD aged between 20 and 65. The assessment tools used were the World Health Organization Quality of Life-Brief Version (WHOQOL-BREF), Alcohol Use Disorders Identification Test (AUDIT), Family Assessment Device (FAD), and PGI Social Support Questionnaire (PGI SSQ). Results: There is a significant and partial mediation of the association between SAC and QoL by both SS and FF ( P < .001, 99% CI). Moreover, SAC (r = –0.519), FF (r = –0.603), and SS (r = 0.641) showed significant correlations with caregivers’ QoL ( P < .001). The mean scores for SAC, SS, FF, and QoL were 22.66 (11.38), 50.88 (9.45), 127.18 (43.85), and 84.13 (18.70), respectively, suggesting moderate SAC in patients, moderate to high perceived SS levels, moderate perception of family dysfunction, and moderate to high QoL among the caregivers. Conclusion: SAC detrimentally impacts caregivers’ QoL both directly and indirectly through SS and FF, with the latter serving as mediators, partially mitigating SAC’s negative impact. Clinical implications underscore the importance of tailored interventions, emphasizing the strengthening of support systems and consideration of diverse FF domains for personalized approaches. The findings contribute valuable insights for developing targeted interventions customized to the specific needs of AUD caregivers to enhance their overall QoL.

Vegetable Mountain Products, as Functional Food. A Cross-sectional Study of European Romanian Agricultural Producers

Background:: Mountain areas, in a normal ecosystemic context as the analyzed region from the European Romanian Carpathians, offer healthier solutions through different agronomical practices and solutions designed for the production of functional food. The paper approaches a chain formed by mountain functional food – agronomic practices – mountain products commerce, the purpose being the development of the entire mountain products value chain from an area focusing on the matrix "from the farm to the fork". The paper analyzes highly consumed mountain products with functional food roles Allium cepa, Allium sativum, Cucumis sativus, Capsicum, and Solanum lycopersicum. Objective: The research highlights the importance of vegetable mountain products as a functional food in the current hunger and environment contexts, in a more and more polluted world Methods:: The experimental, clinical, and agronomical research, together with the production territorial profile, show that mountain products present high qualitative valences comparatively with low-land areas or with reference values given by USDA. Results:: According to macro-nutritional and micro-nutritional analysis – lipids-fats, saturated fatty acids, protein, cyanocobalamin (B12), ergocalciferol (D2 ), cholecalciferol (D3 ), iron and calcium – Allium sativum dominates the mountain product's top with a functional food role. Instead of this, mountain producers prefer to cultivate mountain products with a higher income horizon. Conclusion:: Functional food represents an imperative in a more polluted world. Being less polluted than other ecosystems, the mountain area offers healthier agricultural products and requires notable investments, together with more involved agronomy.

Gene expression analysis of microRNA-1285 in the South Indian oral squamous cell carcinoma population.

Oral squamous cell carcinoma (OSCC) is the most common oral malignant tumor, which has poor prognosis. The traditional investigative modality is invasive biopsy which is the gold standard for diagnosis. In recent years, alternative methods like non-invasive biomarkers have been studied for their potential role in early diagnosis and prognosis. Among them, microRNAs (miRNAs or miRs) are short non-coding RNAs that regulate gene expression in various diseases, including OSCC. Several miRNAs are being researched as non-invasive biomarkers as well as novel therapeutic targets in the treatment of OSCC. MiR expression can be upregulated or downregulated in OSCC. Among the reported miRNAs, miR-1285 is an important miRNA found to be involved in OSCC. The aim of the current study was to quantify the levels of miR-1285 in OSCC samples and to validate their potential role as biomarkers for OSCC detection. Sixteen samples of cancer tissue and normal tissue were evaluated from a total of 25 patients, in the study, conducted in the Department of Oral and Maxillofacial Surgery. The tissues were processed for H&E staining and gene expression analysis of miR-1285. The samples were collected after proper informed consent from the patients. Total RNA isolated was reverse transcribed into cDNA which was used in the gene expression analysis using qRT-PCR. The histopathological examination confirmed the OSCC cases and the gene expression analysis revealed that miR-1285 was significantly downregulated in OSCC tissues. Since miR-1285 showed significant difference between the OSCC and normal tissues it could be postulated as a potential biomarker and therapeutic target for OSCC. Further in-vitro and in-vivo studies could validate their functional role in OSCC.

