Full-thickness Wounds Research Articles

Starfish-Inspired Synergistic Reinforced Hydrogel Wound Dressing: Dual Responsiveness and Enhanced Bioactive Compound Delivery for Advanced Skin Regeneration and Management.

Effective wound management demands advanced dressings that protect while actively supporting healing. Traditional wound dressings often fall short of meeting the complex needs of skin repair. Inspired by the regenerative abilities of starfish, we developed a bionically engineered hydrogel designed to enhance wound healing. The hydrogel is synthesized through the coassembly of dopamine-modified cellulose nanofibers, chitosan, (3-aminobenzeneboronic acid)-grafted oxidized dextran, and poly(vinyl alcohol), utilizing dynamic Schiff base and boronic ester linkages. This innovative design imparts multifunctional properties, including injectability, 3D printability, antibacterial activity, self-adhesion, self-healing, antioxidant protection, and hemostasis, which emulate the defense mechanisms and regenerative processes of starfish. These characteristics work synergistically to reduce infection and oxidative stress and improve healing efficiency. Additionally, the hydrogel incorporates mangiferin and Vitamin C, which are released in a controlled manner in response to the wound's microenvironment (pH and reactive oxygen species), promoting tissue regeneration and reducing inflammation. In vitro tests confirmed its dual responsiveness, while finite element modeling validated the controlled release of bioactive compounds. In vivo testing on a rat full-thickness wound model showed a 100% healing rate by day 13, significantly outperforming commercial alternatives. The hydrogel's nontoxicity and advanced healing capabilities make it a promising solution for patients with critical healing needs, offering a comprehensive integration of natural biological processes and cutting-edge engineering.

Use of cadaveric skin allograft and Integra Dermal Regeneration Template to manage deep lower limb injuries.

In full-thickness wounds, it is necessary to have an appropriate dermal replacement because dermal tissue does not regenerate into normal dermis after injury. The use of a dermal matrix underneath a skin graft during the healing process provides a scaffold that supports tissue growth, resulting in improvement of cosmesis and functional outcomes. The management of large wounds with deep skin impairment using a combination of dermal matrices has not been exhaustively studied. The objective of this study was to evaluate the results of managing lower limb trauma, with deep skin impairment, by combining the use of dermal matrices in stages. This was a retrospective study of patients with lower limb trauma managed using a combination of cadaveric skin and Integra Dermal Regeneration Template (IDRT; Integra LifeSciences Corp., US) in stages, followed by an autologous skin graft, in the Hospital Aleman, Buenos Aires, Argentina from 2014-2021. Cosmesis was evaluated with the Vancouver Scar Scale (VSS) and Patient and Observer Scar Assessment Scale (POSAS). Functional outcomes were assessed one year after surgery. In total, five patients were treated. The average affected body surface area was 11.2%. The average cadaveric skin, IDRT and skin autograft take rates were 98.4%, 98.4% and 99%, respectively. Upon follow-up, six months after surgery, the mean VSS was 3.2 and the mean POSAS was 27.8. After 12 months, the mean VSS was 2.6 and the mean POSAS was 22.6. In addition, no depression of the covered surfaces was observed. All patients recovered full articular function and movement after physical therapy. All patients presented full wound coverage with satisfactory cosmesis and functional outcomes. The combination of the use of cadaveric skin and IDRT consecutively in the same wound bed provides promising results for the management of lower limb trauma wounds with deep skin impairment.

Niosome-Based Hydrogel of Quince Extract: A Promising Strategy for Expedited Full-thickness Wound Healing in Rat.

