Intestinal Barrier Function Research Articles

Long non-coding RNA ANRIL/p65 negative feedback loop protects intestinal barrier function in inflammatory bowel disease.

Long non-coding RNA ANRIL/p65 negative feedback loop protects intestinal barrier function in inflammatory bowel disease.

Bazi Bushen Capsule Modulates Akkermansia muciniphila and Spermidine Metabolism to Attenuate Brain Aging in SAMP8 Mice.

Bazi Bushen Capsule Modulates Akkermansia muciniphila and Spermidine Metabolism to Attenuate Brain Aging in SAMP8 Mice.

Effects of Chinese herbal formula (including Houttuyniae Herba, Pulsatilliae Radix, Scutellariae Radix) supplementation in drinking water on growth performance, intestinal barrier function, and cecum microbiome composition of broilers under necrotic enteritis challenge.

Effects of Chinese herbal formula (including Houttuyniae Herba, Pulsatilliae Radix, Scutellariae Radix) supplementation in drinking water on growth performance, intestinal barrier function, and cecum microbiome composition of broilers under necrotic enteritis challenge.

Modulatory effects of polyherbal mixture on the immuno-antioxidant capacity and intestinal health of chicks infected with Escherichia coli O78.

A total of 180 one-day-old white-feathered broiler chicks were selected and randomly divided into 4 treatments, namely the control group (CON), Escherichia coli groups (E. coli), 2 g/kg polyherbal mixture group (PHM2), and the 4 g/kg polyherbal mixture group (PHM4). The CON and E. coli groups were fed a basal diet, while the PHM2 and PHM4 groups were fed the basal diet supplemented with 2 g/kg and 4 g/kg PHM, respectively. Each group had 3 replicates, with 15 broilers per replicate. On day 17 of the experiment, broilers in the E. coli, PHM2, and PHM4 groups were intraperitoneally injected with 0.8 mL of 1 × 108 CFU/mL of E. coli O78. Broilers in the control group received an equivalent volume of saline. Chicks were euthanized 48 h postinjection for collecting serum, liver, spleen, jejunum, ileum, ileal mucosa, and cecal contents. Our results showed that PHM significantly reversed the weight loss and decreased the diarrhea rate and the mortality of chicks caused by E. coli infection (P < 0.05). In the serum of chicks infected with E. coli, PHM significantly enhanced the antioxidant capacity (P < 0.05), increased the levels of immunoglobulins and anti-inflammatory cytokines (P < 0.05), and decreased the concentrations of proinflammatory cytokines (P < 0.05). Meanwhile, PHM also promoted the mRNA expression of antioxidant-related genes and decreased the expression of proinflammatory cytokines and apoptosis-related genes in the liver, spleen, jejunum, and ileum (P < 0.05). In addition, PHM repaired the intestinal barrier and injury to further reduce the serum concentrations of d-lactate (DAO) and lipopolysaccharide (LPS) (P < 0.05). More importantly, PHM significantly regulated the composition of cecal microbiota, especially by up-regulating the relative abundance of beneficial bacteria, including Faecalibacterium, Bacteroides, Butyricicoccus, and Lactobacillus, and down-regulating the relative abundance of pathogenic bacteria, including Enterococcus, Escherichia, and Shigella (P < 0.05). These beneficial bacteria were significantly positively correlated with antioxidant capacity and intestinal barrier function, while pathogenic bacteria were significantly positively correlated with proinflammatory cytokines (P < 0.05). In conclusion, PHM may be a potential preventive strategy for E. coli-infected poultry, which is closely related to its modulation of gut microbiota.

Targeted hydrolysis of α-lactalbumin to reduce allergenicity based on the synergistic effect of pepsin and papain.

Targeted hydrolysis of α-lactalbumin to reduce allergenicity based on the synergistic effect of pepsin and papain.

Poria cocos polysaccharides ameliorate AOM/DSS-induced colorectal cancer in mice by remodeling intestinal microbiota composition and enhancing intestinal barrier function.

Poria cocos polysaccharides ameliorate AOM/DSS-induced colorectal cancer in mice by remodeling intestinal microbiota composition and enhancing intestinal barrier function.

Interaction of osteopontin with α-lactalbumin and their synergistic effects in protecting intestinal barrier injury.

Interaction of osteopontin with α-lactalbumin and their synergistic effects in protecting intestinal barrier injury.

Influence of purslane extract on immuno-antioxidant status, intestinal barrier, and microbiota of chicks after experimental infection with Escherichia coli O78.

Influence of purslane extract on immuno-antioxidant status, intestinal barrier, and microbiota of chicks after experimental infection with Escherichia coli O78.

Hydrogel derived from decellularized pig small intestine submucosa boosted the therapeutic effect of FGF-20 on TNBS-induced colitis in rats via restoring gut mucosal integrity.