Effects of Mesozooplankton Growth and Reproduction on Plankton and Organic Carbon Dynamics in a Marine Biogeochemical Model

AbstractMarine mesozooplankton play an important role for marine ecosystem functioning and global biogeochemical cycles. Their size structure, varying spatially and temporally, heavily impacts biogeochemical processes and ecosystem services. Mesozooplankton exhibit size changes throughout their life cycle, affecting metabolic rates and functional traits. Despite this variability, many models oversimplify mesozooplankton as a single, unchanging size class, potentially biasing carbon flux estimates. Here, we include mesozooplankton ontogenetic growth and reproduction into a 3‐dimensional global ocean biogeochemical model, PISCES‐MOG, and investigate the subsequent effects on simulated mesozooplankton phenology, plankton distribution, and organic carbon export. Utilizing an ensemble of statistical predictive models calibrated with a global set of observations, we generated monthly climatologies of mesozooplankton biomass to evaluate the simulations of PISCES‐MOG. Our analyses reveal that the model and observation‐based biomass distributions are consistent ( = 0.40, total epipelagic biomass: 137 TgC from observations vs. 232 TgC in the model), with similar seasonality (later bloom as latitude increases poleward). Including ontogenetic growth in the model induced cohort dynamics and variable seasonal dynamics across mesozooplankton size classes and altered the relative contribution of carbon cycling pathways. Younger and smaller mesozooplankton transitioned to microzooplankton in PISCES‐MOG, resulting in a change in particle size distribution, characterized by a decrease in large particulate organic carbon (POC) and an increase in small POC generation. Consequently, carbon export from the surface was reduced by 10%. This study underscores the importance of accounting for ontogenetic growth and reproduction in models, highlighting the interconnectedness between mesozooplankton size, phenology, and their effects on marine carbon cycling.

Reflective functioning mediates the relationship between insecure adult attachment and the severity of prolonged grief symptoms.

This study aimed to investigate the mediation role of reflective functioning (i.e., certainty and uncertainty about mental states) in the relationship between insecure attachment (i.e., attachment avoidance and attachment anxiety ) and the severity of prolonged grief symptoms. A cross-sectional study was conducted with 329 bereaved participants (51.7% females, mean age= 46.94 ± 14.62 years). Participants completed the Prolonged Grief Scale (PG-13), the Attachment Style Questionnaire (ASQ), and the Reflective Functioning Questionnaire (RFQ). Demographic and bereavement-related information were also collected. Certainty about mental states fully mediated the relationship between both the attachment avoidance and attachement anxiety and severity of prolonged grief symptoms. The findings of this study indicate that reflective functioning impairment, specifically certainty about mental states of self and others, mediate the paths from insecure attachment to prolonged grief symptoms. This suggests that improving reflective functioning may contribute to the treatment bereaved individuals at risk of PGD.

Signatures of Mollicutes-related endobacteria in publicly available Mucoromycota genomes.

