Quality Of Fresh Semen Research Articles

Increasing sperm production and improving cryosurvival of semen in aged roosters after diet-supplemented with selenium in Thai native chickens.

Aging roosters typically exhibit subfertility with decreasing semen quality, whereas Thai native roosters reared in rural areas were raised for a longer duration than their usual lifespan. The present study therefore aimed to assess the effect of selenium supplementation as an antioxidative substance in diets to improve the semen cryopreservation of aged roosters. Semen samples were collected from young (n=20) and aged (n=20) Thai native roosters (Pradu Hang Dum) at 36 and 105 weeks of age when starting the experiment, respectively. They were fed diets in non-supplemented or supplemented selenium (0.75 ppm). Fresh semen quality and lipid peroxidation of fresh semen was evaluated before cryopreservation using the traditional liquid nitrogen vapor method. Post-thaw sperm quality and fertility potential were determined. Advancing age is unrelated to decreasing fresh semen quality (p > 0.05). However, lipid peroxidation in rooster semen depended on age, and the malondialdehyde (MDA) concentration increased in aged roosters (p < 0.05). Selenium supplementation in diets significantly decreased the MDA concentration and increased the sperm concentration (p < 0.05). In contrast, cryopreserved semen was affected by advancing rooster age, and selenium influenced sperm quality (p < 0.05). Younger roosters had higher post-thaw sperm quality and fertility potential than aged roosters (p < 0.05). Likewise, diet selenium supplements improved post-thaw sperm quality and fertility compared with the non-supplement group. Rooster's age does not influence the rooster sperm quality of fresh semen, while sperm cryotolerance and fertility were greater in young roosters than in aged roosters. However, those of aged roosters could be improved by dietary selenium supplementation.

Evaluation of simple strategies to reduce the use of antibiotics in equine fresh sperm

According to the World Health Organization in 2021, the development of antimicrobial resistances is considered one of the top ten global public health threats. Microbial load negatively affects sperm quality and preservation (Ortega-Ferrusola and Peña, Reproduction in Domestic Animals. 2009; 44(3):518-522), which implies the use of antibiotics in semen extenders. The objective of this study was to study the effect of three semen processing techniques on quality and microbial load of fresh equine sperm to evaluate the possibility of reducing or eliminating the prophylactic use of antibiotics in the future. Ten adult stallions were included (one ejaculate per male). To avoid environmental contamination during sperm collection and processing, a protocol that maximizes hygiene measures was implemented. Aliquots of each ejaculate were processed following 4 protocols: Simple Centrifugation (450g x 7 min) in extender with antibiotics (SC+, as control group), Simple Centrifugation in extender without antibiotics (SC-), Single-Layer Colloidal Centrifugation (300g x 20 min) in extender without antibiotic (CC-) and Filtration (SpermFilter®) in extender without antibiotics (F-). Sperm motility (total and progressive motility, TM and PM, respectively, %), viability (Alives, %), and microbial load (on three different culture media (Columbia 5% Sheep Blood Agar, COS, general purpose medium for non-fastidious and fastidiousmicroorganisms, Sabouraud Dextrose Agar, SDA, for yeasts and molds, and Schaedler vitamin K1 5% Sheep Blood Agar, SCH, for fastidious anaerobic bacteria) were evaluated after processing. Statistical analysis was performed by Kruskal-Wallis, ANOVA and post hoc tests (p<0.05). Results are expressed as mean values ± SD. No significant differences were found between the control (SC+) and other groups (SC-, CC- and F-), neither in sperm characteristics (61,32a ± 20,84, 59,13a ± 21,84, 63,19a ± 18,87 and 52,54a ± 21,08 for PM and 84,60a ± 9,21, 85,05a ± 9,14, 84,90a ± 13,5 and 77,35a ± 13,27 for ALIVES respectively) nor in microbial load (216,50a ± 504,41, 348,30a ± 792,99, 731,30a ± 2180,11 and 51,70a ± 64,64 for COS; 1,00a ± 3,16, 11,00a ± 31,43, 4,00a ± 6,99 and 1,00a ± 3,16 for SDA and 25,00a ± 57,59, 41,00a ± 60,45, 10,00a ± 18,26 and 26,00a ± 42,48 for SCH respectively). The present processing methods, in combination with management measures that prevent environmental contamination, maintained sperm quality and microbial load of equine fresh semen to the same extent as traditional sperm processing protocols with antibiotics. Acknowledgements: Authors thank P. Cuesta for statistical assistance. This research was funded by Madrid Institute for Rural, Agricultural and Food Research and Development (IMIDRA).