Hydrogel derived from decellularized pig small intestine submucosa boosted the therapeutic effect of FGF-20 on TNBS-induced colitis in rats via restoring gut mucosal integrity.

Diagnostic values of CD27, CD20 and MPO in pediatric ulcerative colitis.

Diagnostic values of CD27, CD20 and MPO in pediatric ulcerative colitis.

Microplastics bioaccumulation in fish: Its potential toxic effects on hematology, immune response, neurotoxicity, oxidative stress, growth, and reproductive dysfunction.

After being exposed, microplastics mostly bioaccumulated in guts and gills of fish, then, through circulation, spread and bioaccumulated in other tissues. Circulatory system of fish is impacted by the microplastic bioaccumulation in their tissues, influencing a number of hematological indices that are connected with immunity, osmotic pressure, blood clotting, molecular transport and fat metabolism. Variables like size, dose, duration, food consumption and species, all affect the bioaccumulation and toxicity of the microplastic, rather than the exposure routes. Microplastics lead to an imbalance in the generation of ROS and antioxidant defense of fish, which resulting in oxidative injury. Moreover, microplastics affect immunological responses through physico-chemical damage, hence produce neurotoxicity and modifies the activity of the acetylcholine esterase. Exposure to microplastics caused damage to the hepatic and gut tissue, affect intestinal barrier function and dysbiosis of microbial composition, altered the metabolism of host, affecting the activities of the digestive enzymes, eventually affecting the growth performance of fish. Microplastics exposure target the HPG axis and interfere with the process of steroidogenesis, apoptosis of the gonadal tissue, ultimately causing reproductive dysfunction. Fish exposed to microplastics have a range of toxic effects viz. alteration to immune, antioxidant and hematological indices, bioaccumulation, neurotoxicity, growth and reproductive dysfunction, all were examined in this present review by using different indicators.

Mapping the evolution of anti-diabetic polysaccharides research: Trends, collaborations, and emerging frontiers.

Mapping the evolution of anti-diabetic polysaccharides research: Trends, collaborations, and emerging frontiers.

Pdia3 deficiency exacerbates intestinal injury by disrupting goblet and Paneth cell function during ischemia/reperfusion.

Pdia3 deficiency exacerbates intestinal injury by disrupting goblet and Paneth cell function during ischemia/reperfusion.

A tri-compound formula comprising Ginsenoside Rg1, tetrandrine and icariin alleviates atopic dermatitis symptoms in a mouse model.

A tri-compound formula comprising Ginsenoside Rg1, tetrandrine and icariin alleviates atopic dermatitis symptoms in a mouse model.

The intestinal barrier: a pivotal role in health, inflammation, and cancer.

The intestinal barrier: a pivotal role in health, inflammation, and cancer.

AFB1 exacerbates testicular and intestinal inflammation by increasing stearoyl ethanolamide and homocysteine levels.

AFB1 exacerbates testicular and intestinal inflammation by increasing stearoyl ethanolamide and homocysteine levels.

Chronic fluoride induces neurotoxicity in zebrafish through the gut-brain axis.

Chronic fluoride induces neurotoxicity in zebrafish through the gut-brain axis.

Selenomethionine attenuates Klebsiella pneumoniae-induced jejunal injury in rabbits by inhibiting the TLR4/NF-κB pathway.

Selenomethionine attenuates Klebsiella pneumoniae-induced jejunal injury in rabbits by inhibiting the TLR4/NF-κB pathway.

Konjac glucomannan and κ-carrageenan synergistically contribute to the cecal intestinal barrier function of offspring via improving the benefits of maternal milk.

Konjac glucomannan and κ-carrageenan synergistically contribute to the cecal intestinal barrier function of offspring via improving the benefits of maternal milk.

Human placenta mesenchymal stromal cells alleviate intestinal inflammation and repair intestinal barrier function by activating AMPK-FXR pathway

Crohn’s disease (CD) has a complex pathogenesis; there is currently no effective treatment. Mesenchymal stromal cells (MSCs) are a potential therapeutic option for CD. It is important to systematically evaluate the safety and effectiveness of MSCs and their mechanism for the treatment of CD to support their clinical application. Here, the safety and effectiveness of MSCs from the human placenta (hPMSCs) are evaluated. All the indicators show that hPMSC transplantation is safe and effectively alleviates intestinal inflammation, reduces intestinal apoptosis and fibrosis, and promotes recovery of the intestinal barrier in mice and organoids. IGFBP-4 is the most expressed by hPMSCs and is verified to play a therapeutic role by activating the AMPK-FXR pathway to alleviate intestinal inflammation and repair the intestinal barrier function. Our findings demonstrate that hPMSCs alleviate CD by activating the AMPK-FXR pathway with IGFBP-4 to reduce intestinal inflammation and repair intestinal barrier function.