Mucoromycota fungi and their Mollicutes-related endobacteria (MRE) are an ideal system for studying bacterial-fungal interactions and evolution due to the long-term and intimate nature of their interactions. However, methods for detecting MRE face specific challenges due to the poor representation of MRE in sequencing databases coupled with the high sequence divergence of their genomes, making traditional similarity searches unreliable. This has precluded estimations on the diversity of MRE associated with Mucoromycota. To determine the prevalence of previously undetected MRE in fungal genome sequences, we scanned 389 Mucoromycota genome assemblies available from the National Center for Biotechnology Information for the presence of MRE sequences using publicly available tools to map contigs from fungal assemblies to publicly available MRE genomes. We demonstrate a higher diversity of MRE genomes than previously described in Mucoromycota and a lack of cophylogeny between MRE and the majority of their fungal hosts. This supports the late invasion hypothesis regarding MRE acquisition across most of the examined fungal families. In contrast with other Mucoromycota lineages, MRE from the Gigasporaceae displayed some degree of cophylogeny with their hosts, which may indicate that horizontal transmission is restricted between members of this family or that transmission is strictly vertical. These results underscore the need for a refined process to capture sequencing data from potential fungal endosymbionts to discern their evolution and transmission. Screens of fungal genomes for MRE can help improve the quality of fungal genome assemblies while identifying new MRE lineages to further test hypotheses on their origin and evolution.IMPORTANCEMollicutes-related endobacteria (MRE) are obligate intracellular bacteria found within Mucoromycota fungi. Despite their frequent detection, MRE roles in host functioning are still unknown. Comparative genomic investigations can improve our understanding of the impact of MRE on their fungal hosts by identifying similarities and differences in MRE genome evolution. However, MRE genomes have only been assembled from a small fraction of Mucoromycota hosts. Here, we demonstrate that MRE can be present yet undetected in publicly available Mucoromycota genome assemblies. We use these newfound sequences to assess the broader diversity of MRE and their phylogenetic relationships with respect to their hosts. We demonstrate that publicly available tools can be used to extract novel MRE sequences from assembled fungal genomes leading to insights on MRE evolution. This work contributes to a greater understanding of the fungal microbiome, which is crucial to improving knowledge on the dynamics and impacts of fungi in microbial ecosystems.

Examining the role of personality functioning in a hierarchical taxonomy of psychopathology using two years of ambulatory assessed data

The Hierarchical Taxonomy of Psychopathology (HiTOP) arranges phenotypes of mental disorders based on empirical covariation, ranging from narrowly defined symptoms to higher-order spectra of psychopathology. Since the introduction of personality functioning (PF) in DSM-5 and ICD-11, several studies have identified PF as a predictor of transdiagnostic aspects of psychopathology. However, the role of PF in the HiTOP classification system has not been systematically examined. This study investigates how PF can be integrated into HiTOP, whether PF accounts for transdiagnostic variance captured in higher-order spectra, and how its predictive value for future affective well-being (AWB) and psychosocial impairment (PSI) compares to the predictive value of specific psychopathology beyond PF. To this end, we examined two years of ambulatory assessed data on psychopathology, PF, PSI, and AWB of N = 27,173 users of a mental health app. Results of bass-ackwards analyses largely aligned with the current HiTOP working model. Using bifactor modeling, aspects of PF were identified to capture most of the internalizing, thought disorder, and externalizing higher-order factor variance. In longitudinal prediction analyses employing bifactor-(S-1) modeling, PF explained 58.6% and 30.6% of variance in PSI and AWB when assessed across one year, respectively, and 33.1% and 23.2% of variance when assessed across two years. Results indicate that personality functioning may largely account for transdiagnostic variance captured in the higher-order components in HiTOP as well as longitudinal outcomes of PSI and AWB. Clinicians and their patients may benefit from assessing PF aspects such as identity problems or internal relationship models in a broad range of mental disorders. Further, incorporating measures of PF may advance research in biological psychiatry by providing empirically sound phenotypes.

The mediating role of family functioning between childhood trauma and depression severity in major depressive disorder and bipolar disorder

BackgroundChildhood trauma (CT) and family functioning exert significant influences on the course and long-term outcome of major depressive disorder (MDD) and bipolar disorder (BD) patients. Hence, we examined the intricate relationship between CT, family function, and the severity of depressive episodes in MDD and BD patients. Methods562 patients with depressive episodes (336 MDD and 226 BD) and 204 healthy controls (HCs) were included in this retrospective study. The 17-item Hamilton Depression Rating Scale (HAMD-17), Childhood Trauma Questionnaire (CTQ), and Family Adaptability and Cohesion Evaluation Scale (FACES II-CV) were assessed. Pearson correlation analysis and mediation analysis were performed. ResultsCT had both a direct and indirect impact on depression severity in MDD and BD groups. In MDD, family adaptability mediated the impact of all CT subtypes on depression severity (Effect = 0.113, [0.030, 0.208]). In BD, family cohesion played a mediating role between emotional neglect (EN) and HAMD-17 scores (Effect = 0.169, [0.008, 0.344]). Notable differences were observed in onset age, illness duration, episode frequency, family history, and CT subtypes between MDD and BD (P < 0.05). LimitationsThis study has several limitations including recall bias, lack of objective family functioning measures, small sample size, and cross-sectional design. ConclusionsFamily functioning mediated the impact of CT on depressive symptoms severity in MDD and BD patients. MDD patients with a history of CT exhibited reduced family adaptability, while BD patients with a history of EN had weaker familial emotional bonds. Our findings highlighted the importance of family-focused preventive interventions in mitigating the long-term effects of CT.