The quality of fresh and frozen semen Bali bull assessed from different ejaculate volume

Semen volume is the amount of ejaculate released by male Bali cattle during one ejaculation, which consists of spermatozoa and seminal plasma. The purpose of this study was to determine the effect of Bali cattle ejaculation volume on the quality of fresh and frozen semen. Semen collection was carried out using an artificial vaginal collection method. The collected semen was then evaluated with microscopic parameters: motility, viability, and abnormalities. Semen came from 7 male Bali bulls. The data obtained were analyzed by one-way ANOVA. The results showed that there was no significant difference (P>0.05) between the volume of Bali cattle ejaculation on the quality of fresh semen and also post thawing based on the parameters tested. The sperm concentration of Bali bulls has average 935.75 ± 80.25 x 106. The average percentage of fresh semen motility was 70.79±1.26 while that for frozen was 47.83±1.89. The average viability of fresh spermatozoa was 80.04±3.68 while frozen 52.87±0.67. The average abnormality value of fresh spermatozoa was 8.54±1.88 while frozen was 23.83±4.22. Based on these results, it was concluded that the different volume of ejaculate of Bali cattle did not affect the quality of fresh semen of Bali cattle, both fresh and frozen based on the parameters of motility, viability, and abnormalities.

Time of day modified the time required for semen collection with electroejaculation and slightly affected the quality of fresh semen in rams.

The aim was to compare some stress responses to electroejaculation (EE), and the quality of fresh semen, when ram semen is collected at dawn (06:00h), noon (12:00h), or evening (18:00h). Twelve Corriedale rams were used, and semen was collected from four rams at each study time on three different days, with a Latin-square design. The time required for EE, the number of vocalizations emitted, heart rate, and rectal temperature were recorded, and fresh semen was evaluated. The time required for EE was shorter at evening than at dawn and noon (399.3s, 480.6s, and 460.2s respectively; pooled SEM = 72.1; P = 0.03). The percentage of sperm with progressive motility was greater at noon than dawn (59.7% and 50.3%; pooled SEM = 5.8; P = 0.05). Curvilinear velocity was greater at dawn than evening (117.0µm/s and 95.5µm/s; pooled SEM = 7.1; P = 0.04), slow linear velocity was greater at evening than at dawn and noon (13.1µm/s, 9.3µm/s, and 8.5µm/s respectively; pooled SEM = 1.7, P = 0.05), and the slow average path velocity was greater at evening than dawn and noon (16.2µm/s, 11.7µm/s, and 10.8µm/s respectively; pooled SEM = 1.9, P = 0.05). In conclusion, the collection time modified the time required for electroejaculation and had only slight effects on the quality of fresh semen. Overall, the time of the day appears to have only slight effects on semen collection and quality.

Influence of Semen Collection Frequency and Seasonal Variations on Fresh and Frozen Semen Quality in Thai Native Roosters

This study aimed to determine the effects of the frequency of semen collection (once, twice, and thrice weekly) and seasonal variations on the fresh and frozen semen quality of Thai native roosters throughout the year. Data on temperature and humidity were collected and used to calculate the temperature-humidity index (THI). The average temperature and THI were lower in the winter than in the rainy season and the summer (p < 0.05). In contrast, the average relative humidity was not different among the seasons but was higher in the rainy season (p > 0.05). None of the fresh or frozen semen quality parameters were influenced by the frequency of semen collection, but the season did have an effect. The highest sperm concentration was obtained in the winter (p < 0.05). In contrast, the lowest sperm concentration was found during the rainy season, which presented the highest humidity. Regarding the frozen semen quality, the highest malondialdehyde concentration and the lowest motility were found in the summer (p < 0.05). In conclusion, semen collection can be conducted thrice per week for a consecutive year without affecting semen quality while maximizing sperm production. However, the highest sperm production was obtained in the winter, which is also a suitable season for producing semen for cryopreservation.