The regeneration of tissue damage involves a series of molecular and cellular events that can be mediated by various natural compounds. Recent studies have highlighted the anti-inflammatory, anti-ulcer, and skin-protecting properties of Cydonia oblonga (Quince), which are mainly attributed to phenolic compounds. These compounds may have some drawbacks when targeting wound applications, including low bioavailability at the wound site. Moreover, to overcome these limitations, surfactant-based nanovesicular systems have been developed as carriers of such compounds for wound healing. This study aimed to highlight the possible therapeutic potential of niosome-based hydrogel from Quince extract to stabilize and deliver the related bioactive compounds to full-thickness wounds in rats. The niosomal hydrogel was prepared using a thin-film hydration method with the fruit extract (70% methanol). The formulation was optimized by evaluating size, zeta potential, dispersion index, and drug encapsulation efficiency. Full-thickness wounds were created on the dorsal cervical area of Wistar rats, and histopathological analysis of biopsy specimens was conducted on the 12th day of treatment. Under the study conditions, niosomal hydrogel displayed good physicochemical stability. Histopathological findings demonstrated that niosomal gel promoted angiogenesis, fibroblast maturation, collagen deposition, keratinization, and epidermal layer formation more effectively than control and hydrogel base. Furthermore, niosomal gel treatment markedly reduced inflammation. The total phenol concentration was determined to be 13.34 ± 0.90 mg gallic acid equivalents per gram of dried extract. The niosomal hydrogel containing C. oblonga extract shows potential as a novel approach for wound healing, warranting further investigation in this field.

Design and characterization of a ROS-responsive antibacterial composite hydrogel for advanced full-thickness wound healing.

Design and characterization of a ROS-responsive antibacterial composite hydrogel for advanced full-thickness wound healing.

Dulaglutide accelerates diabetic wound healing by suppressing Nrf2-dependent ferroptosis in diabetic mice.

Dulaglutide accelerates diabetic wound healing by suppressing Nrf2-dependent ferroptosis in diabetic mice.

Nanocurcumin-Enhanced Zein Nanofibers: Advancing Macrophage Polarization and Accelerating Wound Healing

Nanocurcumin-Enhanced Zein Nanofibers: Advancing Macrophage Polarization and Accelerating Wound Healing

Oxidized hyaluronic acid/ε-polylysine/quaternized chitosan hydrogel with shear thinning injectability and self-healing properties for full-time and multipurpose wound healing.

Oxidized hyaluronic acid/ε-polylysine/quaternized chitosan hydrogel with shear thinning injectability and self-healing properties for full-time and multipurpose wound healing.

PH-responsive cationic guar gum-based multifunctional hydrogel with silver nanoenzymes: Combined photothermal antibacterial therapy and antioxidant properties for MRSA infected wound healing.

pH-responsive cationic guar gum-based multifunctional hydrogel with silver nanoenzymes: Combined photothermal antibacterial therapy and antioxidant properties for MRSA infected wound healing.

Retraction notice to "In vitro and in vivo assessment of curcumin-quercetin loaded multi-layered 3D-nanofibroporous matrix prepared by solution blow-spinning for full-thickness burn wound healing" [Int. J. Biol. Macromol. 270P1 (2024) 132269

Retraction notice to "In vitro and in vivo assessment of curcumin-quercetin loaded multi-layered 3D-nanofibroporous matrix prepared by solution blow-spinning for full-thickness burn wound healing" [Int. J. Biol. Macromol. 270P1 (2024) 132269

A thermosensitive chitin hydrogel with mild photothermal-chemotherapy for facilitating multidrug-resistant bacteria infected wound healing.

A thermosensitive chitin hydrogel with mild photothermal-chemotherapy for facilitating multidrug-resistant bacteria infected wound healing.

Zinc sulfate gel reshapes the wound microenvironment to promote full-thickness wound healing in mice.

Zinc sulfate gel reshapes the wound microenvironment to promote full-thickness wound healing in mice.

Recombinant collagen microneedles for transdermal delivery of antibacterial copper-DNA nanoparticles to treat skin and soft tissue infections.

Recombinant collagen microneedles for transdermal delivery of antibacterial copper-DNA nanoparticles to treat skin and soft tissue infections.

Chemical structure of antibiotics determines their release rate from drug-loaded poly(vinyl alcohol)/sodium sulfated alginate nanofibrous wound dressings.

Chemical structure of antibiotics determines their release rate from drug-loaded poly(vinyl alcohol)/sodium sulfated alginate nanofibrous wound dressings.

Conductive hyaluronic acid/phytanic acid hydrogel to deliver recombinant human amelogenin for diabetic wound repair.