Ecologically significant shallow-water (0–30 m) marine animal forests in central New Zealand

Marine animal forests are habitats formed by dense aggregations of suspension-feeding invertebrates that are increasingly being recognised as biodiversity hotspots with key roles in ecosystem functioning. Despite being found across the world, these ecologically important ecosystems remain understudied in temperate seas. Furthermore, while temperate animal forests are typically associated with deeper water, they can also occur in the shallows (< 30 m). This study is the first to describe the diversity of shallow-water (0–30 m) marine animal forests in the Wellington Region of New Zealand using a combination of SCUBA, ROVs, and citizen science surveys. Through qualitative and quantitative surveys, we identified nine distinct biotic communities dominated by sessile invertebrates, including sponges, cnidarians, bivalves, brachiopods, and polychaetes. In Wellington Harbour, the most common communities were sponge beds formed by the massive sponge Suberites australiensis, often occurring with other sponges, the brachiopod Magasella sanguinea, and the bivalve Atrina zelandica. The S. australiensis beds occurred between 7 and 17 m, with densities up to 19 sponges per m2 and specimens up to 40 cm in diameter. Based on pumping rate measurements, we estimated the average filtration capacity of S. australiensis in the sponge beds to range between 1240 and 19,996 L m−2 day−1. Along Wellington’s open coasts, diverse sponge gardens were dominated by massive morphologies, such as Ecionema alata, together with fan, and branching sponges between 20 and 30 m deep. Community composition varied spatially, likely due to heterogeneity in currents, sedimentation, and food availability. Anthropogenic impacts on animal-dominated ecosystems in the harbour included widespread marine debris (e.g., plastics, fishing gear, and tyres), and substrate alteration from coastal development. The open-coast communities were comparatively undisturbed but might be vulnerable to anchoring and fishing activities. Our study revealed ecologically important marine animal forests in the shallow waters of Wellington, which are largely unprotected urban ecosystems. Conservation through mapping, monitoring, and strategic marine spatial planning is urgently needed to preserve these underappreciated biodiversity hotspots worldwide.

Soil properties predict below-ground community structure, but not nematode microbiome patterns in semi-arid habitats.

Microbial and microeukaryotic communities are extremely abundant and diverse in soil habitats where they play critical roles in ecosystem functioning and services that are essential to soil health. Soil biodiversity is influenced by above-ground (vegetation) and below-ground factors (soil properties), which together create habitat-specific conditions. However, the compound effects of vegetation and soil properties on soil communities are less studied or often focused on one component of the soil biota. Here, we integrate metabarcoding (16S and 18S rRNA genes) and nematode morphology to assess the effects of habitat and soil properties shaping microbial and microeukaryotic communities as well as nematode-associated microbiomes. We show that both vegetation and soil properties (soil bulk density) were major factors structuring microbial and microeukaryotic communities in semi-arid soil habitats. Despite having lower nutrients and lower pH, denser soils displayed significantly higher alpha diversity than less dense soils across datasets. Nematode-associated microbiomes have lower microbial diversity, strongly differ from soil microbes and are more likely to respond to microscale variations among samples than to vegetation or soil bulk density. Consequently, different nematode lineages and trophic groups are likely to display similar associated microbiomes when sharing the same microhabitat. Different microbiome taxa were enriched within specific nematode lineages (e.g. Mycobacterium, Candidatus Cardinium) highlighting potentially new species-specific associations that may confer benefits to their soil nematode hosts. Our findings highlight the importance of exploring above- and below-ground effects to assess community structure in terrestrial habitats, and how fine-scale analyses are critical for understanding patterns of host-associated microbiomes.