Optimized addition of nitric oxide compounds in semen extender improves post-thaw seminal attributes of Murrah buffaloes.

Semen dilution and cryopreservation alter the homogeneity of seminal plasma, resulting in a non-physiological redox milieu and consequently poor sperm functionality. Considering the concentration-specific bimodal action of nitric oxide (NO) in the regulation of sperm functions, cryopreservation media supplemented with optimized concentrations can improve the semen attributes. The present study aimed to evaluate the effect of adding an optimized concentration of sodium nitroprusside (SNP) and N-nitro-L-arginine methyl ester (L-NAME) in an extender on in vitro semen quality. An aliquot of semen samples (n = 32) from Murrah buffalo bulls (n = 8) was divided into control (C) and treatment (T-I: SNP in extender at 1µmol/L; T-II: L-NAME in extender at 10µmol/L). Fresh semen quality parameters showed no significant difference at 0h except for the structural integrity in the T-II group. Post-thaw semen quality parameters and sperm kinematics using computer-aided sperm analysis (CASA) revealed significantly higher (p < 0.05) cryoresistance in the treatment groups. Viability, acrosome integrity, and membrane integrity were significantly higher (p < 0.05) in both treatment groups; however, the results were pervasive in T-II. Lower abnormal spermatozoa were observed in both T-I and T-II. SNP supplementation led to a significant rise (p < 0.05) in NO, whereas L-NAME reduced the NO concentration in post-thawed samples, which was directly correlated with different sperm functionality and associated biomarkers viz. total antioxidant capacity (TAC) and thiobarbituric acid reactive substance (TBARS). It was concluded that the cryopreservation media supplemented with SNP and L-NAME at 1µmol/L and 10µmol/L, respectively, lower the cryo-damage and improve post-thaw seminal attributes.

Semen Quality Traits of Two Thai Native Chickens Producing a High and a Low of Semen Volumes

Semen quality traits such as semen volume, sperm motility, sperm concentration, pH, and color are very important, since they can determine the fertility potential of the semen. The objective of this study was to compare the semen quality traits of Thai Native chickens (Pradu Hang Dam and Chee) producing high and low semen volume. Semen was collected from 24 roosters (12 reps) and divided into two groups of roosters producing high semen volume (>0.3 mL) and those producing a low amount of semen (<0.3 mL). Fresh semen quality parameters (semen volume, sperm motility, viability, concentration, and pH) were measured and compared between groups. It was found that semen volume showed a positive correlation with sperm concentration, mass movement, motility, viability, and pH of fresh semen. There was no significant difference in fresh semen parameters between Pradu Hang Dam and Chee roosters with either high or low ejaculate semen, except for sperm concentration. Sperm concentration was significantly higher in Chee compared with Pradu Hang Dam in both high and low ejaculate semen. The semen quality parameters between groups of chickens producing high and low ejaculate semen were significantly different except for sperm viability. In conclusion, it was found that chickens producing high volumes of semen have better sperm quality than those producing a low semen volume. Therefore, these results provide a possible consideration that the amount of semen production among chickens can be used as a tool in selecting Thai native roosters for breeding.