Conductive hyaluronic acid/phytanic acid hydrogel to deliver recombinant human amelogenin for diabetic wound repair.

Retraction notice to "Accelerative effect of nanohydrogels based on chitosan/ZnO incorporated with citral to heal the infected full-thickness wounds; an experimental study" [Int. J. Biol. Macromol. 217 (2022) 42-54

Retraction notice to "Accelerative effect of nanohydrogels based on chitosan/ZnO incorporated with citral to heal the infected full-thickness wounds; an experimental study" [Int. J. Biol. Macromol. 217 (2022) 42-54

Study of Plastic Kaempferol Hydrogel for the Treatment of Hand Injuries Sustained During Rowing Training and Other Situations

The regular occurrence of hand injuries is a significant health issue in training, such as rowing. This research incorporated kaempferol components and developed a gel for protecting against trauma to prevent hand injuries, based on the author's expertise in training and protection. The gel is a hydrogel prepared by freezing and thawing polyvinyl alcohol (PVA). The gel provides a moist environment and cover protection for the wound, thereby accelerating wound healing. In addition, kaempferol possesses various biological activities, such as anti-inflammatory, anti-cancer, antibacterial, and antioxidant effects. Therefore, in this study, we used PVA and kaempferol to prepare kaempferol composite hydrogel and the effect of kaempferol hydrogel on cells was characterized. Kaempferol's antibacterial properties were confirmed, and the effectiveness of kaempferol composite hydrogel in healing full-thickness skin wounds in mice was studied. In addition, it proved that the new hydrogel has good tension and maintains a stable state at room temperature.

Enhancing wound healing with synthetic hyaluronic acid injection in sutured incisions on BALB/c mice

Introduction: Hyaluronic acid has been shown to possess notable properties in wound healing, skin regeneration, and anti-inflammatory effects. The formation of scar tissue is a common and unintended phenomenon during the wound-healing process, potentially leading to diverse consequences for individuals. Aim: The objective of this study was to investigate the effect of injecting synthetic hyaluronic acid on the wound healing process in BALB/c mice subjected to suture wounding. Materials and methods: In this experimental study, 18 adult male BALB/c mice were utilized and distributed into two groups (9 mice each): an experimental group and a control group. A full-thickness incision wound, one centimeter in length, was created in the skin of the back neck area for both groups. After being wounded, all wounds were sutured. However, only mice in the experimental group had 0.05 mL of hyaluronic acid on both sides of the wound. On days 7, 14, and 21 following the injection of hyaluronic acid, three mice from each group were anesthetized, and skin samples were collected for histological evaluation. After staining the tissue slides with hematoxylin-eosin and Masson’s trichrome, a comprehensive analysis was conducted by a pathologist to quantify key histological parameters, including the number of fibroblasts, collagen fiber density, granulation area, and epithelium thickness. Results: The experimental group demonstrated an increased number of fibroblasts, higher collagen density, and a more organized arrangement of collagen fibers when compared to the control group. On day 21, the experimental group exhibited a significantly higher average thickness of the epithelium and greater hair growth when compared to the control group. Conclusion: Our findings suggest that hyaluronic acid exerts a positive influence on the healing process of sutured incisions, positioning it as a promising candidate for preventing the formation of abnormal scars at the site.

Honey, Gellan Gum, and Hyaluronic Acid as Therapeutic Approaches for Skin Regeneration.

Background/Objectives: Chronic wounds pose a significant health concern, with their prevalence increasing due to various etiologies. The global aging population further contributes to this rise, placing a substantial burden on healthcare systems in developed countries. This work aimed to develop new therapeutic options in the form of creams and dressings based on honey, gellan gum, and hyaluronic acid for preventing and treating chronic wounds across all stages. Methods: To address this, after the formulations were developed, in vitro cytocompatibility was determined. To confirm biocompatibility, an ovine wound model was used: full-thickness excisional wounds were treated with three formulations, namely gellan gum and honey sponges (GG-HNY), gellan gum, honey and hyaluronic acid sponges (GG-HA-HNY) and a honey-based cream (cream FB002). Daily assessments, including visual evaluation and wound scoring, were conducted for 30 days. Following the study period, tissues were collected for histological analyses. Results: The macroscopic examination revealed that all therapeutic groups facilitated lesion closure. Lesion size reduction, granulation tissue disappearance, and scar tissue development were consistent across all groups, with the group receiving cream demonstrating an advanced stage of healing. All groups achieved substantial wound closure by day 30, with no significant differences. Histopathological analysis following ISO standards revealed that GG-HA-HNY had the lowest ISO score, indicating minimal reactivity and inflammation, which corroborated the cytocompatibility. Conclusions: Combining these insights with previous findings enhances our understanding of wound regeneration dynamics and contributes to refining therapeutic strategies for chronic wounds. The formulations were designed to balance therapeutic efficacy with cost-effectiveness, leveraging low-cost raw materials and straightforward production methods.