Enhanced Electrohydrogenation of Cis,Cis-Muconic Acid to Trans-3-Hexenedioic Acid Using Hybrid Metal/Carbon Fiber Cathodes

Organic electrosynthesis is a long-known technique for diverse product development and efficient synthesis. With the recent rise of biomass as an alternative carbon and energy source, electrochemistry is further gaining popularity due to its inherently green and environmentally benign operating conditions. Electrochemical hydrogenation (ECH) reactions are particularly attractive for biomass conversion due to their ability to abstract hydrogen from water, tolerate biogenic impurities, and utilize Earth-abundant base metal electrodes. Moreover, the rate and selectivity of the reaction can be tubed by controlling the electrode material, operating potential/current density, and reactor design. However, the implementation of this decarbonized technology for chemical production is still hampered by low productivity and, as a result, unfavorable economics. cis,cis-Muconic acid (ccMA), a bioderived C6 diunsaturated diacid platform molecule, can be converted into commodity chemicals like caprolactam, adipic acid (AA), and terephthalic acid, with AA being of particular interest for the synthesis of nylon 6,6.1 Apart from various drop-in chemicals, ccMA can also be electrochemically upgraded to trans-3-hexenedioic acid (t3HDA), a potential bio-advantaged substitute for AA as the additional C=C bond in t3HDA can be leveraged to synthesize performance-advantaged polyamides.2,3 In this work, we investigated the electrohydrogenation of ccMA to t3HDA over Pb cathodes. We demonstrate that the reaction follows a radical-based pathway involving sequential electron transfer and hydrogen addition in solution to selectively produce t3HDA (100% selectivity). Next, we contacted the cathode with carbon felts (CF) to increase productivity. CF are often used in fuel cells and H2O2 electrolyzers due to their excellent conductivity, porosity for enhanced turbulence, and ability to tune the gas-liquid-solid three-phase interface. Following similar principles, we placed a CF (3mm. thickness) in contact with the Pb cathode to lower the charge transfer resistance (~3Ω) at the interface. The surface functionalities of carbon allowed us to further tune the hydrophobicity/hydrophilicity of our hybrid electrode. Molecular dynamics (MD) calculations provided critical insights into the role of surface functionalities and guided the optimal functionalization of CF’s surface. Combining reactor configuration with fine-tuning of the electrode’s microenvironment, we were able to achieve a 50x increase in t3HDA productivity reaching up to 20 g/cm2/day at a current density of 200 mA/cm2. We further replaced the kinetically sluggish oxygen-evolution reaction with the glucose oxidation reaction at the anode, allowing us to operate the electrolyzer at a lower cell potential.Overall, ECH represents a valuable alternative to thermochemical transformations for biomass upgrading. This work provides critical insights into reactor modifications for the scaled-up synthesis of bio-derived performance monomers via hybrid microbial electrosynthesis (HMES).

Adverse childhood experiences and psychological maladjustment in adolescence: The protective role of epistemic trust, mentalized affectivity, and reflective functioning.

Adverse childhood experiences (ACEs) are psychosocial factors acknowledged as significant contributors to health consequences later in adolescence, including psychological maladjustment. The research suggests that, at a transdiagnostic and transtheoretical level, working on restoring epistemic trust (ET), mentalized affectivity (MA), and reflective functioning (RF) in adolescents with ACEs assumes a central role in the therapeutic process. However, there are still few studies that attempted to investigate the specific role of these sociocognitive factors in the detrimental positive association between levels of experienced ACEs during childhood and psychological maladjustment in nonclinical adolescents. A community sample of 306 Italian cisgender adolescents (61.4% assigned females at birth; age range 13-19 years old; Mage = 16.1, SD = 1.64) self-reported ACEs (Childhood Trauma Questionnaire-Short Form), psychological maladjustment (Strengths and Difficulties Questionnaire), ET (Epistemic Trust, Mistrust and Credulity Questionnaire), MA (Brief-Mentalized Affectivity Scale for Adolescence), and RF (Reflective Functioning Questionnaire). A multiple mediation regression analysis has been performed to explore the association between ACEs and psychological maladjustment through the interaction with socio-cognitive factors. ACEs are positive predictors of psychological maladjustment later in adolescence. Regardless of gender differences, epistemic mistrust and credulity, processing and expressing emotions, and uncertainty about mental states mediated this association, while epistemic trust, identifying emotions, and certainty about mental states did not configure as significant mediators. Findings suggest that ET disruptions and deficits in RF are risk factors, while MA is a protective factor within the link between ACEs and psychological maladjustment in adolescence. These links help to specify the role of sociocognitive factors in the development of mental problems in adolescents who have been exposed to adverse experiences in childhood.