The Quality of Fresh and Frozen Semen and its Correlation with Molecular Weight of Seminal Plasma Protein in Bali Cattle

This study evaluated the quality of fresh and frozen semen of Bali cattle and its correlation with the molecular weight (MW) of seminal plasma protein. This study collected semen from 10 bulls aged 5–10 years using an artificial vagina and evaluated the samples macroscopically and microscopically. Two batches of frozen semen obtained in 2020 and 2021 were also analyzed. The frozen semen samples were thawed at 37 °C for 30 seconds. The sperm motility, viability, intact plasma membrane (IPM), and sperm abnormalities were investigated. The concentration of the seminal plasma proteins was determined using the Bradford method, and the proteins were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-SDS-PAGE). Additionally, the gels were stained with Coomassie brilliant blue, and the MWs of the proteins were determined using MW markers. The sperm motility, viability, and abnormalities of fresh semen varied significantly among the bulls (p<0.05); however, sperm IPMs among the bulls were similar (p>0.05). No differences in sperm motility after freezing were found among the bulls. However, the sperm viability, abnormality, and IPM varied among the bulls. Meanwhile, the seminal plasma proteins contained bands with different MWs. No difference in the expression of protein bands between bulls. Linearity analysis showed that sperm motility (r= 0.281), viability (r= 0.189), abnormalities (r= 0.141), and IPM (r= 0.173) were positively correlated with the protein bands at each MW (p<0.05). The results conclude there was a positive correlation between the MW of the protein marker and the same protein expression levels in Bali bulls. Therefore, the band intensity of Bali cattle seminal plasma proteins can be used as a biomarker for selecting superior Bali bulls.

Profiling of key heat shock proteins and their relationship with male sexual behavior and seminal characteristics in Kankrej (Bos indicus) breeding bulls during different seasons.

The goal of this study is to use indirect ELISA to determine the concentration of major heat shock proteins (Hsps) in Kankrej (Bos indicus) breeding bulls and their relationship with certain male phenotypic traits including sexual behavior, sperm quality, and bull fertility in different seasons. The seasonal fluctuation in the concentration of three major Hsps (60, 70, and 90) was determined using an indirect enzyme-linked immunosorbent assay (ELISA). According to the findings, Hsps levels are significantly higher during the summer season and are associated with both fresh and post-thawed semen quality traits in Kankrej breeding bulls. The better sexual behavior of bulls and seminal parameters of fresh or thawed semen was observed in the winter season together with the lower concentrations of HSPs. These could suggest negative association between HSPs with bull sexual behavior and seminal parameters. As a result, the concentration of Hsps in breeding bulls may be a useful indicator for determining fertility traits.

Correlation between Semen Quality, Libido, and Testosterone Concentration in Bali Bulls

Indonesia has two National Artificial Insemination Centers (AIC) and more than 15 Regional Artificial Insemination Centers (RAIC) spread across several provinces. Bulls in the AIC must have a high libido and produce good quality semen. This study examines the correlation between libido with semen quality and testosterone concentration to determine potential frozen semen production from Bali bulls in South Sulawesi RAIC. Ten Bali bull were used in this study. Semen collection was carried out twice a week with semen evaluation following the RAIC protocol. At the same time, blood samples and libido measurements were carried out from each bull. The frozen semen production potential was calculated by multiplying the semen volume, motility, and sperm concentration. The results showed that the quality of fresh semen and testosterone concentrations did not differ between high and low libido of Bali bulls. Libido has a positive correlation with semen volume (r= 0.52) and sperm motility (r= 0.62), while testosterone concentration has a negative correlation with semen volume (r= -0.65), sperm motility (r= -0.60), and libido (r= -0.48). Bulls with high and low libido have good frozen semen production potential, ranging from 19,755 – 21,640 straws per year. Bali bulls in RAIC have fresh semen quality and testosterone concentrations under normal conditions, with high potential for frozen semen production, although only 60% of Bali cattle have high libido and 40% have low libido.