Uncovering Sex-Related Differences in Skin Macrophage Polarization During Wound Healing in Diabetic Mice.

Chronic wounds, such as diabetes-related foot ulcers, arise from delayed wound healing and create significant health and economic burdens. Macrophages regulate healing by shifting between pro- and anti-inflammatory phenotypes, known as macrophage polarization. Sex and diabetes can impair wound healing, but their influence on macrophage phenotype in skin tissue during wound healing remains unclear, which was investigated in this study using a novel two-sex diabetic mouse model. Diabetes was induced in male and female C57BL/6J mice using low-dose streptozotocin injections and high-fat diet feeding, with chow-fed mice as controls. After 18 weeks, each mouse received four circular full-thickness dorsal skin wounds. The macrophage phenotypes in wounded skin tissues at Day 0 and Day 10 post-wounding were analyzed using mass cytometry with manual gating and automated computational clustering. Male diabetic mice exhibited more severe hyperglycemia and insulin resistance compared to females. Although diabetic mice did not display delayed wound healing, male mice had a greater proportion of total macrophages than females, especially a higher proportion of pro-inflammatory matrix metalloproteinase-9 (MMP-9)+ macrophages and a lower proportion of anti-inflammatory adiponectin receptor 1 (AdipoR1)+ macrophages in male diabetic mice compared to females, indicating an imbalanced polarization towards a pro-inflammatory phenotype that could result in poorer wound healing. Interestingly, computational clustering identified a new pro-inflammatory, pro-healing phenotype (Ly6C+AdipoR1+CD163-CD206-) more abundant in females than males, suggesting this phenotype may play a role in the transition from the inflammatory to the proliferative stage of wound healing. This study demonstrated a significant sex-based difference in macrophage populations, with male diabetic mice showing a pro-inflammatory bias that may impair wound healing, while a unique pro-inflammatory, pro-healing macrophage population more abundant in females could facilitate recovery. Further research is needed to investigate the role of these newly identified phenotypes in regulating impaired wound healing.

The Impact of Decellularization Method on the Cytocompatibility and Wound Healing Capability of Human Amniotic Membrane.

The decellularized human amniotic membrane (dHAM) has been evaluated as a biomaterial forvarioustissue engineering applications, notably asa skin dressing for wound healing. The decellularization process alters the composition and structure of the extracellular matrix consequently influencing its characteristics. The aim of the present study was to comparatively evaluate dHAM-E and dHAM-S prepared by enzymatic and salt solution treatment respectively for their microstructure using scanning electron microscopy (SEM), in vitro biocompatibility with mesenchymal stem cells (MSCs), and regenerative capability in full-thickness wound model in mice. The SEM results revealed increased porosity in dHAM-S and better MSC adhesion and proliferation as compared to dHAM-E. Moreover, wound healing capability assessed at day 7 and day 14 by histological analysis of the regenerated tissues indicated that the dHAM treated groups achieved greater re-epithelialization and remodeling than the untreated group. However, dHAM-S treated samples presented a more remodeled regenerated skin than the other groups. Furthermore, gene expression analysis of the regenerated skin displayed a higher expression of anti-inflammatory, proliferation, and keratinization marker genes in the dHAM treated groups. Overall, it was found that dHAMs are compatible with MSCs and improve wound healing. However, clear differences were observed in the bioactivity of the two dHAMs.