Paternal Reflective Functioning &amp; Its Impact on Joint Play

Abstract Date Presented 03/23/24 Fathers hold a key role in their child’s development. This study explored the role of father-reflective functioning and masculinity views in joint play experience. Findings can inform OTs in developing family-centered services. Primary Author and Speaker: Julietta Gurgova Additional Authors and Speakers: Amiya Waldman-Levi, Amber Cope, Sara Haden

A Network Approach to Understanding the Role of Executive Functioning and Alpha Oscillations in Inattention and Hyperactivity-Impulsivity Symptoms of ADHD.

ADHD is a prevalent neurodevelopmental disorder characterized by symptoms of inattention and hyperactivity-impulsivity. Impairments in executive functioning (EF) are central to models of ADHD, while alpha-band spectral power event-related decreases (ERD) have emerged as a putative electroencephalography (EEG) biomarker of EF in ADHD. Little is known about the roles of EF and alpha ERD and their interactions with symptoms of ADHD. We estimated network models of ADHD symptoms and integrated alpha ERD measures into the symptom network. EF emerges as a bridge network node connecting alpha ERD and the hyperactivity/impulsivity and inattention symptoms. We found that EF most closely relates to a subset of symptoms, namely the motoric symptoms, "seat" (difficulty staying seated), and "runs" (running or climbing excessively). EF functions as a bridge node connecting alpha ERD and the ADHD symptom network. Motoric-type symptoms and EF deficits may constitute important nodes in the interplay between behavior/symptoms, cognition, and neurophysiological markers of ADHD.

The molecular mechanisms that underlie IGHMBP2-related diseases.

Immunoglobulin Mu-binding protein 2 (IGHMBP2) pathogenic variants result in the fatal, neurodegenerative disease spinal muscular atrophy with respiratory distress type 1 (SMARD1) and the milder, Charcot-Marie-Tooth (CMT) type 2S (CMT2S) neuropathy. More than 20 years after the link between IGHMBP2 and SMARD1 was revealed, and 10 years after the discovery of the association between IGHMBP2 and CMT2S, the pathogenic mechanism of these diseases is still not well defined. The discovery that IGHMBP2 functions as an RNA/DNA helicase was an important step, but it did not reveal the pathogenic mechanism. Helicases are enzymes that use ATP hydrolysis to catalyse the separation of nucleic acid strands. They are involved in numerous cellular processes, including DNA repair and transcription; RNA splicing, transport, editing and degradation; ribosome biogenesis; translation; telomere maintenance; and homologous recombination. IGHMBP2 appears to be a multifunctional factor involved in several cellular processes that regulate gene expression. It is difficult to determine which processes, when dysregulated, lead to pathology. Here, we summarise our current knowledge of the clinical presentation of IGHMBP2-related diseases. We also overview the available models, including yeast, mice and cells, which are used to study the function of IGHMBP2 and the pathogenesis of the related diseases. Further, we discuss the structure of the IGHMBP2 protein and its postulated roles in cellular functioning. Finally, we present potential anomalies that may result in the neurodegeneration observed in IGHMBP2-related disease and highlight the most prominent ones.

Loss of species and functions in a deforested megadiverse tropical forest.