EFECTO DEL TIPO DE CRIOPROTECTOR Y TEMPERATURA DE EQUILIBRIO EN LA CRIOPRESERVACIÓN DE LOS ESPERMATOZOIDES DE ALPACA (Vicugna pacos)

The objective of this study was to determine the effect of glycerol (GL), dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) at temperatures of 5 °C and 16 °C of equilibrium on sperm quality, after cryopreservation. 6 males were used in the semen collection, motility parameters were evaluated with the CASA system, viability, acrosomal integrity and mitochondrial activity by flow cytometry in fresh and thawed semen. We collected 30 ejaculates of which 10 were discarded for not fulfilling (volume ≥ 1 ml and total motility ≥ 60 %), the 20 ejaculates were mesclo in a tube to form pool carrying out the assessment of the initial quality of fresh semen, obtaining a total motility (MT) 61,6 ± 3,4%, progressive motility (MP) 51,7 ± 3,7%, viability 78,1 ± 9.1%, mitochondrial activity (MA) 77,4 ± 7,7 and acrosomal integrity 88,9 ± 11,5%. After cryopreservation no equilibrium temperature effect was observed, independently of cryoprotectant in the parameters of seminal quality. Cryoprotective effect (P≤ 0.05) was observed, presenting superior values in the sperm conserved in GL with respect to the rest of cryoprotectants, with significant differences (P ≤ 0,05) in MT, MP, viability and AM and without significant differences in acrosomal integrity. The equilibrium temperature of 16 °C was effective for cryopreservation of alpaca sperm. In addition, the 5% GL preserved better the functional characteristics of sperm compared to 7% (DMF and DMSO).

The Quality of Bali Bull Sexed Sperm Using Soybean Extract Extender

The process of sexing spermatozoa requires a medium that is able to protect and provide an optimal environment. One of them is extender media that is used to extend the volume of semen. The extender that is commonly used is commercially but some limitation such as price and availability. Therefore, it is necessary to have an alternative extender such as soybean. The aim of this study was to know the quality of sexed sperms diluted using soybean extender. Semen of Bali bull was collected for five times and then subjected to three different extender treatments after sexing; T1 (Soybean), T2 (Tris), T3 (Tris-soybean), and T0 (Fresh semen-tris) was used as control before sexing. The parameters observed were the quality of fresh semen and after sexing. The results of the study showed that the characteristic of Bali bull fresh semen macroscopically in this study was 4.75 mL in volume, cream color, distinctive odor, pH 6.6, moderate consistency, and microscopically motility, viability, abnormality, and concentration were 94.22%, 96.06% 4.89, and 1596×106, respectively. Motility and viability after sexing were significantly (P&lt;0.05) decrease in comparison to the fresh semen (T0) at each treatment both top and bottom layers. In conclusion, the smallest decreased of the sperms motility and viability were still greater than 50%. Different extenders as one of the treatments in the present study did not showing different motility and viability of the sperms. This suggests that the use of tris and soybean or their combination can be used as semen extender for Bali bull sexed semen. Keywords: Soy extender, Bali bull, sexing, motility, viability.

Estimation of Genetic Variance Based on the Growth Differentiation Factor 9 (GDF9) Gene and its Association with Semen quality in Local Iraqi Goats

Growth differentiation factor 9 (GDF9) is a member of the TGFß superfamily that plays a critical role in females' ovarian follicular development and ovulation rate. However, its role in the testis has not been elucidated in Goats. Therefore, this study investigated the effects of GDF9 mutations on the quality of fresh semen in Bucks. Direct sequencing was used to screen possible SNP loci in the goat GDF9 exon one, one SNP locus site 2006 CTC &lt; CTA ( leu &lt; leu ) was identified positively in 15 local Iraqi Bucks. Analysis of variance revealed that significant effect of breeding value for concentration, individual ( p &gt; 0.05) movement, and Acrosome integrity, in All, so of dominance effects of volume, Individual movement, and Acrosome integrity. All traits have low genetic variation, which means that the environment varies high. This study is the first to show a significant association of GDF9 in the initiation or maintenance of spermatogenesis in male goats.

The Effect of Different rearing Systems on Microscopic Quality of Fresh Semen in Thin Tail Sheep

The rearing system is one of the factors that determine livestock productivity. Good productivity can be influenced by the reproduction of livestock including male livestock, namely the quality of semen. Factors affecting the semen quality of thin-tailed sheep need to be considered, one of which is the livestock rearing system. This study aims to determine the microscopic quality of fresh semen of thin-tailed sheep raised with different rearing systems. The results showed that the best microscopic quality of fresh thin-tailed sheep semen was in intensive livestock rearing at parameters 80.31% motility, +++ mass movement, and 33.84x108 spermatozoa/ml concentration. Keywords: Microscopic Quality, rearing system, thin tail sheep.