Tropical species richness is threatened by habitat degradation associated with land-use conversion, yet the consequences for functional diversity remain little understood. Progress has been hindered by difficulties in obtaining comprehensive species-level trait information to characterize entire assemblages and insufficient appreciation that increasing land-cover heterogeneity potentially compensates for species loss. We examined the impacts of tropical deforestation associated with land-use heterogeneity on bird species richness, functional redundancy, functional diversity, and associated components (i.e., alpha diversity, species dissimilarity, and interaction strength of the relationship between abundance and functional dissimilarity). We analyzed over 200 georeferenced bird assemblages in the Atlantic Forest of Brazil. We characterized the functional role of the species of each assemblage and modeled biodiversity metrics as a function of forest cover and land-cover heterogeneity. Replacement of native Atlantic Forest with a mosaic of land uses (e.g., agriculture, pastures, and urbanization) reduced bird species richness in a nonrandom way. Core forest species, or species considered sensitive to edges, tended to be absent in communities in heterogenous environments. Overall, functional diversity and functional redundancy of bird species were not affected by forest loss. However, birds in highly heterogenous habitats were functionally distinct from birds in forest, suggesting a shift in community composition toward mosaic-exclusive species led by land-cover heterogeneity. Threatened species of the Atlantic Forest did not seem to tolerate degraded and heterogeneous environments; they remained primarily in areas with large forest tracts. Our results shed light on the complex effects of native forest transformation to mosaics of anthropogenic landscapes and emphasize the importance of considering the effects of deforestation and land-use heterogeneity when assessing deforestation effects on Neotropical biodiversity.

Surface Functionalization of Bamboo via Photo-Grafting Tannic Acid for Enhanced Silver Ion Loading Properties.

Photo-grafting is a gentle, simple, and precise approach to incorporating specific functional molecules for the surface functionalization of substrates. In this work, ultraviolet (UV)-induced tannic acid (TA) grafting onto the surface of bamboo was proposed as a viable strategy for functionalizing bamboo. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) clearly indicated that TA was successfully introduced to the bamboo's surface. The optimal conditions for the grafting reaction were determined to be 15 mM Methyl-2-benzoylbenzoate (BB), 30 mM TA, 20 min, and a pH = 8. Under these conditions, the amount of TA grafted onto the bamboo's surface was measured to be 19.98 μg/cm2. Results from Inductively Coupled Plasma (ICP) and Energy Dispersive Spectrometer (EDS) analyses showed that the silver ion loading capacity of tannic acid-grafted bamboo was significantly improved compared to that of raw bamboo and tannic acid-impregnated bamboo. Furthermore, the presence of TA grafted on the bamboo's surface exhibited a positive correlation with the loading of silver ions, indicating that grafted TA plays an important role in the surface functionalization of bamboo. We believe that photo-grafted TA may help generate multifunctional bamboo with diverse properties.

Gene body DNA hydroxymethylation restricts the magnitude of transcriptional changes during aging.

DNA hydroxymethylation (5hmC), the most abundant oxidative derivative of DNA methylation, is typically enriched at enhancers and gene bodies of transcriptionally active and tissue-specific genes. Although aberrant genomic 5hmC has been implicated in age-related diseases, its functional role in aging remains unknown. Here, using mouse liver and cerebellum as model organs, we show that 5hmC accumulates in gene bodies associated with tissue-specific function and restricts the magnitude of gene expression changes with age. Mechanistically, 5hmC decreases the binding of splicing associated factors and correlates with age-related alternative splicing events. We found that various age-related contexts, such as prolonged quiescence and senescence, drive the accumulation of 5hmC with age. We provide evidence that this age-related transcriptionally restrictive function is conserved in mouse and human tissues. Our findings reveal that 5hmC regulates tissue-specific function and may play a role in longevity.

DPP2/7 is a Potential Predictor of Prognosis and Target in Immunotherapy in Colorectal Cancer: An Integrative Multi-omics Analysis.

Colorectal cancer (CRC) ranks among the leading causes of cancerrelated deaths. This study aimed to illuminate the relationship between DPP7 (also known as DPP2) and CRC through a combination of bioinformatics and experimental methodologies. A multi-dimensional bioinformatic analysis on DPP7 was executed, covering its expression, survival implications, clinical associations, functional roles, immune interactions, and drug sensitivities. Experimental validations involved siRNA-mediated DPP7 knockdown and various cellular assays. Data from the Cancer Genome Atlas (TCGA) identified high DPP7 expression in solid CRC tumors, with elevated levels adversely affecting patient prognosis. A shift from the N0 to the N2 stage in CRC was associated with increased DPP7 expression. Functional insights indicated the involvement of DPP7 in cancer progression, particularly in extracellular matrix disassembly. Immunological analyses showed its association with immunosuppressive entities, and in vitro experiments in CRC cell lines underscored its oncogenic attributes. DPP7 could serve as a CRC prognosis marker, functioning as an oncogene and representing a potential immunotherapeutic target.