Comparison of single layer centrifugation and magnetic activated cell sorting for selecting viable boar spermatozoa after thawing

Sperm selection techniques, such as magnetic activated cell sorting (MACS) and colloid centrifugation, are reported to select good quality spermatozoa from semen samples of various species. Although the sperm quality of fresh boar semen is usually good, cryopreservation has a negative effect on parameters such as plasma membrane integrity and mitochondrial activity. Therefore, the objective of the present study was to determine whether MACS or centrifugation through a single layer of colloid (Single Layer Centrifugation, SLC) would be beneficial in enriching thawed boar sperm samples for viable spermatozoa with active mitochondria and good chromatin integrity. Frozen samples from three boars, three ejaculates per boar, were thawed and split. One part was selected by MACS, one was prepared by SLC, and the remainder served as the control. Controls and the selected sperm samples were evaluated for sperm quality (plasma membrane integrity, chromatin integrity, mitochondrial membrane potential and production of reactive oxygen species). Although several aspects of sperm quality were improved in the SLC-selected sperm samples compared to control, the flow-through MACS samples were only improved in having a lower proportion of spermatozoa with immature chromatin (Hi green fluorescence) compared to the labeled control. Sperm quality in the SLC samples was better than in the flow-through samples from MACS. Therefore, despite promising reports of the use of MACS for selecting good quality spermatozoa from semen in other species, the method was not useful for improving sperm quality in the thawed boar sperm samples in this experiment.

Karakteristik dan kualitas semen sapi Sumba Ongole dalam pengencer tris yang disuplementasi dengan susu skim pada suhu 3-5 ºC

The aims of this study were to determine the characteristics and quality of fresh semen from Sumba Ongole (SO) bulls which were diluted with tris dilution supplemented with skim milk. The study used a completely randomized design consisting of 4 treatments and 5 replications. The treatments were P0 = 100 % Tris, P1 = 88 % Tris + 12 % Susu skim, P2 = 86 % Tris + 14 % Susu skim, dan P3 = 84 % Tris + 16 % Susu skim. The parameters observed were the colour, smell, consistency, pH, volume, motility, viability, and abnormalities of semen. The results showed that the characteristics of the semen were normal, the color of the semen was creamy white, the semen volume was 4.6 ml, the consistency was moderate, pH 6.3, the smell was typical of SO cattle, the motility was 80%, the mass movement was +++, the concentration was 758 million/ ml, viability is 74.24% and abnormality is 16.12%. The characteristics and quality of cement in the four treatments showed no significant difference (P&gt;0.01). The motility and viability of spermatozoa in each treatment were able to survive up to day 3. The Tris diluent with the addition of skim milk was not able to maintain the characteristics and quality of the semen.

2. Effect of the Addition of Palm Kernel and Ammoniated Lemongrass Waste (Cymbopogon Nardus) on the Quality of Fresh Semen of Thin Tailed Sheep as a Partial Replacement of Basal Feed

In this study, three sheep aged two years old with a bodyweight of 20-25 kg were used. The treatments consisted of R1 (10% palm kernel cake and ammoniated citronella waste), R2 (20% oil palm kernel cake and ammoniated citronella waste) and R3 (30% oil palm kernel cake and ammoniated citronella waste). Research design used was Latin Square Design with three treatments and three replications. The parameters of semen observed were macroscopic and microscopic quality which included color, odor, volume, consistency, pH, concentration, mass movement and individual motility. The results showed that the administration of palm kernel cake with concentrations of 10%, 20%, and 30% and ammoniated citronella waste had no significant effect (P0.05) on the quality of fresh thin-tailed sheep's semen both macroscopically and microscopically. The semen had an average volume of 0.62 ml, creamy in color, had a characteristic as semen odor, thick in consistency and has a normal pH of 6.89. Microscopically, it had average concentration of 1809x106/ml, average mass movement of ++, and average individual motility of 57.57%. Based on the results, the administration of palm kernel cake and ammoniated citronella waste does not affect the quality of the fresh semen of thin-tailed sheep, both macroscopically and microscopically.

Growth performance, reproductive parameters and fertility measures in young Nellore bulls with divergent feed efficiency.

The growth, sexual maturity and fertility-related parameters related of young Nellore bulls with divergent residual feed intake (RFI) raised on pasture were evaluated. After classification of 48 young males as low and high RFI (more and less efficient, respectively), the animals were evaluated for growth and reproductive parameters at 28-day intervals from 14.3 to 24.6 months of age. The semen was cryopreserved in the last sampling and fresh and post-thaw semen samples were evaluated. Low RFI bulls exhibited higher initial and final body weight (P < 0.05), but feed intake, body condition score and growth measures evaluated by carcass ultrasound were unaffected by RFI (P > 0.05). The scrotal circumference, sperm concentration, defects, and quality of fresh semen, and ultrasonographic testicular characteristics were unaffected by RFI (P > 0.05). However, velocity parameters such as average path and curvilinear velocities determined by computer-assisted sperm analysis of thawed semen submitted to the rapid thermoresistance test were improved (P < 0.05) in low RFI bulls, but this improvement in quality did not enhance in vitro sperm fertilizing ability. Our results demonstrated significant differences in metabolism and growth performance between bulls of divergent RFI. In addition, there was slight improvement in the semen quality of bulls with low RFI bulls, but this did not enhance in vitro fertilizing ability. Selection of beef bulls for RFI can be performed, which will result in economic benefits by improving the growth performance of the animals without affecting reproductive parameters.

Effects of Bull Age on the Fresh and Frozen Semen Quality of Aceh Cattle

Effects of Bull Age on the Fresh and Frozen Semen Quality of Aceh Cattle

Comparative Study on Semen Quality and Fertility of Red Chittagong Cattle, BCB1 and Munshiganj Bulls of Bangladesh

The present study was conducted to analyze the fresh and post-thaw semen quality and fertility from native bulls of Red Chittagong Cattle (RCC), BLRI Cattle Breed 1 (BCB1), and Munshiganj Cattle of Bangladesh. One hundred and seventy-two ejaculates were collected by artificial vagina set and semen analysis was performed using Computer Assisted Sperm Analyzer (CASA) at Bangladesh Livestock Research Institute. Commercial extender (AndroMed) was used to dilute the fresh semen. After equilibration (4°C for 4 hr), freezing was done using a programmable bio-freezer. Post-thawed semen was evaluated for sperm motility and kinematics. Cryopreserved semen straws were used for artificial insemination (AI) and determined the bull fertility based on 60 days non-return rate. Motility of the sperm differs significantly (p 0.05) among the bulls but the highest concentration was found in Munshiganj bull (1669.60 ± 192.07 million/ml) followed by RCC (1648.70 ± 91.07 million/ml) and BCB1 bull (1481.60 ± 167.35 million/ml). Moreover, the highest bent tail (5.89 ± 0.75%), coiled tail (1.01% ± 0.22%) and distal mid-piece reflex (2.26% ± 0.28%) were observed in BCB1 followed by Munshiganj and RCC. Amplitude of lateral head displacement (ALH) was recorded higher in post-thaw than in fresh semen. Kinematics parameters of post-thaw semen decreased than fresh semen irrespective of genotypes. More number of doses/ejaculates can be produced from Munshiganj bull (394.34 ± 127.95) followed by RCC (349.01 ± 120.91) and BCB 1 bulls (331 ± 98.99). Fertility rate does not differ among the bulls (p > 0.05) but the highest value was found for RCC (62.06% ± 1.94%) bulls. Therefore, it can be concise that, the quality of Red Chittagong Cattle semen is better than BCB 1 and Munshigang